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P57735 (RAB25_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-25
Alternative name(s):
CATX-8
Gene names
Name:RAB25
Synonyms:CATX8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis By similarity. Ref.7 Ref.8

Subunit structure

Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Ref.6 Ref.8

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cell projectionpseudopodium membrane. Cytoplasmic vesicle. Note: Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.

Tissue specificity

Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue. Ref.7

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence caution

The sequence AAF98238.1 differs from that shown. Reason: Frameshift at position 196.

The sequence AAM69362.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Ras-related protein Rab-25
PRO_0000121215
Propeptide211 – 2133Removed in mature form Potential
PRO_0000370821

Regions

Nucleotide binding19 – 279GTP
Nucleotide binding67 – 715GTP By similarity
Nucleotide binding125 – 1284GTP
Nucleotide binding155 – 1573GTP
Motif41 – 499Effector region By similarity

Amino acid modifications

Modified residue2101Cysteine methyl ester Potential
Lipidation2091S-geranylgeranyl cysteine By similarity
Lipidation2101S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict2031P → L in AAF98238. Ref.1

Secondary structure

............................. 213
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P57735 [UniParc].

Last modified April 26, 2004. Version 2.
Checksum: 119523C3036C370E

FASTA21323,496
        10         20         30         40         50         60 
MGNGTEEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS TRTVMLGTAA 

        70         80         90        100        110        120 
VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY AVVERWLKEL YDHAEATIVV 

       130        140        150        160        170        180 
MLVGNKSDLS QAREVPTEEA RMFAENNGLL FLETSALDST NVELAFETVL KEIFAKVSKQ 

       190        200        210 
RQNSIRTNAI TLGSAQAGQE PGPGEKRACC ISL 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of novel genes from human colonic epithelial cells."
Wang L., Kairo A., Gao Z.Q., Gao Z.P., Boman B.M.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Colon.
[2]Yu L.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Ovary, Pancreas and Skin.
[6]"Identification and characterization of a family of Rab11-interacting proteins."
Hales C.M., Griner R., Hobdy-Henderson K.C., Dorn M.C., Hardy D., Kumar R., Navarre J., Chan E.K.L., Lapierre L.A., Goldenring J.R.
J. Biol. Chem. 276:39067-39075(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RAB11FIP1; RAB11FIP2; RAB11FIP3 AND RAB11FIP4.
[7]"The RAB25 small GTPase determines aggressiveness of ovarian and breast cancers."
Cheng K.W., Lahad J.P., Kuo W.L., Lapuk A., Yamada K., Auersperg N., Liu J., Smith-McCune K., Lu K.H., Fishman D., Gray J.W., Mills G.B.
Nat. Med. 10:1251-1256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[8]"Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments."
Caswell P.T., Spence H.J., Parsons M., White D.P., Clark K., Cheng K.W., Mills G.B., Humphries M.J., Messent A.J., Anderson K.I., McCaffrey M.W., Ozanne B.W., Norman J.C.
Dev. Cell 13:496-510(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ITGAV AND ITGB1.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Crystal structure of human RAB25 in complex with GDP."
Structural genomics consortium (SGC)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 7-180 IN COMPLEX WITH GDP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF083124 mRNA. Translation: AAF98238.1. Frameshift.
AF274025 mRNA. Translation: AAM69362.1. Different initiation.
AL355388 Genomic DNA. Translation: CAH72638.1.
CH471121 Genomic DNA. Translation: EAW53003.1.
BC004416 mRNA. Translation: AAH04416.1.
BC009831 mRNA. Translation: AAH09831.1.
BC033322 mRNA. Translation: AAH33322.1.
CCDSCCDS41413.1.
RefSeqNP_065120.2. NM_020387.2.
UniGeneHs.632469.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2OILX-ray2.30A7-180[»]
3TSOX-ray1.80A/B7-180[»]
ProteinModelPortalP57735.
SMRP57735. Positions 10-178.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121377. 12 interactions.
STRING9606.ENSP00000354376.

PTM databases

PhosphoSiteP57735.

Polymorphism databases

DMDM46577696.

Proteomic databases

MaxQBP57735.
PaxDbP57735.
PRIDEP57735.

Protocols and materials databases

DNASU57111.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361084; ENSP00000354376; ENSG00000132698.
GeneID57111.
KEGGhsa:57111.
UCSCuc001fnc.3. human.

Organism-specific databases

CTD57111.
GeneCardsGC01P156030.
HGNCHGNC:18238. RAB25.
HPAHPA010872.
MIM612942. gene.
neXtProtNX_P57735.
PharmGKBPA34115.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP57735.
KOK07906.
OMAMEEAKMF.
OrthoDBEOG7SFHZ2.
PhylomeDBP57735.
TreeFamTF300099.

Gene expression databases

BgeeP57735.
CleanExHS_RAB25.
GenevestigatorP57735.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP57735.
GeneWikiRAB25.
GenomeRNAi57111.
NextBio62967.
PROP57735.
SOURCESearch...

Entry information

Entry nameRAB25_HUMAN
AccessionPrimary (citable) accession number: P57735
Secondary accession number(s): Q5VYA2 expand/collapse secondary AC list , Q8NG24, Q96GB1, Q9BT12
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: April 26, 2004
Last modified: July 9, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM