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P57735

- RAB25_HUMAN

UniProt

P57735 - RAB25_HUMAN

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Protein

Ras-related protein Rab-25

Gene

RAB25

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279GTP
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi125 – 1284GTP
Nucleotide bindingi155 – 1573GTP

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. myosin V binding Source: UniProtKB

GO - Biological processi

  1. positive regulation of cell proliferation Source: UniProtKB
  2. protein transport Source: UniProtKB-KW
  3. pseudopodium organization Source: UniProtKB
  4. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-25
Alternative name(s):
CATX-8
Gene namesi
Name:RAB25
Synonyms:CATX8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:18238. RAB25.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Cell projectionpseudopodium membrane. Cytoplasmic vesicle
Note: Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProt
  3. plasma membrane Source: UniProtKB-KW
  4. pseudopodium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34115.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Ras-related protein Rab-25PRO_0000121215Add
BLAST
Propeptidei211 – 2133Removed in mature formSequence AnalysisPRO_0000370821

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi209 – 2091S-geranylgeranyl cysteineBy similarity
Modified residuei210 – 2101Cysteine methyl esterSequence Analysis
Lipidationi210 – 2101S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP57735.
PaxDbiP57735.
PRIDEiP57735.

PTM databases

PhosphoSiteiP57735.

Expressioni

Tissue specificityi

Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.1 Publication

Gene expression databases

BgeeiP57735.
CleanExiHS_RAB25.
GenevestigatoriP57735.

Organism-specific databases

HPAiHPA010872.

Interactioni

Subunit structurei

Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer.3 Publications

Protein-protein interaction databases

BioGridi121377. 14 interactions.
STRINGi9606.ENSP00000354376.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 199Combined sources
Helixi25 – 3410Combined sources
Beta strandi46 – 5611Combined sources
Beta strandi59 – 6810Combined sources
Helixi78 – 825Combined sources
Beta strandi87 – 937Combined sources
Helixi97 – 1015Combined sources
Helixi103 – 1119Combined sources
Beta strandi119 – 1257Combined sources
Helixi127 – 1326Combined sources
Helixi137 – 14610Combined sources
Beta strandi150 – 1534Combined sources
Turni156 – 1583Combined sources
Helixi162 – 17716Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OILX-ray2.30A7-180[»]
3TSOX-ray1.80A/B7-180[»]
ProteinModelPortaliP57735.
SMRiP57735. Positions 10-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP57735.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 499Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP57735.
KOiK07906.
OMAiMEEAKMF.
OrthoDBiEOG7SFHZ2.
PhylomeDBiP57735.
TreeFamiTF300099.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57735-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNGTEEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS
60 70 80 90 100
TRTVMLGTAA VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY
110 120 130 140 150
AVVERWLKEL YDHAEATIVV MLVGNKSDLS QAREVPTEEA RMFAENNGLL
160 170 180 190 200
FLETSALDST NVELAFETVL KEIFAKVSKQ RQNSIRTNAI TLGSAQAGQE
210
PGPGEKRACC ISL
Length:213
Mass (Da):23,496
Last modified:April 26, 2004 - v2
Checksum:i119523C3036C370E
GO

Sequence cautioni

The sequence AAF98238.1 differs from that shown. Reason: Frameshift at position 196.
The sequence AAM69362.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031P → L in AAF98238. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF083124 mRNA. Translation: AAF98238.1. Frameshift.
AF274025 mRNA. Translation: AAM69362.1. Different initiation.
AL355388 Genomic DNA. Translation: CAH72638.1.
CH471121 Genomic DNA. Translation: EAW53003.1.
BC004416 mRNA. Translation: AAH04416.1.
BC009831 mRNA. Translation: AAH09831.1.
BC033322 mRNA. Translation: AAH33322.1.
CCDSiCCDS41413.1.
RefSeqiNP_065120.2. NM_020387.2.
UniGeneiHs.632469.

