Reviewed, UniProtKB/Swiss-Prot P57720 (AROC_ARATH)

Names and origin

Protein names

Recommended name:
    Chorismate synthase, chloroplastic
    EC=4.2.3.5
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate phospholyase
    Protein EMBRYO DEFECTIVE 1144

Gene names

Name:	EMB1144
Ordered Locus Names:	At1g48850
ORF Names:	T24P22.3, F27K7.1

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the last common step of the biosynthesis of aromatic amino acids, produced via the shikimic acid pathway By similarity.
Catalytic activity	5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.
Cofactor	Reduced flavin By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the chorismate synthase family.
Sequence caution	The sequence AAG29740.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAG50662.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from direct assay. Source: TAIR nucleolus Inferred from direct assay. Source: TAIR
Molecular function	chorismate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P57720-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 436	387	Chorismate synthase, chloroplastic		PRO_0000002294

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 6753188EA056680F	FASTA	436	47,318
	10 20 30 40 50 60 MASSSLTSKS ILGSTKLGSS SLPSELRRLS SPAVQISLRT QTRKNFQIQA TGSSYGTHFR 70 80 90 100 110 120 VSTFGESHGG GVGCIIDGCP PRIPLTESDL QFDLDRRRPG QSRITTPRKE TDTCRISSGV 130 140 150 160 170 180 SEGMTTGTPI HVFVPNTDQR GLDYSEMSVA YRPSHADATY DMKYGVRSVQ GGGRSSARET 190 200 210 220 230 240 IGRVAPGALA KKILKQFAGT EILAYVSQVH HVVLPEELVD HENLTLEQIE NNIVRCPNPE 250 260 270 280 290 300 YAEKMIAAID AVRTKGNSVG GVVTCIVRNA PRGLGTPVFD KLEAELAKAC MSLPATKGFE 310 320 330 340 350 360 FGSGFAGTFL TGLEHNDEFY TDENGRIRTR TNRSGGIQGG ISNGEIINMR VAFKPTSTIG 370 380 390 400 410 420 RKQNTVTRDK VETEMIARGR HDPCVVPRAV PMVEAMVALV LVDQLMAQYA QCHLFPINPE 430 LQEPLQIEQP QNATAL

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 335-436. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC084242 Genomic DNA. Translation: AAG50662.1. Sequence problems. AC084414 Genomic DNA. Translation: AAG29740.1. Sequence problems. AY057519 mRNA. Translation: AAL09759.1. BT015785 mRNA. Translation: AAU90075.1. AK220709 mRNA. Translation: BAD93818.1. Different initiation.
IPI	IPI00534108.
PIR	C96526.
RefSeq	NP_564534.1.
UniGene	At.48292
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P57720.
Proteomic databases
PRIDE	P57720.
Genome annotation databases
GeneID	841307.
GenomeReviews	Gene locus AT1G48850 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g48850.
Phylogenomic databases
OMA	QASIERS.
Enzyme and pathway databases
BRENDA	4.2.3.5. 302.
Gene expression databases
ArrayExpress	P57720.
Genevestigator	P57720.
Family and domain databases
InterPro	IPR000453. Chorismate_synth. IPR020541. Chorismate_synthase_CS. [Graphical view]
PANTHER	PTHR21085. Chorismate_synth. 1 hit.
Pfam	PF01264. Chorismate_synt. 1 hit. [Graphical view]
PIRSF	PIRSF001456. Chorismate_synth. 1 hit.
ProDom	PD002941. Chorismate_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00033. aroC. 1 hit.
PROSITE	PS00787. CHORISMATE_SYNTHASE_1. 1 hit. PS00788. CHORISMATE_SYNTHASE_2. 1 hit. PS00789. CHORISMATE_SYNTHASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROC_ARATH

Accession

Primary (citable) accession number: P57720
Secondary accession number(s): Q570J9, Q93ZI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)