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P57744 (TIM8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM8
Gene names
Name:TIM8
Ordered Locus Names:YJR135W-A
ORF Names:YJR135BW
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length87 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIM8-TIM13 complex is non essential and only mediates the import of few proteins under precise conditions, while the predominant TIM9-TIM10 70 kDa complex is crucial and mediates the import of much more proteins. Strictly required for import of TIM23 in some conditions, when a low membrane potential exists in the mitochondria. Ref.4 Ref.6 Ref.7 Ref.8 Ref.9

Subunit structure

Heterohexamer; composed of 3 copies of TIM8 and 3 copies of TIM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54. Interacts with the transmembrane regions of multi-pass transmembrane proteins in transit. Ref.4 Ref.9

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Note: Import into inner membrane protein requires TIM5 function. Ref.4 Ref.5

Domain

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIM8 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane Probable.

Sequence similarities

Belongs to the small Tim family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TIM13P532994EBI-9013892,EBI-9121

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8787Mitochondrial import inner membrane translocase subunit TIM8
PRO_0000193594

Regions

Motif44 – 6825Twin CX3C motif

Amino acid modifications

Disulfide bond44 ↔ 68 By similarity
Disulfide bond48 ↔ 64 By similarity

Secondary structure

....... 87
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P57744 [UniParc].

Last modified January 11, 2001. Version 1.
Checksum: B91D1DD41707EA24

FASTA879,764
        10         20         30         40         50         60 
MSSLSTSDLA SLDDTSKKEI ATFLEGENSK QKVQMSIHQF TNICFKKCVE SVNDSNLSSQ 

        70         80 
EEQCLSNCVN RFLDTNIRIV NGLQNTR 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Different import pathways through the mitochondrial intermembrane space for inner membrane proteins."
Leuenberger D., Bally N.A., Schatz G., Koehler C.M.
EMBO J. 18:4816-4822(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH THE TIM22 COMPLEX.
[5]"Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway."
Kurz M., Martin H., Rassow J., Pfanner N., Ryan M.T.
Mol. Biol. Cell 10:2461-2474(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"The role of the TIM8-13 complex in the import of Tim23 into mitochondria."
Paschen S.A., Rothbauer U., Kaldi K., Bauer M.F., Neupert W., Brunner M.
EMBO J. 19:6392-6400(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Two intermembrane space TIM complexes interact with different domains of Tim23p during its import into mitochondria."
Davis A.J., Sepuri N.B., Holder J., Johnson A.E., Jensen R.E.
J. Cell Biol. 150:1271-1282(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The essential function of the small Tim proteins in the TIM22 import pathway does not depend on formation of the soluble 70-kilodalton complex."
Murphy M.P., Leuenberger D., Curran S.P., Oppliger W., Koehler C.M.
Mol. Cell. Biol. 21:6132-6138(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The role of the Tim8p-Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins."
Curran S.P., Leuenberger D., Schmidt E., Koehler C.M.
J. Cell Biol. 158:1017-1027(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, PROBABLE DISULFIDE BONDS, LACK OF ZINC-BINDING WHEN PRESENT IN THE MITOCHONDRIAL INTERMEMBRANE SPACE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49636 Genomic DNA. No translation available.
AY558258 Genomic DNA. Translation: AAS56584.1.
BK006943 Genomic DNA. Translation: DAA08920.1.
PIRS78075.
RefSeqNP_058168.1. NM_001184382.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CJHX-ray2.60B/D/F/H/J/L24-87[»]
ProteinModelPortalP57744.
SMRP57744. Positions 28-86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33891. 20 interactions.
DIPDIP-8051N.
IntActP57744. 1 interaction.
STRING4932.YJR135W-A.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBP57744.
PaxDbP57744.
PeptideAtlasP57744.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR135W-A; YJR135W-A; YJR135W-A.
GeneID853600.
KEGGsce:YJR135W-A.

Organism-specific databases

CYGDYJR135w-a.
SGDS000007348. TIM8.

Phylogenomic databases

eggNOGNOG238740.
GeneTreeENSGT00390000016102.
HOGENOMHOG000115758.
KOK17780.
OMAEINEPIR.
OrthoDBEOG7DVDQJ.

Enzyme and pathway databases

BioCycYEAST:G3O-31800-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).

Gene expression databases

GenevestigatorP57744.

Family and domain databases

Gene3D1.10.287.810. 1 hit.
InterProIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMSSF144122. SSF144122. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP57744.
NextBio974424.

Entry information

Entry nameTIM8_YEAST
AccessionPrimary (citable) accession number: P57744
Secondary accession number(s): D6VWV4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references