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Protein

Elongation factor 1-delta

Gene

Eef1d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.By similarity
Isoform 3: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-delta
Short name:
EF-1-delta
Gene namesi
Name:Eef1d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913906. Eef1d.

Subcellular locationi

Isoform 3 :
  • Nucleus By similarity

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cytoplasm Source: UniProtKB
  • eukaryotic translation elongation factor 1 complex Source: InterPro
  • nucleolus Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001550472 – 281Elongation factor 1-deltaAdd BLAST280

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei17N6-acetyllysineBy similarity1
Modified residuei37PhosphoserineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei60PhosphoserineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei106PhosphoserineBy similarity1
Modified residuei107N6-acetyllysineBy similarity1
Modified residuei117N6-acetyllysine; alternateBy similarity1
Modified residuei117N6-succinyllysine; alternateCombined sources1
Modified residuei119PhosphoserineBy similarity1
Modified residuei129PhosphothreonineBy similarity1
Modified residuei133PhosphoserineCombined sources1
Modified residuei147PhosphothreonineCombined sources1
Modified residuei162Phosphoserine; by CK2Combined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP57776.
MaxQBiP57776.
PaxDbiP57776.
PeptideAtlasiP57776.
PRIDEiP57776.

2D gel databases

REPRODUCTION-2DPAGEP57776.

PTM databases

iPTMnetiP57776.
PhosphoSitePlusiP57776.
SwissPalmiP57776.

Expressioni

Gene expression databases

BgeeiENSMUSG00000055762.

Interactioni

Subunit structurei

EF-1 is composed of 4 subunits: alpha, beta, delta isoform 1, and gamma. Isoform 3 interacts with HSF1 and NFE2L2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211625. 2 interactors.
IntActiP57776. 6 interactors.
MINTiMINT-1856784.
STRINGi10090.ENSMUSP00000087110.

Structurei

3D structure databases

ProteinModelPortaliP57776.
SMRiP57776.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 115Leucine-zipperBy similarityAdd BLAST36
Regioni173 – 281Catalytic (GEF)By similarityAdd BLAST109

Sequence similaritiesi

Belongs to the EF-1-beta/EF-1-delta family.Curated

Phylogenomic databases

eggNOGiKOG1668. Eukaryota.
COG2092. LUCA.
HOGENOMiHOG000207272.
HOVERGENiHBG000787.
InParanoidiP57776.
KOiK15410.
TreeFamiTF313134.

Family and domain databases

CDDicd00292. EF1B. 1 hit.
Gene3Di3.30.70.60. 1 hit.
InterProiIPR018940. EF-1_beta_acid_region_euk.
IPR014038. EF1B_bsu/dsu_GNE.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
IPR001326. Transl_elong_EF1B_B/D_CS.
[Graphical view]
PfamiPF10587. EF-1_beta_acid. 1 hit.
PF00736. EF1_GNE. 1 hit.
[Graphical view]
SMARTiSM01182. EF-1_beta_acid. 1 hit.
SM00888. EF1_GNE. 1 hit.
[Graphical view]
SUPFAMiSSF54984. SSF54984. 1 hit.
PROSITEiPS00824. EF1BD_1. 1 hit.
PS00825. EF1BD_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P57776-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVTSGSRQE NGASVILRDI
60 70 80 90 100
ARARENIQKS LAGSSGPGAS SGPGGDHSEL IVRITSLEVE NQNLRGVVQD
110 120 130 140 150
LQQAISKLEA RLSSLEKSSP TPRATAPQTQ HVSPMRQVEP PTKKGATPAE
160 170 180 190 200
DDEDKDIDLF GSDEEEEDKE AARLREERLR QYAEKKAKKP TLVAKSSILL
210 220 230 240 250
DVKPWDDETD MAQLETCVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE
260 270 280
DDKVGTDLLE EEITKFEEHV QSVDIAAFDK I
Length:281
Mass (Da):31,293
Last modified:January 23, 2007 - v3
Checksum:iFD55F872FDA06819
GO
Isoform 2 (identifier: P57776-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     40-63: Missing.

Note: No experimental confirmation available.
Show »
Length:257
Mass (Da):28,729
Checksum:iE0072696BD8CAACF
GO
Isoform 3 (identifier: P57776-3) [UniParc]FASTAAdd to basket
Also known as: eEF1BdeltaLBy similarity

