Reviewed, UniProtKB/Swiss-Prot P57635 (PTM3C_BUCAI)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICBA component
Alternative name(s):
    EIICBA-Mtl
      Short name=EII-Mtl
Including the following 3 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component
    3- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system mannitol-specific EIIA component

Gene names

Name:	mtlA
Ordered Locus Names:	BU572

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Post-translational modification	An intramolecular phosphotransfer takes places between His-548 and Cys-380 By similarity.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	PTS system mannitol-specific EIICBA component		PRO_0000186611
Regions
	Transmembrane	20 – 40	21	Potential
	Transmembrane	52 – 72	21	Potential
	Transmembrane	86 – 106	21	Potential
	Transmembrane	134 – 154	21	Potential
	Transmembrane	161 – 181	21	Potential
	Transmembrane	213 – 233	21	Potential
	Transmembrane	269 – 289	21	Potential
	Transmembrane	313 – 333	21	Potential
	Domain	12 – 341	330	PTS EIIC type-2
	Domain	374 – 469	96	PTS EIIB type-2
	Domain	488 – 630	143	PTS EIIA type-2
Sites
	Active site	380	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	548	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57635-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: DC413636A0BFF552	FASTA	632	69,974
	10 20 30 40 50 60 MLTLIKLKIQ NFGRFLSNMI MPNISIFIAW GMMNALFMPL GWQPNKTLEQ LISPMIFYLL 70 80 90 100 110 120 PILIGYTGGS LISGSRGGLI GSITTIGVIT STNIPMLLGA MISGPLGGWT IKYFDKKIEN 130 140 150 160 170 180 KVKNGFEMLV NNFSLAILGI LLAIISFFTI GPFIEWVSYF LGTLIQIILS YNLLPLTSII 190 200 210 220 230 240 IEPAKIFFLN NVINHGIFSP LGIQDALDKN HSIFFLIESN PGPGLGLLIA WFFFGKGELS 250 260 270 280 290 300 KSSGGAALIE FFGGIHEIYF PYVLIKPKLI IPLILGGMSG IFILVLLHGG LISTASPGSI 310 320 330 340 350 360 LSILAMTPKG LYFSNIISVA CSFLVSFISS SILLKYDFNT IQKDSNYTVK QGKNSFNSKT 370 380 390 400 410 420 SREDFNNFNF SKIKTIIVAC DAGMGSSAMG ASILRKKIKN ANLNHISVLN MAINALPQDA 430 440 450 460 470 480 DLIITHQNLT NRAKDNAPFS QHISLKNFLN NHFYDNLVKK LVENMVFLYD NHTDSVNKYT 490 500 510 520 530 540 QQKKDALFQL NEENIILNQY ASNKEEAINI VGKHLVKQGY VKFDYIDSML EREKMASTWL 550 560 570 580 590 600 GESIALPHGT IEAKDSVLKT GIIFCQFPKG VRFGEDVDDI AYLVIGIAAK NNEHIMVVSN 610 620 630 ITNALDKKDT IQRLSHTTSI KEALSLLTME KI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13262.1.
RefSeq	NP_240376.1.
3D structure databases
HSSP	HSSP built from PDB template 1A3A based on UniProtKB P00550.
ModBase	Search...
Genome annotation databases
GeneID	1109912.
GenomeReviews	Gene locus BU572 in contig BA000003_GR.
KEGG	buc:BU572.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57635.
OMA	ITHKDLT.
Enzyme and pathway databases
BioCyc	BSP107806:BU572-MON.
Family and domain databases
InterPro	IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003501. PTS_IIB_lac. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
ProDom	PD001689. PTS_EIIA_2. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTM3C_BUCAI

Accession

Primary (citable) accession number: P57635

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)