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P57999

- ZAN_RABIT

UniProt

P57999 - ZAN_RABIT

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Protein

Zonadhesin

Gene

ZAN

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling (By similarity).By similarity

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Zonadhesin
Gene namesi
Name:ZAN
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Cell membrane; Single-pass type I membrane protein
Note: Exclusively on the apical region of the sperm head.By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 2282›2282ZonadhesinPRO_0000055637Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi541 – 5411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi569 – 5691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1141 – 11411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1259 – 12591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1270 – 12701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1355 – 13551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1467 – 14671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1483 – 14831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1662 – 16621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1997 – 19971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi2178 – 21781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi2189 ↔ 2200By similarity
Disulfide bondi2194 ↔ 2209By similarity
Disulfide bondi2211 ↔ 2220By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Probably forms covalent oligomers.By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000025091.

Structurei

3D structure databases

ProteinModelPortaliP57999.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini‹1 – 2235›2235ExtracellularSequence AnalysisAdd
BLAST
Topological domaini2257 – 228226CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2236 – 225621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini‹1 – 147›147MAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini150 – 315166MAM 2PROSITE-ProRule annotationAdd
BLAST
Domaini501 – 55050TIL 1Add
BLAST
Domaini551 – 60555VWFC 1Add
BLAST
Domaini611 – 826216VWFD 1PROSITE-ProRule annotationAdd
BLAST
Domaini881 – 93454TIL 2Add
BLAST
Domaini935 – 99056VWFC 2Add
BLAST
Domaini996 – 1213218VWFD 2PROSITE-ProRule annotationAdd
BLAST
Domaini1267 – 132256TIL 3Add
BLAST
Domaini1323 – 137957VWFC 3Add
BLAST
Domaini1385 – 1591207VWFD 3PROSITE-ProRule annotationAdd
BLAST
Domaini1670 – 172657TIL 4Add
BLAST
Domaini1727 – 178256VWFC 4Add
BLAST
Domaini1788 – 1991204VWFD 4PROSITE-ProRule annotationAdd
BLAST
Domaini2076 – 212954TIL 5Add
BLAST
Domaini2130 – 218455VWFC 5Add
BLAST
Domaini2185 – 222137EGF-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni315 – 49818426 X approximate heptapeptide repeats (mucin-like domain)Add
BLAST

Domaini

The MAM domains probably mediate sperm adhesion to the zona pellucida.
During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
The VWFD domains 2 and 3 may mediate covalent oligomerization (By similarity to human intestinal mucin MUC2).

Sequence similaritiesi

Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains at least 2 MAM domains.Curated
Contains 5 VWFC domains.Curated
Contains 4 VWFD domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000168448.
HOVERGENiHBG000375.
InParanoidiP57999.

Family and domain databases

InterProiIPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view]
PfamiPF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTiSM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 3 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF57567. SSF57567. 5 hits.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS50060. MAM_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P57999-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFFATGRASA FSHPCGRTAV SLREERRIKK RKRQDGFYML LDPKNAKPRQ
60 70 80 90 100
KSVLLSPLSQ SAGCLTLSFH YTLWGQSPGA ALSVLASVLG SIRKHTLFSG
110 120 130 140 150
QPSPNWQPVS VNYSGPGQIQ FTVVGVFGDV PEPAVAVDAI SIAPCGESFP
160 170 180 190 200
QCVFEDAAHP FCDWLQASED GGRWAWTDKD MLAQERSLMR ESPHTGHHYI
210 220 230 240 250
YLEADKFSRP GQSVRLVSRP FCAPGDVCVE FSYHMYGLGE GTTLQFLLGS
260 270 280 290 300
PAGSTPVSLW NRVGSQSPDW LNASVTIPSG HQQPMQLVFE AIRGSNTAFV
310 320 330 340 350
VAMSFILINH GTCHVPVPPV IPIKTLVIPT EQPTVPAEGT TEPPEGTIEL
360 370 380 390 400
PEGTTKLPEE TTELPEEITE PPKETTIPTE PPTVPTEPPT VPTEPPTVPT
410 420 430 440 450
EKPTVLTEKP TVPTEETSIP TEPPTVPTEK PTVATEPPTV PTEEPTVATE
460 470 480 490 500
KPTVPTEETT PPTTARSTLT SLEPTTHTPS TSLTSTTLST TTTPSPTTVS
510 520 530 540 550
CPANAHYESC ACPASCKHPK ASCKPPCQPG CVCDPGLVFS NNSCIKASSC
560 570 580 590 600
PCLYNNNNYE PEAEWFSPNC TELCHCWPGG RIECQISQCK THTKCQLKNG
610 620 630 640 650
QYECQPYGTA TCFVYGDPHY VTFDGRHFGF MGKCTYIVAQ PCSKSTDSFF
660 670 680 690 700
RVTAKNEERG QEGMSCLSRV DVTLSETVVT LLKGRRTLVG GQRVTLPAMP
710 720 730 740 750
AKGVFLGPSG RFVELQTDFG LRVRWDGDQQ LLVTVPSAYF QKLCGLCGNY
760 770 780 790 800
DGHSSNDNLK ADGQPAQSEE ELGNSWQWAQ DEDKECQKNQ ANPPSCDTAL
810 820 830 840 850
QTKMSGPQFC GQLVDSRGVF KTCLLHLKAS SFFDNCVFDT CNFQGLQLML
860 870 880 890 900
CAHMSAVTAA CQDAGYAVKP WREPQFCPLA CPPNSRYSLC TSPCPKTCHT
910 920 930 940 950
GYVGMPCPEQ CLEGCECNPG FILSGLECVP SAQCGCLDPS RGYFKVGEQW
960 970 980 990 1000
FKSDCKQLCI CEGSNQIRCQ PWKCGPHEVC SQQSGIYGCH SQGSATCSAS
1010 1020 1030 1040 1050
GDPHYLTFDG ALHHFMGTCN YVLTQPCRHR PQENSFVVSA TNEIRDGNLE
1060 1070 1080 1090 1100
VSLRPSCPRC RSSALKISLV KGHKVVLNGR RVALPVWPSR GQVTVRPSGN
1110 1120 1130 1140 1150
FMLLYTNFGL RVRYDGNHLV EVTVPSSYAG QLCGLCGNYN NNSLDDILGP
1160 1170 1180 1190 1200
YKRPVGNSVQ LGAAWKLEEG SETGCFLQGG KPSSCHEDMG DTWNKNCEVL
1210 1220 1230 1240 1250
VNPLGPFSQC HKVVPPEVSF TSCVHGQCGT KGDSLTLCRS LQAYASLCSL
1260 1270 1280 1290 1300
AGQALAWRNS TFCPLKCPPN SSYSPCGSPC PGTCLSLNHP KDCPITLPCV
1310 1320 1330 1340 1350
EGCECQNGYI LSGTSCVPLN QCGCTDFEGS YHLVRESWYT DNTCSRLCTC
1360 1370 1380 1390 1400
SLHNNITCRQ TACKPGQQCW AVDGLLRCRD SGMGVCQVTG DSRYLSFDGS
1410 1420 1430 1440 1450
SHPLQGACTY VLAKVCHPNM DLPFFKVSAS NEKSSAGGTN AVSLHQVYIE
1460 1470 1480 1490 1500
FSGSLVTLQK GNLVLINGTR VALPATSQIR GLNISSSRTH TIVSFWIGAQ
1510 1520 1530 1540 1550
IKFDGNHVLK ITIPAAYYEK VCGICGNYNG EPEDELMMPS DELAASDLEF
1560 1570 1580 1590 1600
VKSWKDNNID PNCQKSQEGK GKPQEEQGPS GSSKKASCSP ADLQKVQEQC
1610 1620 1630 1640 1650
QAALQTPAWA ECASRVDLRP FLLDCMNSLC EFRGSLQPLC KALQALGAAC
1660 1670 1680 1690 1700
RSKGLQPPIW RNSSFCPLAC PAYSTYTNCL PSCSPSCFDP DGRCEGARAP
1710 1720 1730 1740 1750
SSCAEGCTCQ PGYVLSKNKC VAKDQCSCRD AQGGSIPSGK SWVSSGCSQK
1760 1770 1780 1790 1800
CACTEGSIQC RAFHCPSRSH CKLNSNGNSN CVSEKSDQCS IFGGPHYRTF
1810 1820 1830 1840 1850
DRFSFGFRGR MTYVLIKTVD ELPDGVERLL VQARNKMYPP WNKVFLQEII
1860 1870 1880 1890 1900
TTVYGYKVQL QRDLVLVVNN QKMAVPYKPE DRLRVSMQGQ RLFLITDFEM
1910 1920 1930 1940 1950
VVSFDGRNAA VITLPSMYQG LVRGLCGNYD SDRRNEMMLP NGAITTNVDV
1960 1970 1980 1990 2000
FGNSWEVKTE DSVLRFRRAL QVEADGKEKE TGSYRSECSQ EQLALVNSTQ
2010 2020 2030 2040 2050
ACRVLVDPQG PFAACHQTVA PEPFQEHCVS DLCASRDPKE HEELRCQVLS
2060 2070 2080 2090 2100
GYSITCQEAG IALAGWRDHT HCAMVCPANT VYQSCMTPCP ESCANLAAPR
2110 2120 2130 2140 2150
DCEGPCVEGC ASLPGYAFSG AQSLPLANCG CTSNGIYYQL GHSFVTADCS
2160 2170 2180 2190 2200
QRCTCASSGV LLCEPFSCRP GESCTLGNLT RGCFRESPCL RNPCQNDGRC
2210 2220 2230 2240 2250
REQGTSFTCE CEPGYGGHLC TEPRDVLLPP KPDTSNLVAI LLGMLVSLVV
2260 2270 2280
TVPVLARKCV SRKRRRWREK TQSEPRSAPG RR
Length:2,282
Mass (Da):248,292
Last modified:November 16, 2001 - v2
Checksum:i380FA81093454892
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF244982 mRNA. Translation: AAF63342.2.
RefSeqiNP_001075550.1. NM_001082081.1.
UniGeneiOcu.2652.

Genome annotation databases

GeneIDi100008761.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF244982 mRNA. Translation: AAF63342.2 .
RefSeqi NP_001075550.1. NM_001082081.1.
UniGenei Ocu.2652.

3D structure databases

ProteinModelPortali P57999.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000025091.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100008761.

Organism-specific databases

CTDi 7455.

Phylogenomic databases

eggNOGi NOG12793.
HOGENOMi HOG000168448.
HOVERGENi HBG000375.
InParanoidi P57999.

Family and domain databases

InterProi IPR013320. ConA-like_dom.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view ]
Pfami PF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view ]
SMARTi SM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 3 hits.
SM00216. VWD. 4 hits.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 2 hits.
SSF57567. SSF57567. 5 hits.
PROSITEi PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS50060. MAM_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Zonadhesin: characterization, localization, and zona pellucida binding."
    Lea I.A., Sivashanmugam P., O'Rand M.G.
    Biol. Reprod. 65:1691-1700(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiZAN_RABIT
AccessioniPrimary (citable) accession number: P57999
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 16, 2001
Last modified: October 29, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3