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P57829 (PUR2_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:PM0224
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151468

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
P57829 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: 65080956ED18F865

FASTA42945,872
        10         20         30         40         50         60 
MNILIIGNGG REHALAWKVA QSPMAEKVFV APGNAGTALE EKVENVAISA TDVEKLVAFA 

        70         80         90        100        110        120 
QANHIGLTIV GPEAPLVIGV VDAFRAAGLK IFGPTKAAAQ LEGSKAFTKD FLARHHIPTA 

       130        140        150        160        170        180 
EYQNFTEVEP ALAYLREKGA PIVVKADGLA AGKGVIVAMT LDEAEAAVKE MLSGNAFGEA 

       190        200        210        220        230        240 
GSRVVIEEFL DGEEASFIVM VDGKNVEPMA TSQDHKRVGE NDTGLNTGGM GAYSPAPVVT 

       250        260        270        280        290        300 
PEIHERIMQQ VIYPTVNGMA AEGNIYTGFL YAGLMIMPNG QPKVIEFNCR FGDPETQPIM 

       310        320        330        340        350        360 
LRLESDLVEL CLAACDGKLD QKKSQWCEQA SLGIVLAAEG YPGDYRKGDE ITGIESAVEN 

       370        380        390        400        410        420 
QKVFLAGVEN KDGKLVTNGG RVVCVTALGD TVYDAQQQAL ALAEQVKWTG RFYRRDIGYR 


AVAREKNNA 

« Hide

References

[1]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004439 Genomic DNA. Translation: AAK02308.1.
RefSeqNP_245161.1. NC_002663.1.

3D structure databases

ProteinModelPortalP57829.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272843.PM0224.

Proteomic databases

PRIDEP57829.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK02308; AAK02308; PM0224.
GeneID1243571.
KEGGpmu:PM0224.
PATRIC22869600. VBIPasMul88067_0232.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycPMUL272843:GC8W-228-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_PASMU
AccessionPrimary (citable) accession number: P57829
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways