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P57566

- RPOA_BUCAI

UniProt

P57566 - RPOA_BUCAI

Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP
    2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Transcription

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-499-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
    Short name:
    RNAP subunit alphaUniRule annotation
    Alternative name(s):
    RNA polymerase subunit alphaUniRule annotation
    Transcriptase subunit alphaUniRule annotation
    Gene namesi
    Name:rpoAUniRule annotation
    Ordered Locus Names:BU499
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Keywords - Cellular componenti

    DNA-directed RNA polymerase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329DNA-directed RNA polymerase subunit alphaPRO_0000175279Add
    BLAST

    Proteomic databases

    PRIDEiP57566.

    Interactioni

    Subunit structurei

    Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57566.
    SMRiP57566. Positions 1-235, 249-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 235235Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd
    BLAST
    Regioni249 – 32981Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd
    BLAST

    Domaini

    The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

    Sequence similaritiesi

    Belongs to the RNA polymerase alpha chain family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0202.
    HOGENOMiHOG000218481.
    KOiK03040.
    OMAiKAESIHY.
    OrthoDBiEOG68WR84.

    Family and domain databases

    Gene3Di2.170.120.12. 1 hit.
    HAMAPiMF_00059. RNApol_bact_RpoA.
    InterProiIPR011262. DNA-dir_RNA_pol_insert.
    IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
    IPR011773. DNA-dir_RpoA.
    IPR009025. RBP11-like_dimer.
    IPR011260. RNAP_asu_C.
    [Graphical view]
    PfamiPF01000. RNA_pol_A_bac. 1 hit.
    PF03118. RNA_pol_A_CTD. 1 hit.
    PF01193. RNA_pol_L. 1 hit.
    [Graphical view]
    ProDomiPD001179. RNAP_asu_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00662. RPOLD. 1 hit.
    [Graphical view]
    SUPFAMiSSF55257. SSF55257. 2 hits.
    SSF56553. SSF56553. 1 hit.
    TIGRFAMsiTIGR02027. rpoA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P57566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNSIMDFLR PRLVDIEQIS ATHTKVTLEP LERGFGHTLG NALRRILLSS    50
    MPGCAVTEVE IDGVLHEYST KEGVQEDVLE ILLNLKALAV KVYGKDETLL 100
    TLHKSGIGSI TAADIIHDGD VEIIKPQHVI CHLTDENASI KMRIKVQRGR 150
    GYIPASSRVH LEEDSRPIGC LLVDACYSPI NRISYNVEAA RVEQRTDLDK 200
    LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDIREP EFKEEKPEFE 250
    PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL 300
    TEIKDVLASR SLSLGMRLEN WPPSSILDE 329
    Length:329
    Mass (Da):36,855
    Last modified:December 1, 2000 - v1
    Checksum:i8340F07893FF0D85
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13192.1.
    RefSeqiNP_240306.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13192; BAB13192; BAB13192.
    GeneIDi1109842.
    KEGGibuc:BU499.
    PATRICi21244540. VBIBucAph127364_0504.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13192.1 .
    RefSeqi NP_240306.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57566.
    SMRi P57566. Positions 1-235, 249-329.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P57566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13192 ; BAB13192 ; BAB13192 .
    GeneIDi 1109842.
    KEGGi buc:BU499.
    PATRICi 21244540. VBIBucAph127364_0504.

    Phylogenomic databases

    eggNOGi COG0202.
    HOGENOMi HOG000218481.
    KOi K03040.
    OMAi KAESIHY.
    OrthoDBi EOG68WR84.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-499-MONOMER.

    Family and domain databases

    Gene3Di 2.170.120.12. 1 hit.
    HAMAPi MF_00059. RNApol_bact_RpoA.
    InterProi IPR011262. DNA-dir_RNA_pol_insert.
    IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
    IPR011773. DNA-dir_RpoA.
    IPR009025. RBP11-like_dimer.
    IPR011260. RNAP_asu_C.
    [Graphical view ]
    Pfami PF01000. RNA_pol_A_bac. 1 hit.
    PF03118. RNA_pol_A_CTD. 1 hit.
    PF01193. RNA_pol_L. 1 hit.
    [Graphical view ]
    ProDomi PD001179. RNAP_asu_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00662. RPOLD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55257. SSF55257. 2 hits.
    SSF56553. SSF56553. 1 hit.
    TIGRFAMsi TIGR02027. rpoA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiRPOA_BUCAI
    AccessioniPrimary (citable) accession number: P57566
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3