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P57682

- KLF3_HUMAN

UniProt

P57682 - KLF3_HUMAN

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Protein
Krueppel-like factor 3
Gene
KLF3, BKLF
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri260 – 28425C2H2-type 1
Add
BLAST
Zinc fingeri290 – 31425C2H2-type 2
Add
BLAST
Zinc fingeri320 – 34223C2H2-type 3
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. cellular response to peptide Source: Ensembl
  2. multicellular organismal development Source: ProtInc
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Krueppel-like factor 3
Alternative name(s):
Basic krueppel-like factor
CACCC-box-binding protein BKLF
TEF-2
Gene namesi
Name:KLF3
Synonyms:BKLF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:16516. KLF3.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30137.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Krueppel-like factor 3
PRO_0000047165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki10 – 10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Modified residuei92 – 921Phosphoserine2 Publications
Modified residuei101 – 1011Phosphoserine1 Publication
Modified residuei111 – 1111Phosphoserine1 Publication
Cross-linki198 – 198Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Modified residuei250 – 2501Phosphoserine1 Publication

Post-translational modificationi

Sumoylated with SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site By similarity.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP57682.
PaxDbiP57682.
PRIDEiP57682.

PTM databases

PhosphoSiteiP57682.

Expressioni

Gene expression databases

BgeeiP57682.
CleanExiHS_KLF3.
GenevestigatoriP57682.

Organism-specific databases

HPAiHPA049512.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

BioGridi119426. 5 interactions.
IntActiP57682. 3 interactions.
MINTiMINT-4786517.
STRINGi9606.ENSP00000261438.

Structurei

3D structure databases

ProteinModelPortaliP57682.
SMRiP57682. Positions 251-345.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7474Repressor domain
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 655CTBP-binding motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 251251Pro-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
HOGENOMiHOG000232138.
HOVERGENiHBG003941.
InParanoidiP57682.
KOiK15605.
OMAiNSSMQVP.
OrthoDBiEOG747PJ4.
PhylomeDBiP57682.
TreeFamiTF350556.

Family and domain databases

Gene3Di3.30.160.60. 3 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P57682-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLMFDPVPVK QEAMDPVSVS YPSNYMESMK PNKYGVIYST PLPEKFFQTP    50
EGLSHGIQME PVDLTVNKRS SPPSAGNSPS SLKFPSSHRR ASPGLSMPSS 100
SPPIKKYSPP SPGVQPFGVP LSMPPVMAAA LSRHGIRSPG ILPVIQPVVV 150
QPVPFMYTSH LQQPLMVSLS EEMENSSSSM QVPVIESYEK PISQKKIKIE 200
PGIEPQRTDY YPEEMSPPLM NSVSPPQALL QENHPSVIVQ PGKRPLPVES 250
PDTQRKRRIH RCDYDGCNKV YTKSSHLKAH RRTHTGEKPY KCTWEGCTWK 300
FARSDELTRH FRKHTGIKPF QCPDCDRSFS RSDHLALHRK RHMLV 345
Length:345
Mass (Da):38,829
Last modified:December 1, 2000 - v1
Checksum:iA490D642AB8F1FDC
GO
Isoform 2 (identifier: P57682-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     233-345: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Show »
Length:232
Mass (Da):25,430
Checksum:i20A838F91F92DD50
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti207 – 2071R → S.
Corresponds to variant rs17616226 [ dbSNP | Ensembl ].
VAR_052715

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei233 – 345113Missing in isoform 2.
VSP_014532Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851P → R in AAH51687. 1 Publication
Sequence conflicti116 – 1161P → H in AAH51687. 1 Publication
Sequence conflicti305 – 3051D → G in AAH30662. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024523 mRNA. Translation: BAA92271.1.
AF285837 mRNA. Translation: AAK27329.1.
BC030662 mRNA. Translation: AAH30662.1.
BC051687 mRNA. Translation: AAH51687.1.
CCDSiCCDS3444.1. [P57682-1]
RefSeqiNP_057615.3. NM_016531.5. [P57682-1]
UniGeneiHs.298658.

Genome annotation databases

EnsembliENST00000261438; ENSP00000261438; ENSG00000109787. [P57682-1]
ENST00000514033; ENSP00000421252; ENSG00000109787. [P57682-2]
GeneIDi51274.
KEGGihsa:51274.
UCSCiuc003gtg.2. human. [P57682-1]

Polymorphism databases

DMDMi12644533.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024523 mRNA. Translation: BAA92271.1 .
AF285837 mRNA. Translation: AAK27329.1 .
BC030662 mRNA. Translation: AAH30662.1 .
BC051687 mRNA. Translation: AAH51687.1 .
CCDSi CCDS3444.1. [P57682-1 ]
RefSeqi NP_057615.3. NM_016531.5. [P57682-1 ]
UniGenei Hs.298658.

3D structure databases

ProteinModelPortali P57682.
SMRi P57682. Positions 251-345.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119426. 5 interactions.
IntActi P57682. 3 interactions.
MINTi MINT-4786517.
STRINGi 9606.ENSP00000261438.

PTM databases

PhosphoSitei P57682.

Polymorphism databases

DMDMi 12644533.

Proteomic databases

MaxQBi P57682.
PaxDbi P57682.
PRIDEi P57682.

Protocols and materials databases

DNASUi 51274.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261438 ; ENSP00000261438 ; ENSG00000109787 . [P57682-1 ]
ENST00000514033 ; ENSP00000421252 ; ENSG00000109787 . [P57682-2 ]
GeneIDi 51274.
KEGGi hsa:51274.
UCSCi uc003gtg.2. human. [P57682-1 ]

Organism-specific databases

CTDi 51274.
GeneCardsi GC04P038668.
HGNCi HGNC:16516. KLF3.
HPAi HPA049512.
MIMi 609392. gene.
neXtProti NX_P57682.
PharmGKBi PA30137.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5048.
HOGENOMi HOG000232138.
HOVERGENi HBG003941.
InParanoidi P57682.
KOi K15605.
OMAi NSSMQVP.
OrthoDBi EOG747PJ4.
PhylomeDBi P57682.
TreeFami TF350556.

Miscellaneous databases

GeneWikii KLF3.
GenomeRNAii 51274.
NextBioi 54481.
PROi P57682.
SOURCEi Search...

Gene expression databases

Bgeei P57682.
CleanExi HS_KLF3.
Genevestigatori P57682.

Family and domain databases

Gene3Di 3.30.160.60. 3 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 3 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Matsumoto N., Yoshida T., Terada M.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "cDNA cloning, subcellular localization and tissue expression of a new human Kruppel-like transcription factor: human basic Kruppel-like factor (hBKLF)."
    Wang M.J., Qu X.H., Wang L.S., Zhai Y., Wu S.L., He F.C.
    Yi Chuan Xue Bao 30:1-9(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Hippocampus.
  4. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-250, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-101, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiKLF3_HUMAN
AccessioniPrimary (citable) accession number: P57682
Secondary accession number(s): Q6PIR1, Q86TN0, Q9P2X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi