Reviewed, UniProtKB/Swiss-Prot P57777 (HEMH_CAUCR)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	CC_3762

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Cofactor	Binds 1 2Fe-2S cluster. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subunit structure	Homodimer. HAMAP MF_00323
Subcellular location	Cytoplasm. HAMAP MF_00323
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Ferrochelatase HAMAP MF_00323		PRO_0000175126
Sites
	Metal binding	158	1	Iron-sulfur (2Fe-2S) HAMAP MF_00323
	Metal binding	193	1	Iron By similarity
	Metal binding	272	1	Iron By similarity
	Metal binding	332	1	Iron-sulfur (2Fe-2S) HAMAP MF_00323
	Metal binding	339	1	Iron-sulfur (2Fe-2S) HAMAP MF_00323
	Metal binding	341	1	Iron-sulfur (2Fe-2S) HAMAP MF_00323

Sequences

	Sequence		Length	Mass (Da)
	P57777-1 [UniParc]. Last modified February 21, 2001. Version 1. Checksum: E666EAFBF8269809	FASTA	347	37,121
	10 20 30 40 50 60 MTQKLAVVLF NLGGPDGPDA VRPFLFNLFR DPAIIGAPAL IRYPLAALIS TTREKSAKAN 70 80 90 100 110 120 YAIMGGGSPL LPETEKQARA LEAALALAMP GVEAKCFIAM RYWHPLTDET ARQVAAFAPD 130 140 150 160 170 180 QVVLLPLYPQ FSTTTTGSSL KAWKKTYKGS GVQTTVGCYP TEGGLIEAHA RMIRESWEKA 190 200 210 220 230 240 GSPTNIRLLF SAHGLPEKVI LAGDPYQKQV EATAAAVAAH LPPQIEWTVC YQSRVGPLKW 250 260 270 280 290 300 IGPSTDDEIR RAGGEDKGVM ITPIAFVSEH VETLVELDHE YAELAEEVGA APYLRVSALG 310 320 330 340 TAPEFIDGLA KAVRDSVGKA PGTVSSACGW RCGADWSKCP CREGASA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of [2Fe-2S] clusters in microbial ferrochelatases." Dailey T.A., Dailey H.A. J. Bacteriol. 184:2460-2464(2002) [PubMed: 11948160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION OF IRON-SULFUR CLUSTER.
[2]	"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF184071 Genomic DNA. Translation: AAG12242.1. AE005673 Genomic DNA. Translation: AAK25724.1.
PIR	H87715.
RefSeq	NP_422556.1.
3D structure databases
HSSP	HSSP built from PDB template 1HRK based on UniProtKB P22830.
ModBase	Search...
Genome annotation databases
GeneID	942450.
GenomeReviews	Gene locus CC_3762 in contig AE005673_GR.
KEGG	ccr:CC_3762.
NMPDR	fig|190650.1.peg.3736.
TIGR	CC_3762.
Phylogenomic databases
HOGENOM	P57777.
OMA	VKWLEPS.
Enzyme and pathway databases
BRENDA	4.99.1.1. 2191.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_CAUCR

Accession

Primary (citable) accession number: P57777

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	February 21, 2001
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)