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Protein

Ras-related protein Rab-25

Gene

RAB25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia (PubMed:17925226). Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (By similarity). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27GTP9
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi125 – 128GTP4
Nucleotide bindingi155 – 157GTP3

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • myosin V binding Source: UniProtKB

GO - Biological processi

  • epithelial cell morphogenesis Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of epithelial cell migration Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • pseudopodium organization Source: UniProtKB
  • regulation of vesicle-mediated transport Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132698-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-25
Alternative name(s):
CATX-8
Gene namesi
Name:RAB25Imported
Synonyms:CATX8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:18238. RAB25.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytoplasmic vesicle Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • pseudopodium Source: UniProtKB
  • pseudopodium membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57111.
OpenTargetsiENSG00000132698.
PharmGKBiPA34115.

Polymorphism and mutation databases

BioMutaiRAB25.
DMDMi46577696.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001212151 – 210Ras-related protein Rab-25Add BLAST210
PropeptideiPRO_0000370821211 – 213Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi209S-geranylgeranyl cysteineBy similarity1
Modified residuei210Cysteine methyl esterSequence analysis1
Lipidationi210S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP57735.
MaxQBiP57735.
PaxDbiP57735.
PeptideAtlasiP57735.
PRIDEiP57735.

PTM databases

iPTMnetiP57735.
PhosphoSitePlusiP57735.

Expressioni

Tissue specificityi

Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.1 Publication

Gene expression databases

BgeeiENSG00000132698.
CleanExiHS_RAB25.
GenevisibleiP57735. HS.

Organism-specific databases

HPAiHPA010872.

Interactioni

Subunit structurei

Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer.3 Publications

GO - Molecular functioni

  • myosin V binding Source: UniProtKB

Protein-protein interaction databases

BioGridi121377. 17 interactors.
IntActiP57735. 1 interactor.
STRINGi9606.ENSP00000354376.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 19Combined sources9
Helixi25 – 34Combined sources10
Beta strandi46 – 56Combined sources11
Beta strandi59 – 68Combined sources10
Helixi78 – 82Combined sources5
Beta strandi87 – 93Combined sources7
Helixi97 – 101Combined sources5
Helixi103 – 111Combined sources9
Beta strandi119 – 125Combined sources7
Helixi127 – 132Combined sources6
Helixi137 – 146Combined sources10
Beta strandi150 – 153Combined sources4
Turni156 – 158Combined sources3
Helixi162 – 177Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OILX-ray2.30A7-180[»]
3TSOX-ray1.80A/B7-180[»]
ProteinModelPortaliP57735.
SMRiP57735.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP57735.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi41 – 49Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0087. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP57735.
KOiK07906.
PhylomeDBiP57735.
TreeFamiTF300099.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P57735-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNGTEEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS
60 70 80 90 100
TRTVMLGTAA VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY
110 120 130 140 150
AVVERWLKEL YDHAEATIVV MLVGNKSDLS QAREVPTEEA RMFAENNGLL
160 170 180 190 200
FLETSALDST NVELAFETVL KEIFAKVSKQ RQNSIRTNAI TLGSAQAGQE
210
PGPGEKRACC ISL
Length:213
Mass (Da):23,496
Last modified:April 26, 2004 - v2
Checksum:i119523C3036C370E
GO

Sequence cautioni

The sequence AAF98238 differs from that shown. Reason: Frameshift at position 196.Curated
The sequence AAM69362 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203P → L in AAF98238 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083124 mRNA. Translation: AAF98238.1. Frameshift.
AF274025 mRNA. Translation: AAM69362.1. Different initiation.
AL355388 Genomic DNA. Translation: CAH72638.1.
CH471121 Genomic DNA. Translation: EAW53003.1.
BC004416 mRNA. Translation: AAH04416.1.
BC009831 mRNA. Translation: AAH09831.1.
BC033322 mRNA. Translation: AAH33322.1.
CCDSiCCDS41413.1.
RefSeqiNP_065120.2. NM_020387.3.
UniGeneiHs.632469.

Genome annotation databases

EnsembliENST00000361084; ENSP00000354376; ENSG00000132698.
GeneIDi57111.
KEGGihsa:57111.
UCSCiuc001fnc.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083124 mRNA. Translation: AAF98238.1. Frameshift.
AF274025 mRNA. Translation: AAM69362.1. Different initiation.
AL355388 Genomic DNA. Translation: CAH72638.1.
CH471121 Genomic DNA. Translation: EAW53003.1.
BC004416 mRNA. Translation: AAH04416.1.
BC009831 mRNA. Translation: AAH09831.1.
BC033322 mRNA. Translation: AAH33322.1.
CCDSiCCDS41413.1.
RefSeqiNP_065120.2. NM_020387.3.
UniGeneiHs.632469.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OILX-ray2.30A7-180[»]
3TSOX-ray1.80A/B7-180[»]
ProteinModelPortaliP57735.
SMRiP57735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121377. 17 interactors.
IntActiP57735. 1 interactor.
STRINGi9606.ENSP00000354376.

PTM databases

iPTMnetiP57735.
PhosphoSitePlusiP57735.

Polymorphism and mutation databases

BioMutaiRAB25.
DMDMi46577696.

Proteomic databases

EPDiP57735.
MaxQBiP57735.
PaxDbiP57735.
PeptideAtlasiP57735.
PRIDEiP57735.

Protocols and materials databases

DNASUi57111.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361084; ENSP00000354376; ENSG00000132698.
GeneIDi57111.
KEGGihsa:57111.
UCSCiuc001fnc.4. human.

Organism-specific databases

CTDi57111.
DisGeNETi57111.
GeneCardsiRAB25.
HGNCiHGNC:18238. RAB25.
HPAiHPA010872.
MIMi612942. gene.
neXtProtiNX_P57735.
OpenTargetsiENSG00000132698.
PharmGKBiPA34115.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0087. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP57735.
KOiK07906.
PhylomeDBiP57735.
TreeFamiTF300099.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132698-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP57735.
GeneWikiiRAB25.
GenomeRNAii57111.
PROiP57735.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132698.
CleanExiHS_RAB25.
GenevisibleiP57735. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB25_HUMAN
AccessioniPrimary (citable) accession number: P57735
Secondary accession number(s): Q5VYA2
, Q8NG24, Q96GB1, Q9BT12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 26, 2004
Last modified: November 30, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.