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P57999

- ZAN_RABIT

UniProt

P57999 - ZAN_RABIT

Protein

Zonadhesin

Gene

ZAN

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (16 Nov 2001)
      Previous versions | rss
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    Functioni

    Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling By similarity.By similarity

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zonadhesin
    Gene namesi
    Name:ZAN
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein
    Note: Exclusively on the apical region of the sperm head.By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 2282›2282ZonadhesinPRO_0000055637Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi541 – 5411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi569 – 5691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1141 – 11411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1259 – 12591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1270 – 12701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1355 – 13551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1467 – 14671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1483 – 14831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1662 – 16621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1997 – 19971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi2178 – 21781N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi2189 ↔ 2200By similarity
    Disulfide bondi2194 ↔ 2209By similarity
    Disulfide bondi2211 ↔ 2220By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Probably forms covalent oligomers.By similarity

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000025091.

    Structurei

    3D structure databases

    ProteinModelPortaliP57999.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini‹1 – 2235›2235ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini2257 – 228226CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2236 – 225621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini‹1 – 147›147MAM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini150 – 315166MAM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini501 – 55050TIL 1Add
    BLAST
    Domaini551 – 60555VWFC 1Add
    BLAST
    Domaini611 – 826216VWFD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini881 – 93454TIL 2Add
    BLAST
    Domaini935 – 99056VWFC 2Add
    BLAST
    Domaini996 – 1213218VWFD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1267 – 132256TIL 3Add
    BLAST
    Domaini1323 – 137957VWFC 3Add
    BLAST
    Domaini1385 – 1591207VWFD 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1670 – 172657TIL 4Add
    BLAST
    Domaini1727 – 178256VWFC 4Add
    BLAST
    Domaini1788 – 1991204VWFD 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini2076 – 212954TIL 5Add
    BLAST
    Domaini2130 – 218455VWFC 5Add
    BLAST
    Domaini2185 – 222137EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni315 – 49818426 X approximate heptapeptide repeats (mucin-like domain)Add
    BLAST

    Domaini

    The MAM domains probably mediate sperm adhesion to the zona pellucida.
    During sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus.
    The VWFD domains 2 and 3 may mediate covalent oligomerization (By similarity to human intestinal mucin MUC2).

    Sequence similaritiesi

    Contains 1 EGF-like domain.PROSITE-ProRule annotation
    Contains at least 2 MAM domains.Curated
    Contains 5 VWFC domains.Curated
    Contains 4 VWFD domains.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000168448.
    HOVERGENiHBG000375.

    Family and domain databases

    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000998. MAM_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    [Graphical view]
    PfamiPF08742. C8. 4 hits.
    PF00008. EGF. 1 hit.
    PF00629. MAM. 2 hits.
    PF01826. TIL. 5 hits.
    PF12714. TILa. 5 hits.
    PF00094. VWD. 4 hits.
    [Graphical view]
    SMARTiSM00832. C8. 4 hits.
    SM00181. EGF. 1 hit.
    SM00137. MAM. 1 hit.
    SM00214. VWC. 3 hits.
    SM00216. VWD. 4 hits.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 2 hits.
    SSF57567. SSF57567. 5 hits.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 1 hit.
    PS50060. MAM_2. 2 hits.
    PS51233. VWFD. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P57999-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFFATGRASA FSHPCGRTAV SLREERRIKK RKRQDGFYML LDPKNAKPRQ     50
    KSVLLSPLSQ SAGCLTLSFH YTLWGQSPGA ALSVLASVLG SIRKHTLFSG 100
    QPSPNWQPVS VNYSGPGQIQ FTVVGVFGDV PEPAVAVDAI SIAPCGESFP 150
    QCVFEDAAHP FCDWLQASED GGRWAWTDKD MLAQERSLMR ESPHTGHHYI 200
    YLEADKFSRP GQSVRLVSRP FCAPGDVCVE FSYHMYGLGE GTTLQFLLGS 250
    PAGSTPVSLW NRVGSQSPDW LNASVTIPSG HQQPMQLVFE AIRGSNTAFV 300
    VAMSFILINH GTCHVPVPPV IPIKTLVIPT EQPTVPAEGT TEPPEGTIEL 350
    PEGTTKLPEE TTELPEEITE PPKETTIPTE PPTVPTEPPT VPTEPPTVPT 400
    EKPTVLTEKP TVPTEETSIP TEPPTVPTEK PTVATEPPTV PTEEPTVATE 450
    KPTVPTEETT PPTTARSTLT SLEPTTHTPS TSLTSTTLST TTTPSPTTVS 500
    CPANAHYESC ACPASCKHPK ASCKPPCQPG CVCDPGLVFS NNSCIKASSC 550
    PCLYNNNNYE PEAEWFSPNC TELCHCWPGG RIECQISQCK THTKCQLKNG 600
    QYECQPYGTA TCFVYGDPHY VTFDGRHFGF MGKCTYIVAQ PCSKSTDSFF 650
    RVTAKNEERG QEGMSCLSRV DVTLSETVVT LLKGRRTLVG GQRVTLPAMP 700
    AKGVFLGPSG RFVELQTDFG LRVRWDGDQQ LLVTVPSAYF QKLCGLCGNY 750
    DGHSSNDNLK ADGQPAQSEE ELGNSWQWAQ DEDKECQKNQ ANPPSCDTAL 800
    QTKMSGPQFC GQLVDSRGVF KTCLLHLKAS SFFDNCVFDT CNFQGLQLML 850
    CAHMSAVTAA CQDAGYAVKP WREPQFCPLA CPPNSRYSLC TSPCPKTCHT 900
    GYVGMPCPEQ CLEGCECNPG FILSGLECVP SAQCGCLDPS RGYFKVGEQW 950
    FKSDCKQLCI CEGSNQIRCQ PWKCGPHEVC SQQSGIYGCH SQGSATCSAS 1000
    GDPHYLTFDG ALHHFMGTCN YVLTQPCRHR PQENSFVVSA TNEIRDGNLE 1050
    VSLRPSCPRC RSSALKISLV KGHKVVLNGR RVALPVWPSR GQVTVRPSGN 1100
    FMLLYTNFGL RVRYDGNHLV EVTVPSSYAG QLCGLCGNYN NNSLDDILGP 1150
    YKRPVGNSVQ LGAAWKLEEG SETGCFLQGG KPSSCHEDMG DTWNKNCEVL 1200
    VNPLGPFSQC HKVVPPEVSF TSCVHGQCGT KGDSLTLCRS LQAYASLCSL 1250
    AGQALAWRNS TFCPLKCPPN SSYSPCGSPC PGTCLSLNHP KDCPITLPCV 1300
    EGCECQNGYI LSGTSCVPLN QCGCTDFEGS YHLVRESWYT DNTCSRLCTC 1350
    SLHNNITCRQ TACKPGQQCW AVDGLLRCRD SGMGVCQVTG DSRYLSFDGS 1400
    SHPLQGACTY VLAKVCHPNM DLPFFKVSAS NEKSSAGGTN AVSLHQVYIE 1450
    FSGSLVTLQK GNLVLINGTR VALPATSQIR GLNISSSRTH TIVSFWIGAQ 1500
    IKFDGNHVLK ITIPAAYYEK VCGICGNYNG EPEDELMMPS DELAASDLEF 1550
    VKSWKDNNID PNCQKSQEGK GKPQEEQGPS GSSKKASCSP ADLQKVQEQC 1600
    QAALQTPAWA ECASRVDLRP FLLDCMNSLC EFRGSLQPLC KALQALGAAC 1650
    RSKGLQPPIW RNSSFCPLAC PAYSTYTNCL PSCSPSCFDP DGRCEGARAP 1700
    SSCAEGCTCQ PGYVLSKNKC VAKDQCSCRD AQGGSIPSGK SWVSSGCSQK 1750
    CACTEGSIQC RAFHCPSRSH CKLNSNGNSN CVSEKSDQCS IFGGPHYRTF 1800
    DRFSFGFRGR MTYVLIKTVD ELPDGVERLL VQARNKMYPP WNKVFLQEII 1850
    TTVYGYKVQL QRDLVLVVNN QKMAVPYKPE DRLRVSMQGQ RLFLITDFEM 1900
    VVSFDGRNAA VITLPSMYQG LVRGLCGNYD SDRRNEMMLP NGAITTNVDV 1950
    FGNSWEVKTE DSVLRFRRAL QVEADGKEKE TGSYRSECSQ EQLALVNSTQ 2000
    ACRVLVDPQG PFAACHQTVA PEPFQEHCVS DLCASRDPKE HEELRCQVLS 2050
    GYSITCQEAG IALAGWRDHT HCAMVCPANT VYQSCMTPCP ESCANLAAPR 2100
    DCEGPCVEGC ASLPGYAFSG AQSLPLANCG CTSNGIYYQL GHSFVTADCS 2150
    QRCTCASSGV LLCEPFSCRP GESCTLGNLT RGCFRESPCL RNPCQNDGRC 2200
    REQGTSFTCE CEPGYGGHLC TEPRDVLLPP KPDTSNLVAI LLGMLVSLVV 2250
    TVPVLARKCV SRKRRRWREK TQSEPRSAPG RR 2282
    Length:2,282
    Mass (Da):248,292
    Last modified:November 16, 2001 - v2
    Checksum:i380FA81093454892
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF244982 mRNA. Translation: AAF63342.2.
    RefSeqiNP_001075550.1. NM_001082081.1.
    UniGeneiOcu.2652.

    Genome annotation databases

    GeneIDi100008761.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF244982 mRNA. Translation: AAF63342.2 .
    RefSeqi NP_001075550.1. NM_001082081.1.
    UniGenei Ocu.2652.

    3D structure databases

    ProteinModelPortali P57999.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000025091.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100008761.

    Organism-specific databases

    CTDi 7455.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000168448.
    HOVERGENi HBG000375.

    Family and domain databases

    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR000998. MAM_dom.
    IPR002919. TIL_dom.
    IPR025615. TILa_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    [Graphical view ]
    Pfami PF08742. C8. 4 hits.
    PF00008. EGF. 1 hit.
    PF00629. MAM. 2 hits.
    PF01826. TIL. 5 hits.
    PF12714. TILa. 5 hits.
    PF00094. VWD. 4 hits.
    [Graphical view ]
    SMARTi SM00832. C8. 4 hits.
    SM00181. EGF. 1 hit.
    SM00137. MAM. 1 hit.
    SM00214. VWC. 3 hits.
    SM00216. VWD. 4 hits.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 2 hits.
    SSF57567. SSF57567. 5 hits.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 4 hits.
    PS50026. EGF_3. 1 hit.
    PS50060. MAM_2. 2 hits.
    PS51233. VWFD. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Zonadhesin: characterization, localization, and zona pellucida binding."
      Lea I.A., Sivashanmugam P., O'Rand M.G.
      Biol. Reprod. 65:1691-1700(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiZAN_RABIT
    AccessioniPrimary (citable) accession number: P57999
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: November 16, 2001
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3