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P57543

- CYOB_BUCAI

UniProt

P57543 - CYOB_BUCAI

Protein

Cytochrome bo(3) ubiquinol oxidase subunit 1

Gene

cyoB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Cytochrome bo3 ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron By similarity.By similarity

    Catalytic activityi

    2 ubiquinol + O2 + n H+(Side 1) = 2 ubiquinone + 2 H2O + n H+(Side 2).

    Cofactori

    Binds 1 copper B ion per subunit.By similarity
    Binds 1 low-spin heme b per subunit.By similarity
    Binds 1 high-spin heme o per subunit.By similarity
    Bind 1 high-affinity quinone that appears to function as a tightly bound cofactor (QH), forming a semiquinone intermediate in the reaction.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei71 – 711Quinone (QH)By similarity
    Binding sitei75 – 751Quinone (QH)By similarity
    Binding sitei98 – 981Quinone (QH)By similarity
    Binding sitei101 – 1011Quinone (QH)By similarity
    Metal bindingi106 – 1061Iron (heme b axial ligand)By similarity
    Metal bindingi284 – 2841CopperBy similarity
    Metal bindingi288 – 2881CopperBy similarity
    Metal bindingi333 – 3331CopperBy similarity
    Metal bindingi334 – 3341CopperBy similarity
    Metal bindingi419 – 4191Iron (heme o axial ligand)By similarity
    Metal bindingi421 – 4211Iron (heme b axial ligand)By similarity

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: InterPro
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor Source: InterPro

    GO - Biological processi

    1. aerobic respiration Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Hydrogen ion transport, Ion transport, Respiratory chain, Transport

    Keywords - Ligandi

    Copper, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-471-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome bo(3) ubiquinol oxidase subunit 1 (EC:1.10.3.10)
    Alternative name(s):
    Cytochrome o ubiquinol oxidase subunit 1
    Short name:
    Cytochrome o subunit 1
    Oxidase bo(3) subunit 1
    Ubiquinol oxidase polypeptide I
    Ubiquinol oxidase subunit 1
    Gene namesi
    Name:cyoB
    Ordered Locus Names:BU471
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    3. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 662662Cytochrome bo(3) ubiquinol oxidase subunit 1PRO_0000183479Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki284 ↔ 2881'-histidyl-3'-tyrosine (His-Tyr)By similarity

    Interactioni

    Subunit structurei

    Heterooctamer of two A chains, two B chains, two C chains and two D chains.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57543.
    SMRiP57543. Positions 52-552.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini36 – 5823CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini80 – 10627ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini128 – 14518CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini167 – 18923ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini211 – 23222CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini254 – 27724ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini299 – 30911CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini331 – 34616ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini368 – 38013CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini402 – 41312ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini435 – 45622CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini478 – 49316ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini515 – 58672CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini608 – 6081ExtracellularSequence Analysis
    Topological domaini630 – 66233CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei59 – 7921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei107 – 12721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei190 – 21021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei233 – 25321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei278 – 29821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 33021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei347 – 36721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei381 – 40121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei414 – 43421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei494 – 51421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei587 – 60721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei609 – 62921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0843.
    HOGENOMiHOG000085275.
    KOiK02298.
    OMAiMGMTRRV.
    OrthoDBiEOG6B35XR.

    Family and domain databases

    Gene3Di1.20.210.10. 1 hit.
    InterProiIPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    IPR014207. Cyt_c_ubiqinol_oxidase_su1.
    [Graphical view]
    PANTHERiPTHR10422. PTHR10422. 1 hit.
    PfamiPF00115. COX1. 1 hit.
    [Graphical view]
    PRINTSiPR01165. CYCOXIDASEI.
    SUPFAMiSSF81442. SSF81442. 1 hit.
    TIGRFAMsiTIGR02843. CyoB. 1 hit.
    PROSITEiPS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57543-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFGKLTFDAI PYHEPIIMIT YIAIILIALC IASTITYYKK WKYLWYEWFT    50
    TVDHKKISIM YGILAFVMLF RGFVDAILMR TQQVVASAGF KGFLPPHHYD 100
    QIFTAHGVIM IFFVAMPLVI GLMNLVIPLQ IGARDVAFPF LNNLSFWLNV 150
    SSAVLLTLSL GIGEFAQTGW LAYPPLSGIK YSSGVGVDYW IWSLQISGVG 200
    TTLTGINFLV TILKMRAPGM SFFKMPVFTW TSLCTNILIV ISFPVLTVTL 250
    VLLTLDRYFN FHFFTNDLGG NAMMYVNLIW IWGHPEVYIL VLPVFGVFSE 300
    VVATFSKKRL FGYVSLVWAT LSITILSFIV WLHHFFTMGA GADVNTFFGI 350
    TTMIIAIPTG VKIFNWLFTI YQGRVHMHSS ILWTLGFLVT FSIGGMTGVL 400
    LSVPPADFVL HNSLFLVAHF HNVIIGGVVF GCFAGINYWF PKLFGFVLNE 450
    IWGKRAFWFW IIGFFLAFIP LYFLGLMGMT RRLSQNIDSE FHMLLCIAAI 500
    GACFIGIGII CQVIQFFISI KERRHNLDLT GDPWDGRTLE WSTSSPAPLY 550
    NFAIIPKVED RDDFWRNKEG QHYNKLINSI NYHDIHMPKN TGLGFMISIF 600
    SLFFGFSAVW HITWLCILSF LAIIISLFIN SLNEDTEYTI SAEEIKKIEH 650
    QYWKNIQKAG LK 662
    Length:662
    Mass (Da):75,455
    Last modified:December 1, 2000 - v1
    Checksum:i3F502A628133EA65
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13168.1.
    RefSeqiNP_240282.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13168; BAB13168; BAB13168.
    GeneIDi1109818.
    KEGGibuc:BU471.
    PATRICi21244484. VBIBucAph127364_0480.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13168.1 .
    RefSeqi NP_240282.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57543.
    SMRi P57543. Positions 52-552.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13168 ; BAB13168 ; BAB13168 .
    GeneIDi 1109818.
    KEGGi buc:BU471.
    PATRICi 21244484. VBIBucAph127364_0480.

    Phylogenomic databases

    eggNOGi COG0843.
    HOGENOMi HOG000085275.
    KOi K02298.
    OMAi MGMTRRV.
    OrthoDBi EOG6B35XR.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-471-MONOMER.

    Family and domain databases

    Gene3Di 1.20.210.10. 1 hit.
    InterProi IPR000883. COX1.
    IPR023615. Cyt_c_Oxase_su1_BS.
    IPR023616. Cyt_c_Oxase_su1_dom.
    IPR014207. Cyt_c_ubiqinol_oxidase_su1.
    [Graphical view ]
    PANTHERi PTHR10422. PTHR10422. 1 hit.
    Pfami PF00115. COX1. 1 hit.
    [Graphical view ]
    PRINTSi PR01165. CYCOXIDASEI.
    SUPFAMi SSF81442. SSF81442. 1 hit.
    TIGRFAMsi TIGR02843. CyoB. 1 hit.
    PROSITEi PS50855. COX1. 1 hit.
    PS00077. COX1_CUB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiCYOB_BUCAI
    AccessioniPrimary (citable) accession number: P57543
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Ubiquinol oxidase catalyzes the terminal step in the electron transport chain.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3