P57789 (KCNKA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 10

Alternative name(s):
Outward rectifying potassium channel protein TREK-2
TREK-2 K(+) channel subunit

Gene names

Name:	KCNK10
Synonyms:	TREK2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K+ currents. Activated by arachidonic acid and other naturally occurring unsaturated free fatty acids.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Abundantly expressed in pancreas and kidney and to a lower level in brain, testis, colon, and small intestine. Isoform b is strongly expressed in kidney (primarily in the proximal tubule) and pancreas, whereas isoform c is abundantly expressed in brain.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Non-traceable author statement Ref.1. Source: ProtInc synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	potassium channel activity Traceable author statement Ref.1. Source: ProtInc voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P57789-1)

Also known as: TREK-2a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P57789-4)

Also known as: TREK-2b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MFFLYTDFFLSL → MEDGFKGDRTEGCRSDS

Isoform C (identifier: P57789-3)

Also known as: TREK-2c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MFFLYTDFFLSL → MKFPIETPRKQVNWDPK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Potassium channel subfamily K member 10		PRO_0000101758
Regions
	Topological domain	1 – 71	71	Cytoplasmic Potential
	Transmembrane	72 – 92	21	Helical; Potential
	Intramembrane	154 – 180	27	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	182 – 202	21	Helical; Potential
	Topological domain	203 – 233	31	Cytoplasmic Potential
	Transmembrane	234 – 254	21	Helical; Potential
	Intramembrane	263 – 294	32	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	299 – 319	21	Helical; Potential
	Topological domain	320 – 538	219	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 12	12	MFFLY…FFLSL → MEDGFKGDRTEGCRSDS in isoform B.		VSP_006697
	Alternative sequence	1 – 12	12	MFFLY…FFLSL → MKFPIETPRKQVNWDPK in isoform C.		VSP_006698
	Natural variant	70	1	K → Q. Corresponds to variant rs398263 [ dbSNP | Ensembl ].		VAR_060216
	Natural variant	512	1	A → T. Ref.4 Corresponds to variant rs17762463 [ dbSNP | Ensembl ].		VAR_052428
Experimental info
	Sequence conflict	529	1	E → G in AAL95705. Ref.2
	Sequence conflict	529	1	E → G in AAL95706. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (TREK-2a) [UniParc]. Last modified April 27, 2001. Version 1. Checksum: 8EA615B08D147FBC	FASTA	538	59,765
	10 20 30 40 50 60 MFFLYTDFFL SLVAVPAAAP VCQPKSATNG QPPAPAPTPT PRLSISSRAT VVARMEGTSQ 70 80 90 100 110 120 GGLQTVMKWK TVVAIFVVVV VYLVTGGLVF RALEQPFESS QKNTIALEKA EFLRDHVCVS 130 140 150 160 170 180 PQELETLIQH ALDADNAGVS PIGNSSNNSS HWDLGSAFFF AGTVITTIGY GNIAPSTEGG 190 200 210 220 230 240 KIFCILYAIF GIPLFGFLLA GIGDQLGTIF GKSIARVEKV FRKKQVSQTK IRVISTILFI 250 260 270 280 290 300 LAGCIVFVTI PAVIFKYIEG WTALESIYFV VVTLTTVGFG DFVAGGNAGI NYREWYKPLV 310 320 330 340 350 360 WFWILVGLAY FAAVLSMIGD WLRVLSKKTK EEVGEIKAHA AEWKANVTAE FRETRRRLSV 370 380 390 400 410 420 EIHDKLQRAA TIRSMERRRL GLDQRAHSLD MLSPEKRSVF AALDTGRFKA SSQESINNRP 430 440 450 460 470 480 NNLRLKGPEQ LNKHGQGASE DNIINKFGST SRLTKRKNKD LKKTLPEDVQ KIYKTFRNYS 490 500 510 520 530 LDEEKKEEET EKMCNSDNSS TAMLTDCIQQ HAELENGMIP TDTKDREPEN NSLLEDRN
	Isoform B (TREK-2b) [UniParc]. Checksum: 17DB1AFAFAB07C46	FASTA	543	60,111
	10 20 30 40 50 60 MEDGFKGDRT EGCRSDSVAV PAAAPVCQPK SATNGQPPAP APTPTPRLSI SSRATVVARM 70 80 90 100 110 120 EGTSQGGLQT VMKWKTVVAI FVVVVVYLVT GGLVFRALEQ PFESSQKNTI ALEKAEFLRD 130 140 150 160 170 180 HVCVSPQELE TLIQHALDAD NAGVSPIGNS SNNSSHWDLG SAFFFAGTVI TTIGYGNIAP 190 200 210 220 230 240 STEGGKIFCI LYAIFGIPLF GFLLAGIGDQ LGTIFGKSIA RVEKVFRKKQ VSQTKIRVIS 250 260 270 280 290 300 TILFILAGCI VFVTIPAVIF KYIEGWTALE SIYFVVVTLT TVGFGDFVAG GNAGINYREW 310 320 330 340 350 360 YKPLVWFWIL VGLAYFAAVL SMIGDWLRVL SKKTKEEVGE IKAHAAEWKA NVTAEFRETR 370 380 390 400 410 420 RRLSVEIHDK LQRAATIRSM ERRRLGLDQR AHSLDMLSPE KRSVFAALDT GRFKASSQES 430 440 450 460 470 480 INNRPNNLRL KGPEQLNKHG QGASEDNIIN KFGSTSRLTK RKNKDLKKTL PEDVQKIYKT 490 500 510 520 530 540 FRNYSLDEEK KEEETEKMCN SDNSSTAMLT DCIQQHAELE NGMIPTDTKD REPENNSLLE DRN
	Isoform C (TREK-2c) [UniParc]. Checksum: B158DF9AF076444D	FASTA	543	60,335
