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Protein

Claudin-2

Gene

CLDN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-420029. Tight junction interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-2
Alternative name(s):
SP82
Gene namesi
Name:CLDN2
ORF Names:PSEC0059, SP82, UNQ705/PRO1356
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:2041. CLDN2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST
Topological domaini29 – 8153ExtracellularSequence analysisAdd
BLAST
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 11614CytoplasmicSequence analysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence analysisAdd
BLAST
Topological domaini138 – 16225ExtracellularSequence analysisAdd
BLAST
Transmembranei163 – 18321HelicalSequence analysisAdd
BLAST
Topological domaini184 – 23047CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26567.

Polymorphism and mutation databases

BioMutaiCLDN2.
DMDMi12229749.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Claudin-2PRO_0000144734Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 641 Publication
Cross-linki218 – 218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei219 – 2191PhosphoserineBy similarity
Modified residuei223 – 2231PhosphoserineBy similarity

Post-translational modificationi

The disulfide bond is necessary for pore formation, but is not required for correct protein trafficking.

Keywords - PTMi

Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP57739.
PaxDbiP57739.
PRIDEiP57739.

PTM databases

PhosphoSiteiP57739.

Expressioni

Gene expression databases

BgeeiP57739.
CleanExiHS_CLDN2.
GenevisibleiP57739. HS.

Organism-specific databases

HPAiCAB002609.
HPA030737.
HPA051548.

Interactioni

Subunit structurei

Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN1, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT31Q153233EBI-751440,EBI-948001
NOTCH2NLQ7Z3S93EBI-751440,EBI-945833

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114532. 9 interactions.
IntActiP57739. 4 interactions.
MINTiMINT-1476912.
STRINGi9606.ENSP00000336571.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YYXX-ray1.79A/B224-230[»]
ProteinModelPortaliP57739.
SMRiP57739. Positions 5-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2302Interactions with TJP1, TJP2 and TJP3By similarity

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG06. Eukaryota.
ENOG410YBJ7. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiP57739.
KOiK06087.
OMAiRTSSYVG.
OrthoDBiEOG76HQ2T.
PhylomeDBiP57739.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR005411. Claudin2.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01589. CLAUDIN2.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLGLQLVG YILGLLGLLG TLVAMLLPSW KTSSYVGASI VTAVGFSKGL
60 70 80 90 100
WMECATHSTG ITQCDIYSTL LGLPADIQAA QAMMVTSSAI SSLACIISVV
110 120 130 140 150
GMRCTVFCQE SRAKDRVAVA GGVFFILGGL LGFIPVAWNL HGILRDFYSP
160 170 180 190 200
LVPDSMKFEI GEALYLGIIS SLFSLIAGII LCFSCSSQRN RSNYYDAYQA
210 220 230
QPLATRSSPR PGQPPKVKSE FNSYSLTGYV
Length:230
Mass (Da):24,549
Last modified:January 11, 2001 - v1
Checksum:i52CA642D4A62B70D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF250558 mRNA. Translation: AAF98151.1.
AY358474 mRNA. Translation: AAQ88838.1.
AK312515 mRNA. Translation: BAG35416.1.
AF177340 mRNA. Translation: AAG17984.1.
AK075371 mRNA. Translation: BAC11575.1.
AK075405 mRNA. Translation: BAG52130.1.
AL158821 Genomic DNA. Translation: CAD23055.1.
CH471120 Genomic DNA. Translation: EAX02730.1.
CH471120 Genomic DNA. Translation: EAX02731.1.
BC014424 mRNA. Translation: AAH14424.1.
BC071747 mRNA. Translation: AAH71747.1.
CCDSiCCDS14524.1.
RefSeqiNP_001164563.1. NM_001171092.1.
NP_001164566.1. NM_001171095.1.
NP_065117.1. NM_020384.3.
UniGeneiHs.522746.

