Reviewed, UniProtKB/Swiss-Prot P58027 (AOFA_CANFA)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] A
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type A
      Short name=MAO-A

Gene names

Name:

MAOA

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine By similarity.
Catalytic activity	RCH(2)NHR' + H(2)O + O(2) = RCHO + R'NH(2) + H(2)O(2).
Cofactor	FAD By similarity.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic sideBy similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism Neurotransmitter degradation
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Amine oxidase [flavin-containing] A		PRO_0000099848
Regions
	Topological domain	1 – 497	497	Cytoplasmic Potential
	Transmembrane	498 – 518	21	Anchor for type IV membrane protein Potential
	Topological domain	519 – 527	9	Mitochondrial intermembrane Potential
Sites
	Site	335	1	Important for substrate specificity By similarity
	Site	374	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	406	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58027-1 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 3F2C3FF5CED937C1	FASTA	527	60,088
	10 20 30 40 50 60 MASREKTSIE GHMFDVVVIG GGISGLSAAK LLAEHEVDVL VLEARDRVGG RTYTVRNEHV 70 80 90 100 110 120 DYVDVGGAYV GPTQNRILRL SKELGLETYK VNVNERLVQY VKGKTYPFRG AFPPVWNPIA 130 140 150 160 170 180 YLDYNNLWRT MDNMGKEIPA DAPWEAPHAE EWDKMTMKDL IDKICWTKTA RRFASLFVNI 190 200 210 220 230 240 NVTSEPHEVS ALWFLWYVKQ CGGTTRIFSV TNGGQERKFV GGSGQVSERI MERLGDRVKL 250 260 270 280 290 300 KRPVTYVDQS DDNIIIETLN HELYECKYVI SAIPPTLTAK IHFRPELPSE RNQLIQRLPM 310 320 330 340 350 360 GAIIKCMMYY KEAFWKKKDY CGCMIIEDEE APISITLDDT KPDGSLPAIM GFILARKADR 370 380 390 400 410 420 LAKLHKEIRK RKICELYAKV LGSQEALQPV HYEEKNWCEE QYSGGCYTAY FPPGIMTHYG 430 440 450 460 470 480 RVIRQPFGRI YFAGTETATH WSGYMEGAVE AGERTAREVL NALGRVAEKD LKTQEPESKD 490 500 510 520 VPAMEITHTF WERNLPSVTG LLKLIGFTTS VTALWIVAYK FRLLRRS

References

[1]	Hashizume C., Mori Y. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Beagle. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB038563 mRNA. Translation: BAB40325.1.
RefSeq	NP_001002969.1. XP_855548.1.
UniGene	Cfa.151
3D structure databases
HSSP	HSSP built from PDB template 1O5W based on UniProtKB P21396.
SMR	P58027. Positions 23-509.
ModBase	Search...
Genome annotation databases
Ensembl	ENSCAFG00000014435. Canis familiaris. [Contig view]
GeneID	403450.
KEGG	cfa:403450.
Phylogenomic databases
HOVERGEN	P58027.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

AOFA_CANFA

Accession

Primary (citable) accession number: P58027

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	November 4, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)