Putative phospholipid-transporting ATPase 12 - Arabidopsis thaliana (Mouse-ear cress)

Names and origin

Protein names

Recommended name:
    Putative phospholipid-transporting ATPase 12
      Short name=AtALA12
    EC=3.6.3.1
Alternative name(s):
    Aminophospholipid flippase 12

Gene names

Name:	ALA12
Ordered Locus Names:	At1g26130
ORF Names:	F28B23.19, F14G11.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1184 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Involved in transport of phospholipids Potential.
Catalytic activity	ATP + H₂O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IV subfamily.
Sequence caution	The sequence AAG50529.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro phospholipid transport Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid-translocating ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1184

1184

Putative phospholipid-transporting ATPase 12

PRO_0000046396

Regions

Topological domain

1 – 75

75

Cytoplasmic Potential

Transmembrane

76 – 97

22

Potential

Topological domain

98 – 101

4

Extracellular Potential

Transmembrane

102 – 124

23

Potential

Topological domain

125 – 306

182

Cytoplasmic Potential

Transmembrane

307 – 328

22

Potential

Topological domain

329 – 364

36

Extracellular Potential

Transmembrane

365 – 382

18

Potential

Topological domain

383 – 921

539

Cytoplasmic Potential

Transmembrane

922 – 941

20

Potential

Topological domain

942 – 955

14

Extracellular Potential

Transmembrane

956 – 975

20

Potential

Topological domain

976 – 1005

30

Cytoplasmic Potential

Transmembrane

1006 – 1028

23

Potential

Topological domain

1029 – 1041

13

Extracellular Potential

Transmembrane

1042 – 1064

23

Potential

Topological domain

1065 – 1070

6

Cytoplasmic Potential

Transmembrane

1071 – 1091

21

Potential

Topological domain

1092 – 1108

17

Extracellular Potential

Transmembrane

1109 – 1133

25

Potential

Topological domain

1134 – 1184

51

Cytoplasmic Potential

Sites

Active site

430

1

4-aspartylphosphate intermediate By similarity

Metal binding

866

1

Magnesium By similarity

Metal binding

870

1

Magnesium By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P57792-1 [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: D9B6BC0C79CDAE74
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FASTA

1,184

133,793

        10         20         30         40         50         60 
MATVSGRRRK RKIQLSKLFT LTGAKACFKP DHSKIGRSGF SRVVFCNQPD SPEAESRNYC 

        70         80         90        100        110        120 
DNYVRTTKYT LATFLPKSLF EQFRRVANFY FLVVGILSFT PLAPYTAVSA IVPLTFVILA 

       130        140        150        160        170        180 
TMFKEGVEDW RRKQQDIEVN NRKVRVHRGN GNFDLREWKT LRVGDILKVE KNEFFPADLV 

       190        200        210        220        230        240 
LLSSSYEDAV CYVETMNLDG ETNLKLKQGL EVTLSLREEL NFRDFEAFIK CEDPNANLYS 

       250        260        270        280        290        300 
FVGTMDLKGE KYPLSPQQLL LRGSKLRNTD YIYGVVIFTG PDTKVVQNST DPPSKRSMIE 

       310        320        330        340        350        360 
RKMDKIIYLM FLMVFSLAFF GSVLFGIWTR DDFQNGVMER WYLKPDDSSI FFDPKRAPMA 

       370        380        390        400        410        420 
AIYHFLTALM LNSYFIPISL YVSIEIVKVL QSIFINQDIH MYYEEADKPA HARTSNLNEE 

       430        440        450        460        470        480 
LGQVGTILSD KTGTLTCNSM EFIKCSIAGT AYGRGVTEVE MAMDKRKGSA LVNQSNGNST 

       490        500        510        520        530        540 
EDAVAAEPAV KGFNFRDERI MDGNWVTETH ADVIQKFFQL LAVCHTVIPE VDEDTGKISY 

       550        560        570        580        590        600 
EAESPDEAAF VIAARELGFE FFTRTQTTIS VRELDLVTGE RVERLYSVLN VLEFSSSKKR 

       610        620        630        640        650        660 
MSVIVQDQDG KLLLLCKGAD SVMFERLSES GRKYEKETRD HVNEYADAGL RTLILAYREL 

       670        680        690        700        710        720 
DENEYEVFTE RISEAKNSVS ADREALIDEV TEKIEKNLVL LGATAVEDKL QNGVPDCINK 

       730        740        750        760        770        780 
LAQAGIKIWV LTGDKMETAI NIGFACSLLR RDMKQIIINL ETPEIQQLEK SGEKDAIAAL 

       790        800        810        820        830        840 
KENVLHQITS GKAQLKASGG NAKAFALIID GKSLAYALEE DMKGIFLELA IGCASVICCR 

       850        860        870        880        890        900 
SSPKQKALVT RLVKTGSGQT TLAIGDGAND VGMLQEADIG VGISGVEGMQ AVMSSDIAIA 

       910        920        930        940        950        960 
QFRYLERLLL VHGHWCYRRI SKMICYFFYK NITFGFTLFL YEAYTSFSAT PAYNDWYLSL 

       970        980        990       1000       1010       1020 
YSVFFTSLPV ICLGIFDQDV SAPFCLKFPV LYQEGVQNLL FSWRRILSWM FHGFCSAIII 

      1030       1040       1050       1060       1070       1080 
FFLCKTSLES QAFNHEGKTA GRDILGGTMY TCVVWVVSLQ MVLTISYFTL IQHVVVWGSV 

      1090       1100       1110       1120       1130       1140 
VIWYLFLMVY GSLPIRMSTD AYMVFLEALA PAPSYWITTL FVVLSTMMPY FIFSAIQMRF 

      1150       1160       1170       1180 
FPMSHGTVQL LRYEDQCSNS GNFEMGRQGS VRPTLVMRSH QPES

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References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.

Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

+

Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AC079829 Genomic DNA. Translation: AAG50692.1. AC084221 Genomic DNA. Translation: AAG50529.1. Sequence problems.
IPI	IPI00519052.
PIR	D86387.
RefSeq	NP_173938.1.
UniGene	At.49917
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P57792.
Proteomic databases
PRIDE	P57792.
Genome annotation databases
GeneID	839154.
GenomeReviews	Gene locus AT1G26130 in contig CT485782_GR.
KEGG	ath:AT1G26130.
NMPDR	fig\|3702.1.peg.2929.
Organism-specific databases
TAIR	At1g26130.
Phylogenomic databases
eggNOG	KOG0206.
HOGENOM	HBG745019.
InParanoid	P57792.
OMA	FTERISE.
PhylomeDB	P57792.
Enzyme and pathway databases
BRENDA	3.6.3.1. 302.
Gene expression databases
ArrayExpress	P57792.
Genevestigator	P57792.
GermOnline	AT1G26130. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ALA12_ARATH

Accession

Primary (citable) accession number: P57792

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents