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Protein

Protein TusB

Gene

tusB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions.UniRule annotation

GO - Biological processi

  1. tRNA wobble position uridine thiolation Source: InterPro
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TusBUniRule annotation
Alternative name(s):
tRNA 2-thiouridine synthesizing protein BUniRule annotation
Gene namesi
Name:tusBUniRule annotation
Ordered Locus Names:BU530
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Protein TusBPRO_0000216259Add
BLAST

Interactioni

Subunit structurei

Heterohexamer, formed by a dimer of trimers. The hexameric TusBCD complex contains 2 copies each of TusB, TusC and TusD. The TusBCD complex interacts with TusE.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the DsrH/TusB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2168.
HOGENOMiHOG000284333.
KOiK07237.
OMAiVYALQED.
OrthoDBiEOG6MWNF1.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_01564. Thiourid_synth_B.
InterProiIPR027396. DsrEFH-like.
IPR023526. Sulphur_relay_TusB.
IPR007215. Sulphur_relay_TusB/DsrH.
[Graphical view]
PfamiPF04077. DsrH. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03011. sulf_tusB_dsrH. 1 hit.

Sequencei

Sequence statusi: Complete.

P57596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHTLMKSPF ETNVSLVISM LKKSDDFLAL QDGVLIALKD NIFLKSIIMS
60 70 80 90
PVKLYLIKED VYARGIRKNI SREFILINYI HFVSLTLKHK KQMTW
Length:95
Mass (Da):11,097
Last modified:May 16, 2006 - v2
Checksum:i831FAB631BBB7382
GO

Sequence cautioni

The sequence BAB13223.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13223.1. Different initiation.
RefSeqiNP_240337.2. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13223; BAB13223; BAB13223.
GeneIDi1109873.
KEGGibuc:BU530.
PATRICi21244602. VBIBucAph127364_0535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13223.1. Different initiation.
RefSeqiNP_240337.2. NC_002528.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13223; BAB13223; BAB13223.
GeneIDi1109873.
KEGGibuc:BU530.
PATRICi21244602. VBIBucAph127364_0535.

Phylogenomic databases

eggNOGiCOG2168.
HOGENOMiHOG000284333.
KOiK07237.
OMAiVYALQED.
OrthoDBiEOG6MWNF1.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-530-MONOMER.

Family and domain databases

Gene3Di3.40.1260.10. 1 hit.
HAMAPiMF_01564. Thiourid_synth_B.
InterProiIPR027396. DsrEFH-like.
IPR023526. Sulphur_relay_TusB.
IPR007215. Sulphur_relay_TusB/DsrH.
[Graphical view]
PfamiPF04077. DsrH. 1 hit.
[Graphical view]
SUPFAMiSSF75169. SSF75169. 1 hit.
TIGRFAMsiTIGR03011. sulf_tusB_dsrH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiTUSB_BUCAI
AccessioniPrimary (citable) accession number: P57596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 16, 2006
Last modified: April 1, 2015
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.