P57540 (CYOE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoheme IX farnesyltransferase
EC=2.5.1.-

Alternative name(s):
Heme B farnesyltransferase
Heme O synthase

Gene names

Name:	cyoE
Ordered Locus Names:	BU468

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group By similarity. HAMAP-Rule MF_00154
Pathway	Porphyrin metabolism; heme O biosynthesis; heme O from protoheme: step 1/1. HAMAP-Rule MF_00154
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00154.
Miscellaneous	Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature By similarity. HAMAP-Rule MF_00154
Sequence similarities	Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme O biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protoheme IX farnesyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Protoheme IX farnesyltransferase HAMAP-Rule MF_00154		PRO_0000162898
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	36 – 56	21	Helical; Potential
	Transmembrane	80 – 100	21	Helical; Potential
	Transmembrane	107 – 127	21	Helical; Potential
	Transmembrane	133 – 153	21	Helical; Potential
	Transmembrane	163 – 183	21	Helical; Potential
	Transmembrane	209 – 229	21	Helical; Potential
	Transmembrane	232 – 252	21	Helical; Potential
	Transmembrane	265 – 285	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P57540 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 46504A297D4A16EF	FASTA	285	32,686
	10 20 30 40 50 60 MFKNYIEITK PGIIIGNSTL ITGGFLFASR HAAFNYVLFI YTILGASLVI ASACVFNNLI 70 80 90 100 110 120 DIDIDKKMKR TSNRVLSKKL LPVFSVFNFA IFLGVLGLSI LGCLVNFISM SLAVFGFFIY 130 140 150 160 170 180 VVLYTMFYKR RSFYSTFIGS FSGSTPSVIG YTAVSNTIDI CSILLFVIVI FWQMSHFYSI 190 200 210 220 230 240 SIMRIKDYRE AKIPVFSVVK GVAITKKHIF FYVLNFSFFS SLFTFLGYLS YNFLLLSSIV 250 260 270 280 NFYWSFLSYS NIKKNNDKKN ARKLFYFSII VIVFFNFLIS IDVFF

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13165.1.
RefSeq	NP_240279.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57540.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13165; BAB13165; BAB13165.
GeneID	1109815.
KEGG	buc:BU468.
PATRIC	21244478. VBIBucAph127364_0477.
Phylogenomic databases
eggNOG	COG0109.
HOGENOM	HOG000237290.
KO	K02301.
OMA	VGYCAVT.
ProtClustDB	PRK04375.
Enzyme and pathway databases
UniPathway	UPA00834; UER00712.
Family and domain databases
HAMAP	MF_00154. CyoE_CtaB.
InterPro	IPR006369. Protohaem_IX_farnesylTrfase. IPR000537. UbiA_prenyltransferase. [Graphical view]
Pfam	PF01040. UbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01473. cyoE_ctaB. 1 hit.
PROSITE	PS00943. UBIA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYOE_BUCAI

Accession

Primary (citable) accession number: P57540

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program