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Protein

Selenocysteine-specific elongation factor

Gene

EEFSEC

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPSequence analysis
Nucleotide bindingi92 – 965GTPSequence analysis
Nucleotide bindingi146 – 1505GTPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2408557. Selenocysteine synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Selenocysteine-specific elongation factor
Alternative name(s):
Elongation factor sec
Eukaryotic elongation factor, selenocysteine-tRNA-specific
Gene namesi
Name:EEFSEC
Synonyms:SELB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:24614. EEFSEC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671916.

Polymorphism and mutation databases

BioMutaiEEFSEC.
DMDMi259016384.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 596596Selenocysteine-specific elongation factorPRO_0000091478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei537 – 5371PhosphoserineCombined sources
Modified residuei545 – 5451PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP57772.
MaxQBiP57772.
PaxDbiP57772.
PeptideAtlasiP57772.
PRIDEiP57772.

PTM databases

iPTMnetiP57772.
PhosphoSiteiP57772.

Expressioni

Gene expression databases

BgeeiENSG00000132394.
CleanExiHS_EEFSEC.
ExpressionAtlasiP57772. baseline and differential.
GenevisibleiP57772. HS.

Organism-specific databases

HPAiHPA035795.

Interactioni

Protein-protein interaction databases

BioGridi121953. 20 interactions.
IntActiP57772. 7 interactions.
STRINGi9606.ENSP00000254730.

Structurei

3D structure databases

ProteinModelPortaliP57772.
SMRiP57772. Positions 7-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 217213tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 218G1PROSITE-ProRule annotation
Regioni46 – 505G2PROSITE-ProRule annotation
Regioni92 – 954G3PROSITE-ProRule annotation
Regioni146 – 1494G4PROSITE-ProRule annotation
Regioni185 – 1873G5PROSITE-ProRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi547 – 5537Nuclear localization signalSequence analysis

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. SelB subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0461. Eukaryota.
COG3276. LUCA.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000247007.
HOVERGENiHBG017788.
InParanoidiP57772.
KOiK03833.
OMAiGQGTVCT.
OrthoDBiEOG091G0AQO.
PhylomeDBiP57772.
TreeFamiTF300432.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P57772-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRRVNVNV GVLGHIDSGK TALARALSTT ASTAAFDKQP QSRERGITLD
60 70 80 90 100
LGFSCFSVPL PARLRSSLPE FQAAPEAEPE PGEPLLQVTL VDCPGHASLI
110 120 130 140 150
RTIIGGAQII DLMMLVIDVT KGMQTQSAEC LVIGQIACQK LVVVLNKIDL
160 170 180 190 200
LPEGKRQAAI DKMTKKMQKT LENTKFRGAP IIPVAAKPGG PEAPETEAPQ
210 220 230 240 250
GIPELIELLT SQISIPTRDP SGPFLMSVDH CFSIKGQGTV MTGTILSGSI
260 270 280 290 300
SLGDSVEIPA LKVVKKVKSM QMFHMPITSA MQGDRLGICV TQFDPKLLER
310 320 330 340 350
GLVCAPESLH TVHAALISVE KIPYFRGPLQ TKAKFHITVG HETVMGRLMF
360 370 380 390 400
FSPAPDNFDQ EPILDSFNFS QEYLFQEQYL SKDLTPAVTD NDEADKKAGQ
410 420 430 440 450
ATEGHCPRQQ WALVEFEKPV TCPRLCLVIG SRLDADIHTN TCRLAFHGIL
460 470 480 490 500
LHGLEDRNYA DSFLPRLKVY KLKHKHGLVE RAMDDYSVIG RSLFKKETNI
510 520 530 540 550
QLFVGLKVHL STGELGIIDS AFGQSGKFKI HIPGGLSPES KKILTPALKK
560 570 580 590
RARAGRGEAT RQEESAERSE PSQHVVLSLT FKRYVFDTHK RMVQSP
Length:596
Mass (Da):65,305
Last modified:September 22, 2009 - v4
Checksum:iC92C43E3F250F686
GO
Isoform 2 (identifier: P57772-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: MAGRRVNVNVGVLGHIDSGKTALARALSTTA → MASC

Note: No experimental confirmation available.
Show »
Length:569
Mass (Da):62,579
Checksum:i8AC15AC26DB4F2B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti577 – 5771L → F in AAG13375 (PubMed:10970870).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti435 – 4351A → V.
Corresponds to variant rs34326479 [ dbSNP | Ensembl ].
VAR_055712

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3131MAGRR…LSTTA → MASC in isoform 2. 1 PublicationVSP_057185Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL449214 Genomic DNA. No translation available.
AL449210 Genomic DNA. No translation available.
AL449217 Genomic DNA. No translation available.
BC007933 mRNA. Translation: AAH07933.1.
AF268872 mRNA. Translation: AAG13375.1.
CCDSiCCDS33849.1. [P57772-1]
RefSeqiNP_068756.2. NM_021937.4. [P57772-1]
UniGeneiHs.477498.

Genome annotation databases

EnsembliENST00000254730; ENSP00000254730; ENSG00000132394. [P57772-1]
GeneIDi60678.
KEGGihsa:60678.
UCSCiuc003eki.4. human. [P57772-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL449214 Genomic DNA. No translation available.
AL449210 Genomic DNA. No translation available.
AL449217 Genomic DNA. No translation available.
BC007933 mRNA. Translation: AAH07933.1.
AF268872 mRNA. Translation: AAG13375.1.
CCDSiCCDS33849.1. [P57772-1]
RefSeqiNP_068756.2. NM_021937.4. [P57772-1]
UniGeneiHs.477498.

3D structure databases

ProteinModelPortaliP57772.
SMRiP57772. Positions 7-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121953. 20 interactions.
IntActiP57772. 7 interactions.
STRINGi9606.ENSP00000254730.

PTM databases

iPTMnetiP57772.
PhosphoSiteiP57772.

Polymorphism and mutation databases

BioMutaiEEFSEC.
DMDMi259016384.

Proteomic databases

EPDiP57772.
MaxQBiP57772.
PaxDbiP57772.
PeptideAtlasiP57772.
PRIDEiP57772.

Protocols and materials databases

DNASUi60678.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254730; ENSP00000254730; ENSG00000132394. [P57772-1]
GeneIDi60678.
KEGGihsa:60678.
UCSCiuc003eki.4. human. [P57772-1]

Organism-specific databases

CTDi60678.
GeneCardsiEEFSEC.
H-InvDBHIX0003649.
HGNCiHGNC:24614. EEFSEC.
HPAiHPA035795.
MIMi607695. gene.
neXtProtiNX_P57772.
PharmGKBiPA142671916.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0461. Eukaryota.
COG3276. LUCA.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000247007.
HOVERGENiHBG017788.
InParanoidiP57772.
KOiK03833.
OMAiGQGTVCT.
OrthoDBiEOG091G0AQO.
PhylomeDBiP57772.
TreeFamiTF300432.

Enzyme and pathway databases

ReactomeiR-HSA-2408557. Selenocysteine synthesis.

Miscellaneous databases

GenomeRNAii60678.
PROiP57772.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132394.
CleanExiHS_EEFSEC.
ExpressionAtlasiP57772. baseline and differential.
GenevisibleiP57772. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSELB_HUMAN
AccessioniPrimary (citable) accession number: P57772
Secondary accession number(s): Q96HZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: September 22, 2009
Last modified: September 7, 2016
This is version 130 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.