P57655 (ILVC_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BU599

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151286
Sites
	Active site	131	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P57655 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 0C108D0AF1476504	FASTA	490	55,828
	10 20 30 40 50 60 MNYFNTLNFT QKINQINKCR FMKKEEFNKK NDILKNKNIV IVGCGAQGLN QGLNMRDAGL 70 80 90 100 110 120 NISYALKKNS IANKNQSWIN AIENNFKVDD YDALIPDADL VINLTPDKQH HSVIKKLQKL 130 140 150 160 170 180 MKKNAVLGYS HGFNIVEFGE KIRKDITVIM VAPKCPGTEV REEYKRGFGV PTLIAVHHEN 190 200 210 220 230 240 DINKIGLEVA KAWAFSTGGH RAGVLESSFI AEVKSDLMGE QTILCGMLQT ASLLCYEKLI 250 260 270 280 290 300 TEKCNPAYSA KLIQNGWETI TESLKHGGIT LMMDRLSNSS KIRAYKLSKE IKKILSPLFQ 310 320 330 340 350 360 KHMDDIISGE FSNEMMKDWE NQDLKLLNWR YKTKNTSFET APVYNEKIPE QEYYDHGILM 370 380 390 400 410 420 IAILKSGIEL SFEKMIETGI KEESAYYESL HELPLIANTI ARKKLYEMNK VISDTAEYGS 430 440 450 460 470 480 YLFSESAYPI LKEFISTLNK SDLGCALSHQ SVNNIELYRI NQKIQNHPIE IIGCTLRNYM 490 KKMKAITVAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
[2]	"Accelerated evolutionary rates at biosynthetic loci of Buchnera-Uroleucon." Wernegreen J.J., Moran N.A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-412.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13284.1. AF217558 Genomic DNA. Translation: AAK01029.1.
RefSeq	NP_240398.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57655.
SMR	P57655. Positions 2-488.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002783; EBBUCP00000002559; EBBUCG00000002783.
GeneID	1109935.
GenomeReviews	Gene locus BU599 in contig BA000003_GR.
KEGG	buc:BU599.
PATRIC	21244750. VBIBucAph127364_0602.
Phylogenomic databases
GeneTree	EBGT00050000007925.
HOGENOM	HBG297205.
OMA	EKHMDDI.
PhylomeDB	P57655.
ProtClustDB	PRK05225.
Enzyme and pathway databases
BioCyc	BSP107806:BU599-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. IPR014359. KetolA_reductoisomerase_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit. PTHR21371:SF2. PTHR21371:SF2. 1 hit.
Pfam	PF01450. IlvC. 2 hits. PF07991. IlvN. 1 hit. [Graphical view]
PIRSF	PIRSF000116. IlvC_gammaproteo. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 2 hits.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BUCAI

Accession

Primary (citable) accession number: P57655
Secondary accession number(s): Q9AQ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program