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Protein

Alpha-actinin-4

Gene

Actn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (PubMed:18332111). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi779 – 790121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi820 – 831122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-4Curated
Alternative name(s):
Non-muscle alpha-actinin 4Curated
Gene namesi
Name:Actn4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1890773. Actn4.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cell junction 1 Publication

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers.By similarity

GO - Cellular componenti

  • cell-cell junction Source: UniProtKB
  • cortical cytoskeleton Source: MGI
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • focal adhesion Source: MGI
  • intracellular Source: UniProtKB
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • neuron projection Source: Ensembl
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: MGI
  • protein complex Source: MGI
  • pseudopodium Source: UniProtKB
  • stress fiber Source: UniProtKB
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Alpha-actinin-4PRO_0000073441Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei115 – 1151N6-acetyllysineBy similarity
Modified residuei250 – 2501PhosphothreonineBy similarity
Modified residuei593 – 5931N6-acetyllysineBy similarity
Modified residuei626 – 6261N6-acetyllysineBy similarity
Modified residuei780 – 7801N6-acetyllysineCombined sources
Modified residuei860 – 8601N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP57780.
PaxDbiP57780.
PeptideAtlasiP57780.
PRIDEiP57780.

PTM databases

iPTMnetiP57780.
PhosphoSiteiP57780.
SwissPalmiP57780.

Expressioni

Gene expression databases

BgeeiP57780.
CleanExiMM_ACTN4.
ExpressionAtlasiP57780. baseline and differential.
GenevisibleiP57780. MM.

Interactioni

Subunit structurei

Homodimer; antiparallel (By similarity). Interacts with BAIAP1 and PDLIM2 (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Binds TRIM3 at the N-terminus. Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation. Interacts with PPARG and RARA (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Micall2Q3TN349EBI-445071,EBI-1779852
Tjp1P394476EBI-445071,EBI-79508

GO - Molecular functioni

  • actin filament binding Source: UniProtKB
  • ion channel binding Source: MGI
  • nuclear hormone receptor binding Source: MGI
  • protein homodimerization activity Source: UniProtKB
  • retinoic acid receptor binding Source: MGI

Protein-protein interaction databases

BioGridi208624. 10 interactions.
IntActiP57780. 16 interactions.
MINTiMINT-136628.
STRINGi10090.ENSMUSP00000066068.

Structurei

3D structure databases

ProteinModelPortaliP57780.
SMRiP57780. Positions 47-910.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 267267Actin-bindingAdd
BLAST
Domaini51 – 155105CH 1PROSITE-ProRule annotationAdd
BLAST
Domaini164 – 267104CH 2PROSITE-ProRule annotationAdd
BLAST
Repeati294 – 404111Spectrin 1Add
BLAST
Repeati414 – 519106Spectrin 2Add
BLAST
Repeati529 – 640112Spectrin 3Add
BLAST
Repeati650 – 753104Spectrin 4Add
BLAST
Domaini766 – 80136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini807 – 84236EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni178 – 19316Polyphosphoinositide (PIP2)-bindingSequence analysisAdd
BLAST
Regioni737 – 912176Mediates interaction with MICALL21 PublicationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi85 – 895LXXLL motifBy similarity

