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P57790

- KEAP1_RAT

UniProt

P57790 - KEAP1_RAT

Protein

Kelch-like ECH-associated protein 1

Gene

Keap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome By similarity.By similarity

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: RGD

    GO - Biological processi

    1. alkanesulfonate metabolic process Source: RGD
    2. cellular response to organic cyclic compound Source: RGD
    3. cytoplasmic sequestering of transcription factor Source: RGD
    4. flavonoid metabolic process Source: RGD
    5. malate metabolic process Source: RGD
    6. negative regulation of gene expression Source: RGD
    7. proteasomal ubiquitin-independent protein catabolic process Source: UniProtKB
    8. protein oligomerization Source: RGD
    9. protein ubiquitination Source: UniProtKB
    10. regulation of transcription, DNA-templated Source: UniProtKB-KW
    11. response to immobilization stress Source: RGD
    12. response to metal ion Source: RGD
    13. response to thyroid hormone Source: RGD
    14. selenium compound metabolic process Source: RGD
    15. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kelch-like ECH-associated protein 1
    Alternative name(s):
    Cytosolic inhibitor of Nrf2
    Short name:
    INrf2
    Gene namesi
    Name:Keap1
    Synonyms:Inrf2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621619. Keap1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Shuttles between cytoplasm and nucleus.By similarity

    GO - Cellular componenti

    1. cell-cell adherens junction Source: RGD
    2. Cul3-RING ubiquitin ligase complex Source: UniProtKB
    3. cytoplasm Source: RGD
    4. focal adhesion Source: RGD
    5. nucleus Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 624624Kelch-like ECH-associated protein 1PRO_0000119095Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and is subject to proteasomal-independent degradation. Quinone-induced oxidative stress, but not sulforaphane, increases its ubiquitination. Ubiquitination and subsequent degradation is most pronounced following prolonged exposure of cells to oxidative stress, particularly in glutathione-deficient cells that are highly susceptible to oxidative stress By similarity.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiP57790.
    PRIDEiP57790.

    PTM databases

    PhosphoSiteiP57790.

    Expressioni

    Gene expression databases

    GenevestigatoriP57790.

    Interactioni

    Subunit structurei

    Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1 By similarity. Interacts indirectly with ENC1 By similarity. Interacts with MAP1LC3B By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250741. 1 interaction.
    STRINGi10116.ENSRNOP00000028360.

    Structurei

    3D structure databases

    ProteinModelPortaliP57790.
    SMRiP57790. Positions 324-619.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 14973BTBPROSITE-ProRule annotationAdd
    BLAST
    Domaini184 – 286103BACKAdd
    BLAST
    Repeati327 – 37246Kelch 1Add
    BLAST
    Repeati373 – 42351Kelch 2Add
    BLAST
    Repeati424 – 47047Kelch 3Add
    BLAST
    Repeati471 – 51747Kelch 4Add
    BLAST
    Repeati519 – 56446Kelch 5Add
    BLAST
    Repeati565 – 61147Kelch 6Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi301 – 31010Nuclear export signalBy similarity

    Domaini

    The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.By similarity

    Sequence similaritiesi

    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 6 Kelch repeats.Curated

    Keywords - Domaini

    Kelch repeat, Repeat

    Phylogenomic databases

    eggNOGiNOG255039.
    HOGENOMiHOG000230814.
    HOVERGENiHBG014286.
    InParanoidiP57790.
    PhylomeDBiP57790.

