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P57748

- MMP20_MOUSE

UniProt

P57748 - MMP20_MOUSE

Protein

Matrix metalloproteinase-20

Gene

Mmp20

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (24 Jan 2001)
      Previous versions | rss
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    Functioni

    Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation By similarity.By similarity

    Catalytic activityi

    Cleaves aggrecan at the 360-Asn-|-Phe-361 site.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity
    Binds 2 Calcium ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991Zinc 1; in inhibited formBy similarity
    Metal bindingi163 – 1631Calcium 1By similarity
    Metal bindingi164 – 1641Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi165 – 1651Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi175 – 1751Zinc 2By similarity
    Metal bindingi177 – 1771Zinc 2By similarity
    Metal bindingi182 – 1821Calcium 2By similarity
    Metal bindingi183 – 1831Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi185 – 1851Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi187 – 1871Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi190 – 1901Zinc 2By similarity
    Metal bindingi196 – 1961Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi197 – 1971Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi199 – 1991Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi201 – 2011Calcium 1By similarity
    Metal bindingi203 – 2031Zinc 2By similarity
    Metal bindingi205 – 2051Calcium 2By similarity
    Metal bindingi208 – 2081Calcium 2By similarity
    Metal bindingi225 – 2251Zinc 1; catalyticBy similarity
    Active sitei226 – 2261PROSITE-ProRule annotation
    Metal bindingi229 – 2291Zinc 1; catalyticBy similarity
    Metal bindingi235 – 2351Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. metalloendopeptidase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. amelogenesis Source: MGI
    2. extracellular matrix disassembly Source: MGI
    3. protein catabolic process Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199052. Degradation of the extracellular matrix.
    REACT_199055. Collagen degradation.

    Protein family/group databases

    MEROPSiM10.019.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Matrix metalloproteinase-20 (EC:3.4.24.-)
    Short name:
    MMP-20
    Alternative name(s):
    Enamel metalloproteinase
    Enamelysin
    Gene namesi
    Name:Mmp20
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1353466. Mmp20.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: Reactome
    2. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Propeptidei22 – 10685By similarityPRO_0000028835Add
    BLAST
    Chaini107 – 482376Matrix metalloproteinase-20PRO_0000028836Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi295 ↔ 482By similarity

    Post-translational modificationi

    Autoactivates at least at the 106-Asn-|-Tyr-107 site.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Disulfide bond, Zymogen

    Proteomic databases

    PRIDEiP57748.

    PTM databases

    PhosphoSiteiP57748.

    Expressioni

    Tissue specificityi

    Expressed specifically in the enamel organ.

    Gene expression databases

    BgeeiP57748.
    CleanExiMM_MMP20.
    GenevestigatoriP57748.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034487.

    Structurei

    3D structure databases

    ProteinModelPortaliP57748.
    SMRiP57748. Positions 39-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati292 – 34251Hemopexin 1Add
    BLAST
    Repeati343 – 38846Hemopexin 2Add
    BLAST
    Repeati390 – 43849Hemopexin 3Add
    BLAST
    Repeati439 – 48244Hemopexin 4Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi97 – 1048Cysteine switchBy similarity

    Domaini

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    Sequence similaritiesi

    Belongs to the peptidase M10A family.Curated
    Contains 4 hemopexin repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG328389.
    GeneTreeiENSGT00750000117336.
    HOGENOMiHOG000217927.
    HOVERGENiHBG052484.
    InParanoidiA7MCV5.
    KOiK07999.
    OMAiPDVANYR.
    OrthoDBiEOG7XPZ57.
    PhylomeDBiP57748.
    TreeFamiTF315428.

    Family and domain databases

    Gene3Di2.110.10.10. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR024079. MetalloPept_cat_dom.
    IPR028716. MMP20.
    IPR001818. Pept_M10_metallopeptidase.
    IPR021190. Pept_M10A.
    IPR016293. Pept_M10A_stromelysin-type.
    IPR021158. Pept_M10A_Zn_BS.
    IPR006026. Peptidase_Metallo.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view]
    PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
    PfamiPF00045. Hemopexin. 4 hits.
    PF00413. Peptidase_M10. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
    PRINTSiPR00138. MATRIXIN.
    SMARTiSM00120. HX. 4 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47090. SSF47090. 1 hit.
    SSF50923. SSF50923. 1 hit.
    PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
    PS51642. HEMOPEXIN_2. 4 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P57748-1 [UniParc]FASTAAdd to Basket

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    MKVLPASGLA VLVTALKFAT ADPNLLAATP RTFRSNYHLA QAYLDKYYTK    50
    KGGPQAGEMV ARESNPMIRR IKELQIFFGL KVTGKLDQNT MNVIKKPRCG 100
    VPDVANYRLF PGEPKWKKNI LTYRISKYTP SMSPTEVDKA IQMALHAWST 150
    AVPLNFVRIN SGEADIMISF ETGDHGDSYP FDGPRGTLAH AFAPGEGLGG 200
    DTHFDNAEKW TMGTNGFNLF TVAAHEFGHA LGLGHSTDPS ALMYPTYKYQ 250
    NPYRFHLPKD DVKGIQALYG PRKIFPGKPT MPHIPPHKPS IPDLCDSSSS 300
    FDAVTMLGKE LLFFKDRIFW RRQVHLPTGI RPSTITSSFP QLMSNVDAAY 350
    EVAERGIAFF FKGPHYWVTR GFHMQGPPRT IYDFGFPRHV QRIDAAVYLK 400
    EPQKTLFFVG EEYYSYDERK KKMEKDYPKN TEEEFSGVSG HIDAAVELNG 450
    YIYFFSGRKT FKYDTEKEDV VSVVKSSSWI GC 482
    Length:482
    Mass (Da):54,373
    Last modified:January 24, 2001 - v1
    Checksum:i366149FAF2BDC8BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156956
    , AF156947, AF156948, AF156949, AF156950, AF156951, AF156952, AF156953, AF156954, AF156955 Genomic DNA. Translation: AAF28472.1.
    AF155933 mRNA. Translation: AAF28470.1.
    BC152335 mRNA. Translation: AAI52336.1.
    BC152336 mRNA. Translation: AAI52337.1.
    CCDSiCCDS22809.1.
    RefSeqiNP_038931.1. NM_013903.2.
    UniGeneiMm.390121.

    Genome annotation databases

    EnsembliENSMUST00000034487; ENSMUSP00000034487; ENSMUSG00000018620.
    GeneIDi30800.
    KEGGimmu:30800.
    UCSCiuc009ocu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF156956
    , AF156947 , AF156948 , AF156949 , AF156950 , AF156951 , AF156952 , AF156953 , AF156954 , AF156955 Genomic DNA. Translation: AAF28472.1 .
    AF155933 mRNA. Translation: AAF28470.1 .
    BC152335 mRNA. Translation: AAI52336.1 .
    BC152336 mRNA. Translation: AAI52337.1 .
    CCDSi CCDS22809.1.
    RefSeqi NP_038931.1. NM_013903.2.
    UniGenei Mm.390121.

    3D structure databases

    ProteinModelPortali P57748.
    SMRi P57748. Positions 39-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000034487.

    Protein family/group databases

    MEROPSi M10.019.

    PTM databases

    PhosphoSitei P57748.

    Proteomic databases

    PRIDEi P57748.

    Protocols and materials databases

    DNASUi 30800.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034487 ; ENSMUSP00000034487 ; ENSMUSG00000018620 .
    GeneIDi 30800.
    KEGGi mmu:30800.
    UCSCi uc009ocu.1. mouse.

    Organism-specific databases

    CTDi 9313.
    MGIi MGI:1353466. Mmp20.

    Phylogenomic databases

    eggNOGi NOG328389.
    GeneTreei ENSGT00750000117336.
    HOGENOMi HOG000217927.
    HOVERGENi HBG052484.
    InParanoidi A7MCV5.
    KOi K07999.
    OMAi PDVANYR.
    OrthoDBi EOG7XPZ57.
    PhylomeDBi P57748.
    TreeFami TF315428.

    Enzyme and pathway databases

    Reactomei REACT_199052. Degradation of the extracellular matrix.
    REACT_199055. Collagen degradation.

    Miscellaneous databases

    NextBioi 307218.
    PROi P57748.
    SOURCEi Search...

    Gene expression databases

    Bgeei P57748.
    CleanExi MM_MMP20.
    Genevestigatori P57748.

    Family and domain databases

    Gene3Di 2.110.10.10. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR024079. MetalloPept_cat_dom.
    IPR028716. MMP20.
    IPR001818. Pept_M10_metallopeptidase.
    IPR021190. Pept_M10A.
    IPR016293. Pept_M10A_stromelysin-type.
    IPR021158. Pept_M10A_Zn_BS.
    IPR006026. Peptidase_Metallo.
    IPR002477. Peptidoglycan-bd-like.
    [Graphical view ]
    PANTHERi PTHR10201:SF125. PTHR10201:SF125. 1 hit.
    Pfami PF00045. Hemopexin. 4 hits.
    PF00413. Peptidase_M10. 1 hit.
    PF01471. PG_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
    PRINTSi PR00138. MATRIXIN.
    SMARTi SM00120. HX. 4 hits.
    SM00235. ZnMc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47090. SSF47090. 1 hit.
    SSF50923. SSF50923. 1 hit.
    PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
    PS51642. HEMOPEXIN_2. 4 hits.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, characterization, and chromosomal location of the mouse enamelysin gene."
      Caterina J., Shi J., Krakora S., Bartlett J.D., Engler J.A., Kozak C.A., Birkedal-Hansen H.
      Genomics 62:308-311(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/SvJ.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiMMP20_MOUSE
    AccessioniPrimary (citable) accession number: P57748
    Secondary accession number(s): A7MCV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: January 24, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3