Phenylalanine--tRNA ligase beta chain - Pasteurella multocida (strain Pm70)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	PM0629

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	795 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00283
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00283.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 795

795

Phenylalanine--tRNA ligase beta chain HAMAP MF_00283

PRO_0000126924

Regions

Domain

39 – 148

110

tRNA-binding

Domain

401 – 476

76

B5

Domain

701 – 794

94

FDX-ACB

Sites

Metal binding

454

1

Magnesium By similarity

Metal binding

460

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

463

1

Magnesium By similarity

Metal binding

464

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P57859 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: 7A7AD664F7202D68
Show »

FASTA

795

86,926

        10         20         30         40         50         60 
MKFSESWVRE WVNPAISTAQ LCDQITMLGL EVDGVEKVAG DFSGVVVGEV VECAQHPDAD 

        70         80         90        100        110        120 
KLRVTKVNVG GDRLLDIVCG APNCRQGLKV ACATEGAVLP GDFKIKKTKL RGQPSEGMLC 

       130        140        150        160        170        180 
SFSELGIDVE SSGIIELPQN APVGTNLRDY LTLDDNTVEI SLTPNRADCL SIAGIAREIG 

       190        200        210        220        230        240 
VVNKLAVNTP HFESVKATIT DKVDIQLHAP EACPRYLLRV VKNVNVQAPS PMWLQEKLRR 

       250        260        270        280        290        300 
CGIRSIDPVV DVTNYILLEL GQPMHAFDAA KVAQPVQVRM AKSGEALVLL DGSTAKLKDN 

       310        320        330        340        350        360 
TLLIADQNGP LAMAGIFGGQ ASGVNAETKD IILEAAFFAP LAIAGRARQY GLHTDSSHRF 

       370        380        390        400        410        420 
ERGVDFELQR QAMERATALL VEICGGEVGD ICEVASDAFL PKLNQVSLRR EKLDALLGHH 

       430        440        450        460        470        480 
IETETVTDIL TRLGLQVSYA NDVWQVTSAS WRFDIEIEED LVEEIARIYG YNSIPNKAPL 

       490        500        510        520        530        540 
AHLRMREHKE ADLDLARIKT ALVDADYQEA ITYSFVDPKI QSVLHPQQDA LVLPNPISVE 

       550        560        570        580        590        600 
MSAMRLSLLS GLLGAVQYNQ NRQQTRVRLF ETGLRFIPDT QAEFGVRQEF VLAAVMTGNK 

       610        620        630        640        650        660 
KAEHWAGKAE SVDFFDLKGD LESILSLTGT RNLVKFVAKS YPALHPGQSA AIMLNGEEIG 

       670        680        690        700        710        720 
FIGTLHPNAA KQLGLNGKTV VFEILWQAIA TRQVVQAKEI SRFPANRRDL AIVVAESVAA 

       730        740        750        760        770        780 
SDVLDACREV AGETLTQVNL FDVYQGNGVA EGHKSLAISL IIQDNEKTLE EDDINAVVSV 

       790 
VLSELKQRFN AYLRD

« Hide

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK02713.1.
RefSeq	NP_245566.1. NC_002663.1.
3D structure databases
ProteinModelPortal	P57859.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1243976.
GenomeReviews	Gene locus PM0629 in contig AE004439_GR.
KEGG	pmu:PM0629.
NMPDR	fig\|272843.1.peg.629.
PATRIC	22870442. VBIPasMul88067_0637.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG399638.
OMA	ADKLRVC.
ProtClustDB	PRK00629.
Enzyme and pathway databases
BioCyc	PMUL272843:PM0629-MONOMER.
Family and domain databases
HAMAP	MF_00283. Phe_tRNA_synth_beta1. [Tree]
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004532. Phe-tRNA-synth_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Gene3D	G3DSA:3.50.40.10. B3_4. 1 hit. G3DSA:3.30.56.20. B5. 1 hit. G3DSA:3.30.70.380. Fdx_AntiC_bd. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01890.
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. PheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_PASMU

Accession

Primary (citable) accession number: P57859

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents