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P57760

- STK16_RAT

UniProt

P57760 - STK16_RAT

Protein

Serine/threonine-protein kinase 16

Gene

Stk16

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates By similarity. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotation
    Active sitei148 – 1481Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein kinase activity Source: RGD
    4. protein serine/threonine kinase activity Source: UniProtKB-KW
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: RGD

    GO - Biological processi

    1. cellular response to transforming growth factor beta stimulus Source: RGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: RGD
    3. protein autophosphorylation Source: RGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase 16 (EC:2.7.11.1)
    Alternative name(s):
    Myristoylated and palmitoylated serine/threonine-protein kinase
    Short name:
    MPSK
    Protein kinase PKL12
    TGF-beta-stimulated factor 1
    Short name:
    TSF-1
    Tyrosine-protein kinase STK16 (EC:2.7.10.2)
    Gene namesi
    Name:Stk16
    Synonyms:Mpsk1, Tsf1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi629474. Stk16.

    Subcellular locationi

    Cytoplasmperinuclear region. Membrane By similarity; Lipid-anchor By similarity
    Note: Associates with Golgi and Golgi-derived vesicles.By similarity

    GO - Cellular componenti

    1. Golgi-associated vesicle Source: Ensembl
    2. membrane Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 305304Serine/threonine-protein kinase 16PRO_0000086703Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi6 – 61S-palmitoyl cysteineBy similarity
    Lipidationi8 – 81S-palmitoyl cysteineBy similarity
    Modified residuei197 – 1971Phosphoserine; by autocatalysisBy similarity
    Modified residuei198 – 1981Phosphotyrosine; by autocatalysisBy similarity

    Post-translational modificationi

    Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198 By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP57760.

    PTM databases

    PhosphoSiteiP57760.

    Expressioni

    Tissue specificityi

    Expressed in heart, liver, brain, spleen, lung, skeletal muscle, kidney and testis.1 Publication

    Inductioni

    By TGF-beta.1 Publication

    Gene expression databases

    GenevestigatoriP57760.

    Interactioni

    Subunit structurei

    Monomer. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1.By similarity

    Protein-protein interaction databases

    MINTiMINT-4570539.
    STRINGi10116.ENSRNOP00000026194.

    Structurei

    3D structure databases

    ProteinModelPortaliP57760.
    SMRiP57760. Positions 13-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 293274Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 20237Activation loopBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000075037.
    HOGENOMiHOG000157694.
    HOVERGENiHBG054359.
    InParanoidiP57760.
    KOiK08856.
    OMAiTEDQILW.
    OrthoDBiEOG7Z3F55.
    PhylomeDBiP57760.
    TreeFamiTF350433.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P57760-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGHALCVCSR GTVIIDNKRY LFVQKLGEGG FSYVDLVEGL HDGHFYALKR    50
    ILCHEQQDQE EAQREADMHR LFQHPNILRL MAYSLKERGA KHEAWLLLPF 100
    FKRGTLWNEI ERLKDQGNFL TEDQILPLLL GICRGLEAIH AKGYAHRDLK 150
    PTNILLGDEG QPVLMDLGSM NQACIQVEGS RQALALQDWA AQRCTISYRA 200
    PELFSVQSHC VIDERTDVWS LGCVLYAMMF GEGPYDMVFQ KGDSVALAVQ 250
    NDLSIPQSPR HSSALRQLLA SMMTVDPQQR PHIPVLLSQL EALQPPAPGQ 300
    HTTQI 305
    Length:305
    Mass (Da):34,409
    Last modified:January 23, 2007 - v2
    Checksum:i16E255E4732221CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86220 mRNA. Translation: BAB16310.1.
    RefSeqiNP_775165.1. NM_173142.1.
    UniGeneiRn.13308.

    Genome annotation databases

    EnsembliENSRNOT00000026194; ENSRNOP00000026194; ENSRNOG00000019294.
    GeneIDi286927.
    KEGGirno:286927.
    UCSCiRGD:629474. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86220 mRNA. Translation: BAB16310.1 .
    RefSeqi NP_775165.1. NM_173142.1.
    UniGenei Rn.13308.

    3D structure databases

    ProteinModelPortali P57760.
    SMRi P57760. Positions 13-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4570539.
    STRINGi 10116.ENSRNOP00000026194.

    PTM databases

    PhosphoSitei P57760.

    Proteomic databases

    PRIDEi P57760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026194 ; ENSRNOP00000026194 ; ENSRNOG00000019294 .
    GeneIDi 286927.
    KEGGi rno:286927.
    UCSCi RGD:629474. rat.

    Organism-specific databases

    CTDi 8576.
    RGDi 629474. Stk16.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000075037.
    HOGENOMi HOG000157694.
    HOVERGENi HBG054359.
    InParanoidi P57760.
    KOi K08856.
    OMAi TEDQILW.
    OrthoDBi EOG7Z3F55.
    PhylomeDBi P57760.
    TreeFami TF350433.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 5301.

    Miscellaneous databases

    NextBioi 625088.
    PROi P57760.

    Gene expression databases

    Genevestigatori P57760.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway."
      Ohta S., Takeuchi M., Deguchi M., Tsuji T., Gahara Y., Nagata K.
      Biochem. J. 350:395-404(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiSTK16_RAT
    AccessioniPrimary (citable) accession number: P57760
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3