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P57622 (PYRE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE
Ordered Locus Names:BU559
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_0000110681

Regions

Region33 – 342Orotate binding By similarity
Region71 – 7225-phosphoribose 1-diphosphate binding By similarity
Region124 – 13295-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site2515-phosphoribose 1-diphosphate By similarity
Binding site9815-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site9915-phosphoribose 1-diphosphate By similarity
Binding site10215-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10415-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1281Orotate By similarity
Binding site1561Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
P57622 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 5CBA86CA73D4B6BE

FASTA21324,610
        10         20         30         40         50         60 
MDWKKEFIDF SFKKKVLKFG VFQLKSGRIS PYFFNSGLLS TGIDIIKIGL FYARSIIDSK 

        70         80         90        100        110        120 
NKFDVLFGPA YKGIPIAVAT SIALKNHYNL NVPYSFNRKE YKEHGEKGDL IGSTIYKKRV 

       130        140        150        160        170        180 
IILDDVITSG TAIHHSIKII EKQEASISSI FVLLDRKEKG IRKLSTINHF RNQKSYKIIS 

       190        200        210 
IITIDDLIEY VLEDKKLKEH IPQLIKYREK YGI

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed: 10993077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB13249.1.
RefSeqNP_240363.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57622.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBUCT00000003220; EBBUCP00000002996; EBBUCG00000003220.
GeneID1109899.
GenomeReviewsGene locus BU559 in contig BA000003_GR.
KEGGbuc:BU559.
PATRIC21244662. VBIBucAph127364_0562.

Phylogenomic databases

GeneTreeEBGT00050000007851.
HOGENOMHBG404341.
OMAFAFNRKE.
PhylomeDBP57622.
ProtClustDBPRK00455.

Enzyme and pathway databases

BioCycBSP107806:BU559-MONOMER.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
KOK00762.
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_BUCAI
AccessionPrimary (citable) accession number: P57622
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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