Skip Header

Contribute Send feedback
Read comments (?) or add your own

P57808 (TYSY_PASMU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:PM0079
OrganismPasteurella multocida (strain Pm70) [Complete proteome] [HAMAP]
Taxonomic identifier272843 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Pasteurella

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008

Subunit structure

Homodimer By similarity. HAMAP MF_00008

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Thymidylate synthase HAMAP MF_00008
PRO_0000140999

Sites

Active site1601 By similarity

Experimental info

Sequence conflict211A → E in AAL54880. Ref.1
Sequence conflict2381D → E in AAL54880. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P57808 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: B89BB6F11657BD25

FASTA28332,577
        10         20         30         40         50         60 
MKHYLELCQR IVDEGVWVEN ARTGKRCLTV IDADLTYDVA NNQFPLITTR KSYWKAAIAE 

        70         80         90        100        110        120 
FLGYIRGYDN AADFRKLGTK TWDANANENS AWLNNPHRKG TDDMGRVYGV QGRAWRKPNG 

       130        140        150        160        170        180 
ETVDQLRKIV NNLRNGIDDR GEIMTFFNPG EFELGCLRPC MHTHTFSLLG DTLYLTSYQR 

       190        200        210        220        230        240 
SCDVPLGLNF NQIQVFTFLA LMAQITGKKA GKAYHKIINA HIYEDQLDLM KNVQLKRDPF 

       250        260        270        280 
PLPQLHINPE IKTLEDLETW VTMDDFKVTG YQCHDAIKYP FSV

« Hide

References

« Hide 'large scale' references
[1]Moreno J.A., Bosch M., Badiola I., Llagostera M., Barbe J.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Pm25.
[2]"Complete genomic sequence of Pasteurella multocida Pm70."
May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001) [PubMed: 11248100] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Pm70.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004439 Genomic DNA. Translation: AAK02163.1.
AF064791 Genomic DNA. Translation: AAL54880.1.
RefSeqNP_245016.1. NC_002663.1.

3D structure databases

ProteinModelPortalP57808.
ModBaseSearch...

Proteomic databases

PRIDEP57808.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1243426.
GenomeReviewsGene locus PM0079 in contig AE004439_GR.
KEGGpmu:PM0079.
NMPDRfig|272843.1.peg.79.
PATRIC22869292. VBIPasMul88067_0081.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG588098.
OMAPIKYPFS.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycPMUL272843:PM0079-MONOMER.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KOK00560.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. Thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_PASMU
AccessionPrimary (citable) accession number: P57808
Secondary accession number(s): Q8VUE1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents