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P57566 (RPOA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit alpha

Short name=RNAP subunit alpha
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha
Gene names
Name:rpoA
Ordered Locus Names:BU499
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_00059

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_00059

Subunit structure

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Domain

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity. HAMAP-Rule MF_00059

Sequence similarities

Belongs to the RNA polymerase alpha chain family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329DNA-directed RNA polymerase subunit alpha HAMAP-Rule MF_00059
PRO_0000175279

Regions

Region1 – 235235Alpha N-terminal domain (alpha-NTD) By similarity
Region249 – 32981Alpha C-terminal domain (alpha-CTD) By similarity

Sequences

Sequence LengthMass (Da)Tools
P57566 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 8340F07893FF0D85

FASTA32936,855
        10         20         30         40         50         60 
MQNSIMDFLR PRLVDIEQIS ATHTKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE 

        70         80         90        100        110        120 
IDGVLHEYST KEGVQEDVLE ILLNLKALAV KVYGKDETLL TLHKSGIGSI TAADIIHDGD 

       130        140        150        160        170        180 
VEIIKPQHVI CHLTDENASI KMRIKVQRGR GYIPASSRVH LEEDSRPIGC LLVDACYSPI 

       190        200        210        220        230        240 
NRISYNVEAA RVEQRTDLDK LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDIREP 

       250        260        270        280        290        300 
EFKEEKPEFE PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL 

       310        320 
TEIKDVLASR SLSLGMRLEN WPPSSILDE 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13192.1.
RefSeqNP_240306.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57566.
SMRP57566. Positions 1-235, 249-329.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP57566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13192; BAB13192; BAB13192.
GeneID1109842.
KEGGbuc:BU499.
PATRIC21244540. VBIBucAph127364_0504.

Phylogenomic databases

eggNOGCOG0202.
HOGENOMHOG000218481.
KOK03040.
OMAKAESIHY.
OrthoDBEOG68WR84.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-499-MONOMER.

Family and domain databases

Gene3D2.170.120.12. 1 hit.
HAMAPMF_00059. RNApol_bact_RpoA.
InterProIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsTIGR02027. rpoA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRPOA_BUCAI
AccessionPrimary (citable) accession number: P57566
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names