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Protein

Selenocysteine-specific elongation factor

Gene

EEFSEC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTPSequence analysis8
Nucleotide bindingi92 – 96GTPSequence analysis5
Nucleotide bindingi146 – 150GTPSequence analysis5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2408557. Selenocysteine synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Selenocysteine-specific elongation factor
Alternative name(s):
Elongation factor sec
Eukaryotic elongation factor, selenocysteine-tRNA-specific
Gene namesi
Name:EEFSEC
Synonyms:SELB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24614. EEFSEC.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi60678.
OpenTargetsiENSG00000132394.
PharmGKBiPA142671916.

Polymorphism and mutation databases

BioMutaiEEFSEC.
DMDMi259016384.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000914781 – 596Selenocysteine-specific elongation factorAdd BLAST596

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei537PhosphoserineCombined sources1
Modified residuei545PhosphothreonineBy similarity1
Modified residuei556Omega-N-methylarginineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP57772.
MaxQBiP57772.
PaxDbiP57772.
PeptideAtlasiP57772.
PRIDEiP57772.

PTM databases

iPTMnetiP57772.
PhosphoSitePlusiP57772.

Expressioni

Gene expression databases

BgeeiENSG00000132394.
CleanExiHS_EEFSEC.
ExpressionAtlasiP57772. baseline and differential.
GenevisibleiP57772. HS.

Organism-specific databases

HPAiHPA035795.

Interactioni

Protein-protein interaction databases

BioGridi121953. 20 interactors.
IntActiP57772. 7 interactors.
STRINGi9606.ENSP00000254730.

Structurei

Secondary structure

1596
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 19Combined sources15
Helixi20 – 30Combined sources11
Beta strandi52 – 59Combined sources8
Helixi62 – 67Combined sources6
Beta strandi85 – 92Combined sources8
Beta strandi94 – 96Combined sources3
Helixi97 – 99Combined sources3
Helixi100 – 107Combined sources8
Beta strandi111 – 118Combined sources8
Turni119 – 121Combined sources3
Helixi125 – 137Combined sources13
Beta strandi139 – 146Combined sources8
Beta strandi149 – 151Combined sources3
Turni153 – 155Combined sources3
Helixi156 – 170Combined sources15
Beta strandi172 – 175Combined sources4
Beta strandi181 – 183Combined sources3
Beta strandi185 – 187Combined sources3
Helixi202 – 212Combined sources11
Beta strandi225 – 233Combined sources9
Beta strandi235 – 237Combined sources3
Beta strandi239 – 251Combined sources13
Beta strandi255 – 257Combined sources3
Turni259 – 261Combined sources3
Beta strandi264 – 272Combined sources9
Beta strandi273 – 275Combined sources3
Beta strandi278 – 281Combined sources4
Beta strandi285 – 291Combined sources7
Helixi295 – 297Combined sources3
Beta strandi299 – 305Combined sources7
Beta strandi309 – 317Combined sources9
Beta strandi319 – 321Combined sources3
Beta strandi330 – 332Combined sources3
Beta strandi334 – 339Combined sources6
Beta strandi342 – 347Combined sources6
Beta strandi350 – 353Combined sources4
Helixi355 – 357Combined sources3
Beta strandi358 – 360Combined sources3
Beta strandi370 – 378Combined sources9
Beta strandi410 – 422Combined sources13
Beta strandi427 – 431Combined sources5
Beta strandi444 – 454Combined sources11
Helixi459 – 462Combined sources4
Helixi464 – 466Combined sources3
Beta strandi468 – 481Combined sources14
Beta strandi483 – 492Combined sources10
Helixi500 – 503Combined sources4
Beta strandi507 – 510Combined sources4
Turni511 – 513Combined sources3
Beta strandi515 – 518Combined sources4
Helixi538 – 542Combined sources5
Beta strandi575 – 584Combined sources10
Helixi586 – 588Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IZKX-ray3.25A/B1-596[»]
5IZLX-ray2.72A/B1-596[»]
5IZMX-ray3.40A/B2-596[»]
ProteinModelPortaliP57772.
SMRiP57772.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 217tr-type GPROSITE-ProRule annotationAdd BLAST213

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 21G1PROSITE-ProRule annotation8
Regioni46 – 50G2PROSITE-ProRule annotation5
Regioni92 – 95G3PROSITE-ProRule annotation4
Regioni146 – 149G4PROSITE-ProRule annotation4
Regioni185 – 187G5PROSITE-ProRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi547 – 553Nuclear localization signalSequence analysis7

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. SelB subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0461. Eukaryota.
COG3276. LUCA.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000247007.
HOVERGENiHBG017788.
InParanoidiP57772.
KOiK03833.
OMAiGQGTVCT.
OrthoDBiEOG090B04EU.
PhylomeDBiP57772.
TreeFamiTF300432.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51722. G_TR_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P57772-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRRVNVNV GVLGHIDSGK TALARALSTT ASTAAFDKQP QSRERGITLD
60 70 80 90 100
LGFSCFSVPL PARLRSSLPE FQAAPEAEPE PGEPLLQVTL VDCPGHASLI
110 120 130 140 150
RTIIGGAQII DLMMLVIDVT KGMQTQSAEC LVIGQIACQK LVVVLNKIDL
160 170 180 190 200
LPEGKRQAAI DKMTKKMQKT LENTKFRGAP IIPVAAKPGG PEAPETEAPQ
210 220 230 240 250
GIPELIELLT SQISIPTRDP SGPFLMSVDH CFSIKGQGTV MTGTILSGSI
260 270 280 290 300
SLGDSVEIPA LKVVKKVKSM QMFHMPITSA MQGDRLGICV TQFDPKLLER
310 320 330 340 350
GLVCAPESLH TVHAALISVE KIPYFRGPLQ TKAKFHITVG HETVMGRLMF
360 370 380 390 400
FSPAPDNFDQ EPILDSFNFS QEYLFQEQYL SKDLTPAVTD NDEADKKAGQ
410 420 430 440 450
ATEGHCPRQQ WALVEFEKPV TCPRLCLVIG SRLDADIHTN TCRLAFHGIL
460 470 480 490 500
LHGLEDRNYA DSFLPRLKVY KLKHKHGLVE RAMDDYSVIG RSLFKKETNI
510 520 530 540 550
QLFVGLKVHL STGELGIIDS AFGQSGKFKI HIPGGLSPES KKILTPALKK
560 570 580 590
RARAGRGEAT RQEESAERSE PSQHVVLSLT FKRYVFDTHK RMVQSP
Length:596
Mass (Da):65,305
Last modified:September 22, 2009 - v4
Checksum:iC92C43E3F250F686
GO
Isoform 2 (identifier: P57772-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: MAGRRVNVNVGVLGHIDSGKTALARALSTTA → MASC

Note: No experimental confirmation available.
Show »
Length:569
Mass (Da):62,579
Checksum:i8AC15AC26DB4F2B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti577L → F in AAG13375 (PubMed:10970870).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055712435A → V. Corresponds to variant dbSNP:rs34326479Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0571851 – 31MAGRR…LSTTA → MASC in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL449214 Genomic DNA. No translation available.
AL449210 Genomic DNA. No translation available.
AL449217 Genomic DNA. No translation available.
BC007933 mRNA. Translation: AAH07933.1.
AF268872 mRNA. Translation: AAG13375.1.
CCDSiCCDS33849.1. [P57772-1]
RefSeqiNP_068756.2. NM_021937.4. [P57772-1]
UniGeneiHs.477498.

Genome annotation databases

EnsembliENST00000254730; ENSP00000254730; ENSG00000132394. [P57772-1]
GeneIDi60678.
KEGGihsa:60678.
UCSCiuc003eki.4. human. [P57772-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL449214 Genomic DNA. No translation available.
AL449210 Genomic DNA. No translation available.
AL449217 Genomic DNA. No translation available.
BC007933 mRNA. Translation: AAH07933.1.
AF268872 mRNA. Translation: AAG13375.1.
CCDSiCCDS33849.1. [P57772-1]
RefSeqiNP_068756.2. NM_021937.4. [P57772-1]
UniGeneiHs.477498.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IZKX-ray3.25A/B1-596[»]
5IZLX-ray2.72A/B1-596[»]
5IZMX-ray3.40A/B2-596[»]
ProteinModelPortaliP57772.
SMRiP57772.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121953. 20 interactors.
IntActiP57772. 7 interactors.
STRINGi9606.ENSP00000254730.

PTM databases

iPTMnetiP57772.
PhosphoSitePlusiP57772.

Polymorphism and mutation databases

BioMutaiEEFSEC.
DMDMi259016384.

Proteomic databases

EPDiP57772.
MaxQBiP57772.
PaxDbiP57772.
PeptideAtlasiP57772.
PRIDEiP57772.

Protocols and materials databases

DNASUi60678.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254730; ENSP00000254730; ENSG00000132394. [P57772-1]
GeneIDi60678.
KEGGihsa:60678.
UCSCiuc003eki.4. human. [P57772-1]

Organism-specific databases

CTDi60678.
DisGeNETi60678.
GeneCardsiEEFSEC.
H-InvDBHIX0003649.
HGNCiHGNC:24614. EEFSEC.
HPAiHPA035795.
MIMi607695. gene.
neXtProtiNX_P57772.
OpenTargetsiENSG00000132394.
PharmGKBiPA142671916.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0461. Eukaryota.
COG3276. LUCA.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000247007.
HOVERGENiHBG017788.
InParanoidiP57772.
KOiK03833.
OMAiGQGTVCT.
OrthoDBiEOG090B04EU.
PhylomeDBiP57772.
TreeFamiTF300432.

Enzyme and pathway databases

ReactomeiR-HSA-2408557. Selenocysteine synthesis.

Miscellaneous databases

GenomeRNAii60678.
PROiP57772.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132394.
CleanExiHS_EEFSEC.
ExpressionAtlasiP57772. baseline and differential.
GenevisibleiP57772. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51722. G_TR_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSELB_HUMAN
AccessioniPrimary (citable) accession number: P57772
Secondary accession number(s): Q96HZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: September 22, 2009
Last modified: February 15, 2017
This is version 135 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.