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P57612

- GSHB_BUCAI

UniProt

P57612 - GSHB_BUCAI

Protein

Glutathione synthetase

Gene

gshB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi286 – 2861Magnesium or manganeseUniRule annotation
    Metal bindingi288 – 2881Magnesium or manganeseUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi156 – 21257ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutathione synthase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-547-MONOMER.
    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshBUniRule annotation
    Ordered Locus Names:BU547
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320Glutathione synthetasePRO_0000197459Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP57612.
    SMRiP57612. Positions 7-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini130 – 315186ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic GSH synthase family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0189.
    HOGENOMiHOG000265022.
    KOiK01920.
    OMAiDWKIARA.
    OrthoDBiEOG6WMHWB.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_00162. GSH_S.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01380. glut_syn. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57612-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKKISLKIG ILMDSIESIN IKKDSSFAIL LEAQKRNHII HYMEMNDLYL    50
    RKEQAFARTR LIKLIKNTQK WYQFIKQQSI SLSELDVILM RKDPPFNTEF 100
    IYSTYILERA EETGVLIINK PKSLRDCNEK IFTSWFPDLI TDTLVTRNIF 150
    QIRQFWEKYQ DIIIKPLDAM GGANIFRIKK NDPNFSVIVE NMTNYERKYC 200
    MVQNYLPEIK LGDKRILIIN GKPIPWCVAR IARIGETRAN LAAGGEGKIQ 250
    SLSETDWKIA NYLSPTLKKR GLILVGLDVI GDKLTEINVT SPTCICEIES 300
    QKNISITGML LDYIEKKVCL 320
    Length:320
    Mass (Da):37,022
    Last modified:December 1, 2000 - v1
    Checksum:iF4FF1B99F3FDC4A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13239.1.
    RefSeqiNP_240353.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13239; BAB13239; BAB13239.
    GeneIDi1109889.
    KEGGibuc:BU547.
    PATRICi21244636. VBIBucAph127364_0551.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13239.1 .
    RefSeqi NP_240353.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57612.
    SMRi P57612. Positions 7-318.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13239 ; BAB13239 ; BAB13239 .
    GeneIDi 1109889.
    KEGGi buc:BU547.
    PATRICi 21244636. VBIBucAph127364_0551.

    Phylogenomic databases

    eggNOGi COG0189.
    HOGENOMi HOG000265022.
    KOi K01920.
    OMAi DWKIARA.
    OrthoDBi EOG6WMHWB.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .
    BioCyci BAPH107806:GBZJ-547-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_00162. GSH_S.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01380. glut_syn. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiGSHB_BUCAI
    AccessioniPrimary (citable) accession number: P57612
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3