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P57566

- RPOA_BUCAI

UniProt

P57566 - RPOA_BUCAI

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Protein
DNA-directed RNA polymerase subunit alpha
Gene
rpoA, BU499
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-HAMAP
  2. DNA-directed RNA polymerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-499-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alpha (EC:2.7.7.6)
Short name:
RNAP subunit alpha
Alternative name(s):
RNA polymerase subunit alpha
Transcriptase subunit alpha
Gene namesi
Name:rpoA
Ordered Locus Names:BU499
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329DNA-directed RNA polymerase subunit alphaUniRule annotation
PRO_0000175279Add
BLAST

Proteomic databases

PRIDEiP57566.

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57566.
SMRiP57566. Positions 1-235, 249-329.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 235235Alpha N-terminal domain (alpha-NTD) By similarity
Add
BLAST
Regioni249 – 32981Alpha C-terminal domain (alpha-CTD) By similarity
Add
BLAST

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0202.
HOGENOMiHOG000218481.
KOiK03040.
OMAiKAESIHY.
OrthoDBiEOG68WR84.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
HAMAPiMF_00059. RNApol_bact_RpoA.
InterProiIPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
ProDomiPD001179. RNAP_asu_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsiTIGR02027. rpoA. 1 hit.

Sequencei

Sequence statusi: Complete.

P57566-1 [UniParc]FASTAAdd to Basket

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MQNSIMDFLR PRLVDIEQIS ATHTKVTLEP LERGFGHTLG NALRRILLSS    50
MPGCAVTEVE IDGVLHEYST KEGVQEDVLE ILLNLKALAV KVYGKDETLL 100
TLHKSGIGSI TAADIIHDGD VEIIKPQHVI CHLTDENASI KMRIKVQRGR 150
GYIPASSRVH LEEDSRPIGC LLVDACYSPI NRISYNVEAA RVEQRTDLDK 200
LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDIREP EFKEEKPEFE 250
PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL 300
TEIKDVLASR SLSLGMRLEN WPPSSILDE 329
Length:329
Mass (Da):36,855
Last modified:December 1, 2000 - v1
Checksum:i8340F07893FF0D85
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13192.1.
RefSeqiNP_240306.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13192; BAB13192; BAB13192.
GeneIDi1109842.
KEGGibuc:BU499.
PATRICi21244540. VBIBucAph127364_0504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13192.1 .
RefSeqi NP_240306.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57566.
SMRi P57566. Positions 1-235, 249-329.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P57566.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13192 ; BAB13192 ; BAB13192 .
GeneIDi 1109842.
KEGGi buc:BU499.
PATRICi 21244540. VBIBucAph127364_0504.

Phylogenomic databases

eggNOGi COG0202.
HOGENOMi HOG000218481.
KOi K03040.
OMAi KAESIHY.
OrthoDBi EOG68WR84.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-499-MONOMER.

Family and domain databases

Gene3Di 2.170.120.12. 1 hit.
HAMAPi MF_00059. RNApol_bact_RpoA.
InterProi IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR011773. DNA-dir_RpoA.
IPR009025. RBP11-like_dimer.
IPR011260. RNAP_asu_C.
[Graphical view ]
Pfami PF01000. RNA_pol_A_bac. 1 hit.
PF03118. RNA_pol_A_CTD. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view ]
ProDomi PD001179. RNAP_asu_C. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00662. RPOLD. 1 hit.
[Graphical view ]
SUPFAMi SSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
TIGRFAMsi TIGR02027. rpoA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiRPOA_BUCAI
AccessioniPrimary (citable) accession number: P57566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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