P57596 (TUSB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein TusB

Alternative name(s):
tRNA 2-thiouridine synthesizing protein B

Gene names

Name:	tusB
Ordered Locus Names:	BU530

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions By similarity. HAMAP-Rule MF_01564
Subunit structure	Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DsrH/TusB family.
Sequence caution	The sequence BAB13223.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA wobble position uridine thiolation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 95	95	Protein TusB HAMAP-Rule MF_01564		PRO_0000216259

Sequences

	Sequence		Length	Mass (Da)
	P57596 [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 831FAB631BBB7382	FASTA	95	11,097
	10 20 30 40 50 60 MLHTLMKSPF ETNVSLVISM LKKSDDFLAL QDGVLIALKD NIFLKSIIMS PVKLYLIKED 70 80 90 VYARGIRKNI SREFILINYI HFVSLTLKHK KQMTW

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13223.1. Different initiation.
RefSeq	NP_240337.2. NC_002528.1.
3D structure databases
ProteinModelPortal	P57596.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13223; BAB13223; BAB13223.
GeneID	1109873.
KEGG	buc:BU530.
PATRIC	21244602. VBIBucAph127364_0535.
Phylogenomic databases
eggNOG	COG2168.
HOGENOM	HOG000284333.
KO	K07237.
OMA	EFVQLTV.
ProtClustDB	PRK13510.
Family and domain databases
HAMAP	MF_01564. Thiourid_synth_B.
InterPro	IPR023526. Sulphur_relay_TusB. IPR007215. Sulphur_relay_TusB/DsrH. [Graphical view]
Pfam	PF04077. DsrH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03011. sulf_tusB_dsrH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TUSB_BUCAI

Accession

Primary (citable) accession number: P57596

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 16, 2006
Last modified:	May 1, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program