P57730 (CAR18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caspase recruitment domain-containing protein 18

Alternative name(s):
Caspase-1 inhibitor Iceberg

Gene names

Name:	CARD18
Synonyms:	ICEBERG
ORF Names:	UNQ5804/PRO19611

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits generation of IL-1-beta by interacting with caspase-1 and preventing its association with RIP2. Down-regulates the release of IL1B. Ref.1
Subunit structure	Interacts with pro-CASP1. Interacts with CARD8. Ref.1 Ref.4
Tissue specificity	Primarily expressed in the heart and placenta. Ref.1
Induction	Up-regulated in response to TNF and bacterial lipopolysaccharides (LPS). Ref.1
Sequence similarities	Contains 1 CARD domain.

Ontologies

Keywords
Molecular function	Protease inhibitor Thiol protease inhibitor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inflammatory response Non-traceable author statement Ref.1. Source: UniProtKB regulation of apoptotic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 90

90

Caspase recruitment domain-containing protein 18

PRO_0000084145

Regions

Domain

1 – 90

90

CARD

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

90

Helix Strand Turn

Details...

Helix

5 – 8

4

Helix

10 – 14

5

Helix

19 – 32

14

Helix

37 – 44

8

Helix

50 – 63

14

Helix

66 – 79

14

Helix

83 – 87

5

Sequences

	Sequence		Length	Mass (Da)
	P57730 [UniParc]. Last modified January 11, 2001. Version 1. Checksum: 7E74C74263367101	FASTA	90	10,138
	10 20 30 40 50 60 MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL 70 80 90 VTGKGPKSCC KFIKHLCEED PQLASKMGLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ICEBERG: a novel inhibitor of interleukin-1beta generation." Humke E.W., Shriver S.K., Starovasnik M.A., Fairbrother W.J., Dixit V.M. Cell 103:99-111(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], STRUCTURE BY NMR, FUNCTION, INTERACTION WITH CASP1, INDUCTION, TISSUE SPECIFICITY.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis." Razmara M., Srinivasula S.M., Wang L., Poyet J.-L., Geddes B.J., DiStefano P.S., Bertin J., Alnemri E.S. J. Biol. Chem. 277:13952-13958(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CARD8.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF208005 mRNA. Translation: AAG23528.1.
AY358231 mRNA. Translation: AAQ88598.1.
BC131610 mRNA. Translation: AAI31611.1.

IPI

IPI00027987.

RefSeq

NP_067546.1. NM_021571.3.

UniGene

Hs.56279.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DGN	NMR	-	A	2-90	[»]

ProteinModelPortal

P57730.

ModBase

Search...

PTM databases

PhosphoSite

P57730.

Polymorphism databases

DMDM

12230142.

Proteomic databases

PRIDE

P57730.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000530950; ENSP00000436691; ENSG00000255501.

GeneID

59082.

KEGG

hsa:59082.

UCSC

uc021qpy.1. human.

Organism-specific databases

CTD

59082.

GeneCards

GC11M105008.

HGNC

HGNC:28861. CARD18.

HPA

HPA038582.

MIM

605354. gene.

neXtProt

NX_P57730.

PharmGKB

PA164717642.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG006113.

InParanoid

P57730.

KO

K12806.

OMA

ISQEDMN.

Gene expression databases

ArrayExpress

P57730.

Bgee

P57730.

CleanEx

HS_CARD18.

Genevestigator

P57730.

GermOnline

ENSG00000118168. Homo sapiens.

Family and domain databases

Gene3D

1.10.533.10. 1 hit.

InterPro

IPR001315. CARD.
IPR011029. DEATH-like_dom.
[Graphical view]

Pfam

PF00619. CARD. 1 hit.
[Graphical view]

SMART

SM00114. CARD. 1 hit.
[Graphical view]

SUPFAM

SSF47986. DEATH_like. 1 hit.

PROSITE

PS50209. CARD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P57730.

GenomeRNAi

59082.

NextBio

65139.

SOURCE

Search...

Entry information

Entry name

CAR18_HUMAN

Accession

Primary (citable) accession number: P57730
Secondary accession number(s): A2RRF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.