P57552 (MDLB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multidrug resistance-like ATP-binding protein MdlB
EC=3.6.3.44

Gene names

Name:	mdlB
Ordered Locus Names:	BU480

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.
Sequence caution	The sequence BAB13177.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Multidrug resistance-like ATP-binding protein MdlB		PRO_0000092499
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Transmembrane	268 – 288	21	Helical; Potential
	Domain	25 – 310	286	ABC transmembrane type-1
	Domain	341 – 575	235	ABC transporter
	Nucleotide binding	375 – 382	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P57552 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: BA2872BA2E76E200	FASTA	580	66,403
	10 20 30 40 50 60 MNHLIAFWPI LKRLLIYVKP HKKKLILAFI FLLSGSTSEV LGPILISYFI NNILSKHQLH 70 80 90 100 110 120 LLIILTIITL FIILQILSVF LNYFQSILFN KIAVESINKL RQDVMYAALQ QPISEFDSQP 130 140 150 160 170 180 IGQMISKVTN DTEAVKELYD TVGPTLFRSI ILIFIILFAM FTLEWHMALV ALFILPLVIT 190 200 210 220 230 240 IMLVYQYYST PLLRKVRYYL AEINNKFNET INGMNVIQQF CQQRRFQEKI KKSSDLHYMS 250 260 270 280 290 300 RMKILRLDGF LLRPLLSLLS SMILCNFMFL FSFFPVGAFE VGVLYAFITY LGRLNEPLIA 310 320 330 340 350 360 ITIQQSVLQQ SIVAGERIFS LIDSPKQKYG KNKDLLKSGK INIQNVSFYH KNCNKNILEN 370 380 390 400 410 420 INIKISSKSF VAFVGHTGSG KSTLANLIMG YYPLKNGKIY LDDKSIDSIS HSVLRRNVLM 430 440 450 460 470 480 VQQDPIVLSD TFFYNITLGR KIPEEKVWNI LDTVHLSDLV KSMPKGIYSL LGEEGNNLSV 490 500 510 520 530 540 GQKQLLAIAR VLVAYPKVLI LDEATANIDS GTEQLIQKTL LSIRKNCTLI IIAHRLSTII 550 560 570 580 EADSIIVLKK GKIVEFGTHE QLLTKKSCYY KMYKFQSCKF

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13177.1. Different initiation.
RefSeq	NP_240291.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57552.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002958; EBBUCP00000002734; EBBUCG00000002958.
GeneID	1109827.
GenomeReviews	Gene locus BU480 in contig BA000003_GR.
KEGG	buc:BU480.
PATRIC	21244502. VBIBucAph127364_0489.
Phylogenomic databases
GeneTree	EBGT00050000007760.
HOGENOM	HBG758042.
OMA	MYQLQQV.
PhylomeDB	P57552.
ProtClustDB	CLSK341927.
Enzyme and pathway databases
BioCyc	BSP107806:BU480-MONOMER.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K06148.
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDLB_BUCAI

Accession

Primary (citable) accession number: P57552

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program