Matrix metalloproteinase-20 precursor

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P57748 (MMP20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Matrix metalloproteinase-20
Short name=MMP-20
EC=3.4.24.-

Alternative name(s):
Enamel metalloproteinase
Enamelysin

Gene names

Name:

Mmp20

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation By similarity.
Catalytic activity	Cleaves aggrecan at the 360-Asn-\|-Phe-361 site.
Cofactor	Binds 2 zinc ions per subunit By similarity. Binds 2 Calcium ions per subunit By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed specifically in the enamel organ.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	Autoactivates at least at the 106-Asn-\|-Tyr-107 site By similarity.
Sequence similarities	Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Repeat Signal
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Autocatalytic cleavage Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amelogenesis Inferred from mutant phenotype PubMed 15557396 PubMed 21454549. Source: MGI proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

Potential

Propeptide

22 – 106

By similarity

PRO_0000028835

Chain

107 – 482

376

Matrix metalloproteinase-20

PRO_0000028836

Regions

Domain

301 – 344

Hemopexin-like 1

Domain

346 – 388

Hemopexin-like 2

Domain

393 – 440

Hemopexin-like 3

Domain

442 – 482

Hemopexin-like 4

Motif

97 – 104

Cysteine switch By similarity

Sites

Active site

226

By similarity

Metal binding

Zinc 1; in inhibited form By similarity

Metal binding

163

Calcium 1 By similarity

Metal binding

164

Calcium 1; via carbonyl oxygen By similarity

Metal binding

165

Calcium 1; via carbonyl oxygen By similarity

Metal binding

175

Zinc 2 By similarity

Metal binding

177

Zinc 2 By similarity

Metal binding

182

Calcium 2 By similarity

Metal binding

183

Calcium 2; via carbonyl oxygen By similarity

Metal binding

185

Calcium 2; via carbonyl oxygen By similarity

Metal binding

187

Calcium 2; via carbonyl oxygen By similarity

Metal binding

190

Zinc 2 By similarity

Metal binding

196

Calcium 1; via carbonyl oxygen By similarity

Metal binding

197

Calcium 1; via carbonyl oxygen By similarity

Metal binding

199

Calcium 1; via carbonyl oxygen By similarity

Metal binding

201

Calcium 1 By similarity

Metal binding

203

Zinc 2 By similarity

Metal binding

205

Calcium 2 By similarity

Metal binding

208

Calcium 2 By similarity

Metal binding

225

Zinc 1; catalytic By similarity

Metal binding

229

Zinc 1; catalytic By similarity

Metal binding

235

Zinc 1; catalytic By similarity

Amino acid modifications

Disulfide bond

295 ↔ 482

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P57748 [UniParc].

Last modified January 24, 2001. Version 1.
Checksum: 366149FAF2BDC8BB
Show »

FASTA

482

54,373

        10         20         30         40         50         60 
MKVLPASGLA VLVTALKFAT ADPNLLAATP RTFRSNYHLA QAYLDKYYTK KGGPQAGEMV 

        70         80         90        100        110        120 
ARESNPMIRR IKELQIFFGL KVTGKLDQNT MNVIKKPRCG VPDVANYRLF PGEPKWKKNI 

       130        140        150        160        170        180 
LTYRISKYTP SMSPTEVDKA IQMALHAWST AVPLNFVRIN SGEADIMISF ETGDHGDSYP 

       190        200        210        220        230        240 
FDGPRGTLAH AFAPGEGLGG DTHFDNAEKW TMGTNGFNLF TVAAHEFGHA LGLGHSTDPS 

       250        260        270        280        290        300 
ALMYPTYKYQ NPYRFHLPKD DVKGIQALYG PRKIFPGKPT MPHIPPHKPS IPDLCDSSSS 

       310        320        330        340        350        360 
FDAVTMLGKE LLFFKDRIFW RRQVHLPTGI RPSTITSSFP QLMSNVDAAY EVAERGIAFF 

       370        380        390        400        410        420 
FKGPHYWVTR GFHMQGPPRT IYDFGFPRHV QRIDAAVYLK EPQKTLFFVG EEYYSYDERK 

       430        440        450        460        470        480 
KKMEKDYPKN TEEEFSGVSG HIDAAVELNG YIYFFSGRKT FKYDTEKEDV VSVVKSSSWI 


GC

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization, and chromosomal location of the mouse enamelysin gene." Caterina J., Shi J., Krakora S., Bartlett J.D., Engler J.A., Kozak C.A., Birkedal-Hansen H. Genomics 62:308-311(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/SvJ.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF156956 AF156955 Genomic DNA. Translation: AAF28472.1. AF155933 mRNA. Translation: AAF28470.1. BC152335 mRNA. Translation: AAI52336.1. BC152336 mRNA. Translation: AAI52337.1.
IPI	IPI00118789.
RefSeq	NP_038931.1. NM_013903.2.
UniGene	Mm.390121.
3D structure databases
ProteinModelPortal	P57748.
SMR	P57748. Positions 39-479.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000034487.
Protein family/group databases
MEROPS	M10.019.
PTM databases
PhosphoSite	P57748.
Proteomic databases
PRIDE	P57748.
Protocols and materials databases
DNASU	30800.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034487; ENSMUSP00000034487; ENSMUSG00000018620.
GeneID	30800.
KEGG	mmu:30800.
UCSC	uc009ocu.1. mouse.
Organism-specific databases
CTD	9313.
MGI	MGI:1353466. Mmp20.
Phylogenomic databases
eggNOG	NOG328389.
GeneTree	ENSGT00700000104046.
HOGENOM	HOG000217927.
HOVERGEN	HBG052484.
InParanoid	A7MCV5.
KO	K07999.
OMA	PDVANYR.
OrthoDB	EOG447FT2.
Gene expression databases
ArrayExpress	P57748.
Bgee	P57748.
CleanEx	MM_MMP20.
Genevestigator	P57748.
GermOnline	ENSMUSG00000018620. Mus musculus.
Family and domain databases
Gene3D	2.110.10.10. 1 hit. 3.40.390.10. 1 hit.
InterPro	IPR000585. Hemopexin-like_dom. IPR018487. Hemopexin-like_repeat. IPR024079. MetalloPept_cat_dom. IPR001818. Pept_M10_metallopeptidase. IPR021190. Pept_M10A. IPR016293. Pept_M10A_Metazoans. IPR021158. Pept_M10A_Zn_BS. IPR006026. Peptidase_Metallo. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Pfam	PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTS	PR00138. MATRIXIN.
SMART	SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view]
SUPFAM	SSF50923. Hemopexin. 1 hit. SSF47090. PGBD_like. 1 hit.
PROSITE	PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307218.
SOURCE	Search...

Entry information

Entry name

MMP20_MOUSE

Accession

Primary (citable) accession number: P57748
Secondary accession number(s): A7MCV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 24, 2001
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents