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Protein

Matrix metalloproteinase-20

Gene

Mmp20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation (By similarity).By similarity

Catalytic activityi

Cleaves aggrecan at the 360-Asn-|-Phe-361 site.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
  • Ca2+By similarityNote: Binds 2 Calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Zinc 1; in inhibited formBy similarity1
Metal bindingi163Calcium 1By similarity1
Metal bindingi164Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi165Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi175Zinc 2By similarity1
Metal bindingi177Zinc 2By similarity1
Metal bindingi182Calcium 2By similarity1
Metal bindingi183Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi185Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi187Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi190Zinc 2By similarity1
Metal bindingi196Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi197Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi199Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi201Calcium 1By similarity1
Metal bindingi203Zinc 2By similarity1
Metal bindingi205Calcium 2By similarity1
Metal bindingi208Calcium 2By similarity1
Metal bindingi225Zinc 1; catalyticBy similarity1
Active sitei226PROSITE-ProRule annotation1
Metal bindingi229Zinc 1; catalyticBy similarity1
Metal bindingi235Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • amelogenesis Source: MGI
  • extracellular matrix disassembly Source: MGI
  • protein catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.24.B6. 3474.
ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-1474228. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiM10.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-20 (EC:3.4.24.-)
Short name:
MMP-20
Alternative name(s):
Enamel metalloproteinase
Enamelysin
Gene namesi
Name:Mmp20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1353466. Mmp20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002883522 – 106By similarityAdd BLAST85
ChainiPRO_0000028836107 – 482Matrix metalloproteinase-20Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi295 ↔ 482By similarity

Post-translational modificationi

Autoactivates at least at the 106-Asn-|-Tyr-107 site.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Zymogen

Proteomic databases

PaxDbiP57748.
PRIDEiP57748.

PTM databases

PhosphoSitePlusiP57748.

Expressioni

Tissue specificityi

Expressed specifically in the enamel organ.

Gene expression databases

BgeeiENSMUSG00000018620.
CleanExiMM_MMP20.
GenevisibleiP57748. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034487.

Structurei

3D structure databases

ProteinModelPortaliP57748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati292 – 342Hemopexin 1Add BLAST51
Repeati343 – 388Hemopexin 2Add BLAST46
Repeati390 – 438Hemopexin 3Add BLAST49
Repeati439 – 482Hemopexin 4Add BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 104Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiP57748.
KOiK07999.
OMAiYSYDERK.
OrthoDBiEOG091G03DP.
PhylomeDBiP57748.
TreeFamiTF315428.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLPASGLA VLVTALKFAT ADPNLLAATP RTFRSNYHLA QAYLDKYYTK
60 70 80 90 100
KGGPQAGEMV ARESNPMIRR IKELQIFFGL KVTGKLDQNT MNVIKKPRCG
110 120 130 140 150
VPDVANYRLF PGEPKWKKNI LTYRISKYTP SMSPTEVDKA IQMALHAWST
160 170 180 190 200
AVPLNFVRIN SGEADIMISF ETGDHGDSYP FDGPRGTLAH AFAPGEGLGG
210 220 230 240 250
DTHFDNAEKW TMGTNGFNLF TVAAHEFGHA LGLGHSTDPS ALMYPTYKYQ
260 270 280 290 300
NPYRFHLPKD DVKGIQALYG PRKIFPGKPT MPHIPPHKPS IPDLCDSSSS
310 320 330 340 350
FDAVTMLGKE LLFFKDRIFW RRQVHLPTGI RPSTITSSFP QLMSNVDAAY
360 370 380 390 400
EVAERGIAFF FKGPHYWVTR GFHMQGPPRT IYDFGFPRHV QRIDAAVYLK
410 420 430 440 450
EPQKTLFFVG EEYYSYDERK KKMEKDYPKN TEEEFSGVSG HIDAAVELNG
460 470 480
YIYFFSGRKT FKYDTEKEDV VSVVKSSSWI GC
Length:482
Mass (Da):54,373
Last modified:January 24, 2001 - v1
Checksum:i366149FAF2BDC8BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156956
, AF156947, AF156948, AF156949, AF156950, AF156951, AF156952, AF156953, AF156954, AF156955 Genomic DNA. Translation: AAF28472.1.
AF155933 mRNA. Translation: AAF28470.1.
BC152335 mRNA. Translation: AAI52336.1.
BC152336 mRNA. Translation: AAI52337.1.
CCDSiCCDS22809.1.
RefSeqiNP_038931.1. NM_013903.2.
UniGeneiMm.390121.

Genome annotation databases

EnsembliENSMUST00000034487; ENSMUSP00000034487; ENSMUSG00000018620.
GeneIDi30800.
KEGGimmu:30800.
UCSCiuc009ocu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156956
, AF156947, AF156948, AF156949, AF156950, AF156951, AF156952, AF156953, AF156954, AF156955 Genomic DNA. Translation: AAF28472.1.
AF155933 mRNA. Translation: AAF28470.1.
BC152335 mRNA. Translation: AAI52336.1.
BC152336 mRNA. Translation: AAI52337.1.
CCDSiCCDS22809.1.
RefSeqiNP_038931.1. NM_013903.2.
UniGeneiMm.390121.

3D structure databases

ProteinModelPortaliP57748.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034487.

Protein family/group databases

MEROPSiM10.019.

PTM databases

PhosphoSitePlusiP57748.

Proteomic databases

PaxDbiP57748.
PRIDEiP57748.

Protocols and materials databases

DNASUi30800.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034487; ENSMUSP00000034487; ENSMUSG00000018620.
GeneIDi30800.
KEGGimmu:30800.
UCSCiuc009ocu.1. mouse.

Organism-specific databases

CTDi9313.
MGIiMGI:1353466. Mmp20.

Phylogenomic databases

eggNOGiKOG1565. Eukaryota.
ENOG410XQ5D. LUCA.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiP57748.
KOiK07999.
OMAiYSYDERK.
OrthoDBiEOG091G03DP.
PhylomeDBiP57748.
TreeFamiTF315428.

Enzyme and pathway databases

BRENDAi3.4.24.B6. 3474.
ReactomeiR-MMU-1442490. Collagen degradation.
R-MMU-1474228. Degradation of the extracellular matrix.

Miscellaneous databases

PROiP57748.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018620.
CleanExiMM_MMP20.
GenevisibleiP57748. MM.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP20_MOUSE
AccessioniPrimary (citable) accession number: P57748
Secondary accession number(s): A7MCV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.