Domaini

Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.By similarity

Sequence similaritiesi

Belongs to the alpha-actinin family.Curated
Contains 1 actin-binding domain.Curated
Contains 2 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 4 spectrin repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiP57780.
KOiK05699.
OMAiIVGPWIQ.
OrthoDBiEOG72C4ZJ.
PhylomeDBiP57780.
TreeFamiTF352676.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR029637. ACTN4.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PANTHERiPTHR11915:SF271. PTHR11915:SF271. 1 hit.
PfamiPF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDYHAANQA YQYGPNSGGG NGAGGGGSMG DYMAQEDDWD RDLLLDPAWE
60 70 80 90 100
KQQRKTFTAW CNSHLRKAGT QIENIDEDFR DGLKLMLLLE VISGERLPKP
110 120 130 140 150
ERGKMRVHKI NNVNKALDFI ASKGVKLVSI GAEEIVDGNA KMTLGMIWTI
160 170 180 190 200
ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNVQNFH ISWKDGLAFN
210 220 230 240 250
ALIHRHRPEL IEYDKLRKDD PVTNLNNAFE VAEKYLDIPK MLDAEDIVNT
260 270 280 290 300
ARPDEKAIMT YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEHLMEDYE
310 320 330 340 350
RLASDLLEWI RRTIPWLEDR VPQKTIQEMQ QKLEDFRDYR RVHKPPKVQE
360 370 380 390 400
KCQLEINFNT LQTKLRLSNR PAFMPSEGRM VSDINNGWQH LEQAEKGYEE
410 420 430 440 450
WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLKQR DYETATLSDI
460 470 480 490 500
KALIRKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSH NVNTRCQKIC
510 520 530 540 550
DQWDNLGSLT HSRREALEKT EKQLETIDQL HLEYAKRAAP FNNWMESAME
560 570 580 590 600
DLQDMFIVHT IEEIEGLISA HDQFKSTLPD ADREREAILA IHKEAQRIAE
610 620 630 640 650
SNHIKLSGSN PYTTVTPQII NSKWEKVQQL VPKRDHALLE EQSKQQSNEH
660 670 680 690 700
LRRQFASQAN MVGPWIQTKM EEIGRISIEM NGTLEDQLSH LKQYERSIVD
710 720 730 740 750
YKPSLDLLEQ QHQLIQEALI FDNKHTNYTM EHIRVGWEQL LTTIARTINE
760 770 780 790 800
VENQILTRDA KGISQEQMQE FRASFNHFDK DHGGALGPEE FKACLISLGY
810 820 830 840 850
DVENDRQGDA EFNRIMSVVD PNHSGLVTFQ AFIDFMSRET TDTDTADQVI
860 870 880 890 900
ASFKVLAGDK NFITAEELRR ELPPDQAEYC IARMAPYQGP DAAPGALDYK
910
SFSTALYGES DL
Length:912
Mass (Da):104,977
Last modified:February 21, 2001 - v1
Checksum:i07AA9C92AC228B5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ289242 mRNA. Translation: CAC10069.1.
BC013616 mRNA. Translation: AAH13616.1.
BC087554 mRNA. Translation: AAH87554.1.
CCDSiCCDS21061.1.
RefSeqiNP_068695.1. NM_021895.2.
UniGeneiMm.81144.

Genome annotation databases

EnsembliENSMUST00000068045; ENSMUSP00000066068; ENSMUSG00000054808.
GeneIDi60595.
KEGGimmu:60595.
UCSCiuc009gah.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ289242 mRNA. Translation: CAC10069.1.
BC013616 mRNA. Translation: AAH13616.1.
BC087554 mRNA. Translation: AAH87554.1.
CCDSiCCDS21061.1.
RefSeqiNP_068695.1. NM_021895.2.
UniGeneiMm.81144.

3D structure databases

ProteinModelPortaliP57780.
SMRiP57780. Positions 47-910.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208624. 10 interactions.
IntActiP57780. 16 interactions.
MINTiMINT-136628.
STRINGi10090.ENSMUSP00000066068.

PTM databases

iPTMnetiP57780.
PhosphoSiteiP57780.
SwissPalmiP57780.

Proteomic databases

EPDiP57780.
PaxDbiP57780.
PeptideAtlasiP57780.
PRIDEiP57780.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068045; ENSMUSP00000066068; ENSMUSG00000054808.
GeneIDi60595.
KEGGimmu:60595.
UCSCiuc009gah.1. mouse.

Organism-specific databases

CTDi81.
MGIiMGI:1890773. Actn4.

Phylogenomic databases

eggNOGiKOG0035. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000263418.
HOVERGENiHBG050453.
InParanoidiP57780.
KOiK05699.
OMAiIVGPWIQ.
OrthoDBiEOG72C4ZJ.
PhylomeDBiP57780.
TreeFamiTF352676.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Miscellaneous databases

ChiTaRSiActn4. mouse.
PROiP57780.
SOURCEiSearch...

Gene expression databases

BgeeiP57780.
CleanExiMM_ACTN4.
ExpressionAtlasiP57780. baseline and differential.
GenevisibleiP57780. MM.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.10.418.10. 2 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR029637. ACTN4.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR014837. EF-hand_Ca_insen.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PANTHERiPTHR11915:SF271. PTHR11915:SF271. 1 hit.
PfamiPF00307. CH. 2 hits.
PF08726. EFhand_Ca_insen. 1 hit.
PF00435. Spectrin. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00150. SPEC. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure, chromosomal localization and expression pattern of Capn12, a new member of the calpain large subunit gene family."
    Dear T.N., Meier N.T., Hunn M., Boehm T.
    Genomics 68:152-160(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 85-96 AND 311-319, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions."
    Nakatsuji H., Nishimura N., Yamamura R., Kanayama H.O., Sasaki T.
    Mol. Cell. Biol. 28:3324-3335(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TIGHT JUNCTION ASSEMBLY, INTERACTION WITH MICALL2, SUBCELLULAR LOCATION, REGION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-780 AND LYS-860, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiACTN4_MOUSE
AccessioniPrimary (citable) accession number: P57780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: July 6, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.