ID   AMIB_BUCAI              Reviewed;         237 AA.
AC   P57638;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2001, sequence version 1.
DT   27-MAR-2024, entry version 94.
DE   RecName: Full=Putative N-acetylmuramoyl-L-alanine amidase;
DE            EC=3.5.1.28;
GN   Name=amiB; OrderedLocusNames=BU576;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Cell-wall hydrolase involved in septum cleavage during cell
CC       division. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-
CC         amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
CC       {ECO:0000305}.
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DR   EMBL; BA000003; BAB13265.1; -; Genomic_DNA.
DR   RefSeq; NP_240379.1; NC_002528.1.
DR   RefSeq; WP_009874524.1; NC_002528.1.
DR   AlphaFoldDB; P57638; -.
DR   SMR; P57638; -.
DR   EnsemblBacteria; BAB13265; BAB13265; BAB13265.
DR   KEGG; buc:BU576; -.
DR   PATRIC; fig|107806.10.peg.580; -.
DR   eggNOG; COG0860; Bacteria.
DR   HOGENOM; CLU_014322_4_1_6; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR   CDD; cd02696; MurNAc-LAA; 1.
DR   Gene3D; 3.40.630.40; Zn-dependent exopeptidases; 1.
DR   InterPro; IPR002508; MurNAc-LAA_cat.
DR   PANTHER; PTHR30404; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; 1.
DR   PANTHER; PTHR30404:SF6; N-ACETYLMURAMOYL-L-ALANINE AMIDASE AMIB; 1.
DR   Pfam; PF01520; Amidase_3; 1.
DR   SMART; SM00646; Ami_3; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Hydrolase; Reference proteome; Secreted.
FT   CHAIN           1..237
FT                   /note="Putative N-acetylmuramoyl-L-alanine amidase"
FT                   /id="PRO_0000164421"
FT   DOMAIN          7..225
FT                   /note="MurNAc-LAA"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   237 AA;  27240 MW;  D8FD528FB89C406C CRC64;
     MSKKITILID AGHGGYDPGA IGIRGLKEKN INIEIALKLE KLLNHDKMFC TILTRHNDSY
     LSLKKRKQLL KKNQVNFLIS IHADSSRKQN VSGASIWIVS KTRINREINN YLKNKSTLLF
     SKKIENIFKQ NKNDFFLKKT ILDLQSNNFQ KIELDLSKEI LKQLEKNTKL NKKYPNYASL
     GILSSINTPS ILIETGFITN ILEGKKLKTT NYQNKIANSI YLGLKNYFTK SSYILKK
//