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P57543

- CYOB_BUCAI

UniProt

P57543 - CYOB_BUCAI

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Protein

Cytochrome bo(3) ubiquinol oxidase subunit 1

Gene

cyoB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron (By similarity).By similarity

Catalytic activityi

2 ubiquinol + O2 + n H+(Side 1) = 2 ubiquinone + 2 H2O + n H+(Side 2).

Cofactori

Protein has several cofactor binding sites:
  • Cu2+By similarityNote: Binds 1 copper B ion per subunit.By similarity
  • heme bBy similarityNote: Binds 1 low-spin heme b per subunit.By similarity
  • heme oBy similarityNote: Binds 1 high-spin heme o per subunit.By similarity
  • a quinoneBy similarityNote: Binds 1 high-affinity quinone that appears to function as a tightly bound cofactor (QH), forming a semiquinone intermediate in the reaction.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711Quinone (QH)By similarity
Binding sitei75 – 751Quinone (QH)By similarity
Binding sitei98 – 981Quinone (QH)By similarity
Binding sitei101 – 1011Quinone (QH)By similarity
Metal bindingi106 – 1061Iron (heme b axial ligand)By similarity
Metal bindingi284 – 2841CopperBy similarity
Metal bindingi288 – 2881CopperBy similarity
Metal bindingi333 – 3331CopperBy similarity
Metal bindingi334 – 3341CopperBy similarity
Metal bindingi419 – 4191Iron (heme o axial ligand)By similarity
Metal bindingi421 – 4211Iron (heme b axial ligand)By similarity

GO - Molecular functioni

  1. cytochrome-c oxidase activity Source: InterPro
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Hydrogen ion transport, Ion transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-471-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bo(3) ubiquinol oxidase subunit 1 (EC:1.10.3.10)
Alternative name(s):
Cytochrome o ubiquinol oxidase subunit 1
Short name:
Cytochrome o subunit 1
Oxidase bo(3) subunit 1
Ubiquinol oxidase polypeptide I
Ubiquinol oxidase subunit 1
Gene namesi
Name:cyoB
Ordered Locus Names:BU471
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414ExtracellularSequence AnalysisAdd
BLAST
Transmembranei15 – 3521HelicalSequence AnalysisAdd
BLAST
Topological domaini36 – 5823CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei59 – 7921HelicalSequence AnalysisAdd
BLAST
Topological domaini80 – 10627ExtracellularSequence AnalysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence AnalysisAdd
BLAST
Topological domaini128 – 14518CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence AnalysisAdd
BLAST
Topological domaini167 – 18923ExtracellularSequence AnalysisAdd
BLAST
Transmembranei190 – 21021HelicalSequence AnalysisAdd
BLAST
Topological domaini211 – 23222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei233 – 25321HelicalSequence AnalysisAdd
BLAST
Topological domaini254 – 27724ExtracellularSequence AnalysisAdd
BLAST
Transmembranei278 – 29821HelicalSequence AnalysisAdd
BLAST
Topological domaini299 – 30911CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence AnalysisAdd
BLAST
Topological domaini331 – 34616ExtracellularSequence AnalysisAdd
BLAST
Transmembranei347 – 36721HelicalSequence AnalysisAdd
BLAST
Topological domaini368 – 38013CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei381 – 40121HelicalSequence AnalysisAdd
BLAST
Topological domaini402 – 41312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei414 – 43421HelicalSequence AnalysisAdd
BLAST
Topological domaini435 – 45622CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence AnalysisAdd
BLAST
Topological domaini478 – 49316ExtracellularSequence AnalysisAdd
BLAST
Transmembranei494 – 51421HelicalSequence AnalysisAdd
BLAST
Topological domaini515 – 58672CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei587 – 60721HelicalSequence AnalysisAdd
BLAST
Topological domaini608 – 6081ExtracellularSequence Analysis
Transmembranei609 – 62921HelicalSequence AnalysisAdd
BLAST
Topological domaini630 – 66233CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
  3. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Cytochrome bo(3) ubiquinol oxidase subunit 1PRO_0000183479Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki284 ↔ 2881'-histidyl-3'-tyrosine (His-Tyr)By similarity

