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Protein

Chorismate synthase, chloroplastic

Gene

EMB1144

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the last common step of the biosynthesis of aromatic amino acids, produced via the shikimic acid pathway.By similarity

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.

Cofactori

FMNH2By similarity

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic (DHS2), Phospho-2-dehydro-3-deoxyheptonate aldolase (At4g33510), Phospho-2-dehydro-3-deoxyheptonate aldolase (At4g33510), Phospho-2-dehydro-3-deoxyheptonate aldolase (At1g22410), Phospho-2-dehydro-3-deoxyheptonate aldolase (AXX17_At1g23540), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phospho-2-dehydro-3-deoxyheptonate aldolase (DHS2), Phospho-2-dehydro-3-deoxyheptonate aldolase (AXX17_At4g38330), Phospho-2-dehydro-3-deoxyheptonate aldolase (AXX17_At4g45420), Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic (DHS1)
  2. 3-dehydroquinate synthase, chloroplastic (DHQS), Uncharacterized protein (AXX17_At5g66050)
  3. Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (EMB3004)
  4. Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (EMB3004)
  5. Shikimate kinase 2, chloroplastic (SK2), Shikimate kinase 1, chloroplastic (SK1)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (F27K7.11), 3-phosphoshikimate 1-carboxyvinyltransferase (At2g45300), 3-phosphoshikimate 1-carboxyvinyltransferase, 3-phosphoshikimate 1-carboxyvinyltransferase (AXX17_At2g42890), 3-phosphoshikimate 1-carboxyvinyltransferase (AXX17_At1g42980), 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic (At2g45300)
  7. Chorismate synthase (At1g48850), Chorismate synthase, chloroplastic (EMB1144), Chorismate synthase (AXX17_At1g42970), Chorismate synthase (EMB1144), Chorismate synthase (EMB1144)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciARA:AT1G48850-MONOMER.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthase, chloroplastic (EC:4.2.3.5)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyase
Protein EMBRYO DEFECTIVE 1144
Gene namesi
Name:EMB1144
Ordered Locus Names:At1g48850
ORF Names:F27K7.1, T24P22.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G48850.
TAIRilocus:2028549. AT1G48850.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50ChloroplastCuratedAdd BLAST50
ChainiPRO_000000229451 – 436Chorismate synthase, chloroplasticAdd BLAST386

Proteomic databases

PaxDbiP57720.

PTM databases

iPTMnetiP57720.

Expressioni

Gene expression databases

ExpressionAtlasiP57720. baseline and differential.
GenevisibleiP57720. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G48850.1.

Structurei

3D structure databases

ProteinModelPortaliP57720.
SMRiP57720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4492. Eukaryota.
COG0082. LUCA.
HOGENOMiHOG000060335.
InParanoidiP57720.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG09360BCK.
PhylomeDBiP57720.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiView protein in InterPro
IPR000453. Chorismate_synth.
IPR035904. Chorismate_synth_AroC_sf.
IPR020541. Chorismate_synthase_CS.
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiView protein in Pfam
PF01264. Chorismate_synt. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiView protein in PROSITE
PS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P57720-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSLTSKS ILGSTKLGSS SLPSELRRLS SPAVQISLRT QTRKNFQIQA
60 70 80 90 100
TGSSYGTHFR VSTFGESHGG GVGCIIDGCP PRIPLTESDL QFDLDRRRPG
110 120 130 140 150
QSRITTPRKE TDTCRISSGV SEGMTTGTPI HVFVPNTDQR GLDYSEMSVA
160 170 180 190 200
YRPSHADATY DMKYGVRSVQ GGGRSSARET IGRVAPGALA KKILKQFAGT
210 220 230 240 250
EILAYVSQVH HVVLPEELVD HENLTLEQIE NNIVRCPNPE YAEKMIAAID
260 270 280 290 300
AVRTKGNSVG GVVTCIVRNA PRGLGTPVFD KLEAELAKAC MSLPATKGFE
310 320 330 340 350
FGSGFAGTFL TGLEHNDEFY TDENGRIRTR TNRSGGIQGG ISNGEIINMR
360 370 380 390 400
VAFKPTSTIG RKQNTVTRDK VETEMIARGR HDPCVVPRAV PMVEAMVALV
410 420 430
LVDQLMAQYA QCHLFPINPE LQEPLQIEQP QNATAL
Length:436
Mass (Da):47,318
Last modified:December 6, 2005 - v2
Checksum:i6753188EA056680F
GO

Sequence cautioni

The sequence AAG29740 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG50662 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD93818 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC084242 Genomic DNA. Translation: AAG50662.1. Sequence problems.
AC084414 Genomic DNA. Translation: AAG29740.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32356.1.
AY057519 mRNA. Translation: AAL09759.1.
BT015785 mRNA. Translation: AAU90075.1.
AK220709 mRNA. Translation: BAD93818.1. Different initiation.
PIRiC96526.
RefSeqiNP_564534.1. NM_103779.5. [P57720-1]
UniGeneiAt.43673.
At.48292.

Genome annotation databases

EnsemblPlantsiAT1G48850.1; AT1G48850.1; AT1G48850. [P57720-1]
GeneIDi841307.
GrameneiAT1G48850.1; AT1G48850.1; AT1G48850.
KEGGiath:AT1G48850.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAROC_ARATH
AccessioniPrimary (citable) accession number: P57720
Secondary accession number(s): Q570J9, Q93ZI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: December 6, 2005
Last modified: November 22, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families