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Protein

Cytochrome bo(3) ubiquinol oxidase subunit 1

Gene

cyoB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron (By similarity).By similarity

Catalytic activityi

2 ubiquinol + O2 + n H+(Side 1) = 2 ubiquinone + 2 H2O + n H+(Side 2).

Cofactori

Protein has several cofactor binding sites:
  • Cu2+By similarityNote: Binds 1 copper B ion per subunit.By similarity
  • heme bBy similarityNote: Binds 1 low-spin heme b per subunit.By similarity
  • heme oBy similarityNote: Binds 1 high-spin heme o per subunit.By similarity
  • a quinoneBy similarityNote: Binds 1 high-affinity quinone that appears to function as a tightly bound cofactor (QH), forming a semiquinone intermediate in the reaction.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71Quinone (QH)By similarity1
Binding sitei75Quinone (QH)By similarity1
Binding sitei98Quinone (QH)By similarity1
Binding sitei101Quinone (QH)By similarity1
Metal bindingi106Iron (heme b axial ligand)By similarity1
Metal bindingi284CopperBy similarity1
Metal bindingi288CopperBy similarity1
Metal bindingi333CopperBy similarity1
Metal bindingi334CopperBy similarity1
Metal bindingi419Iron (heme o axial ligand)By similarity1
Metal bindingi421Iron (heme b axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Hydrogen ion transport, Ion transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bo(3) ubiquinol oxidase subunit 1 (EC:1.10.3.10)
Alternative name(s):
Cytochrome o ubiquinol oxidase subunit 1
Short name:
Cytochrome o subunit 1
Oxidase bo(3) subunit 1
Ubiquinol oxidase polypeptide I
Ubiquinol oxidase subunit 1
Gene namesi
Name:cyoB
Ordered Locus Names:BU471
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14ExtracellularSequence analysisAdd BLAST14
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Topological domaini36 – 58CytoplasmicSequence analysisAdd BLAST23
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
Topological domaini80 – 106ExtracellularSequence analysisAdd BLAST27
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128 – 145CytoplasmicSequence analysisAdd BLAST18
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Topological domaini167 – 189ExtracellularSequence analysisAdd BLAST23
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 232CytoplasmicSequence analysisAdd BLAST22
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Topological domaini254 – 277ExtracellularSequence analysisAdd BLAST24
Transmembranei278 – 298HelicalSequence analysisAdd BLAST21
Topological domaini299 – 309CytoplasmicSequence analysisAdd BLAST11
Transmembranei310 – 330HelicalSequence analysisAdd BLAST21
Topological domaini331 – 346ExtracellularSequence analysisAdd BLAST16
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21
Topological domaini368 – 380CytoplasmicSequence analysisAdd BLAST13
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Topological domaini402 – 413ExtracellularSequence analysisAdd BLAST12
Transmembranei414 – 434HelicalSequence analysisAdd BLAST21
Topological domaini435 – 456CytoplasmicSequence analysisAdd BLAST22
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21
Topological domaini478 – 493ExtracellularSequence analysisAdd BLAST16
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 586CytoplasmicSequence analysisAdd BLAST72
Transmembranei587 – 607HelicalSequence analysisAdd BLAST21
Topological domaini608ExtracellularSequence analysis1
Transmembranei609 – 629HelicalSequence analysisAdd BLAST21
Topological domaini630 – 662CytoplasmicSequence analysisAdd BLAST33

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001834791 – 662Cytochrome bo(3) ubiquinol oxidase subunit 1Add BLAST662

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki284 ↔ 2881'-histidyl-3'-tyrosine (His-Tyr)By similarity

Interactioni

Subunit structurei

Heterooctamer of two A chains, two B chains, two C chains and two D chains.By similarity

Protein-protein interaction databases

STRINGi107806.BU471.

Structurei

3D structure databases

ProteinModelPortaliP57543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ9. Bacteria.
COG0843. LUCA.
HOGENOMiHOG000085275.
KOiK02298.
OMAiFCGKRLF.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR014207. Cyt_c_ubiqinol_oxidase_su1.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02843. CyoB. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGKLTFDAI PYHEPIIMIT YIAIILIALC IASTITYYKK WKYLWYEWFT
60 70 80 90 100
TVDHKKISIM YGILAFVMLF RGFVDAILMR TQQVVASAGF KGFLPPHHYD
110 120 130 140 150
QIFTAHGVIM IFFVAMPLVI GLMNLVIPLQ IGARDVAFPF LNNLSFWLNV
160 170 180 190 200
SSAVLLTLSL GIGEFAQTGW LAYPPLSGIK YSSGVGVDYW IWSLQISGVG
210 220 230 240 250
TTLTGINFLV TILKMRAPGM SFFKMPVFTW TSLCTNILIV ISFPVLTVTL
260 270 280 290 300
VLLTLDRYFN FHFFTNDLGG NAMMYVNLIW IWGHPEVYIL VLPVFGVFSE
310 320 330 340 350
VVATFSKKRL FGYVSLVWAT LSITILSFIV WLHHFFTMGA GADVNTFFGI
360 370 380 390 400
TTMIIAIPTG VKIFNWLFTI YQGRVHMHSS ILWTLGFLVT FSIGGMTGVL
410 420 430 440 450
LSVPPADFVL HNSLFLVAHF HNVIIGGVVF GCFAGINYWF PKLFGFVLNE
460 470 480 490 500
IWGKRAFWFW IIGFFLAFIP LYFLGLMGMT RRLSQNIDSE FHMLLCIAAI
510 520 530 540 550
GACFIGIGII CQVIQFFISI KERRHNLDLT GDPWDGRTLE WSTSSPAPLY
560 570 580 590 600
NFAIIPKVED RDDFWRNKEG QHYNKLINSI NYHDIHMPKN TGLGFMISIF
610 620 630 640 650
SLFFGFSAVW HITWLCILSF LAIIISLFIN SLNEDTEYTI SAEEIKKIEH
660
QYWKNIQKAG LK
Length:662
Mass (Da):75,455
Last modified:December 1, 2000 - v1
Checksum:i3F502A628133EA65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13168.1.
RefSeqiNP_240282.1. NC_002528.1.
WP_010896132.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13168; BAB13168; BAB13168.
GeneIDi1109818.
KEGGibuc:BU471.
PATRICi21244484. VBIBucAph127364_0480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13168.1.
RefSeqiNP_240282.1. NC_002528.1.
WP_010896132.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU471.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13168; BAB13168; BAB13168.
GeneIDi1109818.
KEGGibuc:BU471.
PATRICi21244484. VBIBucAph127364_0480.

Phylogenomic databases

eggNOGiENOG4105BZ9. Bacteria.
COG0843. LUCA.
HOGENOMiHOG000085275.
KOiK02298.
OMAiFCGKRLF.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR014207. Cyt_c_ubiqinol_oxidase_su1.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02843. CyoB. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYOB_BUCAI
AccessioniPrimary (citable) accession number: P57543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Ubiquinol oxidase catalyzes the terminal step in the electron transport chain.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.