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Protein

Serine/threonine-protein kinase 16

Gene

Stk16

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates (By similarity). May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei148 – 1481Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein kinase activity Source: RGD
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: RGD

GO - Biological processi

  1. cellular response to transforming growth factor beta stimulus Source: RGD
  2. positive regulation of transcription from RNA polymerase II promoter Source: RGD
  3. protein autophosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 16 (EC:2.7.11.1)
Alternative name(s):
Myristoylated and palmitoylated serine/threonine-protein kinase
Short name:
MPSK
Protein kinase PKL12
TGF-beta-stimulated factor 1
Short name:
TSF-1
Tyrosine-protein kinase STK16 (EC:2.7.10.2)
Gene namesi
Name:Stk16
Synonyms:Mpsk1, Tsf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi629474. Stk16.

Subcellular locationi

Cytoplasmperinuclear region. Membrane By similarity; Lipid-anchor By similarity
Note: Associates with Golgi and Golgi-derived vesicles.By similarity

GO - Cellular componenti

  1. Golgi-associated vesicle Source: Ensembl
  2. membrane Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 305304Serine/threonine-protein kinase 16PRO_0000086703Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi6 – 61S-palmitoyl cysteineBy similarity
Lipidationi8 – 81S-palmitoyl cysteineBy similarity
Modified residuei197 – 1971Phosphoserine; by autocatalysisBy similarity
Modified residuei198 – 1981Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198 (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP57760.

PTM databases

PhosphoSiteiP57760.

Expressioni

Tissue specificityi

Expressed in heart, liver, brain, spleen, lung, skeletal muscle, kidney and testis.1 Publication

Inductioni

By TGF-beta.1 Publication

Gene expression databases

GenevestigatoriP57760.

Interactioni

Subunit structurei

Monomer. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1.By similarity

Protein-protein interaction databases

MINTiMINT-4570539.
STRINGi10116.ENSRNOP00000026194.

Structurei

3D structure databases

ProteinModelPortaliP57760.
SMRiP57760. Positions 13-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 293274Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 20237Activation loopBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000075037.
HOGENOMiHOG000157694.
HOVERGENiHBG054359.
InParanoidiP57760.
KOiK08856.
OMAiTEDQILW.
OrthoDBiEOG7Z3F55.
PhylomeDBiP57760.
TreeFamiTF350433.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57760-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGHALCVCSR GTVIIDNKRY LFVQKLGEGG FSYVDLVEGL HDGHFYALKR
60 70 80 90 100
ILCHEQQDQE EAQREADMHR LFQHPNILRL MAYSLKERGA KHEAWLLLPF
110 120 130 140 150
FKRGTLWNEI ERLKDQGNFL TEDQILPLLL GICRGLEAIH AKGYAHRDLK
160 170 180 190 200
PTNILLGDEG QPVLMDLGSM NQACIQVEGS RQALALQDWA AQRCTISYRA
210 220 230 240 250
PELFSVQSHC VIDERTDVWS LGCVLYAMMF GEGPYDMVFQ KGDSVALAVQ
260 270 280 290 300
NDLSIPQSPR HSSALRQLLA SMMTVDPQQR PHIPVLLSQL EALQPPAPGQ

HTTQI
Length:305
Mass (Da):34,409
Last modified:January 23, 2007 - v2
Checksum:i16E255E4732221CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86220 mRNA. Translation: BAB16310.1.
RefSeqiNP_775165.1. NM_173142.1.
UniGeneiRn.13308.

Genome annotation databases

EnsembliENSRNOT00000026194; ENSRNOP00000026194; ENSRNOG00000019294.
GeneIDi286927.
KEGGirno:286927.
UCSCiRGD:629474. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86220 mRNA. Translation: BAB16310.1.
RefSeqiNP_775165.1. NM_173142.1.
UniGeneiRn.13308.

3D structure databases

ProteinModelPortaliP57760.
SMRiP57760. Positions 13-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4570539.
STRINGi10116.ENSRNOP00000026194.

PTM databases

PhosphoSiteiP57760.

Proteomic databases

PRIDEiP57760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026194; ENSRNOP00000026194; ENSRNOG00000019294.
GeneIDi286927.
KEGGirno:286927.
UCSCiRGD:629474. rat.

Organism-specific databases

CTDi8576.
RGDi629474. Stk16.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000075037.
HOGENOMiHOG000157694.
HOVERGENiHBG054359.
InParanoidiP57760.
KOiK08856.
OMAiTEDQILW.
OrthoDBiEOG7Z3F55.
PhylomeDBiP57760.
TreeFamiTF350433.

Enzyme and pathway databases

BRENDAi2.7.11.1. 5301.

Miscellaneous databases

NextBioi625088.
PROiP57760.

Gene expression databases

GenevestigatoriP57760.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel transcriptional factor with Ser/Thr kinase activity involved in the transforming growth factor (TGF)-beta signalling pathway."
    Ohta S., Takeuchi M., Deguchi M., Tsuji T., Gahara Y., Nagata K.
    Biochem. J. 350:395-404(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], POSSIBLE FUNCTION, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiSTK16_RAT
AccessioniPrimary (citable) accession number: P57760
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.