Reviewed, UniProtKB/Swiss-Prot P57622 (PYRE_BUCAI)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BU559

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110681
Regions
	Region	33 – 34	2	Orotate binding By similarity
	Region	71 – 72	2	5-phosphoribose 1-diphosphate binding By similarity
	Region	124 – 132	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	25	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	98	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	99	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	102	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	104	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	128	1	Orotate By similarity
	Binding site	156	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57622-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 5CBA86CA73D4B6BE	FASTA	213	24,610
	10 20 30 40 50 60 MDWKKEFIDF SFKKKVLKFG VFQLKSGRIS PYFFNSGLLS TGIDIIKIGL FYARSIIDSK 70 80 90 100 110 120 NKFDVLFGPA YKGIPIAVAT SIALKNHYNL NVPYSFNRKE YKEHGEKGDL IGSTIYKKRV 130 140 150 160 170 180 IILDDVITSG TAIHHSIKII EKQEASISSI FVLLDRKEKG IRKLSTINHF RNQKSYKIIS 190 200 210 IITIDDLIEY VLEDKKLKEH IPQLIKYREK YGI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13249.1.
RefSeq	NP_240363.1.
3D structure databases
SMR	P57622. Positions 1-213.
ModBase	Search...
Genome annotation databases
GeneID	1109899.
GenomeReviews	Gene locus BU559 in contig BA000003_GR.
KEGG	buc:BU559.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	YKGITLA.
PhylomeDB	P57622.
Enzyme and pathway databases
BioCyc	BSP107806:BU559-MONOMER.
Family and domain databases
HAMAP	MF_01208_B. PyrE_B. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BUCAI

Accession

Primary (citable) accession number: P57622

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)