Reviewed, UniProtKB/Swiss-Prot P57730 (CAR18_HUMAN)

Names and origin

Protein names

Recommended name:
Caspase recruitment domain-containing protein 18

Alternative name(s):
Caspase-1 inhibitor Iceberg

Gene names

Name:	CARD18
Synonyms:	ICEBERG
ORF Names:	UNQ5804/PRO19611

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits generation of IL-1-beta by interacting with caspase-1 and preventing its association with RIP2. Down-regulates the release of IL1B. Ref.1
Subunit structure	Interacts with pro-CASP1. Interacts with CARD8. Ref.1 Ref.4
Tissue specificity	Primarily expressed in the heart and placenta. Ref.1
Induction	Up-regulated in response to TNF and bacterial lipopolysaccharide (LPS). Ref.1
Sequence similarities	Contains 1 CARD domain.

Ontologies

Keywords
Molecular function	Protease inhibitor Thiol protease inhibitor
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	inflammatory response Ref.1 Non-traceable author statement. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: InterPro regulation of apoptosis Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro cysteine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 90

90

Caspase recruitment domain-containing protein 18

PRO_0000084145

Regions

Domain

1 – 90

90

CARD

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

90

Helix Strand Turn

Details...

Helix

5 – 8

4

Helix

10 – 14

5

Helix

19 – 32

14

Helix

37 – 44

8

Helix

50 – 63

14

Helix

66 – 79

14

Helix

83 – 87

5

Sequences

	Sequence		Length	Mass (Da)
	P57730-1 [UniParc]. Last modified January 11, 2001. Version 1. Checksum: 7E74C74263367101	FASTA	90	10,138
	10 20 30 40 50 60 MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL 70 80 90 VTGKGPKSCC KFIKHLCEED PQLASKMGLH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ICEBERG: a novel inhibitor of interleukin-1beta generation." Humke E.W., Shriver S.K., Starovasnik M.A., Fairbrother W.J., Dixit V.M. Cell 103:99-111(2000) [PubMed: 11051551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], STRUCTURE BY NMR, FUNCTION, INTERACTION WITH CASP1, INDUCTION, TISSUE SPECIFICITY.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis." Razmara M., Srinivasula S.M., Wang L., Poyet J.-L., Geddes B.J., DiStefano P.S., Bertin J., Alnemri E.S. J. Biol. Chem. 277:13952-13958(2002) [PubMed: 11821383] [Abstract] Cited for: INTERACTION WITH CARD8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF208005 mRNA. Translation: AAG23528.1.
AY358231 mRNA. Translation: AAQ88598.1.
BC131610 mRNA. Translation: AAI31611.1.

IPI

IPI00027987.

RefSeq

NP_067546.1.

UniGene

Hs.56279

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DGN	NMR	-	A	2-90	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P57730.

PTM databases

PhosphoSite

P57730.

Proteomic databases

PRIDE

P57730.

Genome annotation databases

Ensembl

ENST00000236886; ENSP00000236886; ENSG00000118168; Homo sapiens. [Genome view]

GeneID

59082.

KEGG

hsa:59082.

UCSC

uc001pis.1. human.

Organism-specific databases

CTD

59082.

GeneCards

GC11M104514.

H-InvDB

HIX0035881.

HGNC

HGNC:28861. CARD18.

MIM

605354. gene.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21444.

HOVERGEN

HBG006113.

InParanoid

P57730.

Gene expression databases

ArrayExpress

P57730.

Bgee

P57730.

CleanEx

HS_CARD18.

Genevestigator

P57730.

GermOnline

ENSG00000118168. Homo sapiens.

Family and domain databases

InterPro

IPR001315. CARD.
IPR011029. DEATH-like.
IPR002398. Pept_C14_p45.
[Graphical view]

Gene3D

G3DSA:1.10.533.10. DEATH_like. 1 hit.

PANTHER

PTHR10454. Pept_C14_p45. 1 hit.

Pfam

PF00619. CARD. 1 hit.
[Graphical view]

SMART

SM00114. CARD. 1 hit.
[Graphical view]

SUPFAM

SSF47986. DEATH_like. 1 hit.

PROSITE

PS50209. CARD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

65139.

SOURCE

Search...

Entry information

Entry name

CAR18_HUMAN

Accession

Primary (citable) accession number: P57730
Secondary accession number(s): A2RRF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	March 2, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.