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRSGKASCAL...MSSLRPNRKM

Show »
Length:660
Mass (Da):72,931
Checksum:i0B4B8EBE385ADDA4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279D → N in BAB26870 (PubMed:16141072).Curated1
Sequence conflicti279D → N in BAB30841 (PubMed:16141072).Curated1
Sequence conflicti279D → N in AAH79855 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378851M → MRSGKASCALETVWEDRHKY EGAERRFHEQEATQVVAAAA TAAATASASVQQLLDEVPAV NGPSQEDTEDTEEAEAPNTS SRSDPGKSHECKKPIQKKRK RSPKSWLGQADLALVGLSAD HVWLDKPLFDQAESSYRQRL ADVAAQAAQPPALAPRGPCT HGSHVACHHVTWGIWVNKSC FDQAERAFVEWSQSLLLAAE GSHRQGTPDTGQQAVTPDLA LACQPCPPANGQPPLGSLQA LVREVWLEKPRYDAAERGFY EALFDGHPPGKVRLQERASQ AEGTRRGRRDHRSCNNVGNK RAGSKRANGEAPPAFPYWYF LHKDAEAPWLSKPTYDSAEC RHHAVEALRIAWRLEAASLA HRPTPRSGPSMSSLRPNRKM in isoform 3. 1 Publication1
Alternative sequenceiVSP_00135940 – 63Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF304351 mRNA. Translation: AAG17466.1.
AK010308 mRNA. No translation available.
AK010347 mRNA. Translation: BAB26870.1.
AK017616 mRNA. Translation: BAB30841.1.
AK017796 mRNA. No translation available.
BC079855 mRNA. Translation: AAH79855.1.
CCDSiCCDS27553.1. [P57776-3]
CCDS27554.1. [P57776-1]
CCDS70638.1. [P57776-2]
RefSeqiNP_001272358.1. NM_001285429.1.
NP_001272359.1. NM_001285430.1.
NP_001272360.1. NM_001285431.1.
NP_001272361.1. NM_001285432.1.
NP_001272362.1. NM_001285433.1.
NP_001272363.1. NM_001285434.1.
NP_075729.2. NM_023240.3.
NP_083939.1. NM_029663.2.
UniGeneiMm.258927.

Genome annotation databases

GeneIDi66656.
KEGGimmu:66656.
UCSCiuc007whj.2. mouse. [P57776-3]
uc007whm.2. mouse. [P57776-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF304351 mRNA. Translation: AAG17466.1.
AK010308 mRNA. No translation available.
AK010347 mRNA. Translation: BAB26870.1.
AK017616 mRNA. Translation: BAB30841.1.
AK017796 mRNA. No translation available.
BC079855 mRNA. Translation: AAH79855.1.
CCDSiCCDS27553.1. [P57776-3]
CCDS27554.1. [P57776-1]
CCDS70638.1. [P57776-2]
RefSeqiNP_001272358.1. NM_001285429.1.
NP_001272359.1. NM_001285430.1.
NP_001272360.1. NM_001285431.1.
NP_001272361.1. NM_001285432.1.
NP_001272362.1. NM_001285433.1.
NP_001272363.1. NM_001285434.1.
NP_075729.2. NM_023240.3.
NP_083939.1. NM_029663.2.
UniGeneiMm.258927.

3D structure databases

ProteinModelPortaliP57776.
SMRiP57776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211625. 2 interactors.
IntActiP57776. 6 interactors.
MINTiMINT-1856784.
STRINGi10090.ENSMUSP00000087110.

PTM databases

iPTMnetiP57776.
PhosphoSitePlusiP57776.
SwissPalmiP57776.

2D gel databases

REPRODUCTION-2DPAGEP57776.

Proteomic databases

EPDiP57776.
MaxQBiP57776.
PaxDbiP57776.
PeptideAtlasiP57776.
PRIDEiP57776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66656.
KEGGimmu:66656.
UCSCiuc007whj.2. mouse. [P57776-3]
uc007whm.2. mouse. [P57776-2]

Organism-specific databases

CTDi1936.
MGIiMGI:1913906. Eef1d.

Phylogenomic databases

eggNOGiKOG1668. Eukaryota.
COG2092. LUCA.
HOGENOMiHOG000207272.
HOVERGENiHBG000787.
InParanoidiP57776.
KOiK15410.
TreeFamiTF313134.

Miscellaneous databases

ChiTaRSiEef1d. mouse.
PROiP57776.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000055762.

Family and domain databases

CDDicd00292. EF1B. 1 hit.
Gene3Di3.30.70.60. 1 hit.
InterProiIPR018940. EF-1_beta_acid_region_euk.
IPR014038. EF1B_bsu/dsu_GNE.
IPR014717. Transl_elong_EF1B/ribosomal_S6.
IPR001326. Transl_elong_EF1B_B/D_CS.
[Graphical view]
PfamiPF10587. EF-1_beta_acid. 1 hit.
PF00736. EF1_GNE. 1 hit.
[Graphical view]
SMARTiSM01182. EF-1_beta_acid. 1 hit.
SM00888. EF1_GNE. 1 hit.
[Graphical view]
SUPFAMiSSF54984. SSF54984. 1 hit.
PROSITEiPS00824. EF1BD_1. 1 hit.
PS00825. EF1BD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF1D_MOUSE
AccessioniPrimary (citable) accession number: P57776
Secondary accession number(s): Q68FG5
, Q9CWW2, Q9CWY1, Q9CYD4, Q9CYJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.