Genome annotation databases

EnsembliENST00000361084; ENSP00000354376; ENSG00000132698.
ENST00000615862; ENSP00000482161; ENSG00000132698.
GeneIDi57111.
KEGGihsa:57111.
UCSCiuc001fnc.3. human.

Polymorphism databases

DMDMi46577696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF083124 mRNA. Translation: AAF98238.1 . Frameshift.
AF274025 mRNA. Translation: AAM69362.1 . Different initiation.
AL355388 Genomic DNA. Translation: CAH72638.1 .
CH471121 Genomic DNA. Translation: EAW53003.1 .
BC004416 mRNA. Translation: AAH04416.1 .
BC009831 mRNA. Translation: AAH09831.1 .
BC033322 mRNA. Translation: AAH33322.1 .
CCDSi CCDS41413.1.
RefSeqi NP_065120.2. NM_020387.2.
UniGenei Hs.632469.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OIL X-ray 2.30 A 7-180 [» ]
3TSO X-ray 1.80 A/B 7-180 [» ]
ProteinModelPortali P57735.
SMRi P57735. Positions 10-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121377. 14 interactions.
STRINGi 9606.ENSP00000354376.

PTM databases

PhosphoSitei P57735.

Polymorphism databases

DMDMi 46577696.

Proteomic databases

MaxQBi P57735.
PaxDbi P57735.
PRIDEi P57735.

Protocols and materials databases

DNASUi 57111.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000361084 ; ENSP00000354376 ; ENSG00000132698 .
ENST00000615862 ; ENSP00000482161 ; ENSG00000132698 .
GeneIDi 57111.
KEGGi hsa:57111.
UCSCi uc001fnc.3. human.

Organism-specific databases

CTDi 57111.
GeneCardsi GC01P156030.
HGNCi HGNC:18238. RAB25.
HPAi HPA010872.
MIMi 612942. gene.
neXtProti NX_P57735.
PharmGKBi PA34115.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118841.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi P57735.
KOi K07906.
OMAi MEEAKMF.
OrthoDBi EOG7SFHZ2.
PhylomeDBi P57735.
TreeFami TF300099.

Miscellaneous databases

EvolutionaryTracei P57735.
GeneWikii RAB25.
GenomeRNAii 57111.
NextBioi 62967.
PROi P57735.
SOURCEi Search...

Gene expression databases

Bgeei P57735.
CleanExi HS_RAB25.
Genevestigatori P57735.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of novel genes from human colonic epithelial cells."
    Wang L., Kairo A., Gao Z.Q., Gao Z.P., Boman B.M.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Colon.
  2. Yu L.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary, Pancreas and Skin.
  6. Cited for: INTERACTION WITH RAB11FIP1; RAB11FIP2; RAB11FIP3 AND RAB11FIP4.
  7. "The RAB25 small GTPase determines aggressiveness of ovarian and breast cancers."
    Cheng K.W., Lahad J.P., Kuo W.L., Lapuk A., Yamada K., Auersperg N., Liu J., Smith-McCune K., Lu K.H., Fishman D., Gray J.W., Mills G.B.
    Nat. Med. 10:1251-1256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  8. "Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments."
    Caswell P.T., Spence H.J., Parsons M., White D.P., Clark K., Cheng K.W., Mills G.B., Humphries M.J., Messent A.J., Anderson K.I., McCaffrey M.W., Ozanne B.W., Norman J.C.
    Dev. Cell 13:496-510(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ITGAV AND ITGB1.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Crystal structure of human RAB25 in complex with GDP."
    Structural genomics consortium (SGC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 7-180 IN COMPLEX WITH GDP.

Entry informationi

Entry nameiRAB25_HUMAN
AccessioniPrimary (citable) accession number: P57735
Secondary accession number(s): Q5VYA2
, Q8NG24, Q96GB1, Q9BT12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 26, 2004
Last modified: October 29, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3