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P57760 (STK16_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase 16

EC=2.7.11.1
Alternative name(s):
Myristoylated and palmitoylated serine/threonine-protein kinase
Short name=MPSK
Protein kinase PKL12
TGF-beta-stimulated factor 1
Short name=TSF-1
Tyrosine-protein kinase STK16
EC=2.7.10.2
Gene names
Name:Stk16
Synonyms:Mpsk1, Tsf1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates By similarity. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Monomer By similarity. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1 By similarity.

Subcellular location

Cytoplasmperinuclear region. Membrane; Lipid-anchor By similarity. Note: Associates with Golgi and Golgi-derived vesicles By similarity.

Tissue specificity

Expressed in heart, liver, brain, spleen, lung, skeletal muscle, kidney and testis. Ref.1

Induction

By TGF-beta. Ref.1

Post-translational modification

Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198 By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 305304Serine/threonine-protein kinase 16
PRO_0000086703

Regions

Domain20 – 293274Protein kinase
Nucleotide binding26 – 349ATP By similarity
Region166 – 20237Activation loop By similarity

Sites

Active site1481Proton acceptor By similarity
Binding site491ATP By similarity

Amino acid modifications

Modified residue1971Phosphoserine; by autocatalysis By similarity
Modified residue1981Phosphotyrosine; by autocatalysis By similarity
Lipidation21N-myristoyl glycine By similarity
Lipidation61S-palmitoyl cysteine By similarity
Lipidation81S-palmitoyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
P57760 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 16E255E4732221CB

FASTA30534,409
        10         20         30         40         50         60 
MGHALCVCSR GTVIIDNKRY LFVQKLGEGG FSYVDLVEGL HDGHFYALKR ILCHEQQDQE 

        70         80         90        100        110        120 
EAQREADMHR LFQHPNILRL MAYSLKERGA KHEAWLLLPF FKRGTLWNEI ERLKDQGNFL 

       130        140        150        160        170        180 
TEDQILPLLL GICRGLEAIH AKGYAHRDLK PTNILLGDEG QPVLMDLGSM NQACIQVEGS 

       190        200        210        220        230        240 
RQALALQDWA AQRCTISYRA PELFSVQSHC VIDERTDVWS LGCVLYAMMF GEGPYDMVFQ 

       250        260        270        280        290        300 
KGDSVALAVQ NDLSIPQSPR HSSALRQLLA SMMTVDPQQR PHIPVLLSQL EALQPPAPGQ 


HTTQI 

« Hide

References

[1]"A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway."
Ohta S., Takeuchi M., Deguchi M., Tsuji T., Gahara Y., Nagata K.
Biochem. J. 350:395-404(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, TISSUE SPECIFICITY, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86220 mRNA. Translation: BAB16310.1.
RefSeqNP_775165.1. NM_173142.1.
UniGeneRn.13308.

3D structure databases

ProteinModelPortalP57760.
SMRP57760. Positions 13-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4570539.
STRING10116.ENSRNOP00000026194.

PTM databases

PhosphoSiteP57760.

Proteomic databases

PRIDEP57760.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026194; ENSRNOP00000026194; ENSRNOG00000019294.
GeneID286927.
KEGGrno:286927.
UCSCRGD:629474. rat.

Organism-specific databases

CTD8576.
RGD629474. Stk16.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000075037.
HOGENOMHOG000157694.
HOVERGENHBG054359.
InParanoidP57760.
KOK08856.
OMACIIDERT.
OrthoDBEOG7Z3F55.
PhylomeDBP57760.
TreeFamTF350433.

Enzyme and pathway databases

BRENDA2.7.11.1. 5301.

Gene expression databases

GenevestigatorP57760.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio625088.
PROP57760.

Entry information

Entry nameSTK16_RAT
AccessionPrimary (citable) accession number: P57760
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families