P57558 (SYC_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	BU487

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000159366
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57558 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 2EBD5EADA503AAC3	FASTA	464	54,541
	10 20 30 40 50 60 MLKIFNTLTS TKEIFTPIKK NRVNLYVCGV TVYDFCHIGH GRTFVVFDMI VRYLRFSGFQ 70 80 90 100 110 120 VKYVRNITDI DDKIISKSTK EKKKINTFTA SMIKEMHKDF DLLGISVPDE EPRVTDYIDN 130 140 150 160 170 180 IIRIITTLIK KKHAYIHKNG DVIFSIDSDP NYGTLSRQSL TSLESGSRIP LNNMKKNPLD 190 200 210 220 230 240 FILWKSSNKE EYSWDSPWGK GRPGWHIECS AITNVFFNNS IDIHGGGSDL LFPHHENERS 250 260 270 280 290 300 QSICFNNKSM INFWMHTGMV ILNNKKMSKS LGNVYFLRNI LKDCDAEVLR YFFLSTHYRH 310 320 330 340 350 360 PIYYCEKNLD QAYTSLKYLY TALYDTNPFF NNEEGLNFEL EFYNAMNDDF NTPAVFSIFF 370 380 390 400 410 420 KIARKINFLK NKDILKTNKF AFRLKYLANN LGFLFQDPKE FLQKKTTLNL LTLKEIQLLI 430 440 450 460 EKRNIARQSK LWQEADNIRK KLMSLDIILE DLPDKTIWRK NKKS

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13183.1.
RefSeq	NP_240297.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57558.
SMR	P57558. Positions 1-402.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13183; BAB13183; BAB13183.
GeneID	1109833.
KEGG	buc:BU487.
PATRIC	21244516. VBIBucAph127364_0495.
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HOG000245250.
KO	K01883.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_BUCAI

Accession

Primary (citable) accession number: P57558

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program