P57819 (DDL_PASMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-alanine--D-alanine ligase
EC=6.3.2.4

Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase

Gene names

Name:	ddl
Synonyms:	ddlB
Ordered Locus Names:	PM0144

Organism

Pasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]

Taxonomic identifier

272843 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella › Pasteurella multocida › Pasteurella multocida subsp. multocida

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP-Rule MF_00047
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00047.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	D-alanine--D-alanine ligase HAMAP-Rule MF_00047		PRO_0000177849
Regions
	Domain	106 – 305	200	ATP-grasp
	Nucleotide binding	136 – 191	56	ATP By similarity
Sites
	Metal binding	259	1	Magnesium or manganese 1 By similarity
	Metal binding	272	1	Magnesium or manganese 1 By similarity
	Metal binding	272	1	Magnesium or manganese 2 By similarity
	Metal binding	274	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57819 [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 5207150CE602355E	FASTA	309	33,776
	10 20 30 40 50 60 MKKTLKQEKI AVLLGGTSAE REVSLDSGQA VLNALLSAGF DAHPIDPKTF PVATLKEQGF 70 80 90 100 110 120 DRVFNILHGR GGEDGTMQGL LEQIGIPYTG CGVMTSALTM DKMRTKMLWK AFGLPVAEME 130 140 150 160 170 180 IVTKANVGEL NPSAVVEKLG LPVMVKPSLE GSSVGLTKVK RVEDLKSAVD FALKYDDTVL 190 200 210 220 230 240 IEEWLSGAEF TVPVLDNEVL PSIRIVPEGE FYDYEAKYIS DNTQYFCPSG LSAEREDELK 250 260 270 280 290 300 QLVKQAYDVV GCRGWSRIDV MLDGNDAFRL VEVNTNPGMT SHSLFPKSAA TVGYSFEQLV VKILELSAE

References

[1]	"Complete genomic sequence of Pasteurella multocida Pm70." May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pm70.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004439 Genomic DNA. Translation: AAK02228.1.
RefSeq	NP_245081.1. NC_002663.1.
3D structure databases
ProteinModelPortal	P57819.
SMR	P57819. Positions 8-307.
ModBase	Search...
Protein-protein interaction databases
STRING	272843.PM0144.
Proteomic databases
PRIDE	P57819.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK02228; AAK02228; PM0144.
GeneID	1243491.
KEGG	pmu:PM0144.
PATRIC	22869432. VBIPasMul88067_0149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1181.
HOGENOM	HOG000011592.
KO	K01921.
OMA	GCRTWGR.
ProtClustDB	PRK01372.
Enzyme and pathway databases
BioCyc	PMUL272843:GC8W-147-MONOMER.
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 2 hits. 3.40.50.20. 1 hit.
HAMAP	MF_00047. Dala_Dala_lig.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom. [Graphical view]
PANTHER	PTHR23132. PTHR23132. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 2 hits. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_PASMU

Accession

Primary (citable) accession number: P57819

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	May 29, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program