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P57789 (KCNKA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel subfamily K member 10
Alternative name(s):
Outward rectifying potassium channel protein TREK-2
TREK-2 K(+) channel subunit
Gene names
Name:KCNK10
Synonyms:TREK2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length538 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K+ currents. Activated by arachidonic acid and other naturally occurring unsaturated free fatty acids.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Abundantly expressed in pancreas and kidney and to a lower level in brain, testis, colon, and small intestine. Isoform bis strongly expressed in kidney (primarily in the proximal tubule) and pancreas, whereas isoform cis abundantly expressed in brain.

Sequence similarities

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P57789-1)

Also known as: TREK-2a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: P57789-4)

Also known as: TREK-2b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MFFLYTDFFLSL → MEDGFKGDRTEGCRSDS
Isoform C (identifier: P57789-3)

Also known as: TREK-2c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MFFLYTDFFLSL → MKFPIETPRKQVNWDPK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 538538Potassium channel subfamily K member 10
PRO_0000101758

Regions

Topological domain1 – 7171Cytoplasmic Potential
Transmembrane72 – 9221Helical; Potential
Intramembrane154 – 18027Pore-forming; Name=Pore-forming 1; Potential
Transmembrane182 – 20221Helical; Potential
Topological domain203 – 23331Cytoplasmic Potential
Transmembrane234 – 25421Helical; Potential
Intramembrane263 – 29432Pore-forming; Name=Pore-forming 2; Potential
Transmembrane299 – 31921Helical; Potential
Topological domain320 – 538219Cytoplasmic Potential

Amino acid modifications

Glycosylation1441N-linked (GlcNAc...) Potential
Glycosylation1471N-linked (GlcNAc...) Potential
Glycosylation1481N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 1212MFFLY…FFLSL → MEDGFKGDRTEGCRSDS in isoform B.
VSP_006697
Alternative sequence1 – 1212MFFLY…FFLSL → MKFPIETPRKQVNWDPK in isoform C.
VSP_006698
Natural variant701K → Q.
Corresponds to variant rs398263 [ dbSNP | Ensembl ].
VAR_060216
Natural variant5121A → T. Ref.4
Corresponds to variant rs17762463 [ dbSNP | Ensembl ].
VAR_052428

Experimental info

Sequence conflict5291E → G in AAL95705. Ref.2
Sequence conflict5291E → G in AAL95706. Ref.2

Secondary structure

.................... 538
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform A (TREK-2a) [UniParc].

Last modified April 27, 2001. Version 1.
Checksum: 8EA615B08D147FBC

FASTA53859,765
        10         20         30         40         50         60 
MFFLYTDFFL SLVAVPAAAP VCQPKSATNG QPPAPAPTPT PRLSISSRAT VVARMEGTSQ 

        70         80         90        100        110        120 
GGLQTVMKWK TVVAIFVVVV VYLVTGGLVF RALEQPFESS QKNTIALEKA EFLRDHVCVS 

       130        140        150        160        170        180 
PQELETLIQH ALDADNAGVS PIGNSSNNSS HWDLGSAFFF AGTVITTIGY GNIAPSTEGG 

       190        200        210        220        230        240 
KIFCILYAIF GIPLFGFLLA GIGDQLGTIF GKSIARVEKV FRKKQVSQTK IRVISTILFI 

       250        260        270        280        290        300 
LAGCIVFVTI PAVIFKYIEG WTALESIYFV VVTLTTVGFG DFVAGGNAGI NYREWYKPLV 

       310        320        330        340        350        360 
WFWILVGLAY FAAVLSMIGD WLRVLSKKTK EEVGEIKAHA AEWKANVTAE FRETRRRLSV 

       370        380        390        400        410        420 
EIHDKLQRAA TIRSMERRRL GLDQRAHSLD MLSPEKRSVF AALDTGRFKA SSQESINNRP 

       430        440        450        460        470        480 
NNLRLKGPEQ LNKHGQGASE DNIINKFGST SRLTKRKNKD LKKTLPEDVQ KIYKTFRNYS 

       490        500        510        520        530 
LDEEKKEEET EKMCNSDNSS TAMLTDCIQQ HAELENGMIP TDTKDREPEN NSLLEDRN 

« Hide

Isoform B (TREK-2b) [UniParc].

Checksum: 17DB1AFAFAB07C46
Show »

FASTA54360,111
Isoform C (TREK-2c) [UniParc].

