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P57744

- TIM8_YEAST

UniProt

P57744 - TIM8_YEAST

Protein

Mitochondrial import inner membrane translocase subunit TIM8

Gene

TIM8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex is non essential and only mediates the import of few proteins under precise conditions, while the predominant TIM9-TIM10 70 kDa complex is crucial and mediates the import of much more proteins. Strictly required for import of TIM23 in some conditions, when a low membrane potential exists in the mitochondria.5 Publications

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein transporter activity Source: SGD

    GO - Biological processi

    1. protein import into mitochondrial inner membrane Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31800-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM8
    Gene namesi
    Name:TIM8
    Ordered Locus Names:YJR135W-A
    ORF Names:YJR135BW
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR135w-a.
    SGDiS000007348. TIM8.

    Subcellular locationi

    Mitochondrion inner membrane 2 Publications; Peripheral membrane protein 2 Publications; Intermembrane side 2 Publications
    Note: Import into inner membrane protein requires TIM5 function.

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. mitochondrial intermembrane space Source: Reactome
    3. mitochondrial intermembrane space protein transporter complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8787Mitochondrial import inner membrane translocase subunit TIM8PRO_0000193594Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 68By similarity
    Disulfide bondi48 ↔ 64By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiP57744.
    PaxDbiP57744.
    PeptideAtlasiP57744.

    Expressioni

    Gene expression databases

    GenevestigatoriP57744.

    Interactioni

    Subunit structurei

    Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TIM13P532994EBI-9013892,EBI-9121

    Protein-protein interaction databases

    BioGridi33891. 20 interactions.
    DIPiDIP-8051N.
    IntActiP57744. 1 interaction.
    STRINGi4932.YJR135W-A.

    Structurei

    Secondary structure

    1
    87
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 4819
    Beta strandi53 – 564
    Helixi59 – 8527

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3CJHX-ray2.60B/D/F/H/J/L24-87[»]
    ProteinModelPortaliP57744.
    SMRiP57744. Positions 28-86.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP57744.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi44 – 6825Twin CX3C motifAdd
    BLAST

    Domaini

    The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM8 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane Probable.Curated

    Sequence similaritiesi

    Belongs to the small Tim family.Curated

    Phylogenomic databases

    eggNOGiNOG238740.
    GeneTreeiENSGT00390000016102.
    HOGENOMiHOG000115758.
    KOiK17780.
    OMAiEINEPIR.
    OrthoDBiEOG7DVDQJ.

    Family and domain databases

    Gene3Di1.10.287.810. 1 hit.
    InterProiIPR004217. Tim10/DDP_fam_Znf.
    [Graphical view]
    PfamiPF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view]
    SUPFAMiSSF144122. SSF144122. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P57744-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLSTSDLA SLDDTSKKEI ATFLEGENSK QKVQMSIHQF TNICFKKCVE   50
    SVNDSNLSSQ EEQCLSNCVN RFLDTNIRIV NGLQNTR 87
    Length:87
    Mass (Da):9,764
    Last modified:January 11, 2001 - v1
    Checksum:iB91D1DD41707EA24
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49636 Genomic DNA. No translation available.
    AY558258 Genomic DNA. Translation: AAS56584.1.
    BK006943 Genomic DNA. Translation: DAA08920.1.
    PIRiS78075.
    RefSeqiNP_058168.1. NM_001184382.1.

    Genome annotation databases

    EnsemblFungiiYJR135W-A; YJR135W-A; YJR135W-A.
    GeneIDi853600.
    KEGGisce:YJR135W-A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49636 Genomic DNA. No translation available.
    AY558258 Genomic DNA. Translation: AAS56584.1 .
    BK006943 Genomic DNA. Translation: DAA08920.1 .
    PIRi S78075.
    RefSeqi NP_058168.1. NM_001184382.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3CJH X-ray 2.60 B/D/F/H/J/L 24-87 [» ]
    ProteinModelPortali P57744.
    SMRi P57744. Positions 28-86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33891. 20 interactions.
    DIPi DIP-8051N.
    IntActi P57744. 1 interaction.
    STRINGi 4932.YJR135W-A.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P57744.
    PaxDbi P57744.
    PeptideAtlasi P57744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR135W-A ; YJR135W-A ; YJR135W-A .
    GeneIDi 853600.
    KEGGi sce:YJR135W-A.

    Organism-specific databases

    CYGDi YJR135w-a.
    SGDi S000007348. TIM8.

    Phylogenomic databases

    eggNOGi NOG238740.
    GeneTreei ENSGT00390000016102.
    HOGENOMi HOG000115758.
    KOi K17780.
    OMAi EINEPIR.
    OrthoDBi EOG7DVDQJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31800-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    EvolutionaryTracei P57744.
    NextBioi 974424.

    Gene expression databases

    Genevestigatori P57744.

    Family and domain databases

    Gene3Di 1.10.287.810. 1 hit.
    InterProi IPR004217. Tim10/DDP_fam_Znf.
    [Graphical view ]
    Pfami PF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF144122. SSF144122. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Different import pathways through the mitochondrial intermembrane space for inner membrane proteins."
      Leuenberger D., Bally N.A., Schatz G., Koehler C.M.
      EMBO J. 18:4816-4822(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH THE TIM22 COMPLEX.
    5. "Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway."
      Kurz M., Martin H., Rassow J., Pfanner N., Ryan M.T.
      Mol. Biol. Cell 10:2461-2474(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "The role of the TIM8-13 complex in the import of Tim23 into mitochondria."
      Paschen S.A., Rothbauer U., Kaldi K., Bauer M.F., Neupert W., Brunner M.
      EMBO J. 19:6392-6400(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria."
      Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E.
      J. Cell Biol. 150:1271-1282(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex."
      Murphy M.P., Leuenberger D., Curran S.P., Oppliger W., Koehler C.M.
      Mol. Cell. Biol. 21:6132-6138(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins."
      Curran S.P., Leuenberger D., Schmidt E., Koehler C.M.
      J. Cell Biol. 158:1017-1027(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, PROBABLE DISULFIDE BONDS, LACK OF ZINC-BINDING WHEN PRESENT IN THE MITOCHONDRIAL INTERMEMBRANE SPACE.

    Entry informationi

    Entry nameiTIM8_YEAST
    AccessioniPrimary (citable) accession number: P57744
    Secondary accession number(s): D6VWV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3