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P57735

- RAB25_HUMAN

UniProt

P57735 - RAB25_HUMAN

Protein

Ras-related protein Rab-25

Gene

RAB25

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 2 (26 Apr 2004)
      Previous versions | rss
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    Functioni

    Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 279GTP
    Nucleotide bindingi67 – 715GTPBy similarity
    Nucleotide bindingi125 – 1284GTP
    Nucleotide bindingi155 – 1573GTP

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. positive regulation of cell proliferation Source: UniProtKB
    2. protein transport Source: UniProtKB-KW
    3. pseudopodium organization Source: UniProtKB
    4. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-25
    Alternative name(s):
    CATX-8
    Gene namesi
    Name:RAB25
    Synonyms:CATX8
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:18238. RAB25.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Cell projectionpseudopodium membrane. Cytoplasmic vesicle
    Note: Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    2. cytoplasmic vesicle Source: UniProtKB
    3. extracellular vesicular exosome Source: UniProt
    4. pseudopodium Source: UniProtKB
    5. pseudopodium membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34115.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 210210Ras-related protein Rab-25PRO_0000121215Add
    BLAST
    Propeptidei211 – 2133Removed in mature formSequence AnalysisPRO_0000370821

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi209 – 2091S-geranylgeranyl cysteineBy similarity
    Modified residuei210 – 2101Cysteine methyl esterSequence Analysis
    Lipidationi210 – 2101S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiP57735.
    PaxDbiP57735.
    PRIDEiP57735.

    PTM databases

    PhosphoSiteiP57735.

    Expressioni

    Tissue specificityi

    Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.1 Publication

    Gene expression databases

    BgeeiP57735.
    CleanExiHS_RAB25.
    GenevestigatoriP57735.

    Organism-specific databases

    HPAiHPA010872.

    Interactioni

    Subunit structurei

    Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer.3 Publications

    Protein-protein interaction databases

    BioGridi121377. 12 interactions.
    STRINGi9606.ENSP00000354376.

    Structurei

    Secondary structure

    1
    213
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi11 – 199
    Helixi25 – 3410
    Beta strandi46 – 5611
    Beta strandi59 – 6810
    Helixi78 – 825
    Beta strandi87 – 937
    Helixi97 – 1015
    Helixi103 – 1119
    Beta strandi119 – 1257
    Helixi127 – 1326
    Helixi137 – 14610
    Beta strandi150 – 1534
    Turni156 – 1583
    Helixi162 – 17716

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OILX-ray2.30A7-180[»]
    3TSOX-ray1.80A/B7-180[»]
    ProteinModelPortaliP57735.
    SMRiP57735. Positions 10-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP57735.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi41 – 499Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP57735.
    KOiK07906.
    OMAiMEEAKMF.
    OrthoDBiEOG7SFHZ2.
    PhylomeDBiP57735.
    TreeFamiTF300099.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P57735-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNGTEEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS    50
    TRTVMLGTAA VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY 100
    AVVERWLKEL YDHAEATIVV MLVGNKSDLS QAREVPTEEA RMFAENNGLL 150
    FLETSALDST NVELAFETVL KEIFAKVSKQ RQNSIRTNAI TLGSAQAGQE 200
    PGPGEKRACC ISL 213
    Length:213
    Mass (Da):23,496
    Last modified:April 26, 2004 - v2
    Checksum:i119523C3036C370E
    GO

    Sequence cautioni

    The sequence AAF98238.1 differs from that shown. Reason: Frameshift at position 196.
    The sequence AAM69362.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti203 – 2031P → L in AAF98238. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083124 mRNA. Translation: AAF98238.1. Frameshift.
    AF274025 mRNA. Translation: AAM69362.1. Different initiation.
    AL355388 Genomic DNA. Translation: CAH72638.1.
    CH471121 Genomic DNA. Translation: EAW53003.1.
    BC004416 mRNA. Translation: AAH04416.1.
    BC009831 mRNA. Translation: AAH09831.1.
    BC033322 mRNA. Translation: AAH33322.1.
    CCDSiCCDS41413.1.
    RefSeqiNP_065120.2. NM_020387.2.
    UniGeneiHs.632469.

    Genome annotation databases

    EnsembliENST00000361084; ENSP00000354376; ENSG00000132698.
    GeneIDi57111.
    KEGGihsa:57111.
    UCSCiuc001fnc.3. human.

    Polymorphism databases

    DMDMi46577696.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083124 mRNA. Translation: AAF98238.1 . Frameshift.
    AF274025 mRNA. Translation: AAM69362.1 . Different initiation.
    AL355388 Genomic DNA. Translation: CAH72638.1 .
    CH471121 Genomic DNA. Translation: EAW53003.1 .
    BC004416 mRNA. Translation: AAH04416.1 .
    BC009831 mRNA. Translation: AAH09831.1 .
    BC033322 mRNA. Translation: AAH33322.1 .
    CCDSi CCDS41413.1.
    RefSeqi NP_065120.2. NM_020387.2.
    UniGenei Hs.632469.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OIL X-ray 2.30 A 7-180 [» ]
    3TSO X-ray 1.80 A/B 7-180 [» ]
    ProteinModelPortali P57735.
    SMRi P57735. Positions 10-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121377. 12 interactions.
    STRINGi 9606.ENSP00000354376.

    PTM databases

    PhosphoSitei P57735.

    Polymorphism databases

    DMDMi 46577696.

    Proteomic databases

    MaxQBi P57735.
    PaxDbi P57735.
    PRIDEi P57735.

    Protocols and materials databases

    DNASUi 57111.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000361084 ; ENSP00000354376 ; ENSG00000132698 .
    GeneIDi 57111.
    KEGGi hsa:57111.
    UCSCi uc001fnc.3. human.

    Organism-specific databases

    CTDi 57111.
    GeneCardsi GC01P156030.
    HGNCi HGNC:18238. RAB25.
    HPAi HPA010872.
    MIMi 612942. gene.
    neXtProti NX_P57735.
    PharmGKBi PA34115.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P57735.
    KOi K07906.
    OMAi MEEAKMF.
    OrthoDBi EOG7SFHZ2.
    PhylomeDBi P57735.
    TreeFami TF300099.

    Miscellaneous databases

    EvolutionaryTracei P57735.
    GeneWikii RAB25.
    GenomeRNAii 57111.
    NextBioi 62967.
    PROi P57735.
    SOURCEi Search...

    Gene expression databases

    Bgeei P57735.
    CleanExi HS_RAB25.
    Genevestigatori P57735.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of novel genes from human colonic epithelial cells."
      Wang L., Kairo A., Gao Z.Q., Gao Z.P., Boman B.M.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Colon.
    2. Yu L.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary, Pancreas and Skin.
    6. Cited for: INTERACTION WITH RAB11FIP1; RAB11FIP2; RAB11FIP3 AND RAB11FIP4.
    7. "The RAB25 small GTPase determines aggressiveness of ovarian and breast cancers."
      Cheng K.W., Lahad J.P., Kuo W.L., Lapuk A., Yamada K., Auersperg N., Liu J., Smith-McCune K., Lu K.H., Fishman D., Gray J.W., Mills G.B.
      Nat. Med. 10:1251-1256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    8. "Rab25 associates with alpha5beta1 integrin to promote invasive migration in 3D microenvironments."
      Caswell P.T., Spence H.J., Parsons M., White D.P., Clark K., Cheng K.W., Mills G.B., Humphries M.J., Messent A.J., Anderson K.I., McCaffrey M.W., Ozanne B.W., Norman J.C.
      Dev. Cell 13:496-510(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ITGAV AND ITGB1.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Crystal structure of human RAB25 in complex with GDP."
      Structural genomics consortium (SGC)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 7-180 IN COMPLEX WITH GDP.

    Entry informationi

    Entry nameiRAB25_HUMAN
    AccessioniPrimary (citable) accession number: P57735
    Secondary accession number(s): Q5VYA2
    , Q8NG24, Q96GB1, Q9BT12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: April 26, 2004
    Last modified: October 1, 2014
    This is version 133 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3