Nicastrin precursor - Mus musculus (Mouse)

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P57716 (NICA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nicastrin

Gene names

Name:

Ncstn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
Subunit structure	Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP) By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity. Melanosome By similarity.
Sequence similarities	Belongs to the nicastrin family.

Ontologies

Keywords
Biological process	Notch signaling pathway
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW T cell proliferation Inferred from mutant phenotype PubMed 17913918. Source: MGI beta-amyloid metabolic process Inferred from mutant phenotype PubMed 17913918. Source: MGI epithelial cell proliferation Inferred from mutant phenotype PubMed 17913918. Source: MGI membrane protein ectodomain proteolysis Inferred from sequence or structural similarity. Source: UniProtKB myeloid cell homeostasis Inferred from mutant phenotype PubMed 17913918. Source: MGI positive regulation of catalytic activity Inferred from sequence or structural similarity. Source: UniProtKB protein processing Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB integral to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB lysosomal membrane Inferred from electronic annotation. Source: Compara melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	endopeptidase activity Inferred from mutant phenotype PubMed 17913918. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

Potential

Chain

28 – 708

681

Nicastrin

PRO_0000019682

Regions

Topological domain

28 – 668

641

Extracellular Potential

Transmembrane

669 – 689

Helical; Potential

Topological domain

690 – 708

Cytoplasmic Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

128

N-linked (GlcNAc...) Potential

Glycosylation

186

N-linked (GlcNAc...) Ref.4

Glycosylation

203

N-linked (GlcNAc...) Potential

Glycosylation

263

N-linked (GlcNAc...) Ref.4

Glycosylation

386

N-linked (GlcNAc...) Ref.4

Glycosylation

434

N-linked (GlcNAc...) Potential

Glycosylation

463

N-linked (GlcNAc...) Potential

Glycosylation

505

N-linked (GlcNAc...) Ref.4

Glycosylation

529

N-linked (GlcNAc...) Potential

Glycosylation

530

N-linked (GlcNAc...) Ref.4

Glycosylation

561

N-linked (GlcNAc...) Ref.4

Glycosylation

572

N-linked (GlcNAc...) Potential

Glycosylation

579

N-linked (GlcNAc...) Potential

Glycosylation

593

N-linked (GlcNAc...) Potential

Glycosylation

611

N-linked (GlcNAc...) Ref.4

Experimental info

Sequence conflict

527

R → K in AAG11413. Ref.1

Sequence conflict

527

R → K in AAH19998. Ref.3

Sequence conflict

666

E → K in AAG11413. Ref.1

Sequence conflict

678 – 680

TLV → ILI in AAG11413. Ref.1

Sequence conflict

678 – 680

TLV → ILI in AAH19998. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P57716 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 32B57BB478FEB0AC
Show »

FASTA

708

78,492

        10         20         30         40         50         60 
MATTRGGSGP DPGSRGLLLL SFSVVLAGLC GGNSVERKIY IPLNKTAPCV RLLNATHQIG 

        70         80         90        100        110        120 
CQSSISGDTG VIHVVEKEED LKWVLTDGPN PPYMVLLEGK LFTRDVMEKL KGTTSRIAGL 

       130        140        150        160        170        180 
AVTLAKPNST SSFSPSVQCP NDGFGIYSNS YGPEFAHCKK TLWNELGNGL AYEDFSFPIF 

       190        200        210        220        230        240 
LLEDENETKV IKQCYQDHNL GQNGSAPSFP LCAMQLFSHM HAVISTATCM RRSFIQSTFS 

       250        260        270        280        290        300 
INPEIVCDPL SDYNVWSMLK PINTSVGLEP DVRVVVAATR LDSRSFFWNV APGAESAVAS 

       310        320        330        340        350        360 
FVTQLAAAEA LHKAPDVTTL SRNVMFVFFQ GETFDYIGSS RMVYDMENGK FPVRLENIDS 

       370        380        390        400        410        420 
FVELGQVALR TSLDLWMHTD PMSQKNESVK NQVEDLLATL EKSGAGVPEV VLRRLAQSQA 

       430        440        450        460        470        480 
LPPSSLQRFL RARNISGVVL ADHSGSFHNR YYQSIYDTAE NINVTYPEWQ SPEEDLNFVT 

       490        500        510        520        530        540 
DTAKALANVA TVLARALYEL AGGTNFSSSI QADPQTVTRL LYGFLVRANN SWFQSILKHD 

       550        560        570        580        590        600 
LRSYLDDRPL QHYIAVSSPT NTTYVVQYAL ANLTGKATNL TREQCQDPSK VPNESKDLYE 

       610        620        630        640        650        660 
YSWVQGPWNS NRTERLPQCV RSTVRLARAL SPAFELSQWS STEYSTWAES RWKDIQARIF 

       670        680        690        700 
LIASKELEFI TLIVGFSTLV FSLIVTYCIN AKADVLFVAP REPGAVSY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing." Yu G., Nishimura M., Arawaka S., Levitan D., Zhang L., Tandon A., Song Y.-Q., Rogaeva E., Chen F., Kawarai T., Supala A., Levesque L., Yu H., Yang D.-S., Holmes E., Milman P., Liang Y., Zhang D.M. St George-Hyslop P.H. Nature 407:48-54(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-186; ASN-263; ASN-386; ASN-505; ASN-530; ASN-561 AND ASN-611, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF240469 mRNA. Translation: AAG11413.1. AC158930 Genomic DNA. No translation available. BC019998 mRNA. Translation: AAH19998.1.
IPI	IPI00118674.
RefSeq	NP_067620.3. NM_021607.3.
UniGene	Mm.218203.
3D structure databases
ProteinModelPortal	P57716.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-36334N.
IntAct	P57716. 4 interactions.
MINT	MINT-1177052.
PTM databases
PhosphoSite	P57716.
Proteomic databases
PaxDb	P57716.
PRIDE	P57716.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003550; ENSMUSP00000003550; ENSMUSG00000003458.
GeneID	59287.
KEGG	mmu:59287.
Organism-specific databases
CTD	23385.
MGI	MGI:1891700. Ncstn.
Phylogenomic databases
eggNOG	NOG253370.
GeneTree	ENSGT00390000014633.
HOVERGEN	HBG006497.
InParanoid	P57716.
KO	K06171.
OMA	HMHAVIS.
OrthoDB	EOG4STS48.
Enzyme and pathway databases
Reactome	REACT_115202. Signal Transduction. REACT_99130. A third proteolytic cleavage releases NICD.
Gene expression databases
ArrayExpress	P57716.
Bgee	P57716.
CleanEx	MM_NCSTN.
Genevestigator	P57716.
GermOnline	ENSMUSG00000003458. Mus musculus.
Family and domain databases
InterPro	IPR008710. Nicastrin. [Graphical view]
PANTHER	PTHR21092. PTHR21092. 1 hit.
Pfam	PF05450. Nicastrin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P57716.
ChEMBL	CHEMBL4548.
NextBio	314784.
SOURCE	Search...

Entry information

Entry name

NICA_MOUSE

Accession

Primary (citable) accession number: P57716
Secondary accession number(s): E9QLZ6, Q8VE20

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents