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Protein

Argininosuccinate synthase

Gene

argG

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei100 – 1001CitrullineBy similarity
Binding sitei130 – 1301ATP; via amide nitrogenBy similarity
Binding sitei132 – 1321AspartateBy similarity
Binding sitei132 – 1321ATPBy similarity
Binding sitei136 – 1361AspartateBy similarity
Binding sitei136 – 1361CitrullineBy similarity
Binding sitei137 – 1371AspartateBy similarity
Binding sitei137 – 1371ATPBy similarity
Binding sitei140 – 1401CitrullineBy similarity
Binding sitei193 – 1931CitrullineBy similarity
Binding sitei195 – 1951ATPBy similarity
Binding sitei202 – 2021CitrullineBy similarity
Binding sitei204 – 2041CitrullineBy similarity
Binding sitei281 – 2811CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMUL272843:GC8W-839-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:PM0813
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
ProteomesiUP000000809 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Argininosuccinate synthasePRO_0000148703Add
BLAST

Proteomic databases

PRIDEiP57877.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272843.PM0813.

Structurei

3D structure databases

ProteinModelPortaliP57877.
SMRiP57877. Positions 4-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTILQQLP KGQKVGIAFS GGLDTSAALL WMRQKGAVPY AYTANLGQPD
60 70 80 90 100
EEDYNAIPRK AMEYGAENAR LIDCRAQLAH EGIAAIQSGA FHISTGGVTY
110 120 130 140 150
FNTTPLGRAV TGTMLVSAMR EDDVNIWGDG STFKGNDIER FYRYGLLTNP
160 170 180 190 200
NLKIYKPWLD NQFIEELGGR HEMSEFLIAN GFEYKMSVEK AYSTDSNMLG
210 220 230 240 250
ATHEAKDLEF LNTGIRIVKP IMGVAFWRDD VEVKAEEVTV RFEEGIPVAL
260 270 280 290 300
NGKTFSDHVE LFLEANRIGG RHGLGMSDQI ENRIIEAKSR GIYEAPGMAL
310 320 330 340 350
LHIAYERLVS GIHNEDTIEQ YRINGLRLGR LLYQGRWFDP QALMLRETAQ
360 370 380 390 400
RWVARAITGE VTFELRRGND YSILNTESPN LTYMPERLSM EKVENAPFDP
410 420 430 440
VDRIGQLTMR NLDIVDTRDK LGIYTQTGLL SISKDSFLPQ LNKKG
Length:445
Mass (Da):49,993
Last modified:April 27, 2001 - v1
Checksum:iF00D97C3C2C42E0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK02897.1.
RefSeqiWP_005722481.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK02897; AAK02897; PM0813.
GeneIDi1244160.
KEGGipmu:PM0813.
PATRICi22870824. VBIPasMul88067_0822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK02897.1.
RefSeqiWP_005722481.1. NC_002663.1.

3D structure databases

ProteinModelPortaliP57877.
SMRiP57877. Positions 4-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272843.PM0813.

Proteomic databases

PRIDEiP57877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK02897; AAK02897; PM0813.
GeneIDi1244160.
KEGGipmu:PM0813.
PATRICi22870824. VBIPasMul88067_0822.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciPMUL272843:GC8W-839-MONOMER.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pm70.

Entry informationi

Entry nameiASSY_PASMU
AccessioniPrimary (citable) accession number: P57877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: July 22, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.