	10 20 30 40 50 60 MKFPIETPRK QVNWDPKVAV PAAAPVCQPK SATNGQPPAP APTPTPRLSI SSRATVVARM 70 80 90 100 110 120 EGTSQGGLQT VMKWKTVVAI FVVVVVYLVT GGLVFRALEQ PFESSQKNTI ALEKAEFLRD 130 140 150 160 170 180 HVCVSPQELE TLIQHALDAD NAGVSPIGNS SNNSSHWDLG SAFFFAGTVI TTIGYGNIAP 190 200 210 220 230 240 STEGGKIFCI LYAIFGIPLF GFLLAGIGDQ LGTIFGKSIA RVEKVFRKKQ VSQTKIRVIS 250 260 270 280 290 300 TILFILAGCI VFVTIPAVIF KYIEGWTALE SIYFVVVTLT TVGFGDFVAG GNAGINYREW 310 320 330 340 350 360 YKPLVWFWIL VGLAYFAAVL SMIGDWLRVL SKKTKEEVGE IKAHAAEWKA NVTAEFRETR 370 380 390 400 410 420 RRLSVEIHDK LQRAATIRSM ERRRLGLDQR AHSLDMLSPE KRSVFAALDT GRFKASSQES 430 440 450 460 470 480 INNRPNNLRL KGPEQLNKHG QGASEDNIIN KFGSTSRLTK RKNKDLKKTL PEDVQKIYKT 490 500 510 520 530 540 FRNYSLDEEK KEEETEKMCN SDNSSTAMLT DCIQQHAELE NGMIPTDTKD REPENNSLLE DRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human TREK2, a 2P domain mechano-sensitive K+ channel with multiple regulations by polyunsaturated fatty acids, lysophospholipids and Gs, Gi, and Gq protein-coupled receptors." Lesage F., Terrenoire C., Romey G., Lazdunski M. J. Biol. Chem. 275:28398-28405(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"Expression pattern and functional characteristics of two novel splice variants of the two-pore-domain potassium channel TREK-2." Gu W., Schlichthorl G., Hirsch J.R., Engels H., Karschin C., Karschin A., Derst C., Steinlein O.K., Daut J. J. Physiol. (Lond.) 539:657-668(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C).
[3]	"Regulation of two-pore-domain (K2P) potassium leak channels by the tyrosine kinase inhibitor genistein." Gierten J., Ficker E., Bloehs R., Schlomer K., Kathofer S., Scholz E., Zitron E., Kiesecker C., Bauer A., Becker R., Katus H.A., Karle C.A., Thomas D. Br. J. Pharmacol. 154:1680-1690(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C). Tissue: Brain, Kidney and Pancreas.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C), VARIANT THR-512. Tissue: Brain and Cerebellum.
[5]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF279890 mRNA. Translation: AAG15191.1. AF385399 mRNA. Translation: AAL95705.1. AF385400 mRNA. Translation: AAL95706.1. EU978938 mRNA. Translation: ACH86097.1. EU978939 mRNA. Translation: ACH86098.1. EU978940 mRNA. Translation: ACH86099.1. EU978941 mRNA. Translation: ACH86100.1. AK313499 mRNA. Translation: BAG36281.1. AK315263 mRNA. Translation: BAG37680.1. AL049834 Genomic DNA. No translation available. AL133279 Genomic DNA. No translation available. CH471061 Genomic DNA. Translation: EAW81373.1. CH471061 Genomic DNA. Translation: EAW81374.1. CH471061 Genomic DNA. Translation: EAW81375.1. BC075021 mRNA. Translation: AAH75021.1. BC075022 mRNA. Translation: AAH75022.1.
IPI	IPI00028078. IPI00217521. IPI00465069.
RefSeq	NP_066984.1. NM_021161.4. NP_612190.1. NM_138317.2. NP_612191.1. NM_138318.2.
UniGene	Hs.560255.
3D structure databases
ProteinModelPortal	P57789.
ModBase	Search...
Protein-protein interaction databases
IntAct	P57789. 1 interaction.
MINT	MINT-4050532.
STRING	9606.ENSP00000310568.
PTM databases
PhosphoSite	P57789.
Polymorphism databases
DMDM	13431412.
Proteomic databases
PaxDb	P57789.
PRIDE	P57789.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312350; ENSP00000310568; ENSG00000100433. ENST00000319231; ENSP00000312811; ENSG00000100433. ENST00000340700; ENSP00000343104; ENSG00000100433.
GeneID	54207.
KEGG	hsa:54207.
UCSC	uc001xwo.3. human.
Organism-specific databases
CTD	54207.
GeneCards	GC14M088927.
HGNC	HGNC:6273. KCNK10.
HPA	HPA030462.
MIM	605873. gene.
neXtProt	NX_P57789.
PharmGKB	PA30053.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG308296.
HOGENOM	HOG000013106.
HOVERGEN	HBG052234.
KO	K04920.
OMA	EQLNKHG.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	P57789.
Bgee	P57789.
CleanEx	HS_KCNK10.
Genevestigator	P57789.
GermOnline	ENSG00000100433. Homo sapiens.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR003976. 2pore_dom_K_chnl_TREK. IPR013099. Ion_trans_2. [Graphical view]
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PRINTS	PR01333. 2POREKCHANEL. PR01499. TREKCHANNEL.
ProtoNet	Search...
Other
GenomeRNAi	54207.
NextBio	56534.
SOURCE	Search...

Entry information

Entry name

KCNKA_HUMAN

Accession

Primary (citable) accession number: P57789
Secondary accession number(s): B2R8T4 , B2RCT3, B5TJL4, Q6B014, Q8TDK7, Q8TDK8, Q9HB59

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.