Reviewed, UniProtKB/Swiss-Prot P57847 (SYQ_PASMU)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	PM0528

Organism

Pasteurella multocida [Complete proteome] [HAMAP]

Taxonomic identifier

747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_0000195841
Regions
	Motif	44 – 54	11	"HIGH" region HAMAP MF_00126
	Motif	279 – 283	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	282	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57847-1 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: C3DBC90B65F071D1	FASTA	559	64,310
	10 20 30 40 50 60 MSHPVENVVA AENTEKRPTN FIRQIIDEDL ASGKHTGVQT RFPPEPNGYL HIGHAKSICL 70 80 90 100 110 120 NFGIAEDYQG LCNLRFDDTN PVKEDVEYVD SIKQDVEWLG FKWEGEPRYA SDYFDQLYGY 130 140 150 160 170 180 AIELIEKGLA YVDELSPEEM REYRGTLTEP GKNSPYRDRS IEENLALFEK MKNGEIAEGK 190 200 210 220 230 240 ACLRAKIDMA SPFIVMRDPV IYRIKFATHH QTGDKWCIYP MYDFTHCISD AIERITHSLC 250 260 270 280 290 300 TLEFQDNRRL YDWVLENISI ARPLPHQYEF SRLNLESTLT SKRKLLQLVN EGIVDGWNDP 310 320 330 340 350 360 RMPTISGLRR RGYTPASLRE FCRRIGVTKQ DNMVEFSALE ACIREDLNEN APRAMAVINP 370 380 390 400 410 420 LKIVIENFSG KEMLTAPNHP NRDELGVREL PFTRELYIDQ ADFREEANKQ YKRLVLGKEV 430 440 450 460 470 480 RLRNAYVIKA ERVEKDAEEN ITTVYCTYDP DTLGKNPADG RKVKGVIQWV SAEDYLPAEF 490 500 510 520 530 540 RQYGRLFTVA NPGAAEDIHQ VLNPDSLVIK QGVVEKSLAN AQPEKAYQFE REGYYCADSK 550 DSRPDHLVFN LTVSLKEGF

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004439 Genomic DNA. Translation: AAK02612.1.
RefSeq	NP_245465.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYL based on UniProtKB P00962.
SMR	P57847. Positions 19-557.
ModBase	Search...
Proteomic databases
PRIDE	P57847.
Genome annotation databases
GeneID	1243875.
GenomeReviews	Gene locus PM0528 in contig AE004439_GR.
KEGG	pmu:PM0528.
NMPDR	fig|272843.1.peg.528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57847.
OMA	RMPTIAG.
Enzyme and pathway databases
BRENDA	6.1.1.18. 258935.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_PASMU

Accession

Primary (citable) accession number: P57847

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)