P57624 (CYSQ_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3'(2'),5'-bisphosphate nucleotidase CysQ
EC=3.1.3.7

Alternative name(s):
3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
3'-phosphoadenosine 5'-phosphate phosphatase
Short name=PAP phosphatase
DPNPase

Gene names

Name:	cysQ
Ordered Locus Names:	BU561

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May also convert adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) By similarity.
Catalytic activity	Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.
Cofactor	Magnesium By similarity.
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'(2'),5'-bisphosphate nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	3'(2'),5'-bisphosphate nucleotidase CysQ		PRO_0000142548
Regions
	Region	101 – 104	4	Substrate binding By similarity
Sites
	Metal binding	80	1	Magnesium 1 By similarity
	Metal binding	99	1	Magnesium 1 By similarity
	Metal binding	99	1	Magnesium 2 By similarity
	Metal binding	101	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	102	1	Magnesium 2 By similarity
	Metal binding	222	1	Magnesium 2 By similarity
	Binding site	80	1	Substrate By similarity
	Binding site	222	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57624 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 2C3E30CE563FF613	FASTA	265	29,943
	10 20 30 40 50 60 MQKINFFDTI NNIWRKMLDK ICQLARSAGH CIMKLYNSQK FINVSYKPDN TPITDVDYAA 70 80 90 100 110 120 NNIIKKGLSL ISPQIPIISE EESYNFEICR NWNSYWLVDP LDGTKEFLKK NGEFTVNISL 130 140 150 160 170 180 IEYGVPILGV IYAPFFDVLY SAFYDNAWKE EKFGSKKKIN VAQAEAPLLV ISRSHPNETL 190 200 210 220 230 240 KNYLEKIKCC KIKKMGSSLK FCLIAEGTAQ IYPRFGDTHI WDTAAGHAIV IAAGGKVQTW 250 260 TGGNLNYSLS SRVSFINSGF YASAL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13251.1.
RefSeq	NP_240365.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57624.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003282; EBBUCP00000003058; EBBUCG00000003282.
GeneID	1109901.
GenomeReviews	Gene locus BU561 in contig BA000003_GR.
KEGG	buc:BU561.
PATRIC	21244666. VBIBucAph127364_0564.
Phylogenomic databases
GeneTree	EBGT00050000007980.
HOGENOM	HBG730251.
OMA	CRIAEGG.
PhylomeDB	P57624.
ProtClustDB	CLSK2459981.
Enzyme and pathway databases
BioCyc	BSP107806:BU561-MONOMER.
Family and domain databases
InterPro	IPR006240. Bisphos_bac. IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
KO	K01082.
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES.
TIGRFAMs	TIGR01331. Bisphos_cysQ. 1 hit.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSQ_BUCAI

Accession

Primary (citable) accession number: P57624

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program