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P57543

- CYOB_BUCAI

UniProt

P57543 - CYOB_BUCAI

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Protein
Cytochrome bo(3) ubiquinol oxidase subunit 1
Gene
cyoB, BU471
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron By similarity.

Catalytic activityi

2 ubiquinol + O2 + n H+(Side 1) = 2 ubiquinone + 2 H2O + n H+(Side 2).

Cofactori

Binds 1 copper B ion per subunit By similarity.
Binds 1 low-spin heme b per subunit By similarity.
Binds 1 high-spin heme o per subunit By similarity.
Bind 1 high-affinity quinone that appears to function as a tightly bound cofactor (QH), forming a semiquinone intermediate in the reaction By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711Quinone (QH) By similarity
Binding sitei75 – 751Quinone (QH) By similarity
Binding sitei98 – 981Quinone (QH) By similarity
Binding sitei101 – 1011Quinone (QH) By similarity
Metal bindingi106 – 1061Iron (heme b axial ligand) By similarity
Metal bindingi284 – 2841Copper By similarity
Metal bindingi288 – 2881Copper By similarity
Metal bindingi333 – 3331Copper By similarity
Metal bindingi334 – 3341Copper By similarity
Metal bindingi419 – 4191Iron (heme o axial ligand) By similarity
Metal bindingi421 – 4211Iron (heme b axial ligand) By similarity

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: InterPro
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Hydrogen ion transport, Ion transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-471-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bo(3) ubiquinol oxidase subunit 1 (EC:1.10.3.10)
Alternative name(s):
Cytochrome o ubiquinol oxidase subunit 1
Short name:
Cytochrome o subunit 1
Oxidase bo(3) subunit 1
Ubiquinol oxidase polypeptide I
Ubiquinol oxidase subunit 1
Gene namesi
Name:cyoB
Ordered Locus Names:BU471
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414Extracellular Reviewed prediction
Add
BLAST
Transmembranei15 – 3521Helical; Reviewed prediction
Add
BLAST
Topological domaini36 – 5823Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei59 – 7921Helical; Reviewed prediction
Add
BLAST
Topological domaini80 – 10627Extracellular Reviewed prediction
Add
BLAST
Transmembranei107 – 12721Helical; Reviewed prediction
Add
BLAST
Topological domaini128 – 14518Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei146 – 16621Helical; Reviewed prediction
Add
BLAST
Topological domaini167 – 18923Extracellular Reviewed prediction
Add
BLAST
Transmembranei190 – 21021Helical; Reviewed prediction
Add
BLAST
Topological domaini211 – 23222Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei233 – 25321Helical; Reviewed prediction
Add
BLAST
Topological domaini254 – 27724Extracellular Reviewed prediction
Add
BLAST
Transmembranei278 – 29821Helical; Reviewed prediction
Add
BLAST
Topological domaini299 – 30911Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei310 – 33021Helical; Reviewed prediction
Add
BLAST
Topological domaini331 – 34616Extracellular Reviewed prediction
Add
BLAST
Transmembranei347 – 36721Helical; Reviewed prediction
Add
BLAST
Topological domaini368 – 38013Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei381 – 40121Helical; Reviewed prediction
Add
BLAST
Topological domaini402 – 41312Extracellular Reviewed prediction
Add
BLAST
Transmembranei414 – 43421Helical; Reviewed prediction
Add
BLAST
Topological domaini435 – 45622Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei457 – 47721Helical; Reviewed prediction
Add
BLAST
Topological domaini478 – 49316Extracellular Reviewed prediction
Add
BLAST
Transmembranei494 – 51421Helical; Reviewed prediction
Add
BLAST
Topological domaini515 – 58672Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei587 – 60721Helical; Reviewed prediction
Add
BLAST
Topological domaini608 – 6081Extracellular Reviewed prediction
Transmembranei609 – 62921Helical; Reviewed prediction
Add
BLAST
Topological domaini630 – 66233Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
  3. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Cytochrome bo(3) ubiquinol oxidase subunit 1
PRO_0000183479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki284 ↔ 2881'-histidyl-3'-tyrosine (His-Tyr) By similarity

Interactioni

Subunit structurei

Heterooctamer of two A chains, two B chains, two C chains and two D chains By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57543.
SMRiP57543. Positions 52-552.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0843.
HOGENOMiHOG000085275.
KOiK02298.
OMAiMGMTRRV.
OrthoDBiEOG6B35XR.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR014207. Cyt_c_ubiqinol_oxidase_su1.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02843. CyoB. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57543-1 [UniParc]FASTAAdd to Basket

« Hide

MFGKLTFDAI PYHEPIIMIT YIAIILIALC IASTITYYKK WKYLWYEWFT    50
TVDHKKISIM YGILAFVMLF RGFVDAILMR TQQVVASAGF KGFLPPHHYD 100
QIFTAHGVIM IFFVAMPLVI GLMNLVIPLQ IGARDVAFPF LNNLSFWLNV 150
SSAVLLTLSL GIGEFAQTGW LAYPPLSGIK YSSGVGVDYW IWSLQISGVG 200
TTLTGINFLV TILKMRAPGM SFFKMPVFTW TSLCTNILIV ISFPVLTVTL 250
VLLTLDRYFN FHFFTNDLGG NAMMYVNLIW IWGHPEVYIL VLPVFGVFSE 300
VVATFSKKRL FGYVSLVWAT LSITILSFIV WLHHFFTMGA GADVNTFFGI 350
TTMIIAIPTG VKIFNWLFTI YQGRVHMHSS ILWTLGFLVT FSIGGMTGVL 400
LSVPPADFVL HNSLFLVAHF HNVIIGGVVF GCFAGINYWF PKLFGFVLNE 450
IWGKRAFWFW IIGFFLAFIP LYFLGLMGMT RRLSQNIDSE FHMLLCIAAI 500
GACFIGIGII CQVIQFFISI KERRHNLDLT GDPWDGRTLE WSTSSPAPLY 550
NFAIIPKVED RDDFWRNKEG QHYNKLINSI NYHDIHMPKN TGLGFMISIF 600
SLFFGFSAVW HITWLCILSF LAIIISLFIN SLNEDTEYTI SAEEIKKIEH 650
QYWKNIQKAG LK 662
Length:662
Mass (Da):75,455
Last modified:December 1, 2000 - v1
Checksum:i3F502A628133EA65
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13168.1.
RefSeqiNP_240282.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13168; BAB13168; BAB13168.
GeneIDi1109818.
KEGGibuc:BU471.
PATRICi21244484. VBIBucAph127364_0480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13168.1 .
RefSeqi NP_240282.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57543.
SMRi P57543. Positions 52-552.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13168 ; BAB13168 ; BAB13168 .
GeneIDi 1109818.
KEGGi buc:BU471.
PATRICi 21244484. VBIBucAph127364_0480.

Phylogenomic databases

eggNOGi COG0843.
HOGENOMi HOG000085275.
KOi K02298.
OMAi MGMTRRV.
OrthoDBi EOG6B35XR.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-471-MONOMER.

Family and domain databases

Gene3Di 1.20.210.10. 1 hit.
InterProi IPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR014207. Cyt_c_ubiqinol_oxidase_su1.
[Graphical view ]
PANTHERi PTHR10422. PTHR10422. 1 hit.
Pfami PF00115. COX1. 1 hit.
[Graphical view ]
PRINTSi PR01165. CYCOXIDASEI.
SUPFAMi SSF81442. SSF81442. 1 hit.
TIGRFAMsi TIGR02843. CyoB. 1 hit.
PROSITEi PS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiCYOB_BUCAI
AccessioniPrimary (citable) accession number: P57543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Ubiquinol oxidase catalyzes the terminal step in the electron transport chain.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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