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P57596 (TUSB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein TusB
Alternative name(s):
tRNA 2-thiouridine synthesizing protein B
Gene names
Name:tusB
Ordered Locus Names:BU530
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length95 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions By similarity. HAMAP-Rule MF_01564

Subunit structure

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01564.

Sequence similarities

Belongs to the DsrH/TusB family.

Sequence caution

The sequence BAB13223.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA wobble position uridine thiolation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9595Protein TusB HAMAP-Rule MF_01564
PRO_0000216259

Sequences

Sequence LengthMass (Da)Tools
P57596 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 831FAB631BBB7382

FASTA9511,097
        10         20         30         40         50         60 
MLHTLMKSPF ETNVSLVISM LKKSDDFLAL QDGVLIALKD NIFLKSIIMS PVKLYLIKED 

        70         80         90 
VYARGIRKNI SREFILINYI HFVSLTLKHK KQMTW 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13223.1. Different initiation.
RefSeqNP_240337.2. NC_002528.1.

3D structure databases

ProteinModelPortalP57596.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13223; BAB13223; BAB13223.
GeneID1109873.
KEGGbuc:BU530.
PATRIC21244602. VBIBucAph127364_0535.

Phylogenomic databases

eggNOGCOG2168.
HOGENOMHOG000284333.
KOK07237.
OMAEFVQLTV.
OrthoDBEOG6MWNF1.
ProtClustDBPRK13510.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-530-MONOMER.

Family and domain databases

Gene3D3.40.1260.10. 1 hit.
HAMAPMF_01564. Thiourid_synth_B.
InterProIPR027396. DsrEFH-like.
IPR023526. Sulphur_relay_TusB.
IPR007215. Sulphur_relay_TusB/DsrH.
[Graphical view]
PfamPF04077. DsrH. 1 hit.
[Graphical view]
SUPFAMSSF75169. SSF75169. 1 hit.
TIGRFAMsTIGR03011. sulf_tusB_dsrH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTUSB_BUCAI
AccessionPrimary (citable) accession number: P57596
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 16, 2006
Last modified: February 19, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names