Reviewed, UniProtKB/Swiss-Prot P58028 (AOFB_CAVPO)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] B
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type B
      Short name=MAO-B

Gene names

Name:

MAOB

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine By similarity.
Catalytic activity	RCH(2)NHR' + H(2)O + O(2) = RCHO + R'NH(2) + H(2)O(2).
Cofactor	FAD.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 520	519	Amine oxidase [flavin-containing] B		PRO_0000099858
Regions
	Topological domain	2 – 489	488	Cytoplasmic By similarity
	Transmembrane	490 – 516	27	Anchor for type IV membrane protein By similarity
	Topological domain	517 – 520	4	Mitochondrial intermembrane By similarity
	Compositional bias	36 – 52	17	Arg/Lys-rich (basic)
Sites
	Site	156	1	Important for catalytic activity By similarity
	Site	365	1	Important for catalytic activity By similarity
	Site	382	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	52	1	N6-acetyllysine By similarity
	Modified residue	397	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58028-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0AEBAE99A48BC206	FASTA	520	58,410
	10 20 30 40 50 60 MNSKCDVVVV GGGISGLAAA KLLHDSGLNV VVLEARDCVG GRTYTLRNQN VKYVDLGGAY 70 80 90 100 110 120 VGPTQNRILR LAKELGLETY RVNDVERQIH HVKGKSYPFR GPFPPAWNPI SYLDHNNLWR 130 140 150 160 170 180 TMDDMGKEIP SDAPWKAPLA EEWDHMTMKE LLNKICWTNC PRQFGTLFVN LCFTAETHEV 190 200 210 220 230 240 SALWFLWYVK QCGGTTRIIS TTNGGQERKF VGGSGQISER IMNLLGDRVK LQRPVVYIDQ 250 260 270 280 290 300 TGESVLVETL NHEIYEAKYV ISAIPPALGM KIHFKPPLPM MKNQLVSRVP LGSVIKCIVY 310 320 330 340 350 360 YKDPFWRKKD FCGTMVIEGE EAPVLYTMDD TKPDGSYAAI IGFIAAHKAR KLARLTKEER 370 380 390 400 410 420 LKKLCELYAK VLGSKEALKP VHYEEKNWCE EQYSGGCYTA YFPPGIMTQY GRFLRQPVGR 430 440 450 460 470 480 IFFAGTETAT HWSGYMEGAV EAGERAAREV LNAIGKIPED EIWQPEPESV DVPAQPITTT 490 500 510 520 FLERHLPSVP GLLRLIRLTT VVSAVALGFL AQKRGLLLRI

References

[1]	"Nucleotide sequences of putative cDNAs for guinea-pig monoamine oxidase." Yaekashiwa N., Tamate H.B., Takeuchi T., Sugimoto H., Shibata K., Kinemuchi H. Inflammopharmacology 11:145-154(2003) [PubMed: 15035816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain and Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047271 mRNA. Translation: BAB40418.1.
3D structure databases
HSSP	HSSP built from PDB template 1GOS based on UniProtKB P27338.
SMR	P58028. Positions 3-501.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P58028.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOFB_CAVPO

Accession

Primary (citable) accession number: P58028

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)