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P57877 (ASSY_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Argininosuccinate synthase
EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:PM0813
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella ›

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00581

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00581

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 445445Argininosuccinate synthase HAMAP-Rule MF_00581
PRO_0000148703

Regions

Nucleotide binding18 – 269ATP By similarity

Sites

Binding site441ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site1001Citrulline By similarity
Binding site1301ATP; via amide nitrogen By similarity
Binding site1321Aspartate By similarity
Binding site1321ATP By similarity
Binding site1361Aspartate By similarity
Binding site1361Citrulline By similarity
Binding site1371Aspartate By similarity
Binding site1371ATP By similarity
Binding site1401Citrulline By similarity
Binding site1931Citrulline By similarity
Binding site1951ATP By similarity
Binding site2021Citrulline By similarity
Binding site2041Citrulline By similarity
Binding site2811Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
P57877 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: F00D97C3C2C42E0A

FASTA44549,993
        10         20         30         40         50         60 
MSNTILQQLP KGQKVGIAFS GGLDTSAALL WMRQKGAVPY AYTANLGQPD EEDYNAIPRK 

        70         80         90        100        110        120 
AMEYGAENAR LIDCRAQLAH EGIAAIQSGA FHISTGGVTY FNTTPLGRAV TGTMLVSAMR 

       130        140        150        160        170        180 
EDDVNIWGDG STFKGNDIER FYRYGLLTNP NLKIYKPWLD NQFIEELGGR HEMSEFLIAN 

       190        200        210        220        230        240 
GFEYKMSVEK AYSTDSNMLG ATHEAKDLEF LNTGIRIVKP IMGVAFWRDD VEVKAEEVTV 

       250        260        270        280        290        300 
RFEEGIPVAL NGKTFSDHVE LFLEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGMAL 

       310        320        330        340        350        360 
LHIAYERLVS GIHNEDTIEQ YRINGLRLGR LLYQGRWFDP QALMLRETAQ RWVARAITGE 

       370        380        390        400        410        420 
VTFELRRGND YSILNTESPN LTYMPERLSM EKVENAPFDP VDRIGQLTMR NLDIVDTRDK 

       430        440 
LGIYTQTGLL SISKDSFLPQ LNKKG

« Hide

References

[1]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004439 Genomic DNA. Translation: AAK02897.1.
RefSeqNP_245750.1. NC_002663.1.

3D structure databases

ProteinModelPortalP57877.
SMRP57877. Positions 4-441.
ModBaseSearch...

Protein-protein interaction databases

STRING272843.PM0813.

Proteomic databases

PRIDEP57877.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK02897; AAK02897; PM0813.
GeneID1244160.
KEGGpmu:PM0813.
PATRIC22870824. VBIPasMul88067_0822.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230094.
KOK01940.
OMAGGRKEMS.
ProtClustDBPRK05370.

Enzyme and pathway databases

UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00581. Arg_succ_synth_type2.
InterProIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR11587. PTHR11587. 1 hit.
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_PASMU
AccessionPrimary (citable) accession number: P57877
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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