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P57999 (ZAN_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zonadhesin
Gene names
Name:ZAN
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length2282 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling By similarity.

Subunit structure

Probably forms covalent oligomers By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Note: Exclusively on the apical region of the sperm head By similarity.

Domain

The MAM domains probably mediate sperm adhesion to the zona pellucida.

During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.

The VWFD domains 2 and 3 may mediate covalent oligomerization (By similarity to human intestinal mucin MUC2).

Sequence similarities

Contains 1 EGF-like domain.

Contains at least 2 MAM domains.

Contains 5 TIL (trypsin inhibitory-like) domains.

Contains 5 VWFC domains.

Contains 4 VWFD domains.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell membrane
Membrane
   DomainEGF-like domain
Repeat
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 2282›2282Zonadhesin
PRO_0000055637

Regions

Topological domain‹1 – 2235›2235Extracellular Potential
Transmembrane2236 – 225621Helical; Potential
Topological domain2257 – 228226Cytoplasmic Potential
Domain‹1 – 147›147MAM 1
Domain150 – 315166MAM 2
Domain501 – 55050TIL 1
Domain551 – 60555VWFC 1
Domain611 – 826216VWFD 1
Domain881 – 93454TIL 2
Domain935 – 99056VWFC 2
Domain996 – 1213218VWFD 2
Domain1267 – 132256TIL 3
Domain1323 – 137957VWFC 3
Domain1385 – 1591207VWFD 3
Domain1670 – 172657TIL 4
Domain1727 – 178256VWFC 4
Domain1788 – 1991204VWFD 4
Domain2076 – 212954TIL 5
Domain2130 – 218455VWFC 5
Domain2185 – 222137EGF-like
Region315 – 49818426 X approximate heptapeptide repeats (mucin-like domain)

Amino acid modifications

Glycosylation1121N-linked (GlcNAc...) Potential
Glycosylation2721N-linked (GlcNAc...) Potential
Glycosylation5411N-linked (GlcNAc...) Potential
Glycosylation5691N-linked (GlcNAc...) Potential
Glycosylation11411N-linked (GlcNAc...) Potential
Glycosylation12591N-linked (GlcNAc...) Potential
Glycosylation12701N-linked (GlcNAc...) Potential
Glycosylation13551N-linked (GlcNAc...) Potential
Glycosylation14671N-linked (GlcNAc...) Potential
Glycosylation14831N-linked (GlcNAc...) Potential
Glycosylation16621N-linked (GlcNAc...) Potential
Glycosylation19971N-linked (GlcNAc...) Potential
Glycosylation21781N-linked (GlcNAc...) Potential
Disulfide bond2189 ↔ 2200 By similarity
Disulfide bond2194 ↔ 2209 By similarity
Disulfide bond2211 ↔ 2220 By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P57999 [UniParc].

Last modified November 16, 2001. Version 2.
Checksum: 380FA81093454892

FASTA2,282248,292
        10         20         30         40         50         60 
MFFATGRASA FSHPCGRTAV SLREERRIKK RKRQDGFYML LDPKNAKPRQ KSVLLSPLSQ 

        70         80         90        100        110        120 
SAGCLTLSFH YTLWGQSPGA ALSVLASVLG SIRKHTLFSG QPSPNWQPVS VNYSGPGQIQ 

       130        140        150        160        170        180 
FTVVGVFGDV PEPAVAVDAI SIAPCGESFP QCVFEDAAHP FCDWLQASED GGRWAWTDKD 

       190        200        210        220        230        240 
MLAQERSLMR ESPHTGHHYI YLEADKFSRP GQSVRLVSRP FCAPGDVCVE FSYHMYGLGE 

       250        260        270        280        290        300 
GTTLQFLLGS PAGSTPVSLW NRVGSQSPDW LNASVTIPSG HQQPMQLVFE AIRGSNTAFV 

       310        320        330        340        350        360 
VAMSFILINH GTCHVPVPPV IPIKTLVIPT EQPTVPAEGT TEPPEGTIEL PEGTTKLPEE 

       370        380        390        400        410        420 
TTELPEEITE PPKETTIPTE PPTVPTEPPT VPTEPPTVPT EKPTVLTEKP TVPTEETSIP 

       430        440        450        460        470        480 
TEPPTVPTEK PTVATEPPTV PTEEPTVATE KPTVPTEETT PPTTARSTLT SLEPTTHTPS 

       490        500        510        520        530        540 
TSLTSTTLST TTTPSPTTVS CPANAHYESC ACPASCKHPK ASCKPPCQPG CVCDPGLVFS 

       550        560        570        580        590        600 
NNSCIKASSC PCLYNNNNYE PEAEWFSPNC TELCHCWPGG RIECQISQCK THTKCQLKNG 

       610        620        630        640        650        660 
QYECQPYGTA TCFVYGDPHY VTFDGRHFGF MGKCTYIVAQ PCSKSTDSFF RVTAKNEERG 

       670        680        690        700        710        720 
QEGMSCLSRV DVTLSETVVT LLKGRRTLVG GQRVTLPAMP AKGVFLGPSG RFVELQTDFG 

       730        740        750        760        770        780 
LRVRWDGDQQ LLVTVPSAYF QKLCGLCGNY DGHSSNDNLK ADGQPAQSEE ELGNSWQWAQ 

       790        800        810        820        830        840 
DEDKECQKNQ ANPPSCDTAL QTKMSGPQFC GQLVDSRGVF KTCLLHLKAS SFFDNCVFDT 

       850        860        870        880        890        900 
CNFQGLQLML CAHMSAVTAA CQDAGYAVKP WREPQFCPLA CPPNSRYSLC TSPCPKTCHT 

       910        920        930        940        950        960 
GYVGMPCPEQ CLEGCECNPG FILSGLECVP SAQCGCLDPS RGYFKVGEQW FKSDCKQLCI 

       970        980        990       1000       1010       1020 
CEGSNQIRCQ PWKCGPHEVC SQQSGIYGCH SQGSATCSAS GDPHYLTFDG ALHHFMGTCN 

      1030       1040       1050       1060       1070       1080 
YVLTQPCRHR PQENSFVVSA TNEIRDGNLE VSLRPSCPRC RSSALKISLV KGHKVVLNGR 

      1090       1100       1110       1120       1130       1140 
RVALPVWPSR GQVTVRPSGN FMLLYTNFGL RVRYDGNHLV EVTVPSSYAG QLCGLCGNYN 

      1150       1160       1170       1180       1190       1200 
NNSLDDILGP YKRPVGNSVQ LGAAWKLEEG SETGCFLQGG KPSSCHEDMG DTWNKNCEVL 

      1210       1220       1230       1240       1250       1260 
VNPLGPFSQC HKVVPPEVSF TSCVHGQCGT KGDSLTLCRS LQAYASLCSL AGQALAWRNS 

      1270       1280       1290       1300       1310       1320 
TFCPLKCPPN SSYSPCGSPC PGTCLSLNHP KDCPITLPCV EGCECQNGYI LSGTSCVPLN 

      1330       1340       1350       1360       1370       1380 
QCGCTDFEGS YHLVRESWYT DNTCSRLCTC SLHNNITCRQ TACKPGQQCW AVDGLLRCRD 

      1390       1400       1410       1420       1430       1440 
SGMGVCQVTG DSRYLSFDGS SHPLQGACTY VLAKVCHPNM DLPFFKVSAS NEKSSAGGTN 

      1450       1460       1470       1480       1490       1500 
AVSLHQVYIE FSGSLVTLQK GNLVLINGTR VALPATSQIR GLNISSSRTH TIVSFWIGAQ 

      1510       1520       1530       1540       1550       1560 
IKFDGNHVLK ITIPAAYYEK VCGICGNYNG EPEDELMMPS DELAASDLEF VKSWKDNNID 

      1570       1580       1590       1600       1610       1620 
PNCQKSQEGK GKPQEEQGPS GSSKKASCSP ADLQKVQEQC QAALQTPAWA ECASRVDLRP 

      1630       1640       1650       1660       1670       1680 
FLLDCMNSLC EFRGSLQPLC KALQALGAAC RSKGLQPPIW RNSSFCPLAC PAYSTYTNCL 

      1690       1700       1710       1720       1730       1740 
PSCSPSCFDP DGRCEGARAP SSCAEGCTCQ PGYVLSKNKC VAKDQCSCRD AQGGSIPSGK 

      1750       1760       1770       1780       1790       1800 
SWVSSGCSQK CACTEGSIQC RAFHCPSRSH CKLNSNGNSN CVSEKSDQCS IFGGPHYRTF 

      1810       1820       1830       1840       1850       1860 
DRFSFGFRGR MTYVLIKTVD ELPDGVERLL VQARNKMYPP WNKVFLQEII TTVYGYKVQL 

      1870       1880       1890       1900       1910       1920 
QRDLVLVVNN QKMAVPYKPE DRLRVSMQGQ RLFLITDFEM VVSFDGRNAA VITLPSMYQG 

      1930       1940       1950       1960       1970       1980 
LVRGLCGNYD SDRRNEMMLP NGAITTNVDV FGNSWEVKTE DSVLRFRRAL QVEADGKEKE 

      1990       2000       2010       2020       2030       2040 
TGSYRSECSQ EQLALVNSTQ ACRVLVDPQG PFAACHQTVA PEPFQEHCVS DLCASRDPKE 

      2050       2060       2070       2080       2090       2100 
HEELRCQVLS GYSITCQEAG IALAGWRDHT HCAMVCPANT VYQSCMTPCP ESCANLAAPR 

      2110       2120       2130       2140       2150       2160 
DCEGPCVEGC ASLPGYAFSG AQSLPLANCG CTSNGIYYQL GHSFVTADCS QRCTCASSGV 

      2170       2180       2190       2200       2210       2220 
LLCEPFSCRP GESCTLGNLT RGCFRESPCL RNPCQNDGRC REQGTSFTCE CEPGYGGHLC 

      2230       2240       2250       2260       2270       2280 
TEPRDVLLPP KPDTSNLVAI LLGMLVSLVV TVPVLARKCV SRKRRRWREK TQSEPRSAPG 


RR

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References

[1]"Zonadhesin: characterization, localization, and zona pellucida binding."
Lea I.A., Sivashanmugam P., O'Rand M.G.
Biol. Reprod. 65:1691-1700(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF244982 mRNA. Translation: AAF63342.2.
RefSeqNP_001075550.1. NM_001082081.1.
UniGeneOcu.2652.

3D structure databases

ProteinModelPortalP57999.
ModBaseSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000025091.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100008761.

Organism-specific databases

CTD7455.

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000168448.
HOVERGENHBG000375.
OrthoDBEOG4BZN1V.

Family and domain databases

InterProIPR008985. ConA-like_lec_gl_sf.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR000998. MAM_dom.
IPR002919. TIL_dom.
IPR025615. TILa_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view]
PfamPF08742. C8. 4 hits.
PF00008. EGF. 1 hit.
PF00629. MAM. 2 hits.
PF01826. TIL. 5 hits.
PF12714. TILa. 5 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTSM00832. C8. 4 hits.
SM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00214. VWC. 3 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMSSF49899. ConA_like_lec_gl. 2 hits.
SSF57567. Cysrich_TIL. 5 hits.
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. False negative.
PS50060. MAM_2. 2 hits.
PS01208. VWFC_1. False negative.
PS50184. VWFC_2. False negative.
PS51233. VWFD. 4 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameZAN_RABIT
AccessionPrimary (citable) accession number: P57999
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 16, 2001
Last modified: April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents