P57549 (LON_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease
EC=3.4.21.53

Alternative name(s):
ATP-dependent protease La

Gene names

Name:	lon
Ordered Locus Names:	BU477

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homohexamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Cytoplasm.
Induction	By heat shock By similarity.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	Lon protease		PRO_0000076125
Regions
	Domain	10 – 201	192	Lon
	Nucleotide binding	356 – 363	8	ATP By similarity
Sites
	Active site	679	1	By similarity
	Active site	722	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57549 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 729E606CBB86A3E7	FASTA	777	88,033
	10 20 30 40 50 60 MNSERSERIT IPVLPLRDVV IYPHMVIPLF VGRQKSIKCI ETSMSNDKKI MLIAQKEASK 70 80 90 100 110 120 DEPTPKDLFD IGTISAILQM LKLPDGTVKV LIEGLQRAHI KNLTNNGEHF IAEVELISSS 130 140 150 160 170 180 NLLDKNQEVL IRTTMNQFES YIKLNKKIPL EILNVLNNIK NSEKLADTIA AHMPLKLNDK 190 200 210 220 230 240 QSVLEIRNIN DRLEFLMAIM ESEIDLLQVE KRIRHRVKKQ MEKSQREYYL NEQIKAIQKE 250 260 270 280 290 300 LGDMDEIPDE NKILKRKIKS SKMPKEAREK TELELQKLKM MSPMSAEATV VRSYIDWMIQ 310 320 330 340 350 360 VPWYLKTKIK KDLQQAKKIL DIDHFGLETV KDRILEYLAV QSRKNKIKGP ILCLIGPPGV 370 380 390 400 410 420 GKTSLGKSIA RSTGRKYVRI ALGGIRDEAE IRGHRRTYIG SMPGKLIQKM AKAKVKNPLF 430 440 450 460 470 480 LLDEIDKMSR DIRVDPASAL LEVLDSEQNM NFNDHYLEVD YDLSDVMFVA TSNSMNIPAP 490 500 510 520 530 540 LLDRMEIIRL SGYTEDEKLN IAKRYLYPKQ IERNGLEENE IKITDSAIIS IIHYYTREAG 550 560 570 580 590 600 VRSLEREISK ICRKAVKNLI LDKSLKHIEI NSKNLKKFLG IKRFDYGKIH GTNQIGQVIG 610 620 630 640 650 660 LAWTEVGGEL LTIETTCVSG KGKLTYTGSL GEVMQESIQA ALTVVRSQAD RLGIKKDFHE 670 680 690 700 710 720 KHDIHVHVPE GATPKDGPSA GAAMCTAIVS SLTNNPVKSN VAMTGEITLH GKILPIGGLK 730 740 750 760 770 EKLLAAHRGG IKTVLIPYEN KRNLEEIPKN IIEGLNIHPI KKIEEVLKLS LEKIPYV

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13174.1.
RefSeq	NP_240288.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57549.
SMR	P57549. Positions 8-117, 491-584, 593-772.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003204; EBBUCP00000002980; EBBUCG00000003204.
GeneID	1109824.
GenomeReviews	Gene locus BU477 in contig BA000003_GR.
KEGG	buc:BU477.
PATRIC	21244496. VBIBucAph127364_0486.
Phylogenomic databases
GeneTree	EBGT00050000007957.
HOGENOM	HBG566281.
OMA	PDENERD.
PhylomeDB	P57549.
ProtClustDB	CLSK316282.
Enzyme and pathway databases
BioCyc	BSP107806:BU477-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
KO	K01338.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. Lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_BUCAI

Accession

Primary (citable) accession number: P57549

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program