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P57682 (KLF3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Krueppel-like factor 3
Alternative name(s):
Basic krueppel-like factor
CACCC-box-binding protein BKLF
TEF-2
Gene names
Name:KLF3
Synonyms:BKLF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Nucleus Probable.

Post-translational modification

Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site By similarity.

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Contains 3 C2H2-type zinc fingers.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P57682-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P57682-2)

The sequence of this isoform differs from the canonical sequence as follows:
     233-345: Missing.
Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Krueppel-like factor 3
PRO_0000047165

Regions

Zinc finger260 – 28425C2H2-type 1
Zinc finger290 – 31425C2H2-type 2
Zinc finger320 – 34223C2H2-type 3
Region1 – 7474Repressor domain
Motif61 – 655CTBP-binding motif
Compositional bias1 – 251251Pro-rich

Amino acid modifications

Modified residue921Phosphoserine Ref.5 Ref.6
Modified residue1011Phosphoserine Ref.5
Modified residue1111Phosphoserine Ref.6
Modified residue2501Phosphoserine Ref.4
Cross-link10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link198Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Natural variations

Alternative sequence233 – 345113Missing in isoform 2.
VSP_014532
Natural variant2071R → S.
Corresponds to variant rs17616226 [ dbSNP | Ensembl ].
VAR_052715

Experimental info

Sequence conflict851P → R in AAH51687. Ref.3
Sequence conflict1161P → H in AAH51687. Ref.3
Sequence conflict3051D → G in AAH30662. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: A490D642AB8F1FDC

FASTA34538,829
        10         20         30         40         50         60 
MLMFDPVPVK QEAMDPVSVS YPSNYMESMK PNKYGVIYST PLPEKFFQTP EGLSHGIQME 

        70         80         90        100        110        120 
PVDLTVNKRS SPPSAGNSPS SLKFPSSHRR ASPGLSMPSS SPPIKKYSPP SPGVQPFGVP 

       130        140        150        160        170        180 
LSMPPVMAAA LSRHGIRSPG ILPVIQPVVV QPVPFMYTSH LQQPLMVSLS EEMENSSSSM 

       190        200        210        220        230        240 
QVPVIESYEK PISQKKIKIE PGIEPQRTDY YPEEMSPPLM NSVSPPQALL QENHPSVIVQ 

       250        260        270        280        290        300 
PGKRPLPVES PDTQRKRRIH RCDYDGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK 

       310        320        330        340 
FARSDELTRH FRKHTGIKPF QCPDCDRSFS RSDHLALHRK RHMLV 

« Hide

Isoform 2 [UniParc].

Checksum: 20A838F91F92DD50
Show »

FASTA23225,430

References

« Hide 'large scale' references
[1]Matsumoto N., Yoshida T., Terada M.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"cDNA cloning, subcellular localization and tissue expression of a new human Kruppel-like transcription factor: human basic Kruppel-like factor (hBKLF)."
Wang M.J., Qu X.H., Wang L.S., Zhai Y., Wu S.L., He F.C.
Yi Chuan Xue Bao 30:1-9(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Hippocampus.
[4]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB024523 mRNA. Translation: BAA92271.1.
AF285837 mRNA. Translation: AAK27329.1.
BC030662 mRNA. Translation: AAH30662.1.
BC051687 mRNA. Translation: AAH51687.1.
CCDSCCDS3444.1. [P57682-1]
RefSeqNP_057615.3. NM_016531.5. [P57682-1]
UniGeneHs.298658.

3D structure databases

ProteinModelPortalP57682.
SMRP57682. Positions 251-345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119426. 5 interactions.
IntActP57682. 3 interactions.
MINTMINT-4786517.
STRING9606.ENSP00000261438.

PTM databases

PhosphoSiteP57682.

Polymorphism databases

DMDM12644533.

Proteomic databases

MaxQBP57682.
PaxDbP57682.
PRIDEP57682.

Protocols and materials databases

DNASU51274.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261438; ENSP00000261438; ENSG00000109787. [P57682-1]
ENST00000514033; ENSP00000421252; ENSG00000109787. [P57682-2]
GeneID51274.
KEGGhsa:51274.
UCSCuc003gtg.2. human. [P57682-1]

Organism-specific databases

CTD51274.
GeneCardsGC04P038668.
HGNCHGNC:16516. KLF3.
HPAHPA049512.
MIM609392. gene.
neXtProtNX_P57682.
PharmGKBPA30137.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5048.
HOGENOMHOG000232138.
HOVERGENHBG003941.
InParanoidP57682.
KOK15605.
OMANSSMQVP.
OrthoDBEOG747PJ4.
PhylomeDBP57682.
TreeFamTF350556.

Gene expression databases

BgeeP57682.
CleanExHS_KLF3.
GenevestigatorP57682.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKLF3.
GenomeRNAi51274.
NextBio54481.
PROP57682.
SOURCESearch...

Entry information

Entry nameKLF3_HUMAN
AccessionPrimary (citable) accession number: P57682
Secondary accession number(s): Q6PIR1, Q86TN0, Q9P2X6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM