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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Pasteurella multocida (strain Pm70)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259Magnesium or manganese 1UniRule annotation1
Metal bindingi272Magnesium or manganese 1UniRule annotation1
Metal bindingi272Magnesium or manganese 2UniRule annotation1
Metal bindingi274Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 191ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Synonyms:ddlB
Ordered Locus Names:PM0144
OrganismiPasteurella multocida (strain Pm70)
Taxonomic identifieri272843 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaePasteurella
Proteomesi
  • UP000000809 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001778491 – 309D-alanine--D-alanine ligaseAdd BLAST309

Proteomic databases

PRIDEiP57819.

Interactioni

Protein-protein interaction databases

STRINGi272843.PM0144.

Structurei

3D structure databases

ProteinModelPortaliP57819.
SMRiP57819.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini106 – 305ATP-graspUniRule annotationAdd BLAST200

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
HOGENOMiHOG000011592.
KOiK01921.
OMAiYETKYTE.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P57819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTLKQEKI AVLLGGTSAE REVSLDSGQA VLNALLSAGF DAHPIDPKTF
60 70 80 90 100
PVATLKEQGF DRVFNILHGR GGEDGTMQGL LEQIGIPYTG CGVMTSALTM
110 120 130 140 150
DKMRTKMLWK AFGLPVAEME IVTKANVGEL NPSAVVEKLG LPVMVKPSLE
160 170 180 190 200
GSSVGLTKVK RVEDLKSAVD FALKYDDTVL IEEWLSGAEF TVPVLDNEVL
210 220 230 240 250
PSIRIVPEGE FYDYEAKYIS DNTQYFCPSG LSAEREDELK QLVKQAYDVV
260 270 280 290 300
GCRGWSRIDV MLDGNDAFRL VEVNTNPGMT SHSLFPKSAA TVGYSFEQLV

VKILELSAE
Length:309
Mass (Da):33,776
Last modified:April 27, 2001 - v1
Checksum:i5207150CE602355E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004439 Genomic DNA. Translation: AAK02228.1.
RefSeqiWP_005723044.1. NC_002663.1.

Genome annotation databases

EnsemblBacteriaiAAK02228; AAK02228; PM0144.
GeneIDi29389553.
KEGGipmu:PM0144.

Similar proteinsi

Entry informationi

Entry nameiDDL_PASMU
AccessioniPrimary (citable) accession number: P57819
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: August 30, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families