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P57978

- LIPA_PASMU

UniProt

P57978 - LIPA_PASMU

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Protein
Lipoyl synthase
Gene
lipA, PM1930
Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi78 – 781Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi93 – 931Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-2005-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:PM1930
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Lipoyl synthaseUniRule annotation
PRO_0000102332Add
BLAST

Proteomic databases

PRIDEiP57978.

Interactioni

Protein-protein interaction databases

STRINGi272843.PM1930.

Structurei

3D structure databases

ProteinModelPortaliP57978.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

P57978-1 [UniParc]FASTAAdd to Basket

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MGTPFKMERG VKYRDAAKTS IIPVKNIDPN QELLKKPEWM KIKLPANSAK    50
IDSIKNGMRR HGLHSVCEEA SCPNLHECFN HGTATFMILG AICTRRCPFC 100
DVAHGKPLPP DPEEPRKLAE TIQDMKLRYV VITSVDRDDL PDRGAGHFAE 150
CVKEIRQLNP NIKIEILVPD FRGRIEQALD KLKDNPPDVF NHNLENVPRL 200
YREIRPGADY QWSLKLLKDF KAMFPHIPTK SGLMVGLGET NEEILQVMQD 250
LRDHGVTMLT LGQYLQPSRH HLPVARYVPP AEFDEFRDKA QAMGFEHAAC 300
GPFVRSSYHA DLQAKGEIVK 320
Length:320
Mass (Da):36,349
Last modified:April 27, 2001 - v1
Checksum:i9CE70DAC1905482F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK04014.1.
RefSeqiNP_246869.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK04014; AAK04014; PM1930.
GeneIDi1245277.
KEGGipmu:PM1930.
PATRICi22873183. VBIPasMul88067_1952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004439 Genomic DNA. Translation: AAK04014.1 .
RefSeqi NP_246869.1. NC_002663.1.

3D structure databases

ProteinModelPortali P57978.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272843.PM1930.

Proteomic databases

PRIDEi P57978.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK04014 ; AAK04014 ; PM1930 .
GeneIDi 1245277.
KEGGi pmu:PM1930.
PATRICi 22873183. VBIPasMul88067_1952.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci PMUL272843:GC8W-2005-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiLIPA_PASMU
AccessioniPrimary (citable) accession number: P57978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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