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Protein

CD59B glycoprotein

Gene

Cd59b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore (By similarity).By similarity

GO - Molecular functioni

  1. complement binding Source: GO_Central

GO - Biological processi

  1. cell activation Source: GO_Central
  2. cell surface receptor signaling pathway Source: GO_Central
  3. negative regulation of activation of membrane attack complex Source: MGI
  4. negative regulation of complement activation Source: MGI
  5. negative regulation of cytolysis Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_294181. Regulation of Complement cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
CD59B glycoprotein
Alternative name(s):
MAC-inhibitory protein
Short name:
MAC-IP
Membrane attack complex inhibition factor
Short name:
MACIF
Protectin
CD_antigen: CD59
Gene namesi
Name:Cd59b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1888996. Cd59b.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of external side of plasma membrane Source: MGI
  2. cell surface Source: MGI
  3. extracellular region Source: GO_Central
  4. extracellular space Source: MGI
  5. extracellular vesicular exosome Source: MGI
  6. focal adhesion Source: MGI
  7. plasma membrane Source: GO_Central
  8. vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 10481CD59B glycoproteinPRO_0000036114Add
BLAST
Propeptidei105 – 12925Removed in mature formSequence AnalysisPRO_0000036115Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 49By similarity
Disulfide bondi29 ↔ 36By similarity
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi42 ↔ 62By similarity
Disulfide bondi68 ↔ 86By similarity
Disulfide bondi87 ↔ 92By similarity
Lipidationi104 – 1041GPI-anchor amidated asparagineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiP58019.

PTM databases

PhosphoSiteiP58019.

Expressioni

Tissue specificityi

Widely expressed in the kidneys, brain, lungs, spleen and testis (PubMed:11471050) Testis specific (PubMed:10946279).2 Publications

Gene expression databases

CleanExiMM_CD59B.
ExpressionAtlasiP58019. baseline and differential.
GenevestigatoriP58019.

Structurei

3D structure databases

SMRiP58019. Positions 24-95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 10784UPAR/Ly6Add
BLAST

Sequence similaritiesi

Contains 1 UPAR/Ly6 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG83475.
GeneTreeiENSGT00390000016309.
HOGENOMiHOG000232180.
HOVERGENiHBG005284.
InParanoidiP58019.
KOiK04008.
OMAiNKAAFSI.
PhylomeDBiP58019.
TreeFamiTF338524.

Family and domain databases

InterProiIPR027101. CD59_glyco.
IPR016054. LY6_UPA_recep-like.
IPR001526. LY6_UPAR.
[Graphical view]
PANTHERiPTHR10036:SF6. PTHR10036:SF6. 1 hit.
PfamiPF00021. UPAR_LY6. 1 hit.
[Graphical view]
SMARTiSM00134. LU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAQRGLILL LLLLAVFCST AVSLKCYNCL DPVSSCKINT TCSPNLDSCL
60 70 80 90 100
YAVAGRQVYQ QCWKLSDCNS NYIMSRLDVA GIQSKCCQWD LCNKNLDGLE
110 120
EPNNAETSSL RKTALLGTSV LVAILKFCF
Length:129
Mass (Da):14,227
Last modified:October 3, 2012 - v2
Checksum:i3B80648C8C30F635
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 323LDP → FQF no nucleotide entry (PubMed:10946279).Curated
Sequence conflicti90 – 901D → G no nucleotide entry (PubMed:10946279).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292401 Genomic DNA. Translation: AAL04434.1.
BX640578, BX813317 Genomic DNA. Translation: CAM18975.1.
BX813317, BX640578 Genomic DNA. Translation: CAM27769.1.
BC139203 mRNA. Translation: AAI39204.1.
BC145912 mRNA. Translation: AAI45913.1.
BC171969 mRNA. Translation: AAI71969.1.
CCDSiCCDS16486.1.
RefSeqiNP_862906.1. NM_181858.1.
XP_006499865.1. XM_006499802.2.
XP_006499866.1. XM_006499803.2.
XP_006499867.1. XM_006499804.2.
XP_006499868.1. XM_006499805.2.
UniGeneiMm.359026.

Genome annotation databases

EnsembliENSMUST00000090429; ENSMUSP00000087912; ENSMUSG00000068686.
ENSMUST00000111130; ENSMUSP00000106760; ENSMUSG00000068686.
ENSMUST00000129749; ENSMUSP00000117553; ENSMUSG00000068686.
GeneIDi333883.
KEGGimmu:333883.
UCSCiuc008ljn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292401 Genomic DNA. Translation: AAL04434.1.
BX640578, BX813317 Genomic DNA. Translation: CAM18975.1.
BX813317, BX640578 Genomic DNA. Translation: CAM27769.1.
BC139203 mRNA. Translation: AAI39204.1.
BC145912 mRNA. Translation: AAI45913.1.
BC171969 mRNA. Translation: AAI71969.1.
CCDSiCCDS16486.1.
RefSeqiNP_862906.1. NM_181858.1.
XP_006499865.1. XM_006499802.2.
XP_006499866.1. XM_006499803.2.
XP_006499867.1. XM_006499804.2.
XP_006499868.1. XM_006499805.2.
UniGeneiMm.359026.

3D structure databases

SMRiP58019. Positions 24-95.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP58019.

Proteomic databases

PRIDEiP58019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090429; ENSMUSP00000087912; ENSMUSG00000068686.
ENSMUST00000111130; ENSMUSP00000106760; ENSMUSG00000068686.
ENSMUST00000129749; ENSMUSP00000117553; ENSMUSG00000068686.
GeneIDi333883.
KEGGimmu:333883.
UCSCiuc008ljn.1. mouse.

Organism-specific databases

CTDi333883.
MGIiMGI:1888996. Cd59b.

Phylogenomic databases

eggNOGiNOG83475.
GeneTreeiENSGT00390000016309.
HOGENOMiHOG000232180.
HOVERGENiHBG005284.
InParanoidiP58019.
KOiK04008.
OMAiNKAAFSI.
PhylomeDBiP58019.
TreeFamiTF338524.

Enzyme and pathway databases

ReactomeiREACT_294181. Regulation of Complement cascade.

Miscellaneous databases

NextBioi400074.
PROiP58019.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CD59B.
ExpressionAtlasiP58019. baseline and differential.
GenevestigatoriP58019.

Family and domain databases

InterProiIPR027101. CD59_glyco.
IPR016054. LY6_UPA_recep-like.
IPR001526. LY6_UPAR.
[Graphical view]
PANTHERiPTHR10036:SF6. PTHR10036:SF6. 1 hit.
PfamiPF00021. UPAR_LY6. 1 hit.
[Graphical view]
SMARTiSM00134. LU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and functional characterization of a new gene encoding the mouse terminal complement inhibitor CD59."
    Qian Y.-M., Qin X., Miwa T., Sun X., Halperin J.A., Song W.-C.
    J. Immunol. 165:2528-2534(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY.
    Strain: 129/Sv.
  2. "Genomic structure, functional comparison, and tissue distribution of mouse Cd59a and Cd59b."
    Qin X., Miwa T., Aktas H., Gao M., Lee C., Qian Y.M., Morton C.C., Shahsafaei A., Song W.C., Halperin J.A.
    Mamm. Genome 12:582-589(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/Sv.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiCD59B_MOUSE
AccessioniPrimary (citable) accession number: P58019
Secondary accession number(s): Q920G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 3, 2012
Last modified: April 1, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.