P58028 (AOFB_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amine oxidase [flavin-containing] B
EC=1.4.3.4

Alternative name(s):
Monoamine oxidase type B
Short name=MAO-B

Gene names

Name:

MAOB

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine By similarity.
Catalytic activity	RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.
Cofactor	FAD.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: Compara mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 520	519	Amine oxidase [flavin-containing] B		PRO_0000099858
Regions
	Topological domain	2 – 489	488	Cytoplasmic By similarity
	Transmembrane	490 – 516	27	Helical; Anchor for type IV membrane protein; By similarity
	Topological domain	517 – 520	4	Mitochondrial intermembrane By similarity
	Compositional bias	36 – 52	17	Arg/Lys-rich (basic)
Sites
	Site	156	1	Important for catalytic activity By similarity
	Site	365	1	Important for catalytic activity By similarity
	Site	382	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	52	1	N6-acetyllysine By similarity
	Modified residue	397	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58028 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0AEBAE99A48BC206	FASTA	520	58,410
	10 20 30 40 50 60 MNSKCDVVVV GGGISGLAAA KLLHDSGLNV VVLEARDCVG GRTYTLRNQN VKYVDLGGAY 70 80 90 100 110 120 VGPTQNRILR LAKELGLETY RVNDVERQIH HVKGKSYPFR GPFPPAWNPI SYLDHNNLWR 130 140 150 160 170 180 TMDDMGKEIP SDAPWKAPLA EEWDHMTMKE LLNKICWTNC PRQFGTLFVN LCFTAETHEV 190 200 210 220 230 240 SALWFLWYVK QCGGTTRIIS TTNGGQERKF VGGSGQISER IMNLLGDRVK LQRPVVYIDQ 250 260 270 280 290 300 TGESVLVETL NHEIYEAKYV ISAIPPALGM KIHFKPPLPM MKNQLVSRVP LGSVIKCIVY 310 320 330 340 350 360 YKDPFWRKKD FCGTMVIEGE EAPVLYTMDD TKPDGSYAAI IGFIAAHKAR KLARLTKEER 370 380 390 400 410 420 LKKLCELYAK VLGSKEALKP VHYEEKNWCE EQYSGGCYTA YFPPGIMTQY GRFLRQPVGR 430 440 450 460 470 480 IFFAGTETAT HWSGYMEGAV EAGERAAREV LNAIGKIPED EIWQPEPESV DVPAQPITTT 490 500 510 520 FLERHLPSVP GLLRLIRLTT VVSAVALGFL AQKRGLLLRI

References

[1]	"Nucleotide sequences of putative cDNAs for guinea-pig monoamine oxidase." Yaekashiwa N., Tamate H.B., Takeuchi T., Sugimoto H., Shibata K., Kinemuchi H. Inflammopharmacology 11:145-154(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain and Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB047271 mRNA. Translation: BAB40418.1.
RefSeq	NP_001166452.1. NM_001172981.1.
3D structure databases
ProteinModelPortal	P58028.
SMR	P58028. Positions 4-496.
ModBase	Search...
Protein-protein interaction databases
STRING	10141.ENSCPOP00000000879.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100135573.
Organism-specific databases
CTD	4129.
Phylogenomic databases
eggNOG	COG1231.
HOGENOM	HOG000221615.
HOVERGEN	HBG004255.
InParanoid	P58028.
OrthoDB	EOG412M55.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002937. Amino_oxidase. IPR001613. Flavin_amine_oxidase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOFB_CAVPO

Accession

Primary (citable) accession number: P58028

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program