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Protein

Alpha-actinin-4

Gene

Actn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (PubMed:18332111). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi779 – 7901PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi820 – 8312PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processProtein transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-actinin-4Curated
Alternative name(s):
Non-muscle alpha-actinin 4Curated
Gene namesi
Name:Actn4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1890773 Actn4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000734411 – 912Alpha-actinin-4Add BLAST912

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei250PhosphothreonineBy similarity1
Modified residuei593N6-acetyllysineBy similarity1
Modified residuei626N6-acetyllysineBy similarity1
Modified residuei780N6-acetyllysineCombined sources1
Modified residuei860N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP57780
PaxDbiP57780
PeptideAtlasiP57780
PRIDEiP57780

PTM databases

iPTMnetiP57780
PhosphoSitePlusiP57780
SwissPalmiP57780

Expressioni

Gene expression databases

BgeeiENSMUSG00000054808
CleanExiMM_ACTN4
ExpressionAtlasiP57780 baseline and differential
GenevisibleiP57780 MM

Interactioni

Subunit structurei

Homodimer; antiparallel (By similarity). Interacts with BAIAP1 and PDLIM2 (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Binds TRIM3 at the N-terminus. Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation. Interacts with PPARG and RARA (By similarity). Binds to VCL; this interaction triggers VCL conformational changes (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208624, 13 interactors
IntActiP57780, 17 interactors
MINTiP57780
STRINGi10090.ENSMUSP00000066068

Structurei

3D structure databases

ProteinModelPortaliP57780
SMRiP57780
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 155Calponin-homology (CH) 1PROSITE-ProRule annotationAdd BLAST105
Domaini164 – 270Calponin-homology (CH) 2PROSITE-ProRule annotationAdd BLAST107
Repeati294 – 404Spectrin 1Sequence analysisAdd BLAST111
Repeati414 – 519Spectrin 2Sequence analysisAdd BLAST106
Repeati529 – 640Spectrin 3Sequence analysisAdd BLAST112
Repeati650 – 753Spectrin 4Sequence analysisAdd BLAST104
Domaini766 – 801EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini807 – 842EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 267Actin-bindingAdd BLAST267
Regioni12 – 27Interaction with VCLBy similarityAdd BLAST16
Regioni41 – 62Interaction with VCLBy similarityAdd BLAST22
Regioni109 – 127Interaction with VCLBy similarityAdd BLAST19
Regioni178 – 193Polyphosphoinositide (PIP2)-bindingSequence analysisAdd BLAST16
Regioni737 – 912Mediates interaction with MICALL21 PublicationAdd BLAST176

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi85 – 89LXXLL motifBy similarity5

Domaini

Contains one Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif that mediates interaction with nuclear receptors.By similarity

Sequence similaritiesi

Belongs to the alpha-actinin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0035 Eukaryota
COG5069 LUCA
GeneTreeiENSGT00760000118813
HOGENOMiHOG000263418
HOVERGENiHBG050453
InParanoidiP57780
KOiK05699
OMAiQANIVGP
OrthoDBiEOG091G020R
PhylomeDBiP57780
TreeFamiTF352676

Family and domain databases

CDDicd00014 CH, 2 hits
cd00051 EFh, 1 hit
Gene3Di1.10.418.10, 2 hits
InterProiView protein in InterPro
IPR001589 Actinin_actin-bd_CS
IPR029637 ACTN4
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR011992 EF-hand-dom_pair
IPR014837 EF-hand_Ca_insen
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR018159 Spectrin/alpha-actinin
IPR002017 Spectrin_repeat
PANTHERiPTHR43947:SF7 PTHR43947:SF7, 1 hit
PfamiView protein in Pfam
PF00307 CH, 2 hits
PF08726 EFhand_Ca_insen, 1 hit
PF00435 Spectrin, 4 hits
SMARTiView protein in SMART
SM00033 CH, 2 hits
SM00054 EFh, 2 hits
SM00150 SPEC, 4 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF47576 SSF47576, 1 hit
PROSITEiView protein in PROSITE
PS00019 ACTININ_1, 1 hit
PS00020 ACTININ_2, 1 hit
PS50021 CH, 2 hits
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 2 hits

Sequencei

Sequence statusi: Complete.

P57780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDYHAANQA YQYGPNSGGG NGAGGGGSMG DYMAQEDDWD RDLLLDPAWE
60 70 80 90 100
KQQRKTFTAW CNSHLRKAGT QIENIDEDFR DGLKLMLLLE VISGERLPKP
110 120 130 140 150
ERGKMRVHKI NNVNKALDFI ASKGVKLVSI GAEEIVDGNA KMTLGMIWTI
160 170 180 190 200
ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNVQNFH ISWKDGLAFN
210 220 230 240 250
ALIHRHRPEL IEYDKLRKDD PVTNLNNAFE VAEKYLDIPK MLDAEDIVNT
260 270 280 290 300
ARPDEKAIMT YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEHLMEDYE
310 320 330 340 350
RLASDLLEWI RRTIPWLEDR VPQKTIQEMQ QKLEDFRDYR RVHKPPKVQE
360 370 380 390 400
KCQLEINFNT LQTKLRLSNR PAFMPSEGRM VSDINNGWQH LEQAEKGYEE
410 420 430 440 450
WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLKQR DYETATLSDI
460 470 480 490 500
KALIRKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSH NVNTRCQKIC
510 520 530 540 550
DQWDNLGSLT HSRREALEKT EKQLETIDQL HLEYAKRAAP FNNWMESAME
560 570 580 590 600
DLQDMFIVHT IEEIEGLISA HDQFKSTLPD ADREREAILA IHKEAQRIAE
610 620 630 640 650
SNHIKLSGSN PYTTVTPQII NSKWEKVQQL VPKRDHALLE EQSKQQSNEH
660 670 680 690 700
LRRQFASQAN MVGPWIQTKM EEIGRISIEM NGTLEDQLSH LKQYERSIVD
710 720 730 740 750
YKPSLDLLEQ QHQLIQEALI FDNKHTNYTM EHIRVGWEQL LTTIARTINE
760 770 780 790 800
VENQILTRDA KGISQEQMQE FRASFNHFDK DHGGALGPEE FKACLISLGY
810 820 830 840 850
DVENDRQGDA EFNRIMSVVD PNHSGLVTFQ AFIDFMSRET TDTDTADQVI
860 870 880 890 900
ASFKVLAGDK NFITAEELRR ELPPDQAEYC IARMAPYQGP DAAPGALDYK
910
SFSTALYGES DL
Length:912
Mass (Da):104,977
Last modified:February 21, 2001 - v1
Checksum:i07AA9C92AC228B5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ289242 mRNA Translation: CAC10069.1
BC013616 mRNA Translation: AAH13616.1
BC087554 mRNA Translation: AAH87554.1
CCDSiCCDS21061.1
RefSeqiNP_068695.1, NM_021895.2
UniGeneiMm.81144

Genome annotation databases

EnsembliENSMUST00000068045; ENSMUSP00000066068; ENSMUSG00000054808
GeneIDi60595
KEGGimmu:60595
UCSCiuc009gah.1 mouse

Similar proteinsi

Entry informationi

Entry nameiACTN4_MOUSE
AccessioniPrimary (citable) accession number: P57780
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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