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Protein

Poly(rC)-binding protein 3

Gene

Pcbp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.By similarity

GO - Molecular functioni

  • C-rich single-stranded DNA binding Source: MGI
  • double-stranded DNA binding Source: MGI
  • poly(A) RNA binding Source: MGI
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(rC)-binding protein 3
Alternative name(s):
Alpha-CP3
Gene namesi
Name:Pcbp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1890470. Pcbp3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371Poly(rC)-binding protein 3PRO_0000050093Add
BLAST

Proteomic databases

MaxQBiP57722.
PaxDbiP57722.
PeptideAtlasiP57722.
PRIDEiP57722.

PTM databases

iPTMnetiP57722.
PhosphoSiteiP57722.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in testis and fat tissues and lowest in heart.1 Publication

Gene expression databases

BgeeiENSMUSG00000001120.
CleanExiMM_PCBP3.
ExpressionAtlasiP57722. baseline and differential.
GenevisibleiP57722. MM.

Interactioni

Protein-protein interaction databases

BioGridi208528. 1 interaction.
IntActiP57722. 1 interaction.
MINTiMINT-4106666.
STRINGi10090.ENSMUSP00000001148.

Structurei

3D structure databases

ProteinModelPortaliP57722.
SMRiP57722. Positions 43-231, 258-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 9551KH 1PROSITE-ProRule annotationAdd
BLAST
Domaini129 – 18254KH 2PROSITE-ProRule annotationAdd
BLAST
Domaini293 – 35765KH 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2190. Eukaryota.
ENOG410XNN8. LUCA.
GeneTreeiENSGT00760000119144.
HOGENOMiHOG000182823.
HOVERGENiHBG053520.
InParanoidiP57722.
KOiK13162.
OMAiEDSHNAD.
OrthoDBiEOG091G0DHI.
PhylomeDBiP57722.
TreeFamiTF318292.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P57722-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEGDAIWAP PILPHSTLGT LSHHPELHFG GKMESKVSEG GLNVTLTIRL
60 70 80 90 100
LMHGKEVGSI IGKKGETVKK MREESGARIN ISEGNCPERI VTITGPTDAI
110 120 130 140 150
FKAFAMIAYK FEEDIINSMS NSPATSKPPV TLRLVVPASQ CGSLIGKGGS
160 170 180 190 200
KIKEIRESTG AQVQVAGDML PNSTERAVTI SGTPDAIIQC VKQICVVMLE
210 220 230 240 250
SPPKGATIPY RPKPASTPVI FAGGQAYTIQ GQYAIPHPDQ LTKLHQLAMQ
260 270 280 290 300
QTPFPPLGQT NPAFPGEKLP LHSSEEAQNL MGQSSGLDAS PPASTHELTI
310 320 330 340 350
PNDLIGCIIG RQGTKINEIR QMSGAQIKIA NATEGSSERQ ITITGTPANI
360 370
SLAQYLINAR LTSEVTGMGA L
Length:371
Mass (Da):39,294
Last modified:May 18, 2010 - v3
Checksum:iBDBAFEB7EA99DDD7
GO
Isoform 2 (identifier: P57722-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     240-240: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:370
Mass (Da):39,166
Checksum:i9E0E2900460FE7FA
GO

Sequence cautioni

The sequence AAG09238 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881I → F in AAG09238 (PubMed:10936052).Curated
Sequence conflicti216 – 2161S → C in AAG09238 (PubMed:10936052).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei240 – 2401Missing in isoform 2. 1 PublicationVSP_010015

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176327 mRNA. Translation: AAG09238.1. Different initiation.
AK034811 mRNA. Translation: BAC28838.1.
AK079564 mRNA. Translation: BAC37686.1.
CCDSiCCDS35946.2. [P57722-1]
RefSeqiNP_067543.2. NM_021568.2. [P57722-1]
XP_006513986.1. XM_006513923.2. [P57722-2]
UniGeneiMm.272803.

Genome annotation databases

EnsembliENSMUST00000001148; ENSMUSP00000001148; ENSMUSG00000001120. [P57722-1]
ENSMUST00000105411; ENSMUSP00000101051; ENSMUSG00000001120. [P57722-2]
ENSMUST00000168465; ENSMUSP00000129465; ENSMUSG00000001120. [P57722-1]
GeneIDi59093.
KEGGimmu:59093.
UCSCiuc007fuz.2. mouse. [P57722-1]
uc007fva.2. mouse. [P57722-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176327 mRNA. Translation: AAG09238.1. Different initiation.
AK034811 mRNA. Translation: BAC28838.1.
AK079564 mRNA. Translation: BAC37686.1.
CCDSiCCDS35946.2. [P57722-1]
RefSeqiNP_067543.2. NM_021568.2. [P57722-1]
XP_006513986.1. XM_006513923.2. [P57722-2]
UniGeneiMm.272803.

3D structure databases

ProteinModelPortaliP57722.
SMRiP57722. Positions 43-231, 258-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208528. 1 interaction.
IntActiP57722. 1 interaction.
MINTiMINT-4106666.
STRINGi10090.ENSMUSP00000001148.

PTM databases

iPTMnetiP57722.
PhosphoSiteiP57722.

Proteomic databases

MaxQBiP57722.
PaxDbiP57722.
PeptideAtlasiP57722.
PRIDEiP57722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001148; ENSMUSP00000001148; ENSMUSG00000001120. [P57722-1]
ENSMUST00000105411; ENSMUSP00000101051; ENSMUSG00000001120. [P57722-2]
ENSMUST00000168465; ENSMUSP00000129465; ENSMUSG00000001120. [P57722-1]
GeneIDi59093.
KEGGimmu:59093.
UCSCiuc007fuz.2. mouse. [P57722-1]
uc007fva.2. mouse. [P57722-2]

Organism-specific databases

CTDi54039.
MGIiMGI:1890470. Pcbp3.

Phylogenomic databases

eggNOGiKOG2190. Eukaryota.
ENOG410XNN8. LUCA.
GeneTreeiENSGT00760000119144.
HOGENOMiHOG000182823.
HOVERGENiHBG053520.
InParanoidiP57722.
KOiK13162.
OMAiEDSHNAD.
OrthoDBiEOG091G0DHI.
PhylomeDBiP57722.
TreeFamiTF318292.

Miscellaneous databases

ChiTaRSiPcbp3. mouse.
PROiP57722.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001120.
CleanExiMM_PCBP3.
ExpressionAtlasiP57722. baseline and differential.
GenevisibleiP57722. MM.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCBP3_MOUSE
AccessioniPrimary (citable) accession number: P57722
Secondary accession number(s): Q8BSB0, Q8C544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 18, 2010
Last modified: September 7, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.