P57605 (AROK_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate kinase
Short name=SK
EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	BU539

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm Probable HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW shikimate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 173	173	Shikimate kinase HAMAP MF_00109		PRO_0000192369
Regions
	Nucleotide binding	14 – 19	6	ATP By similarity
Sites
	Metal binding	18	1	Magnesium By similarity
	Binding site	36	1	Substrate By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	82	1	Substrate; via amide nitrogen By similarity
	Binding site	120	1	ATP By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	157	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57605 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: CB3B64A15C1A4C02	FASTA	173	19,764
	10 20 30 40 50 60 MAEKRNIFLV GPMGAGKSTI GRQLSQQLNM EFFDSDQEIE KRTGADISWV FDVEGESGFR 70 80 90 100 110 120 LREQRVIDEL TTKQGIVLAT GGGSVKFRET RNFLSSRGIV VYLETTIEKQ LARTKRDKKR 130 140 150 160 170 PLLQNADSNR MILENLAYER NPFYEEIADI KVKTDDQSAK SVAFNIIRLL EEI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13232.1.
RefSeq	NP_240346.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57605.
SMR	P57605. Positions 3-171.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003084; EBBUCP00000002860; EBBUCG00000003084.
GeneID	1109882.
GenomeReviews	Gene locus BU539 in contig BA000003_GR.
KEGG	buc:BU539.
PATRIC	21244620. VBIBucAph127364_0544.
Phylogenomic databases
GeneTree	EBGT00050000007771.
HOGENOM	HBG335815.
OMA	VIYLRAN.
PhylomeDB	P57605.
ProtClustDB	PRK05057.
Enzyme and pathway databases
BioCyc	BSP107806:BU539-MONOMER.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shikimate_kinase. IPR023000. Shikimate_kinase_CS. [Graphical view]
KO	K00891.
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BUCAI

Accession

Primary (citable) accession number: P57605

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program