Alpha-actinin-4 - Mus musculus (Mouse)

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P57780 (ACTN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-actinin-4

Alternative name(s):
F-actin cross-linking protein
Non-muscle alpha-actinin 4

Gene names

Name:

Actn4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation By similarity.
Subunit structure	Homodimer; antiparallel By similarity. Interacts with BAIAP1 and PDLIM2 By similarity. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 By similarity. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Binds TRIM3 at the N-terminus.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers By similarity.
Sequence similarities	Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 2 EF-hand domains. Contains 4 spectrin repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	Actin-binding Calcium
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	actin filament bundle assembly Inferred from direct assay. Source: UniProtKB negative regulation of cellular component movement Inferred from direct assay. Source: UniProtKB positive regulation of cellular component movement Inferred from direct assay. Source: UniProtKB positive regulation of pinocytosis Inferred from mutant phenotype. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cortical cytoskeleton Inferred from direct assay. Source: MGI pseudopodium Inferred from direct assay. Source: UniProtKB ribonucleoprotein complex Inferred from sequence or structural similarity. Source: UniProtKB stress fiber Inferred from direct assay. Source: UniProtKB
Molecular function	actin filament binding Inferred from direct assay. Source: MGI calcium ion binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Micall2	Q3TN34	9	EBI-445071,EBI-1779852
Tjp1	P39447	6	EBI-445071,EBI-79508

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 912

912

Alpha-actinin-4

PRO_0000073441

Regions

Domain

1 – 267

267

Actin-binding

Domain

51 – 155

105

CH 1

Domain

164 – 267

104

CH 2

Repeat

294 – 404

111

Spectrin 1

Repeat

414 – 519

106

Spectrin 2

Repeat

529 – 640

112

Spectrin 3

Repeat

650 – 753

104

Spectrin 4

Domain

766 – 801

EF-hand 1

Domain

807 – 842

EF-hand 2

Calcium binding

779 – 790

1 Potential

Calcium binding

820 – 831

2 Potential

Region

178 – 193

Polyphosphoinositide (PIP2)-binding Potential

Amino acid modifications

Modified residue

115

N6-acetyllysine By similarity

Modified residue

215

N6-acetyllysine By similarity

Modified residue

266

Phosphotyrosine By similarity

Modified residue

418

N6-acetyllysine By similarity

Modified residue

593

N6-acetyllysine By similarity

Modified residue

626

N6-acetyllysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P57780 [UniParc].

Last modified February 21, 2001. Version 1.
Checksum: 07AA9C92AC228B5A
Show »

FASTA

912

104,977

        10         20         30         40         50         60 
MVDYHAANQA YQYGPNSGGG NGAGGGGSMG DYMAQEDDWD RDLLLDPAWE KQQRKTFTAW 

        70         80         90        100        110        120 
CNSHLRKAGT QIENIDEDFR DGLKLMLLLE VISGERLPKP ERGKMRVHKI NNVNKALDFI 

       130        140        150        160        170        180 
ASKGVKLVSI GAEEIVDGNA KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP 

       190        200        210        220        230        240 
YKNVNVQNFH ISWKDGLAFN ALIHRHRPEL IEYDKLRKDD PVTNLNNAFE VAEKYLDIPK 

       250        260        270        280        290        300 
MLDAEDIVNT ARPDEKAIMT YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEHLMEDYE 

       310        320        330        340        350        360 
RLASDLLEWI RRTIPWLEDR VPQKTIQEMQ QKLEDFRDYR RVHKPPKVQE KCQLEINFNT 

       370        380        390        400        410        420 
LQTKLRLSNR PAFMPSEGRM VSDINNGWQH LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR 

       430        440        450        460        470        480 
QKASIHEAWT DGKEAMLKQR DYETATLSDI KALIRKHEAF ESDLAAHQDR VEQIAAIAQE 

       490        500        510        520        530        540 
LNELDYYDSH NVNTRCQKIC DQWDNLGSLT HSRREALEKT EKQLETIDQL HLEYAKRAAP 

       550        560        570        580        590        600 
FNNWMESAME DLQDMFIVHT IEEIEGLISA HDQFKSTLPD ADREREAILA IHKEAQRIAE 

       610        620        630        640        650        660 
SNHIKLSGSN PYTTVTPQII NSKWEKVQQL VPKRDHALLE EQSKQQSNEH LRRQFASQAN 

       670        680        690        700        710        720 
MVGPWIQTKM EEIGRISIEM NGTLEDQLSH LKQYERSIVD YKPSLDLLEQ QHQLIQEALI 

       730        740        750        760        770        780 
FDNKHTNYTM EHIRVGWEQL LTTIARTINE VENQILTRDA KGISQEQMQE FRASFNHFDK 

       790        800        810        820        830        840 
DHGGALGPEE FKACLISLGY DVENDRQGDA EFNRIMSVVD PNHSGLVTFQ AFIDFMSRET 

       850        860        870        880        890        900 
TDTDTADQVI ASFKVLAGDK NFITAEELRR ELPPDQAEYC IARMAPYQGP DAAPGALDYK 

       910 
SFSTALYGES DL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene structure, chromosomal localization and expression pattern of Capn12, a new member of the calpain large subunit gene family." Dear T.N., Meier N.T., Hunn M., Boehm T. Genomics 68:152-160(2000) [PubMed: 10964513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 85-96 AND 311-319, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ289242 mRNA. Translation: CAC10069.1. BC013616 mRNA. Translation: AAH13616.1. BC087554 mRNA. Translation: AAH87554.1.
IPI	IPI00118899.
RefSeq	NP_068695.1. NM_021895.2.
UniGene	Mm.81144.
3D structure databases
ProteinModelPortal	P57780.
SMR	P57780. Positions 48-272, 287-912.
ModBase	Search...
Protein-protein interaction databases
IntAct	P57780. 10 interactions.
MINT	MINT-136628.
STRING	P57780.
PTM databases
PhosphoSite	P57780.
Proteomic databases
PRIDE	P57780.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000068045; ENSMUSP00000066068; ENSMUSG00000054808.
GeneID	60595.
KEGG	mmu:60595.
Organism-specific databases
CTD	81.
MGI	MGI:1890773. Actn4.
Phylogenomic databases
eggNOG	roNOG11088.
HOGENOM	HBG314462.
HOVERGEN	HBG050453.
InParanoid	P57780.
OMA	KPPRVEQ.
OrthoDB	EOG49P9XR.
Gene expression databases
ArrayExpress	P57780.
Bgee	P57780.
CleanEx	MM_ACTN4.
Genevestigator	P57780.
GermOnline	ENSMUSG00000054808. Mus musculus.
Family and domain databases
InterPro	IPR001589. Actinin_actin-bd_CS. IPR001715. CH-domain. IPR011992. EF-hand-like_dom. IPR014837. EF-hand_Ca_insen. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. IPR018159. Spectrin/alpha-actinin. IPR002017. Spectrin_repeat. [Graphical view]
Gene3D	G3DSA:1.10.418.10. Calponin-homology. 2 hits. G3DSA:1.10.238.10. EF-Hand_type. 2 hits.
KO	K05699.
Pfam	PF00307. CH. 2 hits. PF08726. efhand_Ca_insen. 1 hit. PF00435. Spectrin. 4 hits. [Graphical view]
SMART	SM00033. CH. 2 hits. SM00054. EFh. 2 hits. SM00150. SPEC. 4 hits. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit.
PROSITE	PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	315019.
SOURCE	Search...

Entry information

Entry name

ACTN4_MOUSE

Accession

Primary (citable) accession number: P57780

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	February 21, 2001
Last modified:	November 16, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents