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Protein

DNA polymerase sliding clamp 1

Gene

pcn1

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the sliding clamp subunits that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication. Heterotrimer stimulates the Holliday junction resolvase Hjc. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase sliding clamp 1UniRule annotation
Alternative name(s):
Proliferating cell nuclear antigen homolog 1UniRule annotation
Short name:
PCNA1UniRule annotation
Gene namesi
Name:pcn1UniRule annotation
Synonyms:pcnA-like
Ordered Locus Names:SSO0397
ORF Names:C41_008
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114 – 116YIK → ELE: Loss of interaction with PCNA3, no change with PCNA2. 1 Publication3
Mutagenesisi175 – 177KRY → EED: Loss of interaction with both PCNA3 and PCNA2. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001492151 – 249DNA polymerase sliding clamp 1Add BLAST249

Interactioni

Subunit structurei

Forms heterodimers with PCNA2, which then recruit PCNA3; does not form homotrimers (PubMed:12535540, PubMed:18703842). The heterodimers interact with RfcS homotetramers (PubMed:12535540). Heterotrimer which circularizes head-to-tail (head is at N-terminus, tail is at C-terminus) to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA. Heterotrimer interacts, probably via this subunit, with flap endonuclease 1 (fen) (PubMed:12535540), Hjc (PubMed:17011573), Dpo4 (PubMed:19054331), and XPF (PubMed:12675797).8 Publications

Protein-protein interaction databases

DIPiDIP-48854N.
STRINGi273057.SSO0397.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi9 – 19Combined sources11
Turni20 – 22Combined sources3
Beta strandi24 – 30Combined sources7
Beta strandi32 – 40Combined sources9
Beta strandi44 – 53Combined sources10
Helixi54 – 56Combined sources3
Beta strandi57 – 61Combined sources5
Beta strandi66 – 70Combined sources5
Helixi72 – 79Combined sources8
Beta strandi87 – 93Combined sources7
Beta strandi95 – 104Combined sources10
Turni105 – 107Combined sources3
Beta strandi110 – 116Combined sources7
Beta strandi118 – 120Combined sources3
Beta strandi134 – 138Combined sources5
Helixi140 – 153Combined sources14
Beta strandi155 – 162Combined sources8
Beta strandi165 – 172Combined sources8
Beta strandi175 – 183Combined sources9
Beta strandi185 – 194Combined sources10
Beta strandi197 – 201Combined sources5
Helixi202 – 210Combined sources9
Turni211 – 214Combined sources4
Beta strandi219 – 223Combined sources5
Beta strandi229 – 234Combined sources6
Beta strandi240 – 245Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HIIX-ray2.79A/X1-249[»]
2HIKX-ray3.30A/L/X1-249[»]
2IO4X-ray2.60A/C1-249[»]
2IX2X-ray2.20A1-249[»]
2IZOX-ray2.90C1-249[»]
2NTIX-ray2.50A/D/G1-249[»]
3FDSX-ray2.05C1-249[»]
ProteinModelPortaliP57766.
SMRiP57766.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP57766.

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000270356.
InParanoidiP57766.
KOiK04802.
OMAiKRGTRND.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF00705. PCNA_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57766-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKIVYPNAK DFFSFINSIT NVTDSIILNF TEDGIFSRHL TEDKVLMAIM
60 70 80 90 100
RIPKDVLSEY SIDSPTSVKL DVSSVKKILS KASSKKATIE LTETDSGLKI
110 120 130 140 150
IIRDEKSGAK STIYIKAEKG QVEQLTEPKV NLAVNFTTDE SVLNVIAADV
160 170 180 190 200
TLVGEEMRIS TEEDKIKIEA GEEGKRYVAF LMKDKPLKEL SIDTSASSSY
210 220 230 240
SAEMFKDAVK GLRGFSAPTM VSFGENLPMK IDVEAVSGGH MIFWIAPRL
Length:249
Mass (Da):27,536
Last modified:February 21, 2001 - v1
Checksum:i137EB10BE2C03F77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40726.1.
PIRiG90183.
RefSeqiWP_009988793.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40726; AAK40726; SSO0397.
GeneIDi27426696.
KEGGisso:SSO0397.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006641 Genomic DNA. Translation: AAK40726.1.
PIRiG90183.
RefSeqiWP_009988793.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HIIX-ray2.79A/X1-249[»]
2HIKX-ray3.30A/L/X1-249[»]
2IO4X-ray2.60A/C1-249[»]
2IX2X-ray2.20A1-249[»]
2IZOX-ray2.90C1-249[»]
2NTIX-ray2.50A/D/G1-249[»]
3FDSX-ray2.05C1-249[»]
ProteinModelPortaliP57766.
SMRiP57766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48854N.
STRINGi273057.SSO0397.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40726; AAK40726; SSO0397.
GeneIDi27426696.
KEGGisso:SSO0397.

Phylogenomic databases

eggNOGiarCOG00488. Archaea.
COG0592. LUCA.
HOGENOMiHOG000270356.
InParanoidiP57766.
KOiK04802.
OMAiKRGTRND.

Miscellaneous databases

EvolutionaryTraceiP57766.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF00705. PCNA_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCNA1_SULSO
AccessioniPrimary (citable) accession number: P57766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.