Reviewed, UniProtKB/Swiss-Prot P57551 (MDLA_BUCAI)

Names and origin

Protein names

Recommended name:
    Multidrug resistance-like ATP-binding protein mdlA
    EC=3.6.3.44

Gene names

Name:	mdlA
Ordered Locus Names:	BU479

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the ABC transporter superfamily. Drug exporter-2 (TC 3.A.1.117) family. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Multidrug resistance-like ATP-binding protein mdlA		PRO_0000092493
Regions
	Transmembrane	23 – 43	21	Potential
	Transmembrane	53 – 73	21	Potential
	Transmembrane	134 – 154	21	Potential
	Transmembrane	155 – 175	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	280 – 300	21	Potential
	Domain	18 – 303	286	ABC transmembrane type-1
	Domain	334 – 571	238	ABC transporter
	Nucleotide binding	369 – 376	8	ATP Potential
	Compositional bias	519 – 522	4	Poly-Lys

Sequences

	Sequence		Length	Mass (Da)
	P57551-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: AF736FA2FA60A56A	FASTA	589	67,522
	10 20 30 40 50 60 MKLFNQLRWF FVREWKRYLG AILLLIIIAT LQLLPPKIVG VLIDLIIKKN MHGVQILPWI 70 80 90 100 110 120 LIIFLVAVIV YILRYLWRIL LFGASYQLAT ELRVKFYTYL SQQSQIFFLK NRTGDLIARA 130 140 150 160 170 180 TNDVDRVVFA AGEGVLTLVD SLVMGFSVLI VMSTQISFLL TIISLIPMPI MAILIKKYGK 190 200 210 220 230 240 ELHETFRHAQ ISFSLLNNQT QEILTSIRMI RAFGLEKNQS NKFNIITRNT GKKNMEVAKI 250 260 270 280 290 300 DARFDPVIYL SVAFSNLLAI IGGGWLVWNN QISIGQLTSF IMYLGLMIWP MLALAWMFNI 310 320 330 340 350 360 VERGSAAWDR IHSIINKKLY IEDGKNNIPP YPGILNINIK KFYYPNNQKP SLKDIRILIK 370 380 390 400 410 420 PGNTLGICGP TGSGKSTLLM LIQRQFNFKK EEIHYHSLSL LEIKIDDWRR RIAVVNQTSF 430 440 450 460 470 480 LFSDTISNNI SLGKPNASQK EIEEVAKLAD VHKDIIDLPE RYETQVGERG VMLSGGQKQR 490 500 510 520 530 540 VCIARALLLN AEILILDDAL SAVDAQTENN ILKNINEWKK KKHSLIITTH RLSALINSDE 550 560 570 580 IIVIKSGSII QRGNHLKLIQ EKNWYKSMYY YQQSEIELED NEKTGEKNL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13176.1.
RefSeq	NP_240290.1.
3D structure databases
SMR	P57551. Positions 10-573.
ModBase	Search...
Genome annotation databases
GeneID	1109826.
GenomeReviews	Gene locus BU479 in contig BA000003_GR.
KEGG	buc:BU479.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758042.
OMA	WNNTITI.
PhylomeDB	P57551.
Enzyme and pathway databases
BioCyc	BSP107806:BU479-MONOMER.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDLA_BUCAI

Accession

Primary (citable) accession number: P57551

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 19, 2010
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)