Checksum: B158DF9AF076444D
Show »

FASTA54360,335

References

« Hide 'large scale' references
[1]"Human TREK2, a 2P domain mechano-sensitive K+ channel with multiple regulations by polyunsaturated fatty acids, lysophospholipids and Gs, Gi, and Gq protein-coupled receptors."
Lesage F., Terrenoire C., Romey G., Lazdunski M.
J. Biol. Chem. 275:28398-28405(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]"Expression pattern and functional characteristics of two novel splice variants of the two-pore-domain potassium channel TREK-2."
Gu W., Schlichthorl G., Hirsch J.R., Engels H., Karschin C., Karschin A., Derst C., Steinlein O.K., Daut J.
J. Physiol. (Lond.) 539:657-668(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C).
[3]"Regulation of two-pore-domain (K2P) potassium leak channels by the tyrosine kinase inhibitor genistein."
Gierten J., Ficker E., Bloehs R., Schlomer K., Kathofer S., Scholz E., Zitron E., Kiesecker C., Bauer A., Becker R., Katus H.A., Karle C.A., Thomas D.
Br. J. Pharmacol. 154:1680-1690(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
Tissue: Brain, Kidney and Pancreas.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C), VARIANT THR-512.
Tissue: Brain and Cerebellum.
[5]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF279890 mRNA. Translation: AAG15191.1.
AF385399 mRNA. Translation: AAL95705.1.
AF385400 mRNA. Translation: AAL95706.1.
EU978938 mRNA. Translation: ACH86097.1.
EU978939 mRNA. Translation: ACH86098.1.
EU978940 mRNA. Translation: ACH86099.1.
EU978941 mRNA. Translation: ACH86100.1.
AK313499 mRNA. Translation: BAG36281.1.
AK315263 mRNA. Translation: BAG37680.1.
AL049834 Genomic DNA. No translation available.
AL133279 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81373.1.
CH471061 Genomic DNA. Translation: EAW81374.1.
CH471061 Genomic DNA. Translation: EAW81375.1.
BC075021 mRNA. Translation: AAH75021.1.
BC075022 mRNA. Translation: AAH75022.1.
CCDSCCDS9880.1. [P57789-1]
CCDS9881.1. [P57789-3]
CCDS9882.1. [P57789-4]
RefSeqNP_066984.1. NM_021161.4. [P57789-1]
NP_612190.1. NM_138317.2. [P57789-3]
NP_612191.1. NM_138318.2. [P57789-4]
UniGeneHs.560255.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BW5X-ray3.20A/B/C/D62-335[»]
ProteinModelPortalP57789.
SMRP57789. Positions 68-327.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119924. 2 interactions.
IntActP57789. 2 interactions.
MINTMINT-4050532.
STRING9606.ENSP00000310568.

Chemistry

ChEMBLCHEMBL2331041.
GuidetoPHARMACOLOGY521.

PTM databases

PhosphoSiteP57789.

Polymorphism databases

DMDM13431412.

Proteomic databases

PaxDbP57789.
PRIDEP57789.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000312350; ENSP00000310568; ENSG00000100433. [P57789-4]
ENST00000319231; ENSP00000312811; ENSG00000100433. [P57789-3]
ENST00000340700; ENSP00000343104; ENSG00000100433. [P57789-1]
GeneID54207.
KEGGhsa:54207.
UCSCuc001xwm.3. human. [P57789-4]
uc001xwn.3. human. [P57789-3]
uc001xwo.3. human. [P57789-1]

Organism-specific databases

CTD54207.
GeneCardsGC14M089829.
HGNCHGNC:6273. KCNK10.
HPAHPA030462.
MIM605873. gene.
neXtProtNX_P57789.
PharmGKBPA30053.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG308296.
HOGENOMHOG000013106.
HOVERGENHBG052234.
KOK04920.
OMAQLNKHGQ.
OrthoDBEOG77M8NK.
PhylomeDBP57789.
TreeFamTF313947.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

ArrayExpressP57789.
BgeeP57789.
CleanExHS_KCNK10.
GenevestigatorP57789.

Family and domain databases

InterProIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR003976. 2pore_dom_K_chnl_TREK.
[Graphical view]
PfamPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSPR01333. 2POREKCHANEL.
PR01499. TREKCHANNEL.
ProtoNetSearch...

Other

GeneWikiKCNK10.
GenomeRNAi54207.
NextBio56534.
PROP57789.
SOURCESearch...

Entry information

Entry nameKCNKA_HUMAN
AccessionPrimary (citable) accession number: P57789
Secondary accession number(s): B2R8T4 expand/collapse secondary AC list , B2RCT3, B5TJL4, Q6B014, Q8TDK7, Q8TDK8, Q9HB59
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM