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P57558

- SYC_BUCAI

UniProt

P57558 - SYC_BUCAI

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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi28 – 281ZincBy similarity
Metal bindingi209 – 2091ZincBy similarity
Metal bindingi234 – 2341ZincBy similarity
Metal bindingi238 – 2381ZincBy similarity
Binding sitei269 – 2691ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. cysteine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteinyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-487-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:BU487
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Cysteine--tRNA ligasePRO_0000159366Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP57558.
SMRiP57558. Positions 1-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi30 – 4011"HIGH" regionAdd
BLAST
Motifi266 – 2705"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiMHADFDA.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

P57558-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKIFNTLTS TKEIFTPIKK NRVNLYVCGV TVYDFCHIGH GRTFVVFDMI
60 70 80 90 100
VRYLRFSGFQ VKYVRNITDI DDKIISKSTK EKKKINTFTA SMIKEMHKDF
110 120 130 140 150
DLLGISVPDE EPRVTDYIDN IIRIITTLIK KKHAYIHKNG DVIFSIDSDP
160 170 180 190 200
NYGTLSRQSL TSLESGSRIP LNNMKKNPLD FILWKSSNKE EYSWDSPWGK
210 220 230 240 250
GRPGWHIECS AITNVFFNNS IDIHGGGSDL LFPHHENERS QSICFNNKSM
260 270 280 290 300
INFWMHTGMV ILNNKKMSKS LGNVYFLRNI LKDCDAEVLR YFFLSTHYRH
310 320 330 340 350
PIYYCEKNLD QAYTSLKYLY TALYDTNPFF NNEEGLNFEL EFYNAMNDDF
360 370 380 390 400
NTPAVFSIFF KIARKINFLK NKDILKTNKF AFRLKYLANN LGFLFQDPKE
410 420 430 440 450
FLQKKTTLNL LTLKEIQLLI EKRNIARQSK LWQEADNIRK KLMSLDIILE
460
DLPDKTIWRK NKKS
Length:464
Mass (Da):54,541
Last modified:December 1, 2000 - v1
Checksum:i2EBD5EADA503AAC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13183.1.
RefSeqiNP_240297.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13183; BAB13183; BAB13183.
GeneIDi1109833.
KEGGibuc:BU487.
PATRICi21244516. VBIBucAph127364_0495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13183.1 .
RefSeqi NP_240297.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57558.
SMRi P57558. Positions 1-402.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13183 ; BAB13183 ; BAB13183 .
GeneIDi 1109833.
KEGGi buc:BU487.
PATRICi 21244516. VBIBucAph127364_0495.

Phylogenomic databases

eggNOGi COG0215.
HOGENOMi HOG000245250.
KOi K01883.
OMAi MHADFDA.
OrthoDBi EOG6RVFXC.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-487-MONOMER.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
HAMAPi MF_00041. Cys_tRNA_synth.
InterProi IPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
PANTHERi PTHR10890. PTHR10890. 1 hit.
Pfami PF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view ]
PRINTSi PR00983. TRNASYNTHCYS.
SMARTi SM00840. DALR_2. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
TIGRFAMsi TIGR00435. cysS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiSYC_BUCAI
AccessioniPrimary (citable) accession number: P57558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 26, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3