    Family and domain databases

    Gene3Di2.130.10.80. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR011705. BACK.
    IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR015916. Gal_Oxidase_b-propeller.
    IPR017096. Kelch-like_gigaxonin-typ.
    IPR006652. Kelch_1.
    [Graphical view]
    PfamiPF07707. BACK. 1 hit.
    PF00651. BTB. 1 hit.
    PF01344. Kelch_1. 6 hits.
    [Graphical view]
    PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
    SMARTiSM00875. BACK. 1 hit.
    SM00225. BTB. 1 hit.
    SM00612. Kelch. 6 hits.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57790-1 [UniParc]FASTAAdd to Basket

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    MQPEPKPSGA PRSSQFLPLW SKCPEGAGDA VMYASTECKA EVTPSQDGNR    50
    TFSYTLEDHT KQAFGIMNEL RLSQQLCDVT LQVKYEDIPA AQFMAHKVVL 100
    ASSSPVFKAM FTNGLREQGM EVVSIEGIHP KVMERLIEFA YTASISVGEK 150
    CVLHVMNGAV MYQIDSVVRA CSDFLVQQLD PSNAIGIANF AEQIGCTELH 200
    QRAREYIYMH FGEVAKQEEF FNLSHCQLAT LISRDDLNVR CESEVFHACI 250
    DWVKYDCPQR RFYVQALLRA VRCHALTPRF LQTQLQKCEI LQADARCKDY 300
    LVQIFQELTL HKPTQAVPCR APKVGRLIYT AGGYFRQSLS YLEAYNPSNG 350
    SWLRLADLQV PRSGLAGCVV GGLLYAVGGR NNSPDGNTDS SALDCYNPMT 400
    NQWSPCASLS VPRNRSGGGV IDGHIYAVGG SHGCIHHSSV ERYEPDRDEW 450
    HLVAPMLTRR IGVGVAVLNR LLYAVGGFDG TNRLNSAECY YPERNEWRMI 500
    TPMNTIRSGA GVCVLHSCIY AAGGYDGQDQ LNSVERYDVE TETWTFVASM 550
    KHRRSALGIA VHQGRIYVLG GYDGHTFLDS VECYDPDTDT WSEVTRLTSG 600
    RSGVGVAVTM EPCRKQIDQQ NCTC 624
    Length:624
    Mass (Da):69,399
    Last modified:April 27, 2001 - v1
    Checksum:iB5E77B5A72546A79
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF304364 mRNA. Translation: AAG16275.1.
    UniGeneiRn.23467.

    Genome annotation databases

    UCSCiRGD:621619. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF304364 mRNA. Translation: AAG16275.1 .
    UniGenei Rn.23467.

    3D structure databases

    ProteinModelPortali P57790.
    SMRi P57790. Positions 324-619.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250741. 1 interaction.
    STRINGi 10116.ENSRNOP00000028360.

    PTM databases

    PhosphoSitei P57790.

    Proteomic databases

    PaxDbi P57790.
    PRIDEi P57790.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:621619. rat.

    Organism-specific databases

    RGDi 621619. Keap1.

    Phylogenomic databases

    eggNOGi NOG255039.
    HOGENOMi HOG000230814.
    HOVERGENi HBG014286.
    InParanoidi P57790.
    PhylomeDBi P57790.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 620287.
    PROi P57790.

    Gene expression databases

    Genevestigatori P57790.

    Family and domain databases

    Gene3Di 2.130.10.80. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR011705. BACK.
    IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR015916. Gal_Oxidase_b-propeller.
    IPR017096. Kelch-like_gigaxonin-typ.
    IPR006652. Kelch_1.
    [Graphical view ]
    Pfami PF07707. BACK. 1 hit.
    PF00651. BTB. 1 hit.
    PF01344. Kelch_1. 6 hits.
    [Graphical view ]
    PIRSFi PIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
    SMARTi SM00875. BACK. 1 hit.
    SM00225. BTB. 1 hit.
    SM00612. Kelch. 6 hits.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional characterization and role of INrf2 in antioxidant response element-mediated expression and antioxidant induction of NAD(P)H:quinone oxidoreductase1 gene."
      Dhakshinamoorthy S., Jaiswal A.K.
      Oncogene 20:3906-3917(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiKEAP1_RAT
    AccessioniPrimary (citable) accession number: P57790
    Secondary accession number(s): Q9ERI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3