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Protein

Regulator of G-protein signaling 8

Gene

RGS8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades, including signaling via muscarinic acetylcholine receptor CHRM2 and dopamine receptor DRD2 (By similarity). Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:18434541). Modulates the activity of potassium channels that are activated in response to DRD2 and CHRM2 signaling (By similarity).By similarity1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Signal transduction inhibitor

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135824-MONOMER.
ReactomeiR-HSA-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 8
Short name:
RGS8
Gene namesi
Name:RGS8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:16810. RGS8.

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Perikaryon By similarity
  • Cell projectiondendrite By similarity
  • Nucleus By similarity

  • Note: Detected in Purkinje cell soma and dendrites. Associated with Purkinje cell membranes. Not detected in Purkinje cell nuclei. Detected in the nucleus after heterologous expression. Recruited to the cell membrane in the presence of GNAO1.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi85397.
OpenTargetsiENSG00000135824.
PharmGKBiPA34379.

Chemistry databases

ChEMBLiCHEMBL2034803.
GuidetoPHARMACOLOGYi2813.

Polymorphism and mutation databases

BioMutaiRGS8.
DMDMi13124465.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041991 – 180Regulator of G-protein signaling 8Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP57771.
PeptideAtlasiP57771.
PRIDEiP57771.

PTM databases

PhosphoSitePlusiP57771.

Expressioni

Gene expression databases

BgeeiENSG00000135824.
CleanExiHS_RGS8.
ExpressionAtlasiP57771. baseline and differential.
GenevisibleiP57771. HS.

Interactioni

Subunit structurei

Interacts with GNAO1 (By similarity). Interacts with GNAI3.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GOLGA2Q083793EBI-10216117,EBI-618309

Protein-protein interaction databases

BioGridi124512. 4 interactors.
DIPiDIP-59093N.
IntActiP57771. 6 interactors.
STRINGi9606.ENSP00000258302.

Chemistry databases

BindingDBiP57771.

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi47 – 52Combined sources6
Turni53 – 55Combined sources3
Helixi57 – 61Combined sources5
Helixi64 – 76Combined sources13
Helixi81 – 92Combined sources12
Helixi98 – 112Combined sources15
Helixi125 – 134Combined sources10
Turni140 – 143Combined sources4
Helixi144 – 156Combined sources13
Helixi158 – 163Combined sources6
Helixi166 – 169Combined sources4
Turni170 – 172Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IHDX-ray1.70A42-173[»]
2ODEX-ray1.90B/D42-180[»]
5DO9X-ray2.60B/D/F42-173[»]
ProteinModelPortaliP57771.
SMRiP57771.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP57771.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 171RGSPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP57771.
KOiK16449.
OMAiSHKSDSY.
OrthoDBiEOG091G0BDG.
PhylomeDBiP57771.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P57771-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALLMPRRN KGMRTRLGCL SHKSDSCSDF TAILPDKPNR ALKRLSTEEA
60 70 80 90 100
TRWADSFDVL LSHKYGVAAF RAFLKTEFSE ENLEFWLACE EFKKTRSTAK
110 120 130 140 150
LVSKAHRIFE EFVDVQAPRE VNIDFQTREA TRKNLQEPSL TCFDQAQGKV
160 170 180
HSLMEKDSYP RFLRSKMYLD LLSQSQRRLS
Length:180
Mass (Da):20,917
Last modified:February 21, 2001 - v1
Checksum:i198965B4C27F64C9
GO
Isoform 2 (identifier: P57771-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MAALLMPRR → MWNTLTRSLSDHPVGKDPQAMRTGQRQ

Show »
Length:198
Mass (Da):22,970
Checksum:i2A167BCE750725E5
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0364211 – 9MAALLMPRR → MWNTLTRSLSDHPVGKDPQA MRTGQRQ in isoform 2. 2 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297015 mRNA. Translation: AAG45337.1.
AF300649 mRNA. Translation: AAG18443.1.
AK296514 mRNA. Translation: BAG59146.1.
AK294660 mRNA. Translation: BAG57830.1.
AL353778 Genomic DNA. Translation: CAH72421.1.
CH471067 Genomic DNA. Translation: EAW91130.1.
BC069677 mRNA. Translation: AAH69677.1.
BC069718 mRNA. Translation: AAH69718.1.
BC103865 mRNA. Translation: AAI03866.1.
BC103866 mRNA. Translation: AAI03867.1.
BC103867 mRNA. Translation: AAI03868.1.
CCDSiCCDS1349.1. [P57771-2]
CCDS41443.1. [P57771-1]
RefSeqiNP_001095920.1. NM_001102450.2. [P57771-1]
NP_203131.1. NM_033345.3. [P57771-2]
XP_005245612.1. XM_005245555.3. [P57771-1]
XP_011508391.1. XM_011510089.2. [P57771-2]
XP_011508392.1. XM_011510090.2. [P57771-1]
XP_016858120.1. XM_017002631.1. [P57771-2]
XP_016858121.1. XM_017002632.1. [P57771-2]
XP_016858122.1. XM_017002633.1. [P57771-2]
XP_016858123.1. XM_017002634.1. [P57771-2]
XP_016858124.1. XM_017002635.1. [P57771-1]
XP_016858125.1. XM_017002636.1. [P57771-1]
XP_016858126.1. XM_017002637.1. [P57771-1]
UniGeneiHs.20982.
Hs.458417.

Genome annotation databases

EnsembliENST00000258302; ENSP00000258302; ENSG00000135824. [P57771-2]
ENST00000367556; ENSP00000356527; ENSG00000135824. [P57771-1]
ENST00000367557; ENSP00000356528; ENSG00000135824. [P57771-1]
ENST00000483095; ENSP00000426289; ENSG00000135824. [P57771-1]
GeneIDi85397.
KEGGihsa:85397.
UCSCiuc001gpm.2. human. [P57771-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297015 mRNA. Translation: AAG45337.1.
AF300649 mRNA. Translation: AAG18443.1.
AK296514 mRNA. Translation: BAG59146.1.
AK294660 mRNA. Translation: BAG57830.1.
AL353778 Genomic DNA. Translation: CAH72421.1.
CH471067 Genomic DNA. Translation: EAW91130.1.
BC069677 mRNA. Translation: AAH69677.1.
BC069718 mRNA. Translation: AAH69718.1.
BC103865 mRNA. Translation: AAI03866.1.
BC103866 mRNA. Translation: AAI03867.1.
BC103867 mRNA. Translation: AAI03868.1.
CCDSiCCDS1349.1. [P57771-2]
CCDS41443.1. [P57771-1]
RefSeqiNP_001095920.1. NM_001102450.2. [P57771-1]
NP_203131.1. NM_033345.3. [P57771-2]
XP_005245612.1. XM_005245555.3. [P57771-1]
XP_011508391.1. XM_011510089.2. [P57771-2]
XP_011508392.1. XM_011510090.2. [P57771-1]
XP_016858120.1. XM_017002631.1. [P57771-2]
XP_016858121.1. XM_017002632.1. [P57771-2]
XP_016858122.1. XM_017002633.1. [P57771-2]
XP_016858123.1. XM_017002634.1. [P57771-2]
XP_016858124.1. XM_017002635.1. [P57771-1]
XP_016858125.1. XM_017002636.1. [P57771-1]
XP_016858126.1. XM_017002637.1. [P57771-1]
UniGeneiHs.20982.
Hs.458417.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IHDX-ray1.70A42-173[»]
2ODEX-ray1.90B/D42-180[»]
5DO9X-ray2.60B/D/F42-173[»]
ProteinModelPortaliP57771.
SMRiP57771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124512. 4 interactors.
DIPiDIP-59093N.
IntActiP57771. 6 interactors.
STRINGi9606.ENSP00000258302.

Chemistry databases

BindingDBiP57771.
ChEMBLiCHEMBL2034803.
GuidetoPHARMACOLOGYi2813.

PTM databases

PhosphoSitePlusiP57771.

Polymorphism and mutation databases

BioMutaiRGS8.
DMDMi13124465.

Proteomic databases

PaxDbiP57771.
PeptideAtlasiP57771.
PRIDEiP57771.

Protocols and materials databases

DNASUi85397.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000258302; ENSP00000258302; ENSG00000135824. [P57771-2]
ENST00000367556; ENSP00000356527; ENSG00000135824. [P57771-1]
ENST00000367557; ENSP00000356528; ENSG00000135824. [P57771-1]
ENST00000483095; ENSP00000426289; ENSG00000135824. [P57771-1]
GeneIDi85397.
KEGGihsa:85397.
UCSCiuc001gpm.2. human. [P57771-1]

Organism-specific databases

CTDi85397.
DisGeNETi85397.
GeneCardsiRGS8.
HGNCiHGNC:16810. RGS8.
MIMi607189. gene.
neXtProtiNX_P57771.
OpenTargetsiENSG00000135824.
PharmGKBiPA34379.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP57771.
KOiK16449.
OMAiSHKSDSY.
OrthoDBiEOG091G0BDG.
PhylomeDBiP57771.
TreeFamiTF315837.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135824-MONOMER.
ReactomeiR-HSA-418594. G alpha (i) signalling events.

Miscellaneous databases

EvolutionaryTraceiP57771.
GeneWikiiRGS8.
GenomeRNAii85397.
PROiP57771.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135824.
CleanExiHS_RGS8.
ExpressionAtlasiP57771. baseline and differential.
GenevisibleiP57771. HS.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS8_HUMAN
AccessioniPrimary (citable) accession number: P57771
Secondary accession number(s): B4DGL9, Q3SYD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.