Genome annotation databases

EnsembliENST00000336803; ENSP00000336571; ENSG00000165376.
ENST00000540876; ENSP00000443230; ENSG00000165376.
ENST00000541806; ENSP00000441283; ENSG00000165376.
GeneIDi9075.
KEGGihsa:9075.
UCSCiuc004emq.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF250558 mRNA. Translation: AAF98151.1.
AY358474 mRNA. Translation: AAQ88838.1.
AK312515 mRNA. Translation: BAG35416.1.
AF177340 mRNA. Translation: AAG17984.1.
AK075371 mRNA. Translation: BAC11575.1.
AK075405 mRNA. Translation: BAG52130.1.
AL158821 Genomic DNA. Translation: CAD23055.1.
CH471120 Genomic DNA. Translation: EAX02730.1.
CH471120 Genomic DNA. Translation: EAX02731.1.
BC014424 mRNA. Translation: AAH14424.1.
BC071747 mRNA. Translation: AAH71747.1.
CCDSiCCDS14524.1.
RefSeqiNP_001164563.1. NM_001171092.1.
NP_001164566.1. NM_001171095.1.
NP_065117.1. NM_020384.3.
UniGeneiHs.522746.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YYXX-ray1.79A/B224-230[»]
ProteinModelPortaliP57739.
SMRiP57739. Positions 5-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114532. 9 interactions.
IntActiP57739. 4 interactions.
MINTiMINT-1476912.
STRINGi9606.ENSP00000336571.

PTM databases

PhosphoSiteiP57739.

Polymorphism and mutation databases

BioMutaiCLDN2.
DMDMi12229749.

Proteomic databases

MaxQBiP57739.
PaxDbiP57739.
PRIDEiP57739.

Protocols and materials databases

DNASUi9075.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336803; ENSP00000336571; ENSG00000165376.
ENST00000540876; ENSP00000443230; ENSG00000165376.
ENST00000541806; ENSP00000441283; ENSG00000165376.
GeneIDi9075.
KEGGihsa:9075.
UCSCiuc004emq.2. human.

Organism-specific databases

CTDi9075.
GeneCardsiCLDN2.
HGNCiHGNC:2041. CLDN2.
HPAiCAB002609.
HPA030737.
HPA051548.
MIMi300520. gene.
neXtProtiNX_P57739.
PharmGKBiPA26567.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG06. Eukaryota.
ENOG410YBJ7. LUCA.
GeneTreeiENSGT00760000118928.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiP57739.
KOiK06087.
OMAiRTSSYVG.
OrthoDBiEOG76HQ2T.
PhylomeDBiP57739.
TreeFamiTF331936.

Enzyme and pathway databases

ReactomeiR-HSA-420029. Tight junction interactions.

Miscellaneous databases

GeneWikiiCLDN2.
GenomeRNAii9075.
NextBioi34005.
PROiP57739.
SOURCEiSearch...

Gene expression databases

BgeeiP57739.
CleanExiHS_CLDN2.
GenevisibleiP57739. HS.

Family and domain databases

InterProiIPR006187. Claudin.
IPR005411. Claudin2.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01589. CLAUDIN2.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the human claudin-2 5'-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1alpha."
    Sakaguchi T., Gu X., Golden H.M., Suh E., Rhoads D.B., Reinecker H.-C.
    J. Biol. Chem. 277:21361-21370(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Colon epithelium.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thalamus.
  4. "Large-scale cDNA transfection screening for genes related to cancer development and progression."
    Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.
    , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.
    Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Teratocarcinoma.
  6. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon and Skin.
  9. Cited for: SUMOYLATION AT LYS-218.
  10. "Claudin-2 pore function requires an intramolecular disulfide bond between two conserved extracellular cysteines."
    Li J., Angelow S., Linge A., Zhuo M., Yu A.S.
    Am. J. Physiol. 305:C190-C196(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BOND.

Entry informationi

Entry nameiCLD2_HUMAN
AccessioniPrimary (citable) accession number: P57739
Secondary accession number(s): B2R6B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: March 16, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.