Interactioni

Subunit structurei

Heterooctamer of two A chains, two B chains, two C chains and two D chains.By similarity

Structurei

3D structure databases

ProteinModelPortaliP57543.
SMRiP57543. Positions 52-552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0843.
HOGENOMiHOG000085275.
KOiK02298.
OMAiMGMTRRV.
OrthoDBiEOG6B35XR.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR014207. Cyt_c_ubiqinol_oxidase_su1.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02843. CyoB. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57543-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFGKLTFDAI PYHEPIIMIT YIAIILIALC IASTITYYKK WKYLWYEWFT
60 70 80 90 100
TVDHKKISIM YGILAFVMLF RGFVDAILMR TQQVVASAGF KGFLPPHHYD
110 120 130 140 150
QIFTAHGVIM IFFVAMPLVI GLMNLVIPLQ IGARDVAFPF LNNLSFWLNV
160 170 180 190 200
SSAVLLTLSL GIGEFAQTGW LAYPPLSGIK YSSGVGVDYW IWSLQISGVG
210 220 230 240 250
TTLTGINFLV TILKMRAPGM SFFKMPVFTW TSLCTNILIV ISFPVLTVTL
260 270 280 290 300
VLLTLDRYFN FHFFTNDLGG NAMMYVNLIW IWGHPEVYIL VLPVFGVFSE
310 320 330 340 350
VVATFSKKRL FGYVSLVWAT LSITILSFIV WLHHFFTMGA GADVNTFFGI
360 370 380 390 400
TTMIIAIPTG VKIFNWLFTI YQGRVHMHSS ILWTLGFLVT FSIGGMTGVL
410 420 430 440 450
LSVPPADFVL HNSLFLVAHF HNVIIGGVVF GCFAGINYWF PKLFGFVLNE
460 470 480 490 500
IWGKRAFWFW IIGFFLAFIP LYFLGLMGMT RRLSQNIDSE FHMLLCIAAI
510 520 530 540 550
GACFIGIGII CQVIQFFISI KERRHNLDLT GDPWDGRTLE WSTSSPAPLY
560 570 580 590 600
NFAIIPKVED RDDFWRNKEG QHYNKLINSI NYHDIHMPKN TGLGFMISIF
610 620 630 640 650
SLFFGFSAVW HITWLCILSF LAIIISLFIN SLNEDTEYTI SAEEIKKIEH
660
QYWKNIQKAG LK
Length:662
Mass (Da):75,455
Last modified:December 1, 2000 - v1
Checksum:i3F502A628133EA65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13168.1.
RefSeqiNP_240282.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13168; BAB13168; BAB13168.
GeneIDi1109818.
KEGGibuc:BU471.
PATRICi21244484. VBIBucAph127364_0480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13168.1 .
RefSeqi NP_240282.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57543.
SMRi P57543. Positions 52-552.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13168 ; BAB13168 ; BAB13168 .
GeneIDi 1109818.
KEGGi buc:BU471.
PATRICi 21244484. VBIBucAph127364_0480.

Phylogenomic databases

eggNOGi COG0843.
HOGENOMi HOG000085275.
KOi K02298.
OMAi MGMTRRV.
OrthoDBi EOG6B35XR.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-471-MONOMER.

Family and domain databases

Gene3Di 1.20.210.10. 1 hit.
InterProi IPR000883. COX1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR014207. Cyt_c_ubiqinol_oxidase_su1.
[Graphical view ]
PANTHERi PTHR10422. PTHR10422. 1 hit.
Pfami PF00115. COX1. 1 hit.
[Graphical view ]
PRINTSi PR01165. CYCOXIDASEI.
SUPFAMi SSF81442. SSF81442. 1 hit.
TIGRFAMsi TIGR02843. CyoB. 1 hit.
PROSITEi PS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiCYOB_BUCAI
AccessioniPrimary (citable) accession number: P57543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 26, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Ubiquinol oxidase catalyzes the terminal step in the electron transport chain.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3