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Protein

Krueppel-like factor 3

Gene

KLF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri260 – 28425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri290 – 31425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri320 – 34223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  • cellular response to peptide Source: Ensembl
  • multicellular organismal development Source: ProtInc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 3
Alternative name(s):
Basic krueppel-like factor
CACCC-box-binding protein BKLF
TEF-2
Gene namesi
Name:KLF3
Synonyms:BKLF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:16516. KLF3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30137.

Polymorphism and mutation databases

BioMutaiKLF3.
DMDMi12644533.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Krueppel-like factor 3PRO_0000047165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki10 – 10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei92 – 921Phosphoserine2 Publications
Modified residuei101 – 1011Phosphoserine1 Publication
Modified residuei111 – 1111Phosphoserine1 Publication
Cross-linki198 – 198Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei250 – 2501Phosphoserine1 Publication

Post-translational modificationi

Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP57682.
PaxDbiP57682.
PRIDEiP57682.

PTM databases

PhosphoSiteiP57682.

Expressioni

Gene expression databases

BgeeiP57682.
CleanExiHS_KLF3.
ExpressionAtlasiP57682. baseline and differential.
GenevisibleiP57682. HS.

Organism-specific databases

HPAiHPA049512.

Interactioni

Subunit structurei

Monomer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
DVL3Q929973EBI-8472267,EBI-739789
EHMT2A2ABF93EBI-8472267,EBI-10174566
FHL3Q136434EBI-8472267,EBI-741101
LHX8Q68G743EBI-8472267,EBI-8474075
TRAF2Q129333EBI-8472267,EBI-355744

Protein-protein interaction databases

BioGridi119426. 9 interactions.
IntActiP57682. 8 interactions.
MINTiMINT-4786517.
STRINGi9606.ENSP00000261438.

Structurei

3D structure databases

ProteinModelPortaliP57682.
SMRiP57682. Positions 251-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7474Repressor domainAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 655CTBP-binding motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 251251Pro-richAdd
BLAST

Sequence similaritiesi

Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri260 – 28425C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri290 – 31425C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri320 – 34223C2H2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000232138.
HOVERGENiHBG003941.
InParanoidiP57682.
KOiK15605.
OMAiLPDKFFQ.
OrthoDBiEOG747PJ4.
PhylomeDBiP57682.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P57682-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLMFDPVPVK QEAMDPVSVS YPSNYMESMK PNKYGVIYST PLPEKFFQTP
60 70 80 90 100
EGLSHGIQME PVDLTVNKRS SPPSAGNSPS SLKFPSSHRR ASPGLSMPSS
110 120 130 140 150
SPPIKKYSPP SPGVQPFGVP LSMPPVMAAA LSRHGIRSPG ILPVIQPVVV
160 170 180 190 200
QPVPFMYTSH LQQPLMVSLS EEMENSSSSM QVPVIESYEK PISQKKIKIE
210 220 230 240 250
PGIEPQRTDY YPEEMSPPLM NSVSPPQALL QENHPSVIVQ PGKRPLPVES
260 270 280 290 300
PDTQRKRRIH RCDYDGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK
310 320 330 340
FARSDELTRH FRKHTGIKPF QCPDCDRSFS RSDHLALHRK RHMLV
Length:345
Mass (Da):38,829
Last modified:December 1, 2000 - v1
Checksum:iA490D642AB8F1FDC
GO
Isoform 2 (identifier: P57682-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-345: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:232
Mass (Da):25,430
Checksum:i20A838F91F92DD50
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851P → R in AAH51687 (PubMed:15489334).Curated
Sequence conflicti116 – 1161P → H in AAH51687 (PubMed:15489334).Curated
Sequence conflicti305 – 3051D → G in AAH30662 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti207 – 2071R → S.
Corresponds to variant rs17616226 [ dbSNP | Ensembl ].
VAR_052715

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei233 – 345113Missing in isoform 2. 1 PublicationVSP_014532Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024523 mRNA. Translation: BAA92271.1.
AF285837 mRNA. Translation: AAK27329.1.
BC030662 mRNA. Translation: AAH30662.1.
BC051687 mRNA. Translation: AAH51687.1.
CCDSiCCDS3444.1. [P57682-1]
RefSeqiNP_057615.3. NM_016531.5. [P57682-1]
UniGeneiHs.298658.

Genome annotation databases

EnsembliENST00000261438; ENSP00000261438; ENSG00000109787.
ENST00000514033; ENSP00000421252; ENSG00000109787. [P57682-2]
GeneIDi51274.
KEGGihsa:51274.
UCSCiuc003gtg.2. human. [P57682-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024523 mRNA. Translation: BAA92271.1.
AF285837 mRNA. Translation: AAK27329.1.
BC030662 mRNA. Translation: AAH30662.1.
BC051687 mRNA. Translation: AAH51687.1.
CCDSiCCDS3444.1. [P57682-1]
RefSeqiNP_057615.3. NM_016531.5. [P57682-1]
UniGeneiHs.298658.

3D structure databases

ProteinModelPortaliP57682.
SMRiP57682. Positions 251-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119426. 9 interactions.
IntActiP57682. 8 interactions.
MINTiMINT-4786517.
STRINGi9606.ENSP00000261438.

PTM databases

PhosphoSiteiP57682.

Polymorphism and mutation databases

BioMutaiKLF3.
DMDMi12644533.

Proteomic databases

MaxQBiP57682.
PaxDbiP57682.
PRIDEiP57682.

Protocols and materials databases

DNASUi51274.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261438; ENSP00000261438; ENSG00000109787.
ENST00000514033; ENSP00000421252; ENSG00000109787. [P57682-2]
GeneIDi51274.
KEGGihsa:51274.
UCSCiuc003gtg.2. human. [P57682-1]

Organism-specific databases

CTDi51274.
GeneCardsiGC04P038668.
HGNCiHGNC:16516. KLF3.
HPAiHPA049512.
MIMi609392. gene.
neXtProtiNX_P57682.
PharmGKBiPA30137.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118998.
HOGENOMiHOG000232138.
HOVERGENiHBG003941.
InParanoidiP57682.
KOiK15605.
OMAiLPDKFFQ.
OrthoDBiEOG747PJ4.
PhylomeDBiP57682.
TreeFamiTF350556.

Miscellaneous databases

ChiTaRSiKLF3. human.
GeneWikiiKLF3.
GenomeRNAii51274.
NextBioi54481.
PROiP57682.
SOURCEiSearch...

Gene expression databases

BgeeiP57682.
CleanExiHS_KLF3.
ExpressionAtlasiP57682. baseline and differential.
GenevisibleiP57682. HS.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Matsumoto N., Yoshida T., Terada M.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "cDNA cloning, subcellular localization and tissue expression of a new human Kruppel-like transcription factor: human basic Kruppel-like factor (hBKLF)."
    Wang M.J., Qu X.H., Wang L.S., Zhai Y., Wu S.L., He F.C.
    Yi Chuan Xue Bao 30:1-9(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Hippocampus.
  4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiKLF3_HUMAN
AccessioniPrimary (citable) accession number: P57682
Secondary accession number(s): Q6PIR1, Q86TN0, Q9P2X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 22, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.