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P57558

- SYC_BUCAI

UniProt

P57558 - SYC_BUCAI

Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi28 – 281ZincBy similarity
    Metal bindingi209 – 2091ZincBy similarity
    Metal bindingi234 – 2341ZincBy similarity
    Metal bindingi238 – 2381ZincBy similarity
    Binding sitei269 – 2691ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. cysteine-tRNA ligase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cysteinyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-487-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine--tRNA ligase (EC:6.1.1.16)
    Alternative name(s):
    Cysteinyl-tRNA synthetase
    Short name:
    CysRS
    Gene namesi
    Name:cysS
    Ordered Locus Names:BU487
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Cysteine--tRNA ligasePRO_0000159366Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57558.
    SMRiP57558. Positions 1-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi30 – 4011"HIGH" regionAdd
    BLAST
    Motifi266 – 2705"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0215.
    HOGENOMiHOG000245250.
    KOiK01883.
    OMAiMHADFDA.
    OrthoDBiEOG6RVFXC.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00041. Cys_tRNA_synth.
    InterProiIPR015803. Cys-tRNA-ligase.
    IPR015273. Cys-tRNA-synt_Ia_DALR.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PANTHERiPTHR10890. PTHR10890. 1 hit.
    PfamiPF09190. DALR_2. 1 hit.
    PF01406. tRNA-synt_1e. 1 hit.
    [Graphical view]
    PRINTSiPR00983. TRNASYNTHCYS.
    SMARTiSM00840. DALR_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    TIGRFAMsiTIGR00435. cysS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P57558-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKIFNTLTS TKEIFTPIKK NRVNLYVCGV TVYDFCHIGH GRTFVVFDMI    50
    VRYLRFSGFQ VKYVRNITDI DDKIISKSTK EKKKINTFTA SMIKEMHKDF 100
    DLLGISVPDE EPRVTDYIDN IIRIITTLIK KKHAYIHKNG DVIFSIDSDP 150
    NYGTLSRQSL TSLESGSRIP LNNMKKNPLD FILWKSSNKE EYSWDSPWGK 200
    GRPGWHIECS AITNVFFNNS IDIHGGGSDL LFPHHENERS QSICFNNKSM 250
    INFWMHTGMV ILNNKKMSKS LGNVYFLRNI LKDCDAEVLR YFFLSTHYRH 300
    PIYYCEKNLD QAYTSLKYLY TALYDTNPFF NNEEGLNFEL EFYNAMNDDF 350
    NTPAVFSIFF KIARKINFLK NKDILKTNKF AFRLKYLANN LGFLFQDPKE 400
    FLQKKTTLNL LTLKEIQLLI EKRNIARQSK LWQEADNIRK KLMSLDIILE 450
    DLPDKTIWRK NKKS 464
    Length:464
    Mass (Da):54,541
    Last modified:December 1, 2000 - v1
    Checksum:i2EBD5EADA503AAC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13183.1.
    RefSeqiNP_240297.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13183; BAB13183; BAB13183.
    GeneIDi1109833.
    KEGGibuc:BU487.
    PATRICi21244516. VBIBucAph127364_0495.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13183.1 .
    RefSeqi NP_240297.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57558.
    SMRi P57558. Positions 1-402.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13183 ; BAB13183 ; BAB13183 .
    GeneIDi 1109833.
    KEGGi buc:BU487.
    PATRICi 21244516. VBIBucAph127364_0495.

    Phylogenomic databases

    eggNOGi COG0215.
    HOGENOMi HOG000245250.
    KOi K01883.
    OMAi MHADFDA.
    OrthoDBi EOG6RVFXC.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-487-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00041. Cys_tRNA_synth.
    InterProi IPR015803. Cys-tRNA-ligase.
    IPR015273. Cys-tRNA-synt_Ia_DALR.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    PANTHERi PTHR10890. PTHR10890. 1 hit.
    Pfami PF09190. DALR_2. 1 hit.
    PF01406. tRNA-synt_1e. 1 hit.
    [Graphical view ]
    PRINTSi PR00983. TRNASYNTHCYS.
    SMARTi SM00840. DALR_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    TIGRFAMsi TIGR00435. cysS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiSYC_BUCAI
    AccessioniPrimary (citable) accession number: P57558
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3