Reviewed, UniProtKB/Swiss-Prot P57829 (PUR2_PASMU)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	PM0224

Organism

Pasteurella multocida [Complete proteome] [HAMAP]

Taxonomic identifier

747 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151468
Regions
	Domain	109 – 316	208	ATP-grasp
	Nucleotide binding	135 – 196	62	ATP By similarity
Sites
	Metal binding	286	1	Magnesium or manganese By similarity
	Metal binding	288	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57829-1 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 65080956ED18F865	FASTA	429	45,872
	10 20 30 40 50 60 MNILIIGNGG REHALAWKVA QSPMAEKVFV APGNAGTALE EKVENVAISA TDVEKLVAFA 70 80 90 100 110 120 QANHIGLTIV GPEAPLVIGV VDAFRAAGLK IFGPTKAAAQ LEGSKAFTKD FLARHHIPTA 130 140 150 160 170 180 EYQNFTEVEP ALAYLREKGA PIVVKADGLA AGKGVIVAMT LDEAEAAVKE MLSGNAFGEA 190 200 210 220 230 240 GSRVVIEEFL DGEEASFIVM VDGKNVEPMA TSQDHKRVGE NDTGLNTGGM GAYSPAPVVT 250 260 270 280 290 300 PEIHERIMQQ VIYPTVNGMA AEGNIYTGFL YAGLMIMPNG QPKVIEFNCR FGDPETQPIM 310 320 330 340 350 360 LRLESDLVEL CLAACDGKLD QKKSQWCEQA SLGIVLAAEG YPGDYRKGDE ITGIESAVEN 370 380 390 400 410 420 QKVFLAGVEN KDGKLVTNGG RVVCVTALGD TVYDAQQQAL ALAEQVKWTG RFYRRDIGYR AVAREKNNA

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004439 Genomic DNA. Translation: AAK02308.1.
RefSeq	NP_245161.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Proteomic databases
PRIDE	P57829.
Genome annotation databases
GeneID	1243571.
GenomeReviews	Gene locus PM0224 in contig AE004439_GR.
KEGG	pmu:PM0224.
NMPDR	fig|272843.1.peg.224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57829.
OMA	IEYNCRF.
Enzyme and pathway databases
BRENDA	6.3.4.13. 258935.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_PASMU

Accession

Primary (citable) accession number: P57829

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)