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P57748

- MMP20_MOUSE

UniProt

P57748 - MMP20_MOUSE

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Protein

Matrix metalloproteinase-20

Gene

Mmp20

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation (By similarity).By similarity

Catalytic activityi

Cleaves aggrecan at the 360-Asn-|-Phe-361 site.

Cofactori

Binds 2 zinc ions per subunit.By similarity
Binds 2 Calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Zinc 1; in inhibited formBy similarity
Metal bindingi163 – 1631Calcium 1By similarity
Metal bindingi164 – 1641Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi165 – 1651Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi175 – 1751Zinc 2By similarity
Metal bindingi177 – 1771Zinc 2By similarity
Metal bindingi182 – 1821Calcium 2By similarity
Metal bindingi183 – 1831Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi185 – 1851Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi187 – 1871Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi190 – 1901Zinc 2By similarity
Metal bindingi196 – 1961Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi197 – 1971Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi199 – 1991Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi201 – 2011Calcium 1By similarity
Metal bindingi203 – 2031Zinc 2By similarity
Metal bindingi205 – 2051Calcium 2By similarity
Metal bindingi208 – 2081Calcium 2By similarity
Metal bindingi225 – 2251Zinc 1; catalyticBy similarity
Active sitei226 – 2261PROSITE-ProRule annotation
Metal bindingi229 – 2291Zinc 1; catalyticBy similarity
Metal bindingi235 – 2351Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. metalloendopeptidase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. amelogenesis Source: MGI
  2. extracellular matrix disassembly Source: MGI
  3. protein catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_199052. Degradation of the extracellular matrix.
REACT_199055. Collagen degradation.

Protein family/group databases

MEROPSiM10.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-20 (EC:3.4.24.-)
Short name:
MMP-20
Alternative name(s):
Enamel metalloproteinase
Enamelysin
Gene namesi
Name:Mmp20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1353466. Mmp20.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Propeptidei22 – 10685By similarityPRO_0000028835Add
BLAST
Chaini107 – 482376Matrix metalloproteinase-20PRO_0000028836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi295 ↔ 482By similarity

Post-translational modificationi

Autoactivates at least at the 106-Asn-|-Tyr-107 site.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Zymogen

Proteomic databases

PRIDEiP57748.

PTM databases

PhosphoSiteiP57748.

Expressioni

Tissue specificityi

Expressed specifically in the enamel organ.

Gene expression databases

BgeeiP57748.
CleanExiMM_MMP20.
ExpressionAtlasiP57748. differential.
GenevestigatoriP57748.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034487.

Structurei

3D structure databases

ProteinModelPortaliP57748.
SMRiP57748. Positions 39-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati292 – 34251Hemopexin 1Add
BLAST
Repeati343 – 38846Hemopexin 2Add
BLAST
Repeati390 – 43849Hemopexin 3Add
BLAST
Repeati439 – 48244Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi97 – 1048Cysteine switchBy similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG328389.
GeneTreeiENSGT00760000118870.
HOGENOMiHOG000217927.
HOVERGENiHBG052484.
InParanoidiP57748.
KOiK07999.
OMAiPDVANYR.
OrthoDBiEOG7XPZ57.
PhylomeDBiP57748.
TreeFamiTF315428.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERiPTHR10201:SF125. PTHR10201:SF125. 1 hit.
PfamiPF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57748-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLPASGLA VLVTALKFAT ADPNLLAATP RTFRSNYHLA QAYLDKYYTK
60 70 80 90 100
KGGPQAGEMV ARESNPMIRR IKELQIFFGL KVTGKLDQNT MNVIKKPRCG
110 120 130 140 150
VPDVANYRLF PGEPKWKKNI LTYRISKYTP SMSPTEVDKA IQMALHAWST
160 170 180 190 200
AVPLNFVRIN SGEADIMISF ETGDHGDSYP FDGPRGTLAH AFAPGEGLGG
210 220 230 240 250
DTHFDNAEKW TMGTNGFNLF TVAAHEFGHA LGLGHSTDPS ALMYPTYKYQ
260 270 280 290 300
NPYRFHLPKD DVKGIQALYG PRKIFPGKPT MPHIPPHKPS IPDLCDSSSS
310 320 330 340 350
FDAVTMLGKE LLFFKDRIFW RRQVHLPTGI RPSTITSSFP QLMSNVDAAY
360 370 380 390 400
EVAERGIAFF FKGPHYWVTR GFHMQGPPRT IYDFGFPRHV QRIDAAVYLK
410 420 430 440 450
EPQKTLFFVG EEYYSYDERK KKMEKDYPKN TEEEFSGVSG HIDAAVELNG
460 470 480
YIYFFSGRKT FKYDTEKEDV VSVVKSSSWI GC
Length:482
Mass (Da):54,373
Last modified:January 24, 2001 - v1
Checksum:i366149FAF2BDC8BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF156956
, AF156947, AF156948, AF156949, AF156950, AF156951, AF156952, AF156953, AF156954, AF156955 Genomic DNA. Translation: AAF28472.1.
AF155933 mRNA. Translation: AAF28470.1.
BC152335 mRNA. Translation: AAI52336.1.
BC152336 mRNA. Translation: AAI52337.1.
CCDSiCCDS22809.1.
RefSeqiNP_038931.1. NM_013903.2.
UniGeneiMm.390121.

Genome annotation databases

EnsembliENSMUST00000034487; ENSMUSP00000034487; ENSMUSG00000018620.
GeneIDi30800.
KEGGimmu:30800.
UCSCiuc009ocu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF156956
, AF156947 , AF156948 , AF156949 , AF156950 , AF156951 , AF156952 , AF156953 , AF156954 , AF156955 Genomic DNA. Translation: AAF28472.1 .
AF155933 mRNA. Translation: AAF28470.1 .
BC152335 mRNA. Translation: AAI52336.1 .
BC152336 mRNA. Translation: AAI52337.1 .
CCDSi CCDS22809.1.
RefSeqi NP_038931.1. NM_013903.2.
UniGenei Mm.390121.

3D structure databases

ProteinModelPortali P57748.
SMRi P57748. Positions 39-482.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000034487.

Protein family/group databases

MEROPSi M10.019.

PTM databases

PhosphoSitei P57748.

Proteomic databases

PRIDEi P57748.

Protocols and materials databases

DNASUi 30800.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034487 ; ENSMUSP00000034487 ; ENSMUSG00000018620 .
GeneIDi 30800.
KEGGi mmu:30800.
UCSCi uc009ocu.1. mouse.

Organism-specific databases

CTDi 9313.
MGIi MGI:1353466. Mmp20.

Phylogenomic databases

eggNOGi NOG328389.
GeneTreei ENSGT00760000118870.
HOGENOMi HOG000217927.
HOVERGENi HBG052484.
InParanoidi P57748.
KOi K07999.
OMAi PDVANYR.
OrthoDBi EOG7XPZ57.
PhylomeDBi P57748.
TreeFami TF315428.

Enzyme and pathway databases

Reactomei REACT_199052. Degradation of the extracellular matrix.
REACT_199055. Collagen degradation.

Miscellaneous databases

NextBioi 307218.
PROi P57748.
SOURCEi Search...

Gene expression databases

Bgeei P57748.
CleanExi MM_MMP20.
ExpressionAtlasi P57748. differential.
Genevestigatori P57748.

Family and domain databases

Gene3Di 2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProi IPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR024079. MetalloPept_cat_dom.
IPR028716. MMP20.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view ]
PANTHERi PTHR10201:SF125. PTHR10201:SF125. 1 hit.
Pfami PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSi PR00138. MATRIXIN.
SMARTi SM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view ]
SUPFAMi SSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEi PS00546. CYSTEINE_SWITCH. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization, and chromosomal location of the mouse enamelysin gene."
    Caterina J., Shi J., Krakora S., Bartlett J.D., Engler J.A., Kozak C.A., Birkedal-Hansen H.
    Genomics 62:308-311(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiMMP20_MOUSE
AccessioniPrimary (citable) accession number: P57748
Secondary accession number(s): A7MCV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3