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P57682

- KLF3_HUMAN

UniProt

P57682 - KLF3_HUMAN

Protein

Krueppel-like factor 3

Gene

KLF3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri260 – 28425C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri290 – 31425C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri320 – 34223C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. cellular response to peptide Source: Ensembl
    2. multicellular organismal development Source: ProtInc
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Krueppel-like factor 3
    Alternative name(s):
    Basic krueppel-like factor
    CACCC-box-binding protein BKLF
    TEF-2
    Gene namesi
    Name:KLF3
    Synonyms:BKLF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:16516. KLF3.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30137.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 345345Krueppel-like factor 3PRO_0000047165Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki10 – 10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei92 – 921Phosphoserine2 Publications
    Modified residuei101 – 1011Phosphoserine1 Publication
    Modified residuei111 – 1111Phosphoserine1 Publication
    Cross-linki198 – 198Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei250 – 2501Phosphoserine1 Publication

    Post-translational modificationi

    Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP57682.
    PaxDbiP57682.
    PRIDEiP57682.

    PTM databases

    PhosphoSiteiP57682.

    Expressioni

    Gene expression databases

    BgeeiP57682.
    CleanExiHS_KLF3.
    GenevestigatoriP57682.

    Organism-specific databases

    HPAiHPA049512.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi119426. 5 interactions.
    IntActiP57682. 3 interactions.
    MINTiMINT-4786517.
    STRINGi9606.ENSP00000261438.

    Structurei

    3D structure databases

    ProteinModelPortaliP57682.
    SMRiP57682. Positions 251-345.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7474Repressor domainAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 655CTBP-binding motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1 – 251251Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri260 – 28425C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri290 – 31425C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri320 – 34223C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000232138.
    HOVERGENiHBG003941.
    InParanoidiP57682.
    KOiK15605.
    OMAiNSSMQVP.
    OrthoDBiEOG747PJ4.
    PhylomeDBiP57682.
    TreeFamiTF350556.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P57682-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLMFDPVPVK QEAMDPVSVS YPSNYMESMK PNKYGVIYST PLPEKFFQTP    50
    EGLSHGIQME PVDLTVNKRS SPPSAGNSPS SLKFPSSHRR ASPGLSMPSS 100
    SPPIKKYSPP SPGVQPFGVP LSMPPVMAAA LSRHGIRSPG ILPVIQPVVV 150
    QPVPFMYTSH LQQPLMVSLS EEMENSSSSM QVPVIESYEK PISQKKIKIE 200
    PGIEPQRTDY YPEEMSPPLM NSVSPPQALL QENHPSVIVQ PGKRPLPVES 250
    PDTQRKRRIH RCDYDGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK 300
    FARSDELTRH FRKHTGIKPF QCPDCDRSFS RSDHLALHRK RHMLV 345
    Length:345
    Mass (Da):38,829
    Last modified:December 1, 2000 - v1
    Checksum:iA490D642AB8F1FDC
    GO
    Isoform 2 (identifier: P57682-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         233-345: Missing.

    Note: May be due to intron retention. No experimental confirmation available.

    Show »
    Length:232
    Mass (Da):25,430
    Checksum:i20A838F91F92DD50
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851P → R in AAH51687. (PubMed:15489334)Curated
    Sequence conflicti116 – 1161P → H in AAH51687. (PubMed:15489334)Curated
    Sequence conflicti305 – 3051D → G in AAH30662. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti207 – 2071R → S.
    Corresponds to variant rs17616226 [ dbSNP | Ensembl ].
    VAR_052715

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei233 – 345113Missing in isoform 2. 1 PublicationVSP_014532Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024523 mRNA. Translation: BAA92271.1.
    AF285837 mRNA. Translation: AAK27329.1.
    BC030662 mRNA. Translation: AAH30662.1.
    BC051687 mRNA. Translation: AAH51687.1.
    CCDSiCCDS3444.1. [P57682-1]
    RefSeqiNP_057615.3. NM_016531.5. [P57682-1]
    UniGeneiHs.298658.

    Genome annotation databases

    EnsembliENST00000261438; ENSP00000261438; ENSG00000109787. [P57682-1]
    ENST00000514033; ENSP00000421252; ENSG00000109787. [P57682-2]
    GeneIDi51274.
    KEGGihsa:51274.
    UCSCiuc003gtg.2. human. [P57682-1]

    Polymorphism databases

    DMDMi12644533.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024523 mRNA. Translation: BAA92271.1 .
    AF285837 mRNA. Translation: AAK27329.1 .
    BC030662 mRNA. Translation: AAH30662.1 .
    BC051687 mRNA. Translation: AAH51687.1 .
    CCDSi CCDS3444.1. [P57682-1 ]
    RefSeqi NP_057615.3. NM_016531.5. [P57682-1 ]
    UniGenei Hs.298658.

    3D structure databases

    ProteinModelPortali P57682.
    SMRi P57682. Positions 251-345.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119426. 5 interactions.
    IntActi P57682. 3 interactions.
    MINTi MINT-4786517.
    STRINGi 9606.ENSP00000261438.

    PTM databases

    PhosphoSitei P57682.

    Polymorphism databases

    DMDMi 12644533.

    Proteomic databases

    MaxQBi P57682.
    PaxDbi P57682.
    PRIDEi P57682.

    Protocols and materials databases

    DNASUi 51274.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261438 ; ENSP00000261438 ; ENSG00000109787 . [P57682-1 ]
    ENST00000514033 ; ENSP00000421252 ; ENSG00000109787 . [P57682-2 ]
    GeneIDi 51274.
    KEGGi hsa:51274.
    UCSCi uc003gtg.2. human. [P57682-1 ]

    Organism-specific databases

    CTDi 51274.
    GeneCardsi GC04P038668.
    HGNCi HGNC:16516. KLF3.
    HPAi HPA049512.
    MIMi 609392. gene.
    neXtProti NX_P57682.
    PharmGKBi PA30137.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000232138.
    HOVERGENi HBG003941.
    InParanoidi P57682.
    KOi K15605.
    OMAi NSSMQVP.
    OrthoDBi EOG747PJ4.
    PhylomeDBi P57682.
    TreeFami TF350556.

    Miscellaneous databases

    GeneWikii KLF3.
    GenomeRNAii 51274.
    NextBioi 54481.
    PROi P57682.
    SOURCEi Search...

    Gene expression databases

    Bgeei P57682.
    CleanExi HS_KLF3.
    Genevestigatori P57682.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Matsumoto N., Yoshida T., Terada M.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "cDNA cloning, subcellular localization and tissue expression of a new human Kruppel-like transcription factor: human basic Kruppel-like factor (hBKLF)."
      Wang M.J., Qu X.H., Wang L.S., Zhai Y., Wu S.L., He F.C.
      Yi Chuan Xue Bao 30:1-9(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Hippocampus.
    4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiKLF3_HUMAN
    AccessioniPrimary (citable) accession number: P57682
    Secondary accession number(s): Q6PIR1, Q86TN0, Q9P2X6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3