P57994 (MURC_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	ML0915

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182118
Regions
	Nucleotide binding	122 – 128	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P57994 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 46F816A650C847A7	FASTA	495	51,588
	10 20 30 40 50 60 MNAGQLPPEL QRVHMVGIGG SGMSGIARIL LDRGGLVSGS DAKESRVVHA LRARGALIRI 70 80 90 100 110 120 GHDASLLDLL PGGATAVITI RTAIPKTNPE LVEARRRGIP VLLRSAVLAR LMDGCTTLMV 130 140 150 160 170 180 AGTHGKTTTT SMLVVALQHC GCDPSFVVGG ELAVVGTNAH HGLGACFVAE ADESDGSLLE 190 200 210 220 230 240 YTPNVAVVTN IDSDHLDFYG SVDAYIRVFD SFVERFALGG ALVVCNDDPG AAALARRTAE 250 260 270 280 290 300 LGIRVLRYGS DDRIGETLAA RLLSWEQQGT GAVAHIQLAG QPNSRVMRLP VPGRHMALNA 310 320 330 340 350 360 LGALLAAIEI GASTDEVLDG LAGFRGVRRR FELVGTSGVG QASNSLVRVF DDYAHHPTEI 370 380 390 400 410 420 SATLAAFRIM LEQSGGGRCI VVFQPHLYSR TKALSREFGR ALSAADEVFI VGVNGAREQP 430 440 450 460 470 480 LAGVSGASVA KHVSVPVRYI PDYSAVVIEV AAAAGPGDVI VTMGIGDVGL LGPEIVAALR 490 VRANCNTPGC SGVLQ

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL583920 Genomic DNA. Translation: CAC31296.1.
PIR	E87023.
RefSeq	NP_301698.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P57994.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000028460; EBMYCP00000028066; EBMYCG00000028455.
GeneID	909916.
GenomeReviews	Gene locus ML0915 in contig AL450380_GR.
KEGG	mle:ML0915.
NMPDR	fig|272631.1.peg.570.
PATRIC	18053426. VBIMycLep78757_1658.
Organism-specific databases
Leproma	ML0915.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015779.
HOGENOM	HBG476594.
OMA	KAGTNAH.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0915-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_MYCLE

Accession

Primary (citable) accession number: P57994

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program