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Protein

UTP--glucose-1-phosphate uridylyltransferase 1

Gene

UGP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Converts glucose 1-phosphate to UDP-glucose, which is the major glycosyl donor for polysaccharides. Acts redundantly with UGP2 and is essential for the synthesis of sucrose, starch and cell wall, and callose deposition (PubMed:19366709, PubMed:20435647). Involved in the regulation of the programmed cell death (PCD) induced by the fungal toxin fumonisin B1 (FB1) (PubMed:23438466).3 Publications

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001UTPBy similarity
Binding sitei163 – 1631UTPBy similarity
Binding sitei192 – 1921UTP; via amide nitrogenBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei223 – 2231UTPBy similarity
Binding sitei361 – 3611UTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • callose deposition in cell wall Source: TAIR
  • pollen development Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to salt stress Source: TAIR
  • UDP-glucose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT5G17310-MONOMER.
ARA:GQT-1684-MONOMER.
BRENDAi2.7.7.9. 399.
ReactomeiR-ATH-173599. Formation of the active cofactor, UDP-glucuronate.
R-ATH-3322077. Glycogen synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase 1 (EC:2.7.7.9)
Alternative name(s):
UDP-glucose pyrophosphorylase 1
Short name:
AtUGP11 Publication
Short name:
UDPGP 1
Short name:
UGPase 1
Gene namesi
Name:UGP11 Publication
Ordered Locus Names:At5g17310
ORF Names:MKP11.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G17310.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduced number of seeds (PubMed:19366709). The double mutants upg1 and ugp2 display severe growth defects and male sterility due to the absence of callose deposition around microspores (PubMed:20435647).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 470469UTP--glucose-1-phosphate uridylyltransferase 1PRO_0000185756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP57751.
PRIDEiP57751.

PTM databases

iPTMnetiP57751.

Expressioni

Tissue specificityi

Expressed in roots, rosette leaves, cauline leaves, stems, flowers and siliques.1 Publication

Inductioni

By sucrose.1 Publication

Gene expression databases

ExpressionAtlasiP57751. baseline and differential.
GenevisibleiP57751. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G17310.2.

Structurei

3D structure databases

ProteinModelPortaliP57751.
SMRiP57751. Positions 9-470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 894UTP bindingBy similarity
Regioni88 – 892Substrate bindingBy similarity
Regioni221 – 2233Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

eggNOGiKOG2638. Eukaryota.
COG4284. LUCA.
HOGENOMiHOG000113618.
InParanoidiP57751.
KOiK00963.
OMAiRMVANEQ.
PhylomeDBiP57751.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR016267. UDPGP_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P57751-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATATEKLP QLKSAVDGLT EMSENEKSGF INLVSRYLSG EAQHIEWSKI
60 70 80 90 100
QTPTDEIVVP YDKMANVSED ASETKYLLDK LVVLKLNGGL GTTMGCTGPK
110 120 130 140 150
SVIEVRDGLT FLDLIVIQIE NLNNKYNCKV PLVLMNSFNT HDDTQKIVEK
160 170 180 190 200
YTKSNVDIHT FNQSKYPRVV ADEFVPWPSK GKTDKDGWYP PGHGDVFPSL
210 220 230 240 250
MNSGKLDAFL SQGKEYVFIA NSDNLGAIVD LKILKHLIQN KNEYCMEVTP
260 270 280 290 300
KTLADVKGGT LISYEGKVQL LEIAQVPDEH VNEFKSIEKF KIFNTNNLWV
310 320 330 340 350
NLKAIKKLVE ADALKMEIIP NPKEVDGVKV LQLETAAGAA IRFFDNAIGV
360 370 380 390 400
NVPRSRFLPV KATSDLLLVQ SDLYTLVDGF VTRNKARTNP TNPAIELGPE
410 420 430 440 450
FKKVASFLSR FKSIPSIVEL DSLKVSGDVW FGSGVVLKGK VTVKANAGTK
460 470
LEIPDNAVLE NKDINGPEDL
Length:470
Mass (Da):51,920
Last modified:January 24, 2001 - v1
Checksum:i488C4A034DC73AE8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005238 Genomic DNA. Translation: BAB10518.1.
CP002688 Genomic DNA. Translation: AED92412.1.
AF360244 mRNA. Translation: AAK25954.1.
AY040042 mRNA. Translation: AAK64100.1.
AF361816 mRNA. Translation: AAK32829.1.
RefSeqiNP_197233.1. NM_121737.3. [P57751-1]
UniGeneiAt.20807.

Genome annotation databases

EnsemblPlantsiAT5G17310.2; AT5G17310.2; AT5G17310. [P57751-1]
GeneIDi831596.
KEGGiath:AT5G17310.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005238 Genomic DNA. Translation: BAB10518.1.
CP002688 Genomic DNA. Translation: AED92412.1.
AF360244 mRNA. Translation: AAK25954.1.
AY040042 mRNA. Translation: AAK64100.1.
AF361816 mRNA. Translation: AAK32829.1.
RefSeqiNP_197233.1. NM_121737.3. [P57751-1]
UniGeneiAt.20807.

3D structure databases

ProteinModelPortaliP57751.
SMRiP57751. Positions 9-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G17310.2.

PTM databases

iPTMnetiP57751.

Proteomic databases

PaxDbiP57751.
PRIDEiP57751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G17310.2; AT5G17310.2; AT5G17310. [P57751-1]
GeneIDi831596.
KEGGiath:AT5G17310.

Organism-specific databases

TAIRiAT5G17310.

Phylogenomic databases

eggNOGiKOG2638. Eukaryota.
COG4284. LUCA.
HOGENOMiHOG000113618.
InParanoidiP57751.
KOiK00963.
OMAiRMVANEQ.
PhylomeDBiP57751.

Enzyme and pathway databases

BioCyciARA:AT5G17310-MONOMER.
ARA:GQT-1684-MONOMER.
BRENDAi2.7.7.9. 399.
ReactomeiR-ATH-173599. Formation of the active cofactor, UDP-glucuronate.
R-ATH-3322077. Glycogen synthesis.

Miscellaneous databases

PROiP57751.

Gene expression databases

ExpressionAtlasiP57751. baseline and differential.
GenevisibleiP57751. AT.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR016267. UDPGP_trans.
IPR002618. UDPGP_trans_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "UDP-glucose pyrophosphorylase is not rate limiting, but is essential in Arabidopsis."
    Meng M., Geisler M., Johansson H., Harholt J., Scheller H.V., Mellerowicz E.J., Kleczkowski L.A.
    Plant Cell Physiol. 50:998-1011(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION BY SUCROSE, DISRUPTION PHENOTYPE.
  5. "UDP-glucose pyrophosphorylase is rate limiting in vegetative and reproductive phases in Arabidopsis thaliana."
    Park J.I., Ishimizu T., Suwabe K., Sudo K., Masuko H., Hakozaki H., Nou I.S., Suzuki G., Watanabe M.
    Plant Cell Physiol. 51:981-996(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "UDP-glucose pyrophosphorylase is a novel plant cell death regulator."
    Chivasa S., Tome D.F., Slabas A.R.
    J. Proteome Res. 12:1743-1753(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiUGPA1_ARATH
AccessioniPrimary (citable) accession number: P57751
Secondary accession number(s): Q3E9G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: April 13, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.