P57729 (RAB38_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-38

Alternative name(s):
Melanoma antigen NY-MEL-1

Gene names

Name:

RAB38

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in melanosomal transport and docking. Involved in the proper sorting of TYRP1. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Ref.9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Melanosome. Cytoplasmic vesicle › phagosome. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Recruited to phagosomes containing S.aureus or M.tuberculosis. Ref.5 Ref.6 Ref.9
Tissue specificity	Expressed in melanocytes.
Post-translational modification	Although at least one in vitro system can process and methylate the prenylated C-terminal, in an in vitro system that normally express Rab-38 and in vivo the prenylated C-terminal is not proteolytically processed and not methylated.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Palmitate Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phagosome acidification Inferred from mutant phenotype Ref.9. Source: UniProtKB protein transport Non-traceable author statement Ref.1. Source: UniProtKB small GTPase mediated signal transduction Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic vesicle Inferred from direct assay Ref.9. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Non-traceable author statement Ref.1. Source: UniProtKB GTPase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Ras-related protein Rab-38		PRO_0000121251
Regions
	Nucleotide binding	16 – 23	8	GTP By similarity
	Nucleotide binding	65 – 69	5	GTP By similarity
	Nucleotide binding	127 – 130	4	GTP By similarity
	Motif	38 – 46	9	Effector region By similarity
Sites
	Site	208	1	Not methylated
Amino acid modifications
	Lipidation	205	1	S-palmitoyl cysteine Potential
	Lipidation	208	1	S-geranylgeranyl cysteine Probable
Natural variations
	Natural variant	111	1	K → T in a colorectal cancer sample; somatic mutation. Ref.10		VAR_036415

Sequences

	Sequence		Length	Mass (Da)
	P57729 [UniParc]. Last modified January 11, 2001. Version 1. Checksum: 0D16B42A1B237539	FASTA	211	23,712
	10 20 30 40 50 60 MQAPHKEHLY KLLVIGDLGV GKTSIIKRYV HQNFSSHYRA TIGVDFALKV LHWDPETVVR 70 80 90 100 110 120 LQLWDIAGQE RFGNMTRVYY REAMGAFIVF DVTRPATFEA VAKWKNDLDS KLSLPNGKPV 130 140 150 160 170 180 SVVLLANKCD QGKDVLMNNG LKMDQFCKEH GFVGWFETSA KENINIDEAS RCLVKHILAN 190 200 210 ECDLMESIEP DVVKPHLTST KVASCSGCAK S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Serological cloning of a melanocyte rab guanosine 5'-triphosphate-binding protein and a chromosome condensation protein from a melanoma complementary DNA library." Jaeger D., Stockert E., Jaeger E., Guere A.O., Scanlan M.J., Knuth A., Old L.J., Chen Y.-T. Cancer Res. 60:3584-3591(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	SeattleSNPs variation discovery resource Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[5]	"Proteomic analysis of early melanosomes: identification of novel melanosomal proteins." Basrur V., Yang F., Kushimoto T., Higashimoto Y., Yasumoto K., Valencia J., Muller J., Vieira W.D., Watabe H., Shabanowitz J., Hearing V.J., Hunt D.F., Appella E. J. Proteome Res. 2:69-79(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Melanoma.
[6]	"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Melanoma.
[7]	"Rab GTPases containing a CAAX motif are processed post-geranylgeranylation by proteolysis and methylation." Leung K.F., Baron R., Ali B.R., Magee A.I., Seabra M.C. J. Biol. Chem. 282:1487-1497(2007) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-208.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes." Seto S., Tsujimura K., Koide Y. Traffic 12:407-420(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-111.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF235022 mRNA. Translation: AAG30731.1. BT009842 mRNA. Translation: AAP88844.1. DQ178619 Genomic DNA. Translation: ABA03166.1. BC015808 mRNA. Translation: AAH15808.1.
IPI	IPI00939888.
RefSeq	NP_071732.1. NM_022337.2.
UniGene	Hs.591975.
3D structure databases
ProteinModelPortal	P57729.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000243662.
PTM databases
PhosphoSite	P57729.
Polymorphism databases
DMDM	12230516.
Proteomic databases
PaxDb	P57729.
PRIDE	P57729.
Protocols and materials databases
DNASU	23682.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000243662; ENSP00000243662; ENSG00000123892.
GeneID	23682.
KEGG	hsa:23682.
UCSC	uc001pcj.2. human.
Organism-specific databases
CTD	23682.
GeneCards	GC11M087846.
HGNC	HGNC:9776. RAB38.
MIM	606281. gene.
neXtProt	NX_P57729.
PharmGKB	PA34129.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	P57729.
KO	K07923.
OMA	TKWKNDL.
OrthoDB	EOG49GKHH.
PhylomeDB	P57729.
Gene expression databases
Bgee	P57729.
CleanEx	HS_RAB38.
Genevestigator	P57729.
GermOnline	ENSG00000123892. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	23682.
NextBio	46557.
SOURCE	Search...

Entry information

Entry name

RAB38_HUMAN

Accession

Primary (citable) accession number: P57729
Secondary accession number(s): Q53XK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.