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Protein

3-dehydroquinate synthase

Gene

aroB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) to dehydroquinate (DHQ).UniRule annotation

Catalytic activityi

3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • NAD+UniRule annotation
  • Co2+UniRule annotation, Zn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit. Can use either Co2+ or Zn2+.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145NAD; via carbonyl oxygenUniRule annotation1
Binding sitei154NADUniRule annotation1
Metal bindingi187Cobalt or zincUniRule annotation1
Metal bindingi250Cobalt or zinc; via tele nitrogenUniRule annotation1
Metal bindingi267Cobalt or zinc; via tele nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 79NADUniRule annotation6
Nucleotide bindingi108 – 112NADUniRule annotation5
Nucleotide bindingi132 – 133NADUniRule annotation2
Nucleotide bindingi172 – 175NADUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandCobalt, Metal-binding, NAD, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00053; UER00085.

Names & Taxonomyi

Protein namesi
Recommended name:
3-dehydroquinate synthaseUniRule annotation (EC:4.2.3.4UniRule annotation)
Short name:
DHQSUniRule annotation
Gene namesi
Name:aroBUniRule annotation
Ordered Locus Names:BU538
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001407171 – 3633-dehydroquinate synthaseAdd BLAST363

Interactioni

Protein-protein interaction databases

STRINGi107806.BU538.

Structurei

3D structure databases

ProteinModelPortaliP57604.
SMRiP57604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105D49. Bacteria.
COG0337. LUCA.
HOGENOMiHOG000007970.
KOiK01735.
OMAiIKMAVCF.

Family and domain databases

HAMAPiMF_00110. DHQ_synthase. 1 hit.
InterProiView protein in InterPro
IPR016037. DHQ_synth_AroB.
IPR030963. DHQ_synth_fam.
IPR030960. DHQS/DOIS.
PfamiView protein in Pfam
PF01761. DHQ_synthase. 1 hit.
PIRSFiPIRSF001455. DHQ_synth. 1 hit.
TIGRFAMsiTIGR01357. aroB. 1 hit.

Sequencei

Sequence statusi: Complete.

P57604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIVERLQVV LGERSYPISI GAGIIQEDDI FWPLKPGDQA MLVTNKTLAN
60 70 80 90 100
LLKDKVFYHL RKSGIKIDQV ILSDGEQYKT LNEMELIISA LLEKKHARDT
110 120 130 140 150
TLIALGGGVI GDLAGFAASV YQRGVRFIQI PTTLLSQVDA SVGGKTAVNH
160 170 180 190 200
LLGKNMIGSF WQPSSVIIDI DCLKTLPYNE LVSGMAEVIK YAIVFDKTFF
210 220 230 240 250
CWLEENIESI LSLNHTAMSY CIKKCCELKS QLIALDEREN NLRALLNLGH
260 270 280 290 300
TYGHAIEVHA GYGNWLHGEA ISVGMVMAAR TSELLGHLKT IDFKRILVLL
310 320 330 340 350
KRTGLPIKGP KNMSAASYLP YMMRDKKVIS GEMRLVLPLS IGKAEIYSNI
360
DKNIILTAIK HSQ
Length:363
Mass (Da):40,297
Last modified:December 1, 2000 - v1
Checksum:i1DB8EC7B8D8F6EC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13231.1.
RefSeqiNP_240345.1. NC_002528.1.
WP_009874489.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13231; BAB13231; BAB13231.
GeneIDi1109881.
KEGGibuc:BU538.
PATRICifig|107806.10.peg.543.

Similar proteinsi

Entry informationi

Entry nameiAROB_BUCAI
AccessioniPrimary (citable) accession number: P57604
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: August 30, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families