Reviewed, UniProtKB/Swiss-Prot P57863 (RIBA_PASMU)

Names and origin

Protein names

Recommended name:
    GTP cyclohydrolase-2
    EC=3.5.4.25
Alternative name(s):
    GTP cyclohydrolase II

Gene names

Name:	ribA
Ordered Locus Names:	PM0677

Organism

Pasteurella multocida [Complete proteome] [HAMAP]

Taxonomic identifier

747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.
Catalytic activity	GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_00179
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 6,7-dimethyl-8-(1-D-ribityl)lumazine from GTP: step 1/4. HAMAP MF_00179
Sequence similarities	Belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	GTP cyclohydrolase-2 HAMAP MF_00179		PRO_0000151766
Regions
	Nucleotide binding	51 – 55	5	GTP By similarity
	Nucleotide binding	94 – 96	3	GTP By similarity
Sites
	Active site	128	1	Proton acceptor Potential
	Active site	130	1	Nucleophile By similarity
	Metal binding	56	1	Zinc; catalytic By similarity
	Metal binding	67	1	Zinc; catalytic By similarity
	Metal binding	69	1	Zinc; catalytic By similarity
	Binding site	72	1	GTP By similarity
	Binding site	116	1	GTP By similarity
	Binding site	151	1	GTP By similarity
	Binding site	156	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57863-1 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: EA76FC6FF716BF55	FASTA	219	24,634
	10 20 30 40 50 60 MAKIQRVTEA NLPTEFGMFR IVGFEFPDTK KEHVALVLGE VENTDEPILA RIHSECLTGD 70 80 90 100 110 120 ALYSLKCDCG FQLAAALRQI SQEGRGVLIY HREEGRGIGL INKIRAYSLQ DKGMDTIEAN 130 140 150 160 170 180 LALGFAADER NFEVCADIFA LLGINKVRLL TNNPNKIDTM KKAGINIVER VALNVGENRY 190 200 210 NTEYLDTKAK KMGHFIIHNQ QKYPLECPYC SEEVPVQEK

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004439 Genomic DNA. Translation: AAK02761.1.
RefSeq	NP_245614.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1244024.
GenomeReviews	Gene locus PM0677 in contig AE004439_GR.
KEGG	pmu:PM0677.
NMPDR	fig|272843.1.peg.677.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57863.
OMA	P57863. QGFILYL.
Enzyme and pathway databases
BRENDA	3.5.4.25. 258935.
Family and domain databases
HAMAP	MF_00179. [Tree]
InterPro	IPR000926. GTP_CycHdrlase_II. [Graphical view]
Pfam	PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00505. ribA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBA_PASMU

Accession

Primary (citable) accession